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The map label for this gene is nudE [H]

Identifier: 238921480

GI number: 238921480

Start: 3518852

End: 3519307

Strand: Reverse

Name: nudE [H]

Synonym: NT01EI_3632

Alternate gene names: 238921480

Gene position: 3519307-3518852 (Counterclockwise)

Preceding gene: 238921490

Following gene: 238921479

Centisome position: 92.31

GC content: 59.65

Gene sequence:

>456_bases
GTGCGCCGGGTGTATGAGCGTATGCGTCCGTCAGGCCTCGAGGCGGTGATGATCGTTCCTATACTGGGGGACGATCTGCT
ACTGATCCGGGAATATGCGGTCGGCATTGAGGAGTATGAACTGGGTTTTCCCAAGGGGCTTATCGATCCCGGTGAGCGGG
TGTTCCAGGCGGCCAACCGTGAGCTGATGGAAGAAGCCGGCTTTGGGGCGCAGCGTTTTACCCTGCTGCATAAGCTCACG
ATGGCCCCCTCCTATTTCTCCAGTCAGATGAACGTGGTGGTAGCAGAAAATCTGTTCGCTCAGCGCCTGCAGGGCGATGA
GCCTGAGATGATGCCGGTGATCCGCTGGCCGCTGGACCAGATGATGGCACTGCTGCAGGAGCCCGATTTTCACGAGGCCC
GCAACGTGAGCGCGCTGTTTTTGCTGCGGGAGTTTCTACTGCGCCGCACCGCCTAG

Upstream 100 bases:

>100_bases
GGATAGTTCCACTAAGAAGCCCAGGATCTTGGGCGTTGAAACCGTGGCGCACTCACGCTTATTTAACGTCGAGTCCGTTG
ATTTGGAGTTTAGCAACGGG

Downstream 100 bases:

>100_bases
GACGATGTGAGTGAATGTGGATAAAAGGGAGCCTGGTAGCCTGCCCCCAGAAACATGGGCAGTACACTGTGATGTGTTTC
TGTAGTTCATGCTATGTCAG

Product: ADP-ribose diphosphatase NudE

Products: AMP; D-Ribose 5- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MRRVYERMRPSGLEAVMIVPILGDDLLLIREYAVGIEEYELGFPKGLIDPGERVFQAANRELMEEAGFGAQRFTLLHKLT
MAPSYFSSQMNVVVAENLFAQRLQGDEPEMMPVIRWPLDQMMALLQEPDFHEARNVSALFLLREFLLRRTA

Sequences:

>Translated_151_residues
MRRVYERMRPSGLEAVMIVPILGDDLLLIREYAVGIEEYELGFPKGLIDPGERVFQAANRELMEEAGFGAQRFTLLHKLT
MAPSYFSSQMNVVVAENLFAQRLQGDEPEMMPVIRWPLDQMMALLQEPDFHEARNVSALFLLREFLLRRTA
>Mature_151_residues
MRRVYERMRPSGLEAVMIVPILGDDLLLIREYAVGIEEYELGFPKGLIDPGERVFQAANRELMEEAGFGAQRFTLLHKLT
MAPSYFSSQMNVVVAENLFAQRLQGDEPEMMPVIRWPLDQMMALLQEPDFHEARNVSALFLLREFLLRRTA

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=146, Percent_Identity=70.5479452054795, Blast_Score=219, Evalue=7e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 17418; Mature: 17418

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.6 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRVYERMRPSGLEAVMIVPILGDDLLLIREYAVGIEEYELGFPKGLIDPGERVFQAANR
CCHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHH
ELMEEAGFGAQRFTLLHKLTMAPSYFSSQMNVVVAENLFAQRLQGDEPEMMPVIRWPLDQ
HHHHHCCCCHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHCCHHH
MMALLQEPDFHEARNVSALFLLREFLLRRTA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRRVYERMRPSGLEAVMIVPILGDDLLLIREYAVGIEEYELGFPKGLIDPGERVFQAANR
CCHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHH
ELMEEAGFGAQRFTLLHKLTMAPSYFSSQMNVVVAENLFAQRLQGDEPEMMPVIRWPLDQ
HHHHHCCCCHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHCCHHH
MMALLQEPDFHEARNVSALFLLREFLLRRTA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ADP-Ribose; H2O [C]

Specific reaction: ADP-Ribose + H2O = AMP + D-Ribose 5- Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]