Click here to switch to the map view.

The map label for this gene is potD [H]

Identifier: 238924524

GI number: 238924524

Start: 2014157

End: 2016031

Strand: Direct

Name: potD [H]

Synonym: EUBREC_2166

Alternate gene names: 238924524

Gene position: 2014157-2016031 (Clockwise)

Preceding gene: 238924523

Following gene: 238924526

Centisome position: 58.39

GC content: 44.53

Gene sequence:

>1875_bases
ATGGTAAAAAAAATAGCATCAAGGGTATATATGGGACTGATTTTTTTGTTTCTGTATCTGCCTATCGTTGTATTAATAGT
ACTCTCCTTCAACAACTCCAAATCCAAGGTTAAATGGGGAGGCTTCACGCTCGACTGGTACATAAAGTGCTTTCAGAGTG
AGCGGATTATGTCGGCATTTTCTACAACACTGCAGATTACGCTGCTTGCAGCTGTGATTTCTACAATAATAGGAACTCTC
GCAGCCATGGGTATAAGCGCCATGAAGAAACGTAACCAGACAATCTATCTGGGCGCTACAAACATCCCGATGCTCAATGC
AGATATCGTGACCGGTATCTCAATGATGCTTTTGTTTGTAAAATTCATGAATCTGGGCTTTGTGACAGTGCTTATCGCAC
ACATCACGTTCAATATCCCTTATGTGATACTCAATGTGCTTCCAAAGCTCAAGCAGACCAACAAATACACCTATGAGGCC
GCACTTGACCTGGGTGCATCACCTCTATATGCATTTTTCAAGGTCACATGGCCTGAAATAAGCCCCGGAGTGTTCTCCGG
CTTTATGATGGCGGTCACAATGTCACTCGATGACTTCTCCATCACCTACTTTACGAAGGGTGCCGGAGTAAACACACTCT
CAACCATGCTCTACACAGAGCTGAAAAAGGGTGTAAAGCCGGAGCTGTATGCTCTGTCAACCATACTTTTCTTCACAGTG
CTGCTGCTTTTGGTTGTGGTAAACATCAACTCAAACCAGATACCTGTCAGCGCCTCAAAGAAGCCTGCAAGGGCTAAAAA
GCTGCGCATCGTAAAGCGTAGTGTGAGCATCGCAATTGTTGCAGTACTCGTAATCGGCTGCTTTTCGGTATTTACGATAA
GCGCCGGAAGCACGAACAATGACAATGCCATAACAGTGCTAAACTACGGTAAATACATCGATGAATCAGTAATCGACAGC
TTCGAGCAGGAAACCGGAATCACCATTAAATACGAGGAATACGAGGAGCCTGAGGAGATGTACACAAAGTACAAGTCCGG
TGCCATCGATTACGATGTCATCTGCACCTCTGACTACATCATCGAGAAGCTCATAAGTGAGGGTGAGGTCAACAAGATAG
ACTACAGCTCAATGCCAAACTATCAAAATGTAAATCAGGATATAATCGACATGTCCGCATCCTTTGACCCGACACATGAG
TATACTGTGCCTTATTTCTACGGCACACTCGGCATACTATACAATAAAAAGATGGCCGATGCATCAGACCTAAATACCTG
GGACTGCCTGTGGAACGGCAAATACAAGGACAACATGATTATGATTAATTCTGTCCGTGATGCCTTTACTCCCGCCCTTC
GCACACTTGGCTACTCGATAAACGAGACAGACACAGCAAAGCTTGATGAAGCATTTGATATACTGAACAAACAAAGCAGT
GATGTGCTCGCCTACTACGTGGATGAAACCTGTGATGAGATGGTTGCTGAAAATGCGGCAATCGCAGTGTGCTACTCCGG
TGAGGCAGCTGCCGCCATGGCTGAAAATGACAACCTGGACTATATCGTACCAAAAGAGGGCTCAAACCTCTGGATTGACT
CATGGTTTATTCCAAAGACATGCAAGAATAAAGAGGGCGCGCAGGAATTTTTGAACTATATCTGCAGTGATGAGCCGGCA
CAGCTCAACTTTGAATATGTATACTATGCATCACCTATCCAGTCTGTCATCGAAAACCAGGATGCAGAAACGAAGGAAAA
TGAGGCTATTAATCCGCCTTCCGATATGCTCAAAAACTGTGAAGTATACAGAGCTTTAAATGATGATGACGCAACACTTT
ACAACACACTGTGGCAGAGGCTCAAATCGGATTAA

Upstream 100 bases:

>100_bases
GGGGCGCAGGAAGCGGTCTCTCACTCGTGCTCATGGTATTCGTATTAATCAGCATGGCACTCGTCAATCTGTTTGATAAG
GAAGGAGACCAGAGCGCATT

Downstream 100 bases:

>100_bases
AAATGGATTGCGCAGCTATATTTTGTATGATGTCACAAAAATGAAGTCTAAAAATTGTACAAAAATACATATTGCAAACT
TCAACAAACAATGATAGAGT

Product: ornithine carbamoyltransferase

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MVKKIASRVYMGLIFLFLYLPIVVLIVLSFNNSKSKVKWGGFTLDWYIKCFQSERIMSAFSTTLQITLLAAVISTIIGTL
AAMGISAMKKRNQTIYLGATNIPMLNADIVTGISMMLLFVKFMNLGFVTVLIAHITFNIPYVILNVLPKLKQTNKYTYEA
ALDLGASPLYAFFKVTWPEISPGVFSGFMMAVTMSLDDFSITYFTKGAGVNTLSTMLYTELKKGVKPELYALSTILFFTV
LLLLVVVNINSNQIPVSASKKPARAKKLRIVKRSVSIAIVAVLVIGCFSVFTISAGSTNNDNAITVLNYGKYIDESVIDS
FEQETGITIKYEEYEEPEEMYTKYKSGAIDYDVICTSDYIIEKLISEGEVNKIDYSSMPNYQNVNQDIIDMSASFDPTHE
YTVPYFYGTLGILYNKKMADASDLNTWDCLWNGKYKDNMIMINSVRDAFTPALRTLGYSINETDTAKLDEAFDILNKQSS
DVLAYYVDETCDEMVAENAAIAVCYSGEAAAAMAENDNLDYIVPKEGSNLWIDSWFIPKTCKNKEGAQEFLNYICSDEPA
QLNFEYVYYASPIQSVIENQDAETKENEAINPPSDMLKNCEVYRALNDDDATLYNTLWQRLKSD

Sequences:

>Translated_624_residues
MVKKIASRVYMGLIFLFLYLPIVVLIVLSFNNSKSKVKWGGFTLDWYIKCFQSERIMSAFSTTLQITLLAAVISTIIGTL
AAMGISAMKKRNQTIYLGATNIPMLNADIVTGISMMLLFVKFMNLGFVTVLIAHITFNIPYVILNVLPKLKQTNKYTYEA
ALDLGASPLYAFFKVTWPEISPGVFSGFMMAVTMSLDDFSITYFTKGAGVNTLSTMLYTELKKGVKPELYALSTILFFTV
LLLLVVVNINSNQIPVSASKKPARAKKLRIVKRSVSIAIVAVLVIGCFSVFTISAGSTNNDNAITVLNYGKYIDESVIDS
FEQETGITIKYEEYEEPEEMYTKYKSGAIDYDVICTSDYIIEKLISEGEVNKIDYSSMPNYQNVNQDIIDMSASFDPTHE
YTVPYFYGTLGILYNKKMADASDLNTWDCLWNGKYKDNMIMINSVRDAFTPALRTLGYSINETDTAKLDEAFDILNKQSS
DVLAYYVDETCDEMVAENAAIAVCYSGEAAAAMAENDNLDYIVPKEGSNLWIDSWFIPKTCKNKEGAQEFLNYICSDEPA
QLNFEYVYYASPIQSVIENQDAETKENEAINPPSDMLKNCEVYRALNDDDATLYNTLWQRLKSD
>Mature_624_residues
MVKKIASRVYMGLIFLFLYLPIVVLIVLSFNNSKSKVKWGGFTLDWYIKCFQSERIMSAFSTTLQITLLAAVISTIIGTL
AAMGISAMKKRNQTIYLGATNIPMLNADIVTGISMMLLFVKFMNLGFVTVLIAHITFNIPYVILNVLPKLKQTNKYTYEA
ALDLGASPLYAFFKVTWPEISPGVFSGFMMAVTMSLDDFSITYFTKGAGVNTLSTMLYTELKKGVKPELYALSTILFFTV
LLLLVVVNINSNQIPVSASKKPARAKKLRIVKRSVSIAIVAVLVIGCFSVFTISAGSTNNDNAITVLNYGKYIDESVIDS
FEQETGITIKYEEYEEPEEMYTKYKSGAIDYDVICTSDYIIEKLISEGEVNKIDYSSMPNYQNVNQDIIDMSASFDPTHE
YTVPYFYGTLGILYNKKMADASDLNTWDCLWNGKYKDNMIMINSVRDAFTPALRTLGYSINETDTAKLDEAFDILNKQSS
DVLAYYVDETCDEMVAENAAIAVCYSGEAAAAMAENDNLDYIVPKEGSNLWIDSWFIPKTCKNKEGAQEFLNYICSDEPA
QLNFEYVYYASPIQSVIENQDAETKENEAINPPSDMLKNCEVYRALNDDDATLYNTLWQRLKSD

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=336, Percent_Identity=30.952380952381, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI1787081, Length=234, Percent_Identity=35.4700854700855, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1787368, Length=218, Percent_Identity=33.4862385321101, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI1787078, Length=364, Percent_Identity=23.6263736263736, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1787714, Length=215, Percent_Identity=30.6976744186047, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 70031; Mature: 70031

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKIASRVYMGLIFLFLYLPIVVLIVLSFNNSKSKVKWGGFTLDWYIKCFQSERIMSAF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEHHHHHHHHHHHHHHHHH
STTLQITLLAAVISTIIGTLAAMGISAMKKRNQTIYLGATNIPMLNADIVTGISMMLLFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECHHHHHHHHHHHHHH
KFMNLGFVTVLIAHITFNIPYVILNVLPKLKQTNKYTYEAALDLGASPLYAFFKVTWPEI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCEEEEEEECCCC
SPGVFSGFMMAVTMSLDDFSITYFTKGAGVNTLSTMLYTELKKGVKPELYALSTILFFTV
CCHHHHHHHHHHEEECCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LLLLVVVNINSNQIPVSASKKPARAKKLRIVKRSVSIAIVAVLVIGCFSVFTISAGSTNN
HHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCC
DNAITVLNYGKYIDESVIDSFEQETGITIKYEEYEEPEEMYTKYKSGAIDYDVICTSDYI
CCEEEEEECCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCEEEEECCHHH
IEKLISEGEVNKIDYSSMPNYQNVNQDIIDMSASFDPTHEYTVPYFYGTLGILYNKKMAD
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEECCHHHHHHHHHCCCCCCC
ASDLNTWDCLWNGKYKDNMIMINSVRDAFTPALRTLGYSINETDTAKLDEAFDILNKQSS
CCCCCCEEEEECCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
DVLAYYVDETCDEMVAENAAIAVCYSGEAAAAMAENDNLDYIVPKEGSNLWIDSWFIPKT
CEEEEEHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCEEEECCCCCEEEEEEECCCC
CKNKEGAQEFLNYICSDEPAQLNFEYVYYASPIQSVIENQDAETKENEAINPPSDMLKNC
CCCCCHHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHCCCCCCCCCCCCCCCHHHHHHCC
EVYRALNDDDATLYNTLWQRLKSD
HHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MVKKIASRVYMGLIFLFLYLPIVVLIVLSFNNSKSKVKWGGFTLDWYIKCFQSERIMSAF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEHHHHHHHHHHHHHHHHH
STTLQITLLAAVISTIIGTLAAMGISAMKKRNQTIYLGATNIPMLNADIVTGISMMLLFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECHHHHHHHHHHHHHH
KFMNLGFVTVLIAHITFNIPYVILNVLPKLKQTNKYTYEAALDLGASPLYAFFKVTWPEI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCEEEEEEECCCC
SPGVFSGFMMAVTMSLDDFSITYFTKGAGVNTLSTMLYTELKKGVKPELYALSTILFFTV
CCHHHHHHHHHHEEECCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LLLLVVVNINSNQIPVSASKKPARAKKLRIVKRSVSIAIVAVLVIGCFSVFTISAGSTNN
HHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCC
DNAITVLNYGKYIDESVIDSFEQETGITIKYEEYEEPEEMYTKYKSGAIDYDVICTSDYI
CCEEEEEECCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCEEEEECCHHH
IEKLISEGEVNKIDYSSMPNYQNVNQDIIDMSASFDPTHEYTVPYFYGTLGILYNKKMAD
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEECCHHHHHHHHHCCCCCCC
ASDLNTWDCLWNGKYKDNMIMINSVRDAFTPALRTLGYSINETDTAKLDEAFDILNKQSS
CCCCCCEEEEECCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
DVLAYYVDETCDEMVAENAAIAVCYSGEAAAAMAENDNLDYIVPKEGSNLWIDSWFIPKT
CEEEEEHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCEEEECCCCCEEEEEEECCCC
CKNKEGAQEFLNYICSDEPAQLNFEYVYYASPIQSVIENQDAETKENEAINPPSDMLKNC
CCCCCHHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHCCCCCCCCCCCCCCCHHHHHHCC
EVYRALNDDDATLYNTLWQRLKSD
HHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]