Click here to switch to the map view.

The map label for this gene is gcvT

Identifier: 238921211

GI number: 238921211

Start: 3239469

End: 3240593

Strand: Reverse

Name: gcvT

Synonym: NT01EI_3353

Alternate gene names: 238921211

Gene position: 3240593-3239469 (Counterclockwise)

Preceding gene: 238921212

Following gene: 238921210

Centisome position: 85.0

GC content: 63.56

Gene sequence:

>1125_bases
ATGACAAAACAGACGGTGCTTTATGATCAGCATGTGGCCGAAGGGGCGCGCATGGTGGATTTCCATGGCTGGATGATGCC
GCTGCACTATGGCTCCCAGCTGGAGGAGCACCATGCGGTGCGCAGCGACGCCGGTATGTTTGATGTTTCCCATATGACCA
TCGTGGATCTGCATGGCGTCAGGGTGCGGGACTTTCTGCGCCATTTGTTGGCCAACGATGTGGCACGCCTGACGCAGCCG
GGTAAGGCGCTGTACAGCGCAATGCTGAACGCCAGCGGCGGGGTGATCGACGATCTGATCGTCTACTTTATGGCGGATCA
TCATTTTCGGCTGGTAGTTAACTCGGCGACCCGTGAGCGGGATTTAGCCTGGATCGGCGAACATGCGCCCGCCTTTGGCG
TGGAGATATGCGAGCGTCGCGACCTGGCGCTGATTGCGGTACAGGGGCCAACGGCCCGCCAGCGGGTAGATGCGCTGCTG
ACGCCGGCGCAGCGTCAGATGGTCGCCGGGATGAAACCCTTCTTTGGCCGTCAGGTGGGATCGCTGTTTATCGCCACCAC
CGGCTACACCGGGGAGGATGGCTATGAAATAGCCCTGCCGCAGGATGAGGCGGTGGCCTTCTGGCAGCAGCTGGTGCAGG
CGGGTATCAGGCCCTGTGGGCTGGCCGCCCGTGACACACTGCGCCTGGAGGCAGGAATGAACCTGTATGGTCAGGAGATG
GATGAGCAGATCTCGCCGCTGGCGGCCAACATGGGCTGGACCATCGCCTGGGCGCCGCCGGAGCGTGATTTTATTGGCCG
GGCGGCGCTGGAGCGTCAGCAGAGCCAGCATCCCGAGCAGCTGGTCGGTCTGGTGATGCGGGAGAAGGGGGTGCTGCGGG
CGGGCATGACGATCCGTTGCCGCGATGGGCAGGGAGACCCTTGCCTGGGGACGATCACCAGCGGATCGTTTTCACCGACG
CTGGGGTGCAGCATTGCGCTGGCGCGGGTACCGCAGGGGATCGGGGGTGAGGCGTGGGTCGAGATCCGCGGTCGTGAGCT
GGCGCTCAGCGTGGTGAAACCGGGCTTTGTCCGTCACGGCCACAGCCTGATCCCACCCGCCGATGCGACGGCAGTAGGCA
ACTAA

Upstream 100 bases:

>100_bases
GTGATACTGTCGGGTTGAGAGATATCGATTGCGCGTCGTGGAGGGGCCACGGCGGCGTGGGCGCGGTGGTGTACGGCCGC
ATACTCAGTCGGGGACGATA

Downstream 100 bases:

>100_bases
CCAAGTGAAGCAACACATCATTCCAGGAGAGGGTGGCGATGAGTAATGTGCCGACAGAGTTGAAGTATACCGCGTCCCAT
GAGTGGGTACGCGCAGAGGA

Product: glycine cleavage system T protein

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MTKQTVLYDQHVAEGARMVDFHGWMMPLHYGSQLEEHHAVRSDAGMFDVSHMTIVDLHGVRVRDFLRHLLANDVARLTQP
GKALYSAMLNASGGVIDDLIVYFMADHHFRLVVNSATRERDLAWIGEHAPAFGVEICERRDLALIAVQGPTARQRVDALL
TPAQRQMVAGMKPFFGRQVGSLFIATTGYTGEDGYEIALPQDEAVAFWQQLVQAGIRPCGLAARDTLRLEAGMNLYGQEM
DEQISPLAANMGWTIAWAPPERDFIGRAALERQQSQHPEQLVGLVMREKGVLRAGMTIRCRDGQGDPCLGTITSGSFSPT
LGCSIALARVPQGIGGEAWVEIRGRELALSVVKPGFVRHGHSLIPPADATAVGN

Sequences:

>Translated_374_residues
MTKQTVLYDQHVAEGARMVDFHGWMMPLHYGSQLEEHHAVRSDAGMFDVSHMTIVDLHGVRVRDFLRHLLANDVARLTQP
GKALYSAMLNASGGVIDDLIVYFMADHHFRLVVNSATRERDLAWIGEHAPAFGVEICERRDLALIAVQGPTARQRVDALL
TPAQRQMVAGMKPFFGRQVGSLFIATTGYTGEDGYEIALPQDEAVAFWQQLVQAGIRPCGLAARDTLRLEAGMNLYGQEM
DEQISPLAANMGWTIAWAPPERDFIGRAALERQQSQHPEQLVGLVMREKGVLRAGMTIRCRDGQGDPCLGTITSGSFSPT
LGCSIALARVPQGIGGEAWVEIRGRELALSVVKPGFVRHGHSLIPPADATAVGN
>Mature_373_residues
TKQTVLYDQHVAEGARMVDFHGWMMPLHYGSQLEEHHAVRSDAGMFDVSHMTIVDLHGVRVRDFLRHLLANDVARLTQPG
KALYSAMLNASGGVIDDLIVYFMADHHFRLVVNSATRERDLAWIGEHAPAFGVEICERRDLALIAVQGPTARQRVDALLT
PAQRQMVAGMKPFFGRQVGSLFIATTGYTGEDGYEIALPQDEAVAFWQQLVQAGIRPCGLAARDTLRLEAGMNLYGQEMD
EQISPLAANMGWTIAWAPPERDFIGRAALERQQSQHPEQLVGLVMREKGVLRAGMTIRCRDGQGDPCLGTITSGSFSPTL
GCSIALARVPQGIGGEAWVEIRGRELALSVVKPGFVRHGHSLIPPADATAVGN

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=375, Percent_Identity=33.8666666666667, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI257796258, Length=346, Percent_Identity=34.393063583815, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI257796254, Length=365, Percent_Identity=31.2328767123288, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI257796256, Length=312, Percent_Identity=33.0128205128205, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI24797151, Length=348, Percent_Identity=27.5862068965517, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI197927446, Length=365, Percent_Identity=26.027397260274, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI21361378, Length=365, Percent_Identity=26.027397260274, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1789272, Length=363, Percent_Identity=70.2479338842975, Blast_Score=530, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17560118, Length=373, Percent_Identity=32.9758713136729, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71994045, Length=369, Percent_Identity=24.1192411924119, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994052, Length=332, Percent_Identity=24.3975903614458, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320222, Length=378, Percent_Identity=35.4497354497354, Blast_Score=189, Evalue=9e-49,
Organism=Drosophila melanogaster, GI20129441, Length=379, Percent_Identity=30.3430079155673, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI28571104, Length=255, Percent_Identity=28.2352941176471, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI20130091, Length=362, Percent_Identity=25.6906077348066, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_EDWI9 (C5BAT2)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934726.1
- ProteinModelPortal:   C5BAT2
- GeneID:   7959653
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3353
- OMA:   KALYGGM
- ProtClustDB:   CLSK2813618
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 40938; Mature: 40807

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQTVLYDQHVAEGARMVDFHGWMMPLHYGSQLEEHHAVRSDAGMFDVSHMTIVDLHGV
CCCCEEEEHHHHHCCCEEEEECCEEEEHHCCCHHHHHHHHHCCCCEEEECCEEEEEECCC
RVRDFLRHLLANDVARLTQPGKALYSAMLNASGGVIDDLIVYFMADHHFRLVVNSATRER
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCC
DLAWIGEHAPAFGVEICERRDLALIAVQGPTARQRVDALLTPAQRQMVAGMKPFFGRQVG
CHHEECCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCHHHHHHHHCCCCHHHHHHC
SLFIATTGYTGEDGYEIALPQDEAVAFWQQLVQAGIRPCGLAARDTLRLEAGMNLYGQEM
CEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHEEEECCCCHHHHHH
DEQISPLAANMGWTIAWAPPERDFIGRAALERQQSQHPEQLVGLVMREKGVLRAGMTIRC
HHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEECCCEEEE
RDGQGDPCLGTITSGSFSPTLGCSIALARVPQGIGGEAWVEIRGRELALSVVKPGFVRHG
ECCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCEEEEEECCCCHHHCC
HSLIPPADATAVGN
CCCCCCCCCCCCCC
>Mature Secondary Structure 
TKQTVLYDQHVAEGARMVDFHGWMMPLHYGSQLEEHHAVRSDAGMFDVSHMTIVDLHGV
CCCEEEEHHHHHCCCEEEEECCEEEEHHCCCHHHHHHHHHCCCCEEEECCEEEEEECCC
RVRDFLRHLLANDVARLTQPGKALYSAMLNASGGVIDDLIVYFMADHHFRLVVNSATRER
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCC
DLAWIGEHAPAFGVEICERRDLALIAVQGPTARQRVDALLTPAQRQMVAGMKPFFGRQVG
CHHEECCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCHHHHHHHHCCCCHHHHHHC
SLFIATTGYTGEDGYEIALPQDEAVAFWQQLVQAGIRPCGLAARDTLRLEAGMNLYGQEM
CEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHEEEECCCCHHHHHH
DEQISPLAANMGWTIAWAPPERDFIGRAALERQQSQHPEQLVGLVMREKGVLRAGMTIRC
HHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEECCCEEEE
RDGQGDPCLGTITSGSFSPTLGCSIALARVPQGIGGEAWVEIRGRELALSVVKPGFVRHG
ECCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCEEEEEECCCCHHHCC
HSLIPPADATAVGN
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA