| Definition | Edwardsiella ictaluri 93-146 chromosome, complete genome. |
|---|---|
| Accession | NC_012779 |
| Length | 3,812,315 |
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The map label for this gene is hsdM [H]
Identifier: 238921301
GI number: 238921301
Start: 3334832
End: 3337270
Strand: Reverse
Name: hsdM [H]
Synonym: NT01EI_3444
Alternate gene names: 238921301
Gene position: 3337270-3334832 (Counterclockwise)
Preceding gene: 238921302
Following gene: 238921300
Centisome position: 87.54
GC content: 56.17
Gene sequence:
>2439_bases ATGGCTATCAAAAAAACCGAACTCTATTCCTCTTTATGGGCAAGCTGCGACGAACTGCGCGGTGGGATGGATGCCAGCCA GTACAAGGACTATGTACTGACCCTGCTGTTTATGAAGTATGTGTCCGACAAATATAAGGGCGATCCCTACGGGATGATCG TGATCCCGAAAGGGGCCAGCTTTGACGATATGGTCGCGCTGAAGAACGATAAAGAAATCGGCGATAAAATTAACAAAATC ATCCGCAGGCTGGCGGAAGAGAACGATTTGAAAGGCGTTATCGACGAGGCCGACTTTAACGATGAGGACAAGCTCGGCAA AGGTAAGGAGATGATGGATCGCCTGACCAAGCTGGTTGGTATCTTCGAGGGGCTGGATCTGTCCGGCAACCGGGCCGATG GCGACGATCTGCTGGGCGATGCCTATGAGTACCTGATGCGTCACTTTGCCACCGAATCCGGTAAAAGCAAGGGGCAGTTC TACACGCCTGCGGAGGTCTCGCGTATTCTGGCGAAGGTGATCGGCATTACGCCCGATACGCCACAGGATGCCACGGTCTA CGACCCCACCTGTGGCTCCGGTTCGTTGTTGCTTAAAGTCAGCGATGAAACCCGACGCGGGCTGTCGATATTCGGGCAGG AGATGGACAACGCTACCAGCGCGCTGGCGCGGATGAACATGATCCTGCATAACAACGCCACCGCTAAAATCTGGCAGGGC AATACCCTGAGCGATCCACAGTGGAAAGTGGCCAACGGCAAACTGAAAACCTTCGACTTCGCCGTGGCGAACCCGCCGTT TTCCAATAAAAACTGGACCAGCGGGCTGGATCTGAAGAGAGATCCGTTTGAGCGCTTCGTCTGGGGCGTTCCACCGGAGA AGAACGGCGATTATGCCTTTCTGCTGCACATCATCAAGAGCCTGAAAAGCACCGGCAAAGGGGCGGTGATCCTGCCACAC GGCGTGCTGTTCCGCGGTAACGCCGAAGCGAATATCCGCGAAAACCTGATCAAGCAAGGCTATATCAAAGGCATTATCGG CCTACCGGCTAACCTGTTTTACGGTACCGGCATCCCGGCGTGCATTATCGTGATTGATAAAGAGCACGCCCACAGCCGCA AAGGCATTTTTATGATCGATGCCAGCCGGGGCTTTATCAAAGATGGCAACAAGAACCGCCTGCGTAGCCGGGATATTCAC CGCATCGTGGATGTGTTTAACCATCAGCGAACGCTGCCCGGCTACAGCCGGATGGTGCCATTAAGTGACATCGCCGGGAA TGATTACAACCTGAATATCCCCCGCTATATCGACGGCGGCGAGCCGGAAGATTTGCACGATCTGAGCGCCCATTTACAGG GCGGTATTCCGGCGCGCGATGTGGATGCCCTGCAGGATTACTGGCGGGTATTTCCTGCCCTGCGCGACGTGCTGTTTACC GACGATCGCCCCGGCTACTGTCGGGCGCAGGTCGAAGCCCAACAGGTTAAACCGACCATTCTGGCGCATCAGGAATTTAA GGATTTTTCCACCCGCAGCCTGCTGCCGTTTAAGGCGTGGGTGAAAGAGGCGGCACTGAATGAGATCGGTAAGGGCGATA AACCCAAGGCGCTGATCCACGATATCAGCGAGATGCTGCTGGCGCAGTATGCCAACAGCGAGCTGCTGAATAAATACAGC GTGTACCAGATCCTGATGGACTACTGGGCCGACACCATGCAGGACGATGTCTACGTGATCATGCAGGACGGCTGGCAAGC CGCCGCGCACATTCGCGAACTCCAGCCGGTGAAAGGTAAGGATGGCAAAAACGTCTGGAAAGAGGCGCATGATTTTGAGT TTGACAAGCGGCGCTACAAGGCCGACGTGCTGCCCCGTTCTTTGGTGGAGATTCGCTGTTTCCCGCAACAGCTGGAGGCG CTGAACCGCGCACAGGAGCGCAGTGAAGAAGCCAGCCGTCAGTTAGCCGAGTTTATCGAAGAGCAGACCGGGGAAGAGGA TCTGCTGGCCGAGGCGAAGAACGACAAGGATAAGGTGACGCAAAAGCTGGTCAATGCCCGCCTGACAAAATTGAAGAAAA CCACCGCCGATCCGGATGAGCTGGCGGCGCTGACCCGCTGCCTGGCGCTGATTAAAGCAGAGGCCAATGCGAAAAAAGCG CTGAAAGTCGCACAGGATGCGCTGGATCGCGCGGTGTTTAAACAGTATCCCACGCTGGACGAACCGGCGATCAAGGCGCT GGTGGTGCAGGACAAATGGCTGGCGACGTTGCAGGCCGGGATTAAGGCGGAGATTGAGCGCATCACCCAGCAACTGGCGG AGCGCGTAAAGGAGCTGGAAGAGCGTTATGCCGAGCCGTTACCGGCGCTGGAGGCGTCCGTTGAAGCGTTAAGCAAGAAG GTGGCCGGGCATTTGCGGGCGATGGGGCTGGTGTGGTGA
Upstream 100 bases:
>100_bases GCAAAATGCGTAACTTAACCCGTCGGCTGAAAGCCGCCGGACTGAACACCGATCTGCTGTAATCGTTTACAGCACAGAAT CGCGCGAAACGAGATATTTA
Downstream 100 bases:
>100_bases TGGCTATGGTGGATTTGGTGGTGGATAAACAGCGGTCGGGCGCGGCGAGAAAAGGCGTGCCTGCGGGGTTTAAGTTGACT GAGGTGGGGGTTATTCCTGA
Product: type I restriction enzyme M protein (HsdM)
Products: NA
Alternate protein names: M.EcoR124II [H]
Number of amino acids: Translated: 812; Mature: 811
Protein sequence:
>812_residues MAIKKTELYSSLWASCDELRGGMDASQYKDYVLTLLFMKYVSDKYKGDPYGMIVIPKGASFDDMVALKNDKEIGDKINKI IRRLAEENDLKGVIDEADFNDEDKLGKGKEMMDRLTKLVGIFEGLDLSGNRADGDDLLGDAYEYLMRHFATESGKSKGQF YTPAEVSRILAKVIGITPDTPQDATVYDPTCGSGSLLLKVSDETRRGLSIFGQEMDNATSALARMNMILHNNATAKIWQG NTLSDPQWKVANGKLKTFDFAVANPPFSNKNWTSGLDLKRDPFERFVWGVPPEKNGDYAFLLHIIKSLKSTGKGAVILPH GVLFRGNAEANIRENLIKQGYIKGIIGLPANLFYGTGIPACIIVIDKEHAHSRKGIFMIDASRGFIKDGNKNRLRSRDIH RIVDVFNHQRTLPGYSRMVPLSDIAGNDYNLNIPRYIDGGEPEDLHDLSAHLQGGIPARDVDALQDYWRVFPALRDVLFT DDRPGYCRAQVEAQQVKPTILAHQEFKDFSTRSLLPFKAWVKEAALNEIGKGDKPKALIHDISEMLLAQYANSELLNKYS VYQILMDYWADTMQDDVYVIMQDGWQAAAHIRELQPVKGKDGKNVWKEAHDFEFDKRRYKADVLPRSLVEIRCFPQQLEA LNRAQERSEEASRQLAEFIEEQTGEEDLLAEAKNDKDKVTQKLVNARLTKLKKTTADPDELAALTRCLALIKAEANAKKA LKVAQDALDRAVFKQYPTLDEPAIKALVVQDKWLATLQAGIKAEIERITQQLAERVKELEERYAEPLPALEASVEALSKK VAGHLRAMGLVW
Sequences:
>Translated_812_residues MAIKKTELYSSLWASCDELRGGMDASQYKDYVLTLLFMKYVSDKYKGDPYGMIVIPKGASFDDMVALKNDKEIGDKINKI IRRLAEENDLKGVIDEADFNDEDKLGKGKEMMDRLTKLVGIFEGLDLSGNRADGDDLLGDAYEYLMRHFATESGKSKGQF YTPAEVSRILAKVIGITPDTPQDATVYDPTCGSGSLLLKVSDETRRGLSIFGQEMDNATSALARMNMILHNNATAKIWQG NTLSDPQWKVANGKLKTFDFAVANPPFSNKNWTSGLDLKRDPFERFVWGVPPEKNGDYAFLLHIIKSLKSTGKGAVILPH GVLFRGNAEANIRENLIKQGYIKGIIGLPANLFYGTGIPACIIVIDKEHAHSRKGIFMIDASRGFIKDGNKNRLRSRDIH RIVDVFNHQRTLPGYSRMVPLSDIAGNDYNLNIPRYIDGGEPEDLHDLSAHLQGGIPARDVDALQDYWRVFPALRDVLFT DDRPGYCRAQVEAQQVKPTILAHQEFKDFSTRSLLPFKAWVKEAALNEIGKGDKPKALIHDISEMLLAQYANSELLNKYS VYQILMDYWADTMQDDVYVIMQDGWQAAAHIRELQPVKGKDGKNVWKEAHDFEFDKRRYKADVLPRSLVEIRCFPQQLEA LNRAQERSEEASRQLAEFIEEQTGEEDLLAEAKNDKDKVTQKLVNARLTKLKKTTADPDELAALTRCLALIKAEANAKKA LKVAQDALDRAVFKQYPTLDEPAIKALVVQDKWLATLQAGIKAEIERITQQLAERVKELEERYAEPLPALEASVEALSKK VAGHLRAMGLVW >Mature_811_residues AIKKTELYSSLWASCDELRGGMDASQYKDYVLTLLFMKYVSDKYKGDPYGMIVIPKGASFDDMVALKNDKEIGDKINKII RRLAEENDLKGVIDEADFNDEDKLGKGKEMMDRLTKLVGIFEGLDLSGNRADGDDLLGDAYEYLMRHFATESGKSKGQFY TPAEVSRILAKVIGITPDTPQDATVYDPTCGSGSLLLKVSDETRRGLSIFGQEMDNATSALARMNMILHNNATAKIWQGN TLSDPQWKVANGKLKTFDFAVANPPFSNKNWTSGLDLKRDPFERFVWGVPPEKNGDYAFLLHIIKSLKSTGKGAVILPHG VLFRGNAEANIRENLIKQGYIKGIIGLPANLFYGTGIPACIIVIDKEHAHSRKGIFMIDASRGFIKDGNKNRLRSRDIHR IVDVFNHQRTLPGYSRMVPLSDIAGNDYNLNIPRYIDGGEPEDLHDLSAHLQGGIPARDVDALQDYWRVFPALRDVLFTD DRPGYCRAQVEAQQVKPTILAHQEFKDFSTRSLLPFKAWVKEAALNEIGKGDKPKALIHDISEMLLAQYANSELLNKYSV YQILMDYWADTMQDDVYVIMQDGWQAAAHIRELQPVKGKDGKNVWKEAHDFEFDKRRYKADVLPRSLVEIRCFPQQLEAL NRAQERSEEASRQLAEFIEEQTGEEDLLAEAKNDKDKVTQKLVNARLTKLKKTTADPDELAALTRCLALIKAEANAKKAL KVAQDALDRAVFKQYPTLDEPAIKALVVQDKWLATLQAGIKAEIERITQQLAERVKELEERYAEPLPALEASVEALSKKV AGHLRAMGLVW
Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]
COG id: COG0286
COG function: function code V; Type I restriction-modification system methyltransferase subunit
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]
Homologues:
Organism=Escherichia coli, GI1790808, Length=446, Percent_Identity=23.3183856502242, Blast_Score=117, Evalue=3e-27,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR022749 - InterPro: IPR003356 - InterPro: IPR002052 - InterPro: IPR002296 - InterPro: IPR004546 [H]
Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]
EC number: =2.1.1.72 [H]
Molecular weight: Translated: 91258; Mature: 91127
Theoretical pI: Translated: 6.48; Mature: 6.48
Prosite motif: PS00092 N6_MTASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 3.0 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAIKKTELYSSLWASCDELRGGMDASQYKDYVLTLLFMKYVSDKYKGDPYGMIVIPKGAS CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC FDDMVALKNDKEIGDKINKIIRRLAEENDLKGVIDEADFNDEDKLGKGKEMMDRLTKLVG CCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH IFEGLDLSGNRADGDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRILAKVIGITPDT HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCC PQDATVYDPTCGSGSLLLKVSDETRRGLSIFGQEMDNATSALARMNMILHNNATAKIWQG CCCCEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC NTLSDPQWKVANGKLKTFDFAVANPPFSNKNWTSGLDLKRDPFERFVWGVPPEKNGDYAF CCCCCCCEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHH LLHIIKSLKSTGKGAVILPHGVLFRGNAEANIRENLIKQGYIKGIIGLPANLFYGTGIPA HHHHHHHHHCCCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCE CIIVIDKEHAHSRKGIFMIDASRGFIKDGNKNRLRSRDIHRIVDVFNHQRTLPGYSRMVP EEEEEECCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCC LSDIAGNDYNLNIPRYIDGGEPEDLHDLSAHLQGGIPARDVDALQDYWRVFPALRDVLFT HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC DDRPGYCRAQVEAQQVKPTILAHQEFKDFSTRSLLPFKAWVKEAALNEIGKGDKPKALIH CCCCCCEEHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH DISEMLLAQYANSELLNKYSVYQILMDYWADTMQDDVYVIMQDGWQAAAHIRELQPVKGK HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCCCC DGKNVWKEAHDFEFDKRRYKADVLPRSLVEIRCFPQQLEALNRAQERSEEASRQLAEFIE CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH EQTGEEDLLAEAKNDKDKVTQKLVNARLTKLKKTTADPDELAALTRCLALIKAEANAKKA HCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHH LKVAQDALDRAVFKQYPTLDEPAIKALVVQDKWLATLQAGIKAEIERITQQLAERVKELE HHHHHHHHHHHHHHHCCCCCCCHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ERYAEPLPALEASVEALSKKVAGHLRAMGLVW HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC >Mature Secondary Structure AIKKTELYSSLWASCDELRGGMDASQYKDYVLTLLFMKYVSDKYKGDPYGMIVIPKGAS CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC FDDMVALKNDKEIGDKINKIIRRLAEENDLKGVIDEADFNDEDKLGKGKEMMDRLTKLVG CCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH IFEGLDLSGNRADGDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRILAKVIGITPDT HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCC PQDATVYDPTCGSGSLLLKVSDETRRGLSIFGQEMDNATSALARMNMILHNNATAKIWQG CCCCEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC NTLSDPQWKVANGKLKTFDFAVANPPFSNKNWTSGLDLKRDPFERFVWGVPPEKNGDYAF CCCCCCCEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHH LLHIIKSLKSTGKGAVILPHGVLFRGNAEANIRENLIKQGYIKGIIGLPANLFYGTGIPA HHHHHHHHHCCCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCE CIIVIDKEHAHSRKGIFMIDASRGFIKDGNKNRLRSRDIHRIVDVFNHQRTLPGYSRMVP EEEEEECCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCC LSDIAGNDYNLNIPRYIDGGEPEDLHDLSAHLQGGIPARDVDALQDYWRVFPALRDVLFT HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC DDRPGYCRAQVEAQQVKPTILAHQEFKDFSTRSLLPFKAWVKEAALNEIGKGDKPKALIH CCCCCCEEHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH DISEMLLAQYANSELLNKYSVYQILMDYWADTMQDDVYVIMQDGWQAAAHIRELQPVKGK HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCCCC DGKNVWKEAHDFEFDKRRYKADVLPRSLVEIRCFPQQLEALNRAQERSEEASRQLAEFIE CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH EQTGEEDLLAEAKNDKDKVTQKLVNARLTKLKKTTADPDELAALTRCLALIKAEANAKKA HCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHH LKVAQDALDRAVFKQYPTLDEPAIKALVVQDKWLATLQAGIKAEIERITQQLAERVKELE HHHHHHHHHHHHHHHCCCCCCCHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ERYAEPLPALEASVEALSKKVAGHLRAMGLVW HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2784505 [H]