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The map label for this gene is fba

Identifier: 238921224

GI number: 238921224

Start: 3252801

End: 3253877

Strand: Reverse

Name: fba

Synonym: NT01EI_3367

Alternate gene names: 238921224

Gene position: 3253877-3252801 (Counterclockwise)

Preceding gene: 238921225

Following gene: 238921223

Centisome position: 85.35

GC content: 57.2

Gene sequence:

>1077_bases
ATGTCTAAAATCTTTGACTTTGTTAAACCGGGTGTCATCTTTGGCGATGACGTTCAGAAAGTATTCCAGGTTGCGAAAGA
GAACAAATTCGCTCTGCCGGCCGTTAACTGCGTCGGTACGGACTCCATCAACGCGGTGATGGAGGCTGCAGCCAAGGTAC
GCGCGCCGATCATCGTTCAGTTCTCCAACGGCGGTGCTGCCTTCATCGCCGGTAAAGGACTGAAGCTGGAGGGCCAGCAG
GGGGCCATCCTCGGCGCGATCGCCGGTGCGCACCACGTGCATCAGATGGCCGAATACTACGGTGTTCCGGTGATCCTGCA
TACCGACCACTGCGCCAAGAAGCTGCTGCCGTGGCTGGACGGGCTGCTGGATGCCGGTGAGAAGCACTTCGCCGCGACGG
GCAAGCCGCTGTTCTCCTCCCACATGATCGATCTTTCCGAAGAGTCTCTGGAAGAGAACATTGAAATCTGCTCTCAGTAC
CTGGCCCGCATGTCCAAGATCGGTATGACCCTGGAGCTGGAGCTGGGTTGCACCGGTGGTGAAGAAGACGGTGTGGACAA
CAGTCACCTGGACAACTCCGCGCTGTACACCCAGCCGGAGGACGTGGACTACGCGTTCACCAAGCTGAGCGCCATCAGCC
CGCGTTTCACCATCGCTGCCTCCTTCGGCAACGTGCACGGCGTGTACAAGCCGGGTAACGTGCAGCTGACGCCGGTGATC
CTGAAAAACTCTCAGGAGTATGTCTCCAAGAAGCATAACCTGCCGCATAACAGCCTGAACTTTGTCTTCCACGGTGGCTC
CGGCTCTACCGCGGCAGAGATCAAAGAAGCGGTCAGCTACGGTGTGGTGAAGATGAACATCGACACCGATACCCAGTGGG
CAACCTGGGAAGGTGTGCTGAAGTACTACAAGAAGAACGAAGGTTATCTGCAGGGCCAGCTGGGTAATCCGGAAGGCGAC
GACAAGCCGAACAAGAAGTACTACGATCCACGCGTATGGCTGCGTGCCGCTCAGACTGGCATGATCGAGCGTCTGGAGCA
GGCGTTCAAAGAGCTGAACTGTATCGACGTGCTGTAA

Upstream 100 bases:

>100_bases
ACGGGAGGCCATCATGCCTCCCGTTGTTTTATCGCGCCTTGGCGCGGTAATGTTGATCCGATTGGAAAACACTCACGCCC
ACGAAACAGGACGATTAGAC

Downstream 100 bases:

>100_bases
TCGTCGTCAATCGGCTGGAGAAACCCCGCGTTGCGGGGTTTTTTTATGCCGGGAGATCGCCGGGAAAGGGAAATATCATC
GCGAGCGGCCATATCAATAC

Product: fructose-bisphosphate aldolase

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MSKIFDFVKPGVIFGDDVQKVFQVAKENKFALPAVNCVGTDSINAVMEAAAKVRAPIIVQFSNGGAAFIAGKGLKLEGQQ
GAILGAIAGAHHVHQMAEYYGVPVILHTDHCAKKLLPWLDGLLDAGEKHFAATGKPLFSSHMIDLSEESLEENIEICSQY
LARMSKIGMTLELELGCTGGEEDGVDNSHLDNSALYTQPEDVDYAFTKLSAISPRFTIAASFGNVHGVYKPGNVQLTPVI
LKNSQEYVSKKHNLPHNSLNFVFHGGSGSTAAEIKEAVSYGVVKMNIDTDTQWATWEGVLKYYKKNEGYLQGQLGNPEGD
DKPNKKYYDPRVWLRAAQTGMIERLEQAFKELNCIDVL

Sequences:

>Translated_358_residues
MSKIFDFVKPGVIFGDDVQKVFQVAKENKFALPAVNCVGTDSINAVMEAAAKVRAPIIVQFSNGGAAFIAGKGLKLEGQQ
GAILGAIAGAHHVHQMAEYYGVPVILHTDHCAKKLLPWLDGLLDAGEKHFAATGKPLFSSHMIDLSEESLEENIEICSQY
LARMSKIGMTLELELGCTGGEEDGVDNSHLDNSALYTQPEDVDYAFTKLSAISPRFTIAASFGNVHGVYKPGNVQLTPVI
LKNSQEYVSKKHNLPHNSLNFVFHGGSGSTAAEIKEAVSYGVVKMNIDTDTQWATWEGVLKYYKKNEGYLQGQLGNPEGD
DKPNKKYYDPRVWLRAAQTGMIERLEQAFKELNCIDVL
>Mature_357_residues
SKIFDFVKPGVIFGDDVQKVFQVAKENKFALPAVNCVGTDSINAVMEAAAKVRAPIIVQFSNGGAAFIAGKGLKLEGQQG
AILGAIAGAHHVHQMAEYYGVPVILHTDHCAKKLLPWLDGLLDAGEKHFAATGKPLFSSHMIDLSEESLEENIEICSQYL
ARMSKIGMTLELELGCTGGEEDGVDNSHLDNSALYTQPEDVDYAFTKLSAISPRFTIAASFGNVHGVYKPGNVQLTPVIL
KNSQEYVSKKHNLPHNSLNFVFHGGSGSTAAEIKEAVSYGVVKMNIDTDTQWATWEGVLKYYKKNEGYLQGQLGNPEGDD
KPNKKYYDPRVWLRAAQTGMIERLEQAFKELNCIDVL

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family

Homologues:

Organism=Escherichia coli, GI1789293, Length=359, Percent_Identity=87.7437325905292, Blast_Score=665, Evalue=0.0,
Organism=Escherichia coli, GI87082041, Length=336, Percent_Identity=24.1071428571429, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788072, Length=272, Percent_Identity=26.4705882352941, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322790, Length=351, Percent_Identity=49.002849002849, Blast_Score=359, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALF_EDWI9 (O52402)

Other databases:

- EMBL:   AF037440
- EMBL:   CP001600
- RefSeq:   YP_002934739.1
- GeneID:   7959666
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3367
- OMA:   QAYCAEK
- ProtClustDB:   PRK09197
- BRENDA:   4.1.2.13
- GO:   GO:0006096
- InterPro:   IPR013785
- InterPro:   IPR006411
- InterPro:   IPR000771
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF001359
- TIGRFAMs:   TIGR00167
- TIGRFAMs:   TIGR01520

Pfam domain/function: PF01116 F_bP_aldolase

EC number: =4.1.2.13

Molecular weight: Translated: 39155; Mature: 39024

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00602 ALDOLASE_CLASS_II_1; PS00806 ALDOLASE_CLASS_II_2

Important sites: ACT_SITE 109-109 BINDING 62-62 BINDING 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIFDFVKPGVIFGDDVQKVFQVAKENKFALPAVNCVGTDSINAVMEAAAKVRAPIIVQ
CCCHHHHHCCCCEECHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEE
FSNGGAAFIAGKGLKLEGQQGAILGAIAGAHHVHQMAEYYGVPVILHTDHCAKKLLPWLD
ECCCCEEEEECCCEEEECCCCCEEEHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHH
GLLDAGEKHFAATGKPLFSSHMIDLSEESLEENIEICSQYLARMSKIGMTLELELGCTGG
HHHCCCCCCEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
EEDGVDNSHLDNSALYTQPEDVDYAFTKLSAISPRFTIAASFGNVHGVYKPGNVQLTPVI
CCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEECCCCEEEEEEE
LKNSQEYVSKKHNLPHNSLNFVFHGGSGSTAAEIKEAVSYGVVKMNIDTDTQWATWEGVL
ECCCHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHH
KYYKKNEGYLQGQLGNPEGDDKPNKKYYDPRVWLRAAQTGMIERLEQAFKELNCIDVL
HHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure 
SKIFDFVKPGVIFGDDVQKVFQVAKENKFALPAVNCVGTDSINAVMEAAAKVRAPIIVQ
CCHHHHHCCCCEECHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEE
FSNGGAAFIAGKGLKLEGQQGAILGAIAGAHHVHQMAEYYGVPVILHTDHCAKKLLPWLD
ECCCCEEEEECCCEEEECCCCCEEEHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHH
GLLDAGEKHFAATGKPLFSSHMIDLSEESLEENIEICSQYLARMSKIGMTLELELGCTGG
HHHCCCCCCEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
EEDGVDNSHLDNSALYTQPEDVDYAFTKLSAISPRFTIAASFGNVHGVYKPGNVQLTPVI
CCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEECCCCEEEEEEE
LKNSQEYVSKKHNLPHNSLNFVFHGGSGSTAAEIKEAVSYGVVKMNIDTDTQWATWEGVL
ECCCHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHH
KYYKKNEGYLQGQLGNPEGDDKPNKKYYDPRVWLRAAQTGMIERLEQAFKELNCIDVL
HHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12542086