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The map label for this gene is mscS

Identifier: 238921223

GI number: 238921223

Start: 3251666

End: 3252526

Strand: Reverse

Name: mscS

Synonym: NT01EI_3366

Alternate gene names: 238921223

Gene position: 3252526-3251666 (Counterclockwise)

Preceding gene: 238921224

Following gene: 238921222

Centisome position: 85.32

GC content: 58.65

Gene sequence:

>861_bases
ATGAAAGAATTGGATGTAGTTGACGGCATACAGAGCGCCGGCGGTTGGATCGTCCGTAACCAGGATTTGCTGCTGGGCTA
TGCCGTCAATCTGGTGGCGGCGGTAGTGATCCTGATCATCGGTTCCCTGATCGCCCGCGGCATTTCGACGACGCTGATTC
GCCTGCTGAAGGCGCGTGGTCTGGATGTGACGGTAGTGCATTTCCTGGCGGCGATGGTACGTTATGCCATTCTGGCCTTT
ACCATTATCGCCGCGCTGGGGCGGCTTGGGGTGCAGACCACCTCGGTGATCGCTGTGCTGGGTGCCGCCGGTTTGGCGGT
CGGTCTGGCGCTACAGGGGTCGTTGTCCAACTTTGCCGCCGGCGTGCTGCTGGTGCTGTTCCGCCCGTTCCGCGCGGGAG
AGGTCGTGGATCTGGGCGGCGTCACCGGCACGGTACGCGAGGTGCAGATTTTCTCTACCACGCTGGCCACAGCTGACAAT
AAAGTGATTGTGGTGCCCAATGGCAAGATCATTGCCGGCAACATCATCAACTTCTCCCGCGAGCCGAAGCGCCGGATCGA
TATTATCGTGGGTGTGGCCTACGACGCCGATATCGACGTGGTCAAGCGGGTGCTGGGCGATGTGGTGGCGGCCGATACGC
GCATTCTGCACGATGATGGCGTTACCATTCGCCTGAACGAAATGGCGGCCTCGTCGCTCAATTTTGTGGTGCGTGTCTGG
GGCAACAACGCCGACTATTGGGCCATCTATTTTGATCTGATGGAGAATTTCAAACGCGCGCTGGATGCCAATAATATTGG
CATTCCGTTCCCACAGATGGACGTGCATCTGTATCAGGCTGTCAAAGCCCGTGCCGAGTGA

Upstream 100 bases:

>100_bases
CCCTTATAATCATTGGGCTGCGATCTGCGGCCTGTTTTGCCCGTGTTATCGGGGTTACGGTGACGGAGTCAGGTAATGAA
ATCAGAGGGTAAAAAATAAG

Downstream 100 bases:

>100_bases
TCTCCTCCCGTCTGGCCGACGCCCCGGCCAGACACCATGAGCCCGTCTAATCTGCGATAAGAAATCGCTATTTCCCCCGT
CGCTGCCCGGCTGTTTATGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKELDVVDGIQSAGGWIVRNQDLLLGYAVNLVAAVVILIIGSLIARGISTTLIRLLKARGLDVTVVHFLAAMVRYAILAF
TIIAALGRLGVQTTSVIAVLGAAGLAVGLALQGSLSNFAAGVLLVLFRPFRAGEVVDLGGVTGTVREVQIFSTTLATADN
KVIVVPNGKIIAGNIINFSREPKRRIDIIVGVAYDADIDVVKRVLGDVVAADTRILHDDGVTIRLNEMAASSLNFVVRVW
GNNADYWAIYFDLMENFKRALDANNIGIPFPQMDVHLYQAVKARAE

Sequences:

>Translated_286_residues
MKELDVVDGIQSAGGWIVRNQDLLLGYAVNLVAAVVILIIGSLIARGISTTLIRLLKARGLDVTVVHFLAAMVRYAILAF
TIIAALGRLGVQTTSVIAVLGAAGLAVGLALQGSLSNFAAGVLLVLFRPFRAGEVVDLGGVTGTVREVQIFSTTLATADN
KVIVVPNGKIIAGNIINFSREPKRRIDIIVGVAYDADIDVVKRVLGDVVAADTRILHDDGVTIRLNEMAASSLNFVVRVW
GNNADYWAIYFDLMENFKRALDANNIGIPFPQMDVHLYQAVKARAE
>Mature_286_residues
MKELDVVDGIQSAGGWIVRNQDLLLGYAVNLVAAVVILIIGSLIARGISTTLIRLLKARGLDVTVVHFLAAMVRYAILAF
TIIAALGRLGVQTTSVIAVLGAAGLAVGLALQGSLSNFAAGVLLVLFRPFRAGEVVDLGGVTGTVREVQIFSTTLATADN
KVIVVPNGKIIAGNIINFSREPKRRIDIIVGVAYDADIDVVKRVLGDVVAADTRILHDDGVTIRLNEMAASSLNFVVRVW
GNNADYWAIYFDLMENFKRALDANNIGIPFPQMDVHLYQAVKARAE

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family

Homologues:

Organism=Escherichia coli, GI1789291, Length=285, Percent_Identity=64.2105263157895, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1786670, Length=212, Percent_Identity=27.8301886792453, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787591, Length=254, Percent_Identity=24.8031496062992, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI2367355, Length=251, Percent_Identity=20.7171314741036, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCS_EDWI9 (O52401)

Other databases:

- EMBL:   AF037440
- EMBL:   CP001600
- RefSeq:   YP_002934738.1
- ProteinModelPortal:   O52401
- SMR:   O52401
- GeneID:   7959665
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3366
- OMA:   LESPAPF
- ProtClustDB:   CLSK2505906
- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix; SSF82689 MscC_channel_C; SSF82861 MscS_transmembr; SSF50182 Sm_like_riboprot

EC number: NA

Molecular weight: Translated: 30648; Mature: 30648

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1f28d7a4)-; HASH(0x1fab3ae4)-; HASH(0x1fc17ec4)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELDVVDGIQSAGGWIVRNQDLLLGYAVNLVAAVVILIIGSLIARGISTTLIRLLKARG
CCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDVTVVHFLAAMVRYAILAFTIIAALGRLGVQTTSVIAVLGAAGLAVGLALQGSLSNFAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GVLLVLFRPFRAGEVVDLGGVTGTVREVQIFSTTLATADNKVIVVPNGKIIAGNIINFSR
HHHHHHHCCCCCCCEEEECCCCCCHHHEEEEHHHHHCCCCEEEEEECCEEEEEEEECCCC
EPKRRIDIIVGVAYDADIDVVKRVLGDVVAADTRILHDDGVTIRLNEMAASSLNFVVRVW
CCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCEEEEHHHHHCCCEEEEEEE
GNNADYWAIYFDLMENFKRALDANNIGIPFPQMDVHLYQAVKARAE
CCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCH
>Mature Secondary Structure
MKELDVVDGIQSAGGWIVRNQDLLLGYAVNLVAAVVILIIGSLIARGISTTLIRLLKARG
CCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDVTVVHFLAAMVRYAILAFTIIAALGRLGVQTTSVIAVLGAAGLAVGLALQGSLSNFAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GVLLVLFRPFRAGEVVDLGGVTGTVREVQIFSTTLATADNKVIVVPNGKIIAGNIINFSR
HHHHHHHCCCCCCCEEEECCCCCCHHHEEEEHHHHHCCCCEEEEEECCEEEEEEEECCCC
EPKRRIDIIVGVAYDADIDVVKRVLGDVVAADTRILHDDGVTIRLNEMAASSLNFVVRVW
CCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCEEEEHHHHHCCCEEEEEEE
GNNADYWAIYFDLMENFKRALDANNIGIPFPQMDVHLYQAVKARAE
CCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086