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The map label for this gene is dcuB [H]

Identifier: 238921082

GI number: 238921082

Start: 3094845

End: 3096188

Strand: Direct

Name: dcuB [H]

Synonym: NT01EI_3217

Alternate gene names: 238921082

Gene position: 3094845-3096188 (Clockwise)

Preceding gene: 238921080

Following gene: 238921083

Centisome position: 81.18

GC content: 56.55

Gene sequence:

>1344_bases
ATGGTCACACTACAATTTGCCGTTATAATAATCTGTCTATTACTGGGGACACGCTATGGTGGGATGGGGCTGGGGCTTAT
CAGTGGTATTGGTCTCTTCATCCTGACCTTTGTCTTCAATATGGTGCCCGGCAAGCCGCCGGTCGAGGTGATCCTGACCA
TCTTGGCCGTCATCGGTTGCGCCGCCGTGCTACAAACCGCCGGCGGCCTCAATGTAATGATGCAGTTTGCCGAACGCCTG
CTGCGCCGTCATCCGCAACACATCACCATCCTGGCGCCGCTGACCACCTGGATGCTGACATTCCTCTGCGGCACAGGGCA
CGTGGTCTATACCATGTTCCCCATCATTGCCGATATTGCGCTGAAGAAGGGGATCCGCCCAGAGCGTCCGATGGCGGTCG
CCTCCATCGCCTCACAGATGGCCATCACCGCATCGCCAGTTTCCGTCGCCGTGGTCTCACTGGTCTCAATCCTCGGCGCA
GCGCACGGCATTGGCCAGGCCTTCACTATTCTGGATATTCTGGCCGTGTCGGTGCCAGCCTCCCTGTGCGGGGTGCTGGT
CGCCGCGCTTTGGAGCCTGCGCCGCGGTAAAGATCTGGATAAGGATCCGGTATTCCAGGAGAAAATCAAAGATCCGGAGC
AGCGCGCCTACATCTATGGTGATAGCCAGACGCTGCTGAACCAGACGTTCCCCAAACAGGCCTACTGGGCTACCTGGATC
TTTTTTACCGCTATCGCCGTCGTCGTCATCCTGGGCGCCTTCGTCGATCTGCGGCCGGCCTTTGAGGTTAAAGGGGGGAT
GAAACCCCTCTCAATGAACCTGGTCATCCAGATGATGATGCTGATCGCCGGCGCCATCATTCTGATGTCCTGTAAGGTCA
AGCCGGGTGACATCTCCAGCGGTGCCGTCTTTAAAGCCGGCATGGTCGCCATTTTCTCCGTCTTTGGCGTCGCCTGGATG
AGTGATACCTTCTTCCAGGCGCACATGCAGGATCTACGCCTGGTGCTGGAAGATGTCGTGAAGAGCCATCCCTGGACCTA
TGCGCTGGTGCTGTTTTTGGTCTCCAAGCTGGTGAACAGTCAGGCTGCTGCACTGACCGCCATCGCACCGATGGGGCTAT
CGCTGGGCGTAGAACCAAAAATGCTGCTGGCATTCTTTCCGGCGGCCTATGGCTACTTCGTTTTGCCAACCTATCCCAGC
GATCTGGCCTGTATCGGTTTTGACCGCTCAGGTACAACCCGCATCGGGAAATTTATTATTAACCACAGTTTTATCCTGCC
CGGATTTATCGGTGTCATCACCTCGTGTACGGTAGGCTACCTGCTGGTTGCTGCCTTCTATTAA

Upstream 100 bases:

>100_bases
ATTACGCAGCATTACGCTGTATACGATTTTCGCTGTCATGAATACCCGACGCCCTTATACCATGAGGCACGGCGTGGGAA
AATAAACACAAAGGGAACAT

Downstream 100 bases:

>100_bases
GAACGTCCTTACCCGGCCTCCTACGGGAGGTCGATATAAAAAAAGCGTATAACGTTCCCGTCAAGCTTGCAAAAATAAGC
TAATCATTACCATTTTTGGC

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MVTLQFAVIIICLLLGTRYGGMGLGLISGIGLFILTFVFNMVPGKPPVEVILTILAVIGCAAVLQTAGGLNVMMQFAERL
LRRHPQHITILAPLTTWMLTFLCGTGHVVYTMFPIIADIALKKGIRPERPMAVASIASQMAITASPVSVAVVSLVSILGA
AHGIGQAFTILDILAVSVPASLCGVLVAALWSLRRGKDLDKDPVFQEKIKDPEQRAYIYGDSQTLLNQTFPKQAYWATWI
FFTAIAVVVILGAFVDLRPAFEVKGGMKPLSMNLVIQMMMLIAGAIILMSCKVKPGDISSGAVFKAGMVAIFSVFGVAWM
SDTFFQAHMQDLRLVLEDVVKSHPWTYALVLFLVSKLVNSQAAALTAIAPMGLSLGVEPKMLLAFFPAAYGYFVLPTYPS
DLACIGFDRSGTTRIGKFIINHSFILPGFIGVITSCTVGYLLVAAFY

Sequences:

>Translated_447_residues
MVTLQFAVIIICLLLGTRYGGMGLGLISGIGLFILTFVFNMVPGKPPVEVILTILAVIGCAAVLQTAGGLNVMMQFAERL
LRRHPQHITILAPLTTWMLTFLCGTGHVVYTMFPIIADIALKKGIRPERPMAVASIASQMAITASPVSVAVVSLVSILGA
AHGIGQAFTILDILAVSVPASLCGVLVAALWSLRRGKDLDKDPVFQEKIKDPEQRAYIYGDSQTLLNQTFPKQAYWATWI
FFTAIAVVVILGAFVDLRPAFEVKGGMKPLSMNLVIQMMMLIAGAIILMSCKVKPGDISSGAVFKAGMVAIFSVFGVAWM
SDTFFQAHMQDLRLVLEDVVKSHPWTYALVLFLVSKLVNSQAAALTAIAPMGLSLGVEPKMLLAFFPAAYGYFVLPTYPS
DLACIGFDRSGTTRIGKFIINHSFILPGFIGVITSCTVGYLLVAAFY
>Mature_447_residues
MVTLQFAVIIICLLLGTRYGGMGLGLISGIGLFILTFVFNMVPGKPPVEVILTILAVIGCAAVLQTAGGLNVMMQFAERL
LRRHPQHITILAPLTTWMLTFLCGTGHVVYTMFPIIADIALKKGIRPERPMAVASIASQMAITASPVSVAVVSLVSILGA
AHGIGQAFTILDILAVSVPASLCGVLVAALWSLRRGKDLDKDPVFQEKIKDPEQRAYIYGDSQTLLNQTFPKQAYWATWI
FFTAIAVVVILGAFVDLRPAFEVKGGMKPLSMNLVIQMMMLIAGAIILMSCKVKPGDISSGAVFKAGMVAIFSVFGVAWM
SDTFFQAHMQDLRLVLEDVVKSHPWTYALVLFLVSKLVNSQAAALTAIAPMGLSLGVEPKMLLAFFPAAYGYFVLPTYPS
DLACIGFDRSGTTRIGKFIINHSFILPGFIGVITSCTVGYLLVAAFY

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=410, Percent_Identity=57.5609756097561, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1790580, Length=445, Percent_Identity=36.6292134831461, Blast_Score=266, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 48164; Mature: 48164

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTLQFAVIIICLLLGTRYGGMGLGLISGIGLFILTFVFNMVPGKPPVEVILTILAVIGC
CEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AAVLQTAGGLNVMMQFAERLLRRHPQHITILAPLTTWMLTFLCGTGHVVYTMFPIIADIA
HHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LKKGIRPERPMAVASIASQMAITASPVSVAVVSLVSILGAAHGIGQAFTILDILAVSVPA
HHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLCGVLVAALWSLRRGKDLDKDPVFQEKIKDPEQRAYIYGDSQTLLNQTFPKQAYWATWI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHCEEEECCHHHHHHHCCCCHHHHHHHH
FFTAIAVVVILGAFVDLRPAFEVKGGMKPLSMNLVIQMMMLIAGAIILMSCKVKPGDISS
HHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GAVFKAGMVAIFSVFGVAWMSDTFFQAHMQDLRLVLEDVVKSHPWTYALVLFLVSKLVNS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
QAAALTAIAPMGLSLGVEPKMLLAFFPAAYGYFVLPTYPSDLACIGFDRSGTTRIGKFII
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCCHHHHHHHH
NHSFILPGFIGVITSCTVGYLLVAAFY
CCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVTLQFAVIIICLLLGTRYGGMGLGLISGIGLFILTFVFNMVPGKPPVEVILTILAVIGC
CEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AAVLQTAGGLNVMMQFAERLLRRHPQHITILAPLTTWMLTFLCGTGHVVYTMFPIIADIA
HHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LKKGIRPERPMAVASIASQMAITASPVSVAVVSLVSILGAAHGIGQAFTILDILAVSVPA
HHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLCGVLVAALWSLRRGKDLDKDPVFQEKIKDPEQRAYIYGDSQTLLNQTFPKQAYWATWI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHCEEEECCHHHHHHHCCCCHHHHHHHH
FFTAIAVVVILGAFVDLRPAFEVKGGMKPLSMNLVIQMMMLIAGAIILMSCKVKPGDISS
HHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GAVFKAGMVAIFSVFGVAWMSDTFFQAHMQDLRLVLEDVVKSHPWTYALVLFLVSKLVNS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
QAAALTAIAPMGLSLGVEPKMLLAFFPAAYGYFVLPTYPSDLACIGFDRSGTTRIGKFII
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCCHHHHHHHH
NHSFILPGFIGVITSCTVGYLLVAAFY
CCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]