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The map label for this gene is hybF [H]

Identifier: 238921141

GI number: 238921141

Start: 3162043

End: 3162384

Strand: Direct

Name: hybF [H]

Synonym: NT01EI_3282

Alternate gene names: 238921141

Gene position: 3162043-3162384 (Clockwise)

Preceding gene: 238921140

Following gene: 238921142

Centisome position: 82.94

GC content: 59.36

Gene sequence:

>342_bases
ATGCATGAATTATCCCTTTGCCAAAGCGCACTTGACATCATTGAGCGCCAGGCGCGCGACAGCGGCGCCAAGCGCGTCAC
GTCGGTCTGGCTGGACATTGGCGCCCTCTCCTGCATTGAAGAGAGCGCATTAACCTTCTGTTTTGAGATCGTCTGTCGCG
GCACCCTGGCCGAAGGCTGCGCGCTGCGGATCAACACCGTTCCAGCCCAGGCATGGTGCTGGAACTGCAGCCGCCCGATC
ACGGTAACCAGCCACGATGCCGGCTGCCCGCACTGCCAGAGCCACAATCTGCGCGTCGATGGCGGTGACAGCATGCAAAT
CAAACAGATTGAGATTGAGTAA

Upstream 100 bases:

>100_bases
TCATGCAGCGCCGAGGAATCGGTGAACTGATGACGGTAATCAAGGTTCAGCCCTCCGCTTTCCCCGAGGATAAGCCCGGT
ACAACGAAAGAAGAATCACC

Downstream 100 bases:

>100_bases
CCCTAACGCTCAGGAGATCCCCTATATGTGTACCACCTGCGGCTGTGCAGCCGGCGAACTGCATATTGAAGGCGACGAAC
ACCATCACGCCCACCGTCAC

Product: hydrogenase nickel insertion protein HypA

Products: NA

Alternate protein names: Hydrogenase-2 operon protein hybF [H]

Number of amino acids: Translated: 113; Mature: 113

Protein sequence:

>113_residues
MHELSLCQSALDIIERQARDSGAKRVTSVWLDIGALSCIEESALTFCFEIVCRGTLAEGCALRINTVPAQAWCWNCSRPI
TVTSHDAGCPHCQSHNLRVDGGDSMQIKQIEIE

Sequences:

>Translated_113_residues
MHELSLCQSALDIIERQARDSGAKRVTSVWLDIGALSCIEESALTFCFEIVCRGTLAEGCALRINTVPAQAWCWNCSRPI
TVTSHDAGCPHCQSHNLRVDGGDSMQIKQIEIE
>Mature_113_residues
MHELSLCQSALDIIERQARDSGAKRVTSVWLDIGALSCIEESALTFCFEIVCRGTLAEGCALRINTVPAQAWCWNCSRPI
TVTSHDAGCPHCQSHNLRVDGGDSMQIKQIEIE

Specific function: Probably plays a role in an hydrogenase nickel cofactor insertion step [H]

COG id: COG0375

COG function: function code R; Zn finger protein HypA/HybF (possibly regulating hydrogenase expression)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypA/hybF family [H]

Homologues:

Organism=Escherichia coli, GI1789365, Length=113, Percent_Identity=62.8318584070796, Blast_Score=152, Evalue=4e-39,
Organism=Escherichia coli, GI1789081, Length=114, Percent_Identity=50, Blast_Score=122, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000688
- InterPro:   IPR020538 [H]

Pfam domain/function: PF01155 HypA [H]

EC number: NA

Molecular weight: Translated: 12418; Mature: 12418

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS01249 HYPA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
9.7 %Cys+Met (Translated Protein)
8.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
9.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHELSLCQSALDIIERQARDSGAKRVTSVWLDIGALSCIEESALTFCFEIVCRGTLAEGC
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
ALRINTVPAQAWCWNCSRPITVTSHDAGCPHCQSHNLRVDGGDSMQIKQIEIE
EEEEECCCCCCEEECCCCCEEEECCCCCCCCCCCCCEEECCCCCEEEEEEEEC
>Mature Secondary Structure
MHELSLCQSALDIIERQARDSGAKRVTSVWLDIGALSCIEESALTFCFEIVCRGTLAEGC
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
ALRINTVPAQAWCWNCSRPITVTSHDAGCPHCQSHNLRVDGGDSMQIKQIEIE
EEEEECCCCCCEEECCCCCEEEECCCCCCCCCCCCCEEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]