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The map label for this gene is hypB [H]

Identifier: 238921142

GI number: 238921142

Start: 3162410

End: 3163468

Strand: Direct

Name: hypB [H]

Synonym: NT01EI_3283

Alternate gene names: 238921142

Gene position: 3162410-3163468 (Clockwise)

Preceding gene: 238921141

Following gene: 238921143

Centisome position: 82.95

GC content: 60.15

Gene sequence:

>1059_bases
ATGTGTACCACCTGCGGCTGTGCAGCCGGCGAACTGCATATTGAAGGCGACGAACACCATCACGCCCACCGTCACGACAG
CGACGGTCATGCGCATCACCACGGTCACCACCATCATGGCCATCGCCATGAGAGCCAGGACGCCGCGCGCGTCGTGAATG
TACATCATCACTACCACCATAATGGCGATGTGCACCACCACTATCACTATGTCTTCGCGACGCCTGAACAGGCACAGCAG
CATGCCCACAGTCATGAGCATAACCATGGCCACCATCATGACCATGCGCACGAGCACGGGCATAGCCATGCCGAAGAGAC
GGCGTTCGCGCCCCATCAGCATCACGACGATCTGGACTATGGCCACGGTGAGGCGGGCGCCCACGCGCCGGGCATCAGCC
AGCGCCGCATGGTGCAGATCGAGCAGGACGTACTGAGCAAAAACAACGCGCTGGCCGAGCATAACCGCGAACACTTCACC
CGCCAGCATATTGCTGCGCTGAATCTGGTCTCCAGCCCCGGCTCCGGTAAAACCACCCTGCTGACCGAAACCCTGAAGCG
CATCGCCAGCCGCTTCCCGTGCGCGGTGATCGAAGGCGATCAGCAGACCACCAACGACGCCGAACGCATTCGCGCCACCG
GCGTCAGCGCGATTCAGGTCAACACCGGCAAAGGCTGCCACCTGGATGCTCAGATGGTACATGACGCCATGCACCGCCTG
GCGCTGCCGGACCACAGCCTGCTGTTCATTGAAAACGTCGGCAATCTGGTCTGCCCGGCCAGCTTCGATCTGGGCGAACG
CCATAAAGTCGCGGTGCTGTCTGTCACCGAAGGTGAAGACAAGCCGCTGAAATATCCACATATGTTTGCAGCCTCCAGCC
TGATGATCATCAATAAGATCGATCTGCTACCCTACCTGCAGTTCGACGTCGACGCCTGCATTGCCAACGCGCGACGCATC
AACCCGCAGATCCAGGTGATCCAGCTGTCCGCCACCAGCGGAGAGGGAATGGACGCCTGGCTGGCCTGGCTGGAAACCAC
GCTGGGAGAAAATCAATAA

Upstream 100 bases:

>100_bases
CCGCACTGCCAGAGCCACAATCTGCGCGTCGATGGCGGTGACAGCATGCAAATCAAACAGATTGAGATTGAGTAACCCTA
ACGCTCAGGAGATCCCCTAT

Downstream 100 bases:

>100_bases
TGTGTCTGGGAATTCCGGGAAAAGTGGTCGCCGTCGGCGACGATATCCACCAGCTGGCGCAGGTCGACGTCTGCGGCGTG
AAGCGTGACGTCAACATCGC

Product: hydrogenase nickel incorporation protein HypB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MCTTCGCAAGELHIEGDEHHHAHRHDSDGHAHHHGHHHHGHRHESQDAARVVNVHHHYHHNGDVHHHYHYVFATPEQAQQ
HAHSHEHNHGHHHDHAHEHGHSHAEETAFAPHQHHDDLDYGHGEAGAHAPGISQRRMVQIEQDVLSKNNALAEHNREHFT
RQHIAALNLVSSPGSGKTTLLTETLKRIASRFPCAVIEGDQQTTNDAERIRATGVSAIQVNTGKGCHLDAQMVHDAMHRL
ALPDHSLLFIENVGNLVCPASFDLGERHKVAVLSVTEGEDKPLKYPHMFAASSLMIINKIDLLPYLQFDVDACIANARRI
NPQIQVIQLSATSGEGMDAWLAWLETTLGENQ

Sequences:

>Translated_352_residues
MCTTCGCAAGELHIEGDEHHHAHRHDSDGHAHHHGHHHHGHRHESQDAARVVNVHHHYHHNGDVHHHYHYVFATPEQAQQ
HAHSHEHNHGHHHDHAHEHGHSHAEETAFAPHQHHDDLDYGHGEAGAHAPGISQRRMVQIEQDVLSKNNALAEHNREHFT
RQHIAALNLVSSPGSGKTTLLTETLKRIASRFPCAVIEGDQQTTNDAERIRATGVSAIQVNTGKGCHLDAQMVHDAMHRL
ALPDHSLLFIENVGNLVCPASFDLGERHKVAVLSVTEGEDKPLKYPHMFAASSLMIINKIDLLPYLQFDVDACIANARRI
NPQIQVIQLSATSGEGMDAWLAWLETTLGENQ
>Mature_352_residues
MCTTCGCAAGELHIEGDEHHHAHRHDSDGHAHHHGHHHHGHRHESQDAARVVNVHHHYHHNGDVHHHYHYVFATPEQAQQ
HAHSHEHNHGHHHDHAHEHGHSHAEETAFAPHQHHDDLDYGHGEAGAHAPGISQRRMVQIEQDVLSKNNALAEHNREHFT
RQHIAALNLVSSPGSGKTTLLTETLKRIASRFPCAVIEGDQQTTNDAERIRATGVSAIQVNTGKGCHLDAQMVHDAMHRL
ALPDHSLLFIENVGNLVCPASFDLGERHKVAVLSVTEGEDKPLKYPHMFAASSLMIINKIDLLPYLQFDVDACIANARRI
NPQIQVIQLSATSGEGMDAWLAWLETTLGENQ

Specific function: Required for the maturation of the three [NiFe]hydrogenases. Exhibits a low intrinsic GTPase activity. The GTP hydrolysis catalyzed by HypB is an integral process in the incorporation of nickel into hydrogenases [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=239, Percent_Identity=74.0585774058577, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 39210; Mature: 39210

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTTCGCAAGELHIEGDEHHHAHRHDSDGHAHHHGHHHHGHRHESQDAARVVNVHHHYHH
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
NGDVHHHYHYVFATPEQAQQHAHSHEHNHGHHHDHAHEHGHSHAEETAFAPHQHHDDLDY
CCCEEEEEEEEEECHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHCCCCCCCCCCCCC
GHGEAGAHAPGISQRRMVQIEQDVLSKNNALAEHNREHFTRQHIAALNLVSSPGSGKTTL
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCHH
LTETLKRIASRFPCAVIEGDQQTTNDAERIRATGVSAIQVNTGKGCHLDAQMVHDAMHRL
HHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHH
ALPDHSLLFIENVGNLVCPASFDLGERHKVAVLSVTEGEDKPLKYPHMFAASSLMIINKI
CCCCCCEEEEECCCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHCCEEEEEHH
DLLPYLQFDVDACIANARRINPQIQVIQLSATSGEGMDAWLAWLETTLGENQ
CCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MCTTCGCAAGELHIEGDEHHHAHRHDSDGHAHHHGHHHHGHRHESQDAARVVNVHHHYHH
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
NGDVHHHYHYVFATPEQAQQHAHSHEHNHGHHHDHAHEHGHSHAEETAFAPHQHHDDLDY
CCCEEEEEEEEEECHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHCCCCCCCCCCCCC
GHGEAGAHAPGISQRRMVQIEQDVLSKNNALAEHNREHFTRQHIAALNLVSSPGSGKTTL
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCHH
LTETLKRIASRFPCAVIEGDQQTTNDAERIRATGVSAIQVNTGKGCHLDAQMVHDAMHRL
HHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHH
ALPDHSLLFIENVGNLVCPASFDLGERHKVAVLSVTEGEDKPLKYPHMFAASSLMIINKI
CCCCCCEEEEECCCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHCCEEEEEHH
DLLPYLQFDVDACIANARRINPQIQVIQLSATSGEGMDAWLAWLETTLGENQ
CCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]