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The map label for this gene is btuF [H]

Identifier: 238920972

GI number: 238920972

Start: 2991768

End: 2992631

Strand: Direct

Name: btuF [H]

Synonym: NT01EI_3102

Alternate gene names: 238920972

Gene position: 2991768-2992631 (Clockwise)

Preceding gene: 238920971

Following gene: 238920973

Centisome position: 78.48

GC content: 68.87

Gene sequence:

>864_bases
ATGGCCCTGCGACGCGCCAAACGCCGCCGCGGATCGGGCGGCATACTGTCGCTGGCTGCGCTGCTGCTGGGCCTCGGTCT
GAGCCTATCCCCGCTGCAGGCGGCGGCGCTGCGCGTCGTCAGCCTGTCGCCGCACACCACCGAACTGGCCTACGCCGCCG
GCCTCGGCGATAGCCTGGTCGCCGCCAGCGCCTATTCCGACTACCCGCCGCAGGCGGCAGGGCTGGAAAAGGTGGCCTCC
TGGAACGGAATCAACCTGGAGCGCATCCTGGCCCTTAAACCCGATCTGATCCTGGCCTGGCACGGCGGCAATCCCCGCCG
CGTCCTCGATCAGCTGAGCACGCTGGGGATCCCCCTCTTCTACTCCGATCCCCAGCGTGTGGATCAGGTGGCTGACGATC
TCGACAGGCTGGCGGCCTATAGCCCTCACCCCGGGCAGGCGCACCGCGCCGCCGCCCAGTTTCGCCAGCGCCTGCAACTG
CTGCGTCAGCGCTACCCCCGGGGCACGCCGCTGCGGGTGATGATGCAGTTTGGCAACCAGCCGCTGTTTTCCACCTCCGC
GAAAACGCTGCAGAGCGATCTGCTGACGCTGTGCGGCGCACAGAATATCTTTGCCGACAGCCCGGTGCCCTGGCCGCAGG
TCAGCCGCGAACAGGTGATGGTGCGCCACCCGCAGGCGATCGTCATCGGCGGCGACGCCGCTCAGGCGGCCATCGTCGCC
GACTATTGGCGCCCCCAGCTCGCGGTGCCGGTGATCGCCGTCAACCAGGACTGGCTCAACCGCAGCGGTCCGCGCATTCT
GCTGGCCGCCGAATCTCTCTGCGCACAACTGCATACGCTGAGCGCCACAGCGCCTCCCCGCTGA

Upstream 100 bases:

>100_bases
GGAGTCCCATATCAGCTTTGATGAGTTCCTCAAGGTCGCCGCCGAGCAGTCTACCCTGATGGTGGAAGCAATGCTGCGCG
CAATGGCCTCCCAGTAATCC

Downstream 100 bases:

>100_bases
GTTCCCGGCGGGTGTGTCCTGCGCCCGCTTTTCTCCCCCCCGCTTGTCAGACAAAACGCCGCTGGATTCGGTGGGATCTT
CCGTTGGCGCGAGCTGACGC

Product: vitamin B12-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MALRRAKRRRGSGGILSLAALLLGLGLSLSPLQAAALRVVSLSPHTTELAYAAGLGDSLVAASAYSDYPPQAAGLEKVAS
WNGINLERILALKPDLILAWHGGNPRRVLDQLSTLGIPLFYSDPQRVDQVADDLDRLAAYSPHPGQAHRAAAQFRQRLQL
LRQRYPRGTPLRVMMQFGNQPLFSTSAKTLQSDLLTLCGAQNIFADSPVPWPQVSREQVMVRHPQAIVIGGDAAQAAIVA
DYWRPQLAVPVIAVNQDWLNRSGPRILLAAESLCAQLHTLSATAPPR

Sequences:

>Translated_287_residues
MALRRAKRRRGSGGILSLAALLLGLGLSLSPLQAAALRVVSLSPHTTELAYAAGLGDSLVAASAYSDYPPQAAGLEKVAS
WNGINLERILALKPDLILAWHGGNPRRVLDQLSTLGIPLFYSDPQRVDQVADDLDRLAAYSPHPGQAHRAAAQFRQRLQL
LRQRYPRGTPLRVMMQFGNQPLFSTSAKTLQSDLLTLCGAQNIFADSPVPWPQVSREQVMVRHPQAIVIGGDAAQAAIVA
DYWRPQLAVPVIAVNQDWLNRSGPRILLAAESLCAQLHTLSATAPPR
>Mature_286_residues
ALRRAKRRRGSGGILSLAALLLGLGLSLSPLQAAALRVVSLSPHTTELAYAAGLGDSLVAASAYSDYPPQAAGLEKVASW
NGINLERILALKPDLILAWHGGNPRRVLDQLSTLGIPLFYSDPQRVDQVADDLDRLAAYSPHPGQAHRAAAQFRQRLQLL
RQRYPRGTPLRVMMQFGNQPLFSTSAKTLQSDLLTLCGAQNIFADSPVPWPQVSREQVMVRHPQAIVIGGDAAQAAIVAD
YWRPQLAVPVIAVNQDWLNRSGPRILLAAESLCAQLHTLSATAPPR

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=246, Percent_Identity=48.780487804878, Blast_Score=250, Evalue=9e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 31058; Mature: 30927

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRRAKRRRGSGGILSLAALLLGLGLSLSPLQAAALRVVSLSPHTTELAYAAGLGDSLV
CCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHEEEEECCCCCHHHHHHHCCCCHHH
AASAYSDYPPQAAGLEKVASWNGINLERILALKPDLILAWHGGNPRRVLDQLSTLGIPLF
HHCCCCCCCCCHHHHHHHHCCCCCCHHHHEEECCCEEEEEECCCHHHHHHHHHHCCCCEE
YSDPQRVDQVADDLDRLAAYSPHPGQAHRAAAQFRQRLQLLRQRYPRGTPLRVMMQFGNQ
ECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
PLFSTSAKTLQSDLLTLCGAQNIFADSPVPWPQVSREQVMVRHPQAIVIGGDAAQAAIVA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHEECCCEEEEECCCHHHHHHH
DYWRPQLAVPVIAVNQDWLNRSGPRILLAAESLCAQLHTLSATAPPR
HHCCCCCCCEEEEECHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ALRRAKRRRGSGGILSLAALLLGLGLSLSPLQAAALRVVSLSPHTTELAYAAGLGDSLV
CCCHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHEEEEECCCCCHHHHHHHCCCCHHH
AASAYSDYPPQAAGLEKVASWNGINLERILALKPDLILAWHGGNPRRVLDQLSTLGIPLF
HHCCCCCCCCCHHHHHHHHCCCCCCHHHHEEECCCEEEEEECCCHHHHHHHHHHCCCCEE
YSDPQRVDQVADDLDRLAAYSPHPGQAHRAAAQFRQRLQLLRQRYPRGTPLRVMMQFGNQ
ECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
PLFSTSAKTLQSDLLTLCGAQNIFADSPVPWPQVSREQVMVRHPQAIVIGGDAAQAAIVA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHEECCCEEEEECCCHHHHHHH
DYWRPQLAVPVIAVNQDWLNRSGPRILLAAESLCAQLHTLSATAPPR
HHCCCCCCCEEEEECHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA