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The map label for this gene is pyrI

Identifier: 238921333

GI number: 238921333

Start: 3372398

End: 3372859

Strand: Reverse

Name: pyrI

Synonym: NT01EI_3477

Alternate gene names: 238921333

Gene position: 3372859-3372398 (Counterclockwise)

Preceding gene: 238921334

Following gene: 238921332

Centisome position: 88.47

GC content: 56.28

Gene sequence:

>462_bases
ATGACTCACGACAACAAATTACAGGTTGAAGCGATTAAATGCGGCACCGTCATTGACCATATCCCGGCGCACGTTGGCTT
TAAGCTGCTGACCCTGTTCAAGCTGACGGAGACCGATCGGCGGGTCACCATCGGCCTGAATCTGCCCTCTAACGCACAGG
GCAGCAAGGATCTGATCAAGATTGAGGATGTCTTTCTCAGCGAGGAGCAGGTGAACCAGCTGGCGATTTACGCCCCGCGC
GCTACGGTCAACTGCATTGAGAACTACAGCGTGGTGCGCAAGATGGTGCCGAGCCTGCCGGAGCGCATCGACAGCGTACT
GACCTGTCCCAACAGTAACTGCATCAGCCGCAGCGAACCGGTTTGCTCCAGCTTCAGCGTGCGCGCCCGTGCCGGTGAGG
TACTGCTTAAGTGCAAGTATTGTGAGAAAGAGTTTGCGCATCACGCCGTGATGGAACAGTGA

Upstream 100 bases:

>100_bases
TGCCTACTATTTCCAGCAGGCGGGCAACGGTATCTTCGCCCGTCAGGCGCTGCTGGCCCTGATCCTCAACGCTGAACTGA
ATTTCTAAGGGAGAAACGAA

Downstream 100 bases:

>100_bases
TCCGGCAGGGTGGGATTTGCCGTGCGGGCTCCTTATAATGGGGCCTGGTTACCGTAGTCCCGCGGTAACGTCACATCCTA
CCATCCTTAAGGAGCAGATA

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MTHDNKLQVEAIKCGTVIDHIPAHVGFKLLTLFKLTETDRRVTIGLNLPSNAQGSKDLIKIEDVFLSEEQVNQLAIYAPR
ATVNCIENYSVVRKMVPSLPERIDSVLTCPNSNCISRSEPVCSSFSVRARAGEVLLKCKYCEKEFAHHAVMEQ

Sequences:

>Translated_153_residues
MTHDNKLQVEAIKCGTVIDHIPAHVGFKLLTLFKLTETDRRVTIGLNLPSNAQGSKDLIKIEDVFLSEEQVNQLAIYAPR
ATVNCIENYSVVRKMVPSLPERIDSVLTCPNSNCISRSEPVCSSFSVRARAGEVLLKCKYCEKEFAHHAVMEQ
>Mature_152_residues
THDNKLQVEAIKCGTVIDHIPAHVGFKLLTLFKLTETDRRVTIGLNLPSNAQGSKDLIKIEDVFLSEEQVNQLAIYAPRA
TVNCIENYSVVRKMVPSLPERIDSVLTCPNSNCISRSEPVCSSFSVRARAGEVLLKCKYCEKEFAHHAVMEQ

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family

Homologues:

Organism=Escherichia coli, GI1790692, Length=151, Percent_Identity=75.4966887417219, Blast_Score=243, Evalue=4e-66,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRI_EDWI9 (C5BC06)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934848.1
- GeneID:   7962475
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3477
- OMA:   EGTVIDH
- ProtClustDB:   PRK00893
- HAMAP:   MF_00002
- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- Gene3D:   G3DSA:2.30.30.20
- Gene3D:   G3DSA:3.30.70.140
- ProDom:   PD006194
- TIGRFAMs:   TIGR00240

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C; SSF54893 Asp_carbamoylTrfase_reg; SSF57825 Asp_transf_reg_C

EC number: 2.1.3.2

Molecular weight: Translated: 17102; Mature: 16971

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
4.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHDNKLQVEAIKCGTVIDHIPAHVGFKLLTLFKLTETDRRVTIGLNLPSNAQGSKDLIK
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEE
IEDVFLSEEQVNQLAIYAPRATVNCIENYSVVRKMVPSLPERIDSVLTCPNSNCISRSEP
HHHHHCCHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCC
VCSSFSVRARAGEVLLKCKYCEKEFAHHAVMEQ
CCCCCCCHHCCCCEEEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure 
THDNKLQVEAIKCGTVIDHIPAHVGFKLLTLFKLTETDRRVTIGLNLPSNAQGSKDLIK
CCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEE
IEDVFLSEEQVNQLAIYAPRATVNCIENYSVVRKMVPSLPERIDSVLTCPNSNCISRSEP
HHHHHCCHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCC
VCSSFSVRARAGEVLLKCKYCEKEFAHHAVMEQ
CCCCCCCHHCCCCEEEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA