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The map label for this gene is gcvH

Identifier: 238921210

GI number: 238921210

Start: 3239044

End: 3239430

Strand: Reverse

Name: gcvH

Synonym: NT01EI_3352

Alternate gene names: 238921210

Gene position: 3239430-3239044 (Counterclockwise)

Preceding gene: 238921211

Following gene: 238921209

Centisome position: 84.97

GC content: 59.95

Gene sequence:

>387_bases
ATGAGTAATGTGCCGACAGAGTTGAAGTATACCGCGTCCCATGAGTGGGTACGCGCAGAGGAAAACGGGGTATTTTGCGT
TGGGATCACCGACCATGCCCAGAGTTTGCTGGGCGATATGGTGTTTGTGGATTTGCCTGAGGTGGGGGCGCATATCGACA
CCGGTGCGGAGTGCGCCGTGGCTGAGTCGGTCAAGGCGGCATCGGATATCTATAGCCCGCTGGCGGGAGAGATCGTGGCC
ATAAACGGTTCTCTGGAGGAGGCGCCGGAACAGGTCAATGCGGCGCCCTATGATGAGGGCTGGCTGTTCCGCATCCGGGC
TGACGATCCTGCCGAGTTTGCGGCGCTGCTCAGCGCCGAAGGGTATCTGGCCGCGCTGGACGAATAA

Upstream 100 bases:

>100_bases
GCTTTGTCCGTCACGGCCACAGCCTGATCCCACCCGCCGATGCGACGGCAGTAGGCAACTAACCAAGTGAAGCAACACAT
CATTCCAGGAGAGGGTGGCG

Downstream 100 bases:

>100_bases
GCGCGTGACCGCCGGGCCGCCTCAGTGGCGGCCTTTTGGTATGATTTGCCGGGCGCCGGGCTGCGGCGCAATCCAGGAAT
GCGTAAGCTATGACTCAGAC

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 127

Protein sequence:

>128_residues
MSNVPTELKYTASHEWVRAEENGVFCVGITDHAQSLLGDMVFVDLPEVGAHIDTGAECAVAESVKAASDIYSPLAGEIVA
INGSLEEAPEQVNAAPYDEGWLFRIRADDPAEFAALLSAEGYLAALDE

Sequences:

>Translated_128_residues
MSNVPTELKYTASHEWVRAEENGVFCVGITDHAQSLLGDMVFVDLPEVGAHIDTGAECAVAESVKAASDIYSPLAGEIVA
INGSLEEAPEQVNAAPYDEGWLFRIRADDPAEFAALLSAEGYLAALDE
>Mature_127_residues
SNVPTELKYTASHEWVRAEENGVFCVGITDHAQSLLGDMVFVDLPEVGAHIDTGAECAVAESVKAASDIYSPLAGEIVAI
NGSLEEAPEQVNAAPYDEGWLFRIRADDPAEFAALLSAEGYLAALDE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=114, Percent_Identity=47.3684210526316, Blast_Score=111, Evalue=2e-25,
Organism=Homo sapiens, GI89057342, Length=114, Percent_Identity=46.4912280701754, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789271, Length=128, Percent_Identity=61.71875, Blast_Score=170, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17507493, Length=113, Percent_Identity=46.0176991150442, Blast_Score=109, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17551294, Length=113, Percent_Identity=44.2477876106195, Blast_Score=106, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319272, Length=128, Percent_Identity=39.0625, Blast_Score=96, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=42.5, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_EDWI9 (C5BAT1)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934725.1
- GeneID:   7959652
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3352
- OMA:   MSELRFT
- ProtClustDB:   CLSK2813617
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13626; Mature: 13495

Theoretical pI: Translated: 3.75; Mature: 3.75

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVPTELKYTASHEWVRAEENGVFCVGITDHAQSLLGDMVFVDLPEVGAHIDTGAECAV
CCCCCCCEEEECCCCEEEECCCCEEEEEECHHHHHHHHCEEEEECCCCCCCCCCCCCHHH
AESVKAASDIYSPLAGEIVAINGSLEEAPEQVNAAPYDEGWLFRIRADDPAEFAALLSAE
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHCCC
GYLAALDE
CEEEEECC
>Mature Secondary Structure 
SNVPTELKYTASHEWVRAEENGVFCVGITDHAQSLLGDMVFVDLPEVGAHIDTGAECAV
CCCCCCEEEECCCCEEEECCCCEEEEEECHHHHHHHHCEEEEECCCCCCCCCCCCCHHH
AESVKAASDIYSPLAGEIVAINGSLEEAPEQVNAAPYDEGWLFRIRADDPAEFAALLSAE
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHCCC
GYLAALDE
CEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA