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The map label for this gene is pyrB [H]

Identifier: 238921334

GI number: 238921334

Start: 3372872

End: 3373807

Strand: Reverse

Name: pyrB [H]

Synonym: NT01EI_3478

Alternate gene names: 238921334

Gene position: 3373807-3372872 (Counterclockwise)

Preceding gene: 238921335

Following gene: 238921333

Centisome position: 88.5

GC content: 59.4

Gene sequence:

>936_bases
ATGGCTAATCCCTTGTATCTAAAAGATATTGTCTCCATCAACGATCTGAGTCGCCGCGAGCTTGAGCTGGTACTTGATAC
CGCCGCCCGCCTGAAGGCCACGCCGCAGCCGGAGCTGCTGCGCCACAAGGTGATCGCCAGCCTGTTCTTTGAGGCCTCCA
CCCGTACCCGCCTCTCTTTTGAAACCGCCATGCACCGCCTGGGCGCCTCGGTGGTCGGCTTTGCCGACAGCAGCAACACC
TCGCTGGGCAAGAAGGGGGAGACGCTGGCGGATACCATCTCGGTGATCGGCACCTATGTCGACGCCATCGTGATGCGTCA
CCCGCAGGAGGGGGCCGCCCGTCTGGCCACGGAGTTCTGCGGCCGCATCCCGGTGGTCAACGCCGGTGACGGCGCCAACC
AGCACCCGACCCAGACGCTGCTGGATCTATTTACTATTCAGGAGACCCAGGGGCGCCTGAATAACATCAATATCGCCATG
GTTGGCGACCTGAAGTATGGGCGCACCGTGCACTCCCTGACTCAGGCGCTGGCCAAGTTTGAGGGTAACCGCTTCTTCTT
CATCGCTCCGCAGGCCCTGGCGATGCCGGATTATATTCTGACCATGCTGGAGGAGAAGGGGATCGCCTACAGCCTGCATG
AAACCATTGAAGAGGTGATTCCACAGCTGGATATTCTGTATATGACCCGGGTACAGAAAGAGCGTCTGGATCCCTCCGAA
TACGCCAACGTTAAGTCGCAGTTCATTCTGCGCGCCACGATGCTGGAGGGAGCGCGCGATAACCTGAAAGTGCTGCATCC
GCTGCCGCGTATCGACGAGATCACCCCCGACGTCGATGTCACCCCCTATGCCTACTATTTCCAGCAGGCGGGCAACGGTA
TCTTCGCCCGTCAGGCGCTGCTGGCCCTGATCCTCAACGCTGAACTGAATTTCTAA

Upstream 100 bases:

>100_bases
TCATTTGAGGGTTTTTTTTTGCCCATAATCCGGCAAGTTGAAAAATTAACCCGAGTGAACAGGATGCCCGCGTCGCTGGC
GTCAGAATAGGAGAGCACCA

Downstream 100 bases:

>100_bases
GGGAGAAACGAAATGACTCACGACAACAAATTACAGGTTGAAGCGATTAAATGCGGCACCGTCATTGACCATATCCCGGC
GCACGTTGGCTTTAAGCTGC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MANPLYLKDIVSINDLSRRELELVLDTAARLKATPQPELLRHKVIASLFFEASTRTRLSFETAMHRLGASVVGFADSSNT
SLGKKGETLADTISVIGTYVDAIVMRHPQEGAARLATEFCGRIPVVNAGDGANQHPTQTLLDLFTIQETQGRLNNINIAM
VGDLKYGRTVHSLTQALAKFEGNRFFFIAPQALAMPDYILTMLEEKGIAYSLHETIEEVIPQLDILYMTRVQKERLDPSE
YANVKSQFILRATMLEGARDNLKVLHPLPRIDEITPDVDVTPYAYYFQQAGNGIFARQALLALILNAELNF

Sequences:

>Translated_311_residues
MANPLYLKDIVSINDLSRRELELVLDTAARLKATPQPELLRHKVIASLFFEASTRTRLSFETAMHRLGASVVGFADSSNT
SLGKKGETLADTISVIGTYVDAIVMRHPQEGAARLATEFCGRIPVVNAGDGANQHPTQTLLDLFTIQETQGRLNNINIAM
VGDLKYGRTVHSLTQALAKFEGNRFFFIAPQALAMPDYILTMLEEKGIAYSLHETIEEVIPQLDILYMTRVQKERLDPSE
YANVKSQFILRATMLEGARDNLKVLHPLPRIDEITPDVDVTPYAYYFQQAGNGIFARQALLALILNAELNF
>Mature_310_residues
ANPLYLKDIVSINDLSRRELELVLDTAARLKATPQPELLRHKVIASLFFEASTRTRLSFETAMHRLGASVVGFADSSNTS
LGKKGETLADTISVIGTYVDAIVMRHPQEGAARLATEFCGRIPVVNAGDGANQHPTQTLLDLFTIQETQGRLNNINIAMV
GDLKYGRTVHSLTQALAKFEGNRFFFIAPQALAMPDYILTMLEEKGIAYSLHETIEEVIPQLDILYMTRVQKERLDPSEY
ANVKSQFILRATMLEGARDNLKVLHPLPRIDEITPDVDVTPYAYYFQQAGNGIFARQALLALILNAELNF

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=304, Percent_Identity=43.75, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI38788445, Length=314, Percent_Identity=27.0700636942675, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=82.2006472491909, Blast_Score=539, Evalue=1e-155,
Organism=Escherichia coli, GI1790703, Length=281, Percent_Identity=29.8932384341637, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786469, Length=288, Percent_Identity=30.9027777777778, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=323, Percent_Identity=39.938080495356, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6322331, Length=315, Percent_Identity=40, Blast_Score=228, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24642586, Length=313, Percent_Identity=41.5335463258786, Blast_Score=241, Evalue=4e-64,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34637; Mature: 34506

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00097 CARBAMOYLTRANSFERASE ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANPLYLKDIVSINDLSRRELELVLDTAARLKATPQPELLRHKVIASLFFEASTRTRLSF
CCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
ETAMHRLGASVVGFADSSNTSLGKKGETLADTISVIGTYVDAIVMRHPQEGAARLATEFC
HHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GRIPVVNAGDGANQHPTQTLLDLFTIQETQGRLNNINIAMVGDLKYGRTVHSLTQALAKF
CCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
EGNRFFFIAPQALAMPDYILTMLEEKGIAYSLHETIEEVIPQLDILYMTRVQKERLDPSE
CCCEEEEECCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
YANVKSQFILRATMLEGARDNLKVLHPLPRIDEITPDVDVTPYAYYFQQAGNGIFARQAL
HHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHH
LALILNAELNF
HHHHHHHCCCC
>Mature Secondary Structure 
ANPLYLKDIVSINDLSRRELELVLDTAARLKATPQPELLRHKVIASLFFEASTRTRLSF
CCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
ETAMHRLGASVVGFADSSNTSLGKKGETLADTISVIGTYVDAIVMRHPQEGAARLATEFC
HHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GRIPVVNAGDGANQHPTQTLLDLFTIQETQGRLNNINIAMVGDLKYGRTVHSLTQALAKF
CCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
EGNRFFFIAPQALAMPDYILTMLEEKGIAYSLHETIEEVIPQLDILYMTRVQKERLDPSE
CCCEEEEECCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
YANVKSQFILRATMLEGARDNLKVLHPLPRIDEITPDVDVTPYAYYFQQAGNGIFARQAL
HHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHH
LALILNAELNF
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA