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The map label for this gene is pmbA [H]

Identifier: 238921235

GI number: 238921235

Start: 3268039

End: 3269379

Strand: Reverse

Name: pmbA [H]

Synonym: NT01EI_3378

Alternate gene names: 238921235

Gene position: 3269379-3268039 (Counterclockwise)

Preceding gene: 238921236

Following gene: 238921232

Centisome position: 85.76

GC content: 62.86

Gene sequence:

>1341_bases
ATGAAAGTCATCAATCAGGTAGCAGAGCAACGCAAGGCGCTGGAAGAGGCCGTGGCGCAGGCGCTGACGCTGGCGCAGGC
CGGGGCGGATGCGGCAGAAGTGGCGGTGACCCGGACCACCGGCATCAGCGTCAGCACCCGCTTTGGTGAAGTGGAAAACG
TCGAGTTTAACAGCGACGGCGCACTGGGGATCACCGTCTACCATCAGCAGTGTAAAGGCAGTGCCTCCTCTACCGATCTC
AGCCCGCAGGCCATCGCACGTACGGTACAGGCGGCGTTGGACATCGCGCGTTACACCTCACCGGATCCCTGCGCCGGTCC
GGCGTCGCGCGATCTGCTGGCGTTTGAGGCCCCGGATCTGGATCTGTTCCATCCATCTGAGCTGAACGCTGAGCAGGCGA
TTGCGCTGGCCGCAGAGGCCGAGCAGGCGGCCCTGGGTGCCGATGCGCGAATCACCAATACCGAGGGCGGTAGCTTCAAC
AGTCACTATGGCATCCGCGTCTTCGGCAACAGTCACGGCATGTTGCAGAGTTACTGCTCCAGCCGCCATTCTCTGTCCAG
CTGCGTGATTGCCGAGCAGGACGGCGACATGGAGCGCGACTACGCCTATACCATTGCCCGTGATATGGACGATCTGCGTT
CCGCGCGCTGGGTCGGTGAGGAGTGCGCCCGCCGAACGCTGGCGCGTCTGGCGCCGCGTCGTCTACCCACCATGCAGGTG
CCGGTGATGTTCGCCGCGGAGGCCGCTACCGGGCTGTTTGGCCATCTGGTCGGCGCCATTAGTGGCAGCAGCGTGTACCG
CAAGTCTACCTTTTTACTCGATAGCCTGGGCCAGCAGATCCTACCCTCCTGGCTGACCATTGATGAGCAGCCGCACCTGT
TGCGCGCTCTGGCGTCTACCCCCTTTGACAGCGAAGGGGTTCGCACCACACCGCGGCGTATCGTGCAGGACGGCGTACTG
CAGACCTGGCTGCTGACCAGCTATTCAGCGCGTAAGCTGGGGCTGCATAGCACTGGTCACGCAGGTGGCATTCATAACTG
GCGTATCGCCGGCCGTGGGCTGGGATTTGAACAGATGCTGCGCGAGATGGGGCGCGGCTTCCTGGTCACCGAACTGATGG
GGCAGGGGGTGAGCGCGGTGACCGGCGACTATTCGCGCGGCGCATCCGGTTTCTGGGTTGAGAACGGCGAGATCCAGTAT
CCGGTGAGCGAGGTCACCATCGCCGGCAATCTGCGCGATATGTGGGCCAATATGGTTACCATAGGCGACGACATTGAGAC
GCGCAGCAACATTCAGTGTGGCTCGGTGCTGCTGGACAGCATGCAGATCGCCGGGGAGTAA

Upstream 100 bases:

>100_bases
CCCGGGAGATATGGCGCTGTATCACTCAGGCTGCGGCCTGTTGTCATAGCCGACGGCGCGGTGTCGCCCGGTTTAACCGT
TTTGCAATCATGGCGGATCA

Downstream 100 bases:

>100_bases
GGGGTCATATCATCGCCTGACATCAGGGTTTTGCATCGGTGCGGGTAAAAATAAATATCCCCCGGCATAGCCGGGGGTTT
TTCATATGCGCCTATAAAGA

Product: peptidase PmbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKVINQVAEQRKALEEAVAQALTLAQAGADAAEVAVTRTTGISVSTRFGEVENVEFNSDGALGITVYHQQCKGSASSTDL
SPQAIARTVQAALDIARYTSPDPCAGPASRDLLAFEAPDLDLFHPSELNAEQAIALAAEAEQAALGADARITNTEGGSFN
SHYGIRVFGNSHGMLQSYCSSRHSLSSCVIAEQDGDMERDYAYTIARDMDDLRSARWVGEECARRTLARLAPRRLPTMQV
PVMFAAEAATGLFGHLVGAISGSSVYRKSTFLLDSLGQQILPSWLTIDEQPHLLRALASTPFDSEGVRTTPRRIVQDGVL
QTWLLTSYSARKLGLHSTGHAGGIHNWRIAGRGLGFEQMLREMGRGFLVTELMGQGVSAVTGDYSRGASGFWVENGEIQY
PVSEVTIAGNLRDMWANMVTIGDDIETRSNIQCGSVLLDSMQIAGE

Sequences:

>Translated_446_residues
MKVINQVAEQRKALEEAVAQALTLAQAGADAAEVAVTRTTGISVSTRFGEVENVEFNSDGALGITVYHQQCKGSASSTDL
SPQAIARTVQAALDIARYTSPDPCAGPASRDLLAFEAPDLDLFHPSELNAEQAIALAAEAEQAALGADARITNTEGGSFN
SHYGIRVFGNSHGMLQSYCSSRHSLSSCVIAEQDGDMERDYAYTIARDMDDLRSARWVGEECARRTLARLAPRRLPTMQV
PVMFAAEAATGLFGHLVGAISGSSVYRKSTFLLDSLGQQILPSWLTIDEQPHLLRALASTPFDSEGVRTTPRRIVQDGVL
QTWLLTSYSARKLGLHSTGHAGGIHNWRIAGRGLGFEQMLREMGRGFLVTELMGQGVSAVTGDYSRGASGFWVENGEIQY
PVSEVTIAGNLRDMWANMVTIGDDIETRSNIQCGSVLLDSMQIAGE
>Mature_446_residues
MKVINQVAEQRKALEEAVAQALTLAQAGADAAEVAVTRTTGISVSTRFGEVENVEFNSDGALGITVYHQQCKGSASSTDL
SPQAIARTVQAALDIARYTSPDPCAGPASRDLLAFEAPDLDLFHPSELNAEQAIALAAEAEQAALGADARITNTEGGSFN
SHYGIRVFGNSHGMLQSYCSSRHSLSSCVIAEQDGDMERDYAYTIARDMDDLRSARWVGEECARRTLARLAPRRLPTMQV
PVMFAAEAATGLFGHLVGAISGSSVYRKSTFLLDSLGQQILPSWLTIDEQPHLLRALASTPFDSEGVRTTPRRIVQDGVL
QTWLLTSYSARKLGLHSTGHAGGIHNWRIAGRGLGFEQMLREMGRGFLVTELMGQGVSAVTGDYSRGASGFWVENGEIQY
PVSEVTIAGNLRDMWANMVTIGDDIETRSNIQCGSVLLDSMQIAGE

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=446, Percent_Identity=79.5964125560538, Blast_Score=755, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48003; Mature: 48003

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVINQVAEQRKALEEAVAQALTLAQAGADAAEVAVTRTTGISVSTRFGEVENVEFNSDG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCEEEECCCCCCCEEECCCC
ALGITVYHQQCKGSASSTDLSPQAIARTVQAALDIARYTSPDPCAGPASRDLLAFEAPDL
CEEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
DLFHPSELNAEQAIALAAEAEQAALGADARITNTEGGSFNSHYGIRVFGNSHGMLQSYCS
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEECCCCHHHHHHH
SRHSLSSCVIAEQDGDMERDYAYTIARDMDDLRSARWVGEECARRTLARLAPRRLPTMQV
HHHCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
PVMFAAEAATGLFGHLVGAISGSSVYRKSTFLLDSLGQQILPSWLTIDEQPHLLRALAST
CCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHCC
PFDSEGVRTTPRRIVQDGVLQTWLLTSYSARKLGLHSTGHAGGIHNWRIAGRGLGFEQML
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCEEEECCCCCHHHHH
REMGRGFLVTELMGQGVSAVTGDYSRGASGFWVENGEIQYPVSEVTIAGNLRDMWANMVT
HHHCCCEEEHHHHCCCCCCCCCCCCCCCCCEEEECCEEECCHHHEEEECCHHHHHHHHEE
IGDDIETRSNIQCGSVLLDSMQIAGE
ECCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVINQVAEQRKALEEAVAQALTLAQAGADAAEVAVTRTTGISVSTRFGEVENVEFNSDG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCEEEECCCCCCCEEECCCC
ALGITVYHQQCKGSASSTDLSPQAIARTVQAALDIARYTSPDPCAGPASRDLLAFEAPDL
CEEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
DLFHPSELNAEQAIALAAEAEQAALGADARITNTEGGSFNSHYGIRVFGNSHGMLQSYCS
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEECCCCHHHHHHH
SRHSLSSCVIAEQDGDMERDYAYTIARDMDDLRSARWVGEECARRTLARLAPRRLPTMQV
HHHCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
PVMFAAEAATGLFGHLVGAISGSSVYRKSTFLLDSLGQQILPSWLTIDEQPHLLRALAST
CCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHCC
PFDSEGVRTTPRRIVQDGVLQTWLLTSYSARKLGLHSTGHAGGIHNWRIAGRGLGFEQML
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCEEEECCCCCHHHHH
REMGRGFLVTELMGQGVSAVTGDYSRGASGFWVENGEIQYPVSEVTIAGNLRDMWANMVT
HHHCCCEEEHHHHCCCCCCCCCCCCCCCCCEEEECCEEECCHHHEEEECCHHHHHHHHEE
IGDDIETRSNIQCGSVLLDSMQIAGE
ECCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]