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The map label for this gene is emrB [H]

Identifier: 238920965

GI number: 238920965

Start: 2982504

End: 2984039

Strand: Direct

Name: emrB [H]

Synonym: NT01EI_3095

Alternate gene names: 238920965

Gene position: 2982504-2984039 (Clockwise)

Preceding gene: 238920964

Following gene: 238920966

Centisome position: 78.23

GC content: 59.38

Gene sequence:

>1536_bases
GTGCAACAAAAACCACTTGAGGGAGCCAAACTTGCATGGATGACCATCGCGCTGTCGATGGCCACCTTCATGCAGGTGCT
GGACTCCACGATTGCCAACGTGGCTATCCCGACGATCTCCGGAAACCTGGGCGCCTCCAACTCCCAGGGAACCTGGGTTA
TCACCTCGTTTGGGGTGGCCAACGCCATCTCGATTCCTATCACCGGATGGCTGGCCAAACGCTTTGGCGAGGTACGGCTG
TTTCTGTGGTCAACCGGGCTCTTCGCTCTGACCTCATGGCTGTGCGGAGTTTCCCAAAGCCTGGAGATGCTGATCCTGTT
CCGCGTGCTGCAGGGACTGGTTGCCGGGCCGCTGATCCCGCTCTCCCAGAGCCTGCTGCTCGGTAACTATCCCCCCGCCA
AACGCAATATGGCGCTGGCCCTCTGGTCGATGACGGTGATCGTCGCCCCGATCTGTGGGCCCATACTCGGCGGCTATATC
AGCGATAACTACCACTGGGGCTGGATCTTTTTTATCAACGTGCCTCTGGGGATCGGCGTGGTGCTGGTAAGCATGTCGAC
CCTGAAAGGGCGGGAGACGCGCATAGAGCATAAACCGATCGATACCGTCGGTCTGATCCTGCTGGCGCTGGGGGTCGGCT
GTCTACAGGTGATGCTGGATAAAGGCAAAGAGCTGGACTGGTTTAACTCCTCGGAGATCATCCTGCTGACGGTGGTCGCC
GTGGTGTCACTGATCTTCCTGCTAATCTGGGAGATGACAGACGATCACCCGGTGGTGGATCTCTCGCTGTTTAAAATCCG
CAATTTTACCATCGGCGTCCTGAGCATCAGCCTGGCCTATATGTTCTACTTTGGCGCCATCGTGCTGCTGCCACTGCTGC
TGCAGGAGGTGTACGGCTATACCGCTACCTGGGCCGGGCTGGCCTCGGCACCAGTAGGGCTAATTCCGGTACTGCTCTCC
CCGATCATCGGACGCTTCGGTGCCCGGCTCGATATGCGGCGACTGGTCACCTTCAGCTTCATCATGTATGCCGTCTGTTT
CTACTGGCGTGCCTACAGCTTTGAACCGGGGATGGACTTTGGCGCCTCAGCCTGGCCGCAGTTTGTCCAGGGGTTTGCCG
TAGCCTGCTTCTTTATGCCGCTGACCACCATCAGCCTGTCCGGTCTGCCGCCGGAACGCATTGCAGCAGCCTCCAGTCTG
AGCAACTTCTGCCGTACCCTGGCAGGCTCGATCGGGGTTTCGATCACCACTACGCTGTGGACCCGCCGCGAGGCGCTGCA
CCATACCCACCTAACCGAGTCGATCACCCCTTATAATCCCATCGCCCAGCAGGCCTATGGCGAAATGGAAAAACTGGGGC
TGAACAGCCACCAGGCCTCCGCGCTGATAGCCCAGGACATCACCGCCCAGGGTCTGATCATCTCGGCAAACGAGATCTTT
TGGGCCGCTGCCGGCGTCTTTCTGGTGCTGCTGATATTGATTTGGTTCGCCAAGCCCCCCTTCTCCAGCGCAGGCGACAG
CGGCGGCGCCCACTGA

Upstream 100 bases:

>100_bases
CCTACCAGAGCAATGTACTGACACTGGACCTGGCACCGGTGAATCGCGAGATCGCCGGGATCATCCAGGCCAACGCCGCT
TAATCTGCGCCGAGGTGACT

Downstream 100 bases:

>100_bases
CCCAACGCACCTGCAATCTCTCTCTCAACCGCCCATCCAGGGCGGTTTTTTTAGCATTTCCCCCCGCAGCATCCCCATCC
CTCGGCCGATCATGGTAAAC

Product: multidrug resistance protein Y

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MQQKPLEGAKLAWMTIALSMATFMQVLDSTIANVAIPTISGNLGASNSQGTWVITSFGVANAISIPITGWLAKRFGEVRL
FLWSTGLFALTSWLCGVSQSLEMLILFRVLQGLVAGPLIPLSQSLLLGNYPPAKRNMALALWSMTVIVAPICGPILGGYI
SDNYHWGWIFFINVPLGIGVVLVSMSTLKGRETRIEHKPIDTVGLILLALGVGCLQVMLDKGKELDWFNSSEIILLTVVA
VVSLIFLLIWEMTDDHPVVDLSLFKIRNFTIGVLSISLAYMFYFGAIVLLPLLLQEVYGYTATWAGLASAPVGLIPVLLS
PIIGRFGARLDMRRLVTFSFIMYAVCFYWRAYSFEPGMDFGASAWPQFVQGFAVACFFMPLTTISLSGLPPERIAAASSL
SNFCRTLAGSIGVSITTTLWTRREALHHTHLTESITPYNPIAQQAYGEMEKLGLNSHQASALIAQDITAQGLIISANEIF
WAAAGVFLVLLILIWFAKPPFSSAGDSGGAH

Sequences:

>Translated_511_residues
MQQKPLEGAKLAWMTIALSMATFMQVLDSTIANVAIPTISGNLGASNSQGTWVITSFGVANAISIPITGWLAKRFGEVRL
FLWSTGLFALTSWLCGVSQSLEMLILFRVLQGLVAGPLIPLSQSLLLGNYPPAKRNMALALWSMTVIVAPICGPILGGYI
SDNYHWGWIFFINVPLGIGVVLVSMSTLKGRETRIEHKPIDTVGLILLALGVGCLQVMLDKGKELDWFNSSEIILLTVVA
VVSLIFLLIWEMTDDHPVVDLSLFKIRNFTIGVLSISLAYMFYFGAIVLLPLLLQEVYGYTATWAGLASAPVGLIPVLLS
PIIGRFGARLDMRRLVTFSFIMYAVCFYWRAYSFEPGMDFGASAWPQFVQGFAVACFFMPLTTISLSGLPPERIAAASSL
SNFCRTLAGSIGVSITTTLWTRREALHHTHLTESITPYNPIAQQAYGEMEKLGLNSHQASALIAQDITAQGLIISANEIF
WAAAGVFLVLLILIWFAKPPFSSAGDSGGAH
>Mature_511_residues
MQQKPLEGAKLAWMTIALSMATFMQVLDSTIANVAIPTISGNLGASNSQGTWVITSFGVANAISIPITGWLAKRFGEVRL
FLWSTGLFALTSWLCGVSQSLEMLILFRVLQGLVAGPLIPLSQSLLLGNYPPAKRNMALALWSMTVIVAPICGPILGGYI
SDNYHWGWIFFINVPLGIGVVLVSMSTLKGRETRIEHKPIDTVGLILLALGVGCLQVMLDKGKELDWFNSSEIILLTVVA
VVSLIFLLIWEMTDDHPVVDLSLFKIRNFTIGVLSISLAYMFYFGAIVLLPLLLQEVYGYTATWAGLASAPVGLIPVLLS
PIIGRFGARLDMRRLVTFSFIMYAVCFYWRAYSFEPGMDFGASAWPQFVQGFAVACFFMPLTTISLSGLPPERIAAASSL
SNFCRTLAGSIGVSITTTLWTRREALHHTHLTESITPYNPIAQQAYGEMEKLGLNSHQASALIAQDITAQGLIISANEIF
WAAAGVFLVLLILIWFAKPPFSSAGDSGGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=500, Percent_Identity=80.2, Blast_Score=814, Evalue=0.0,
Organism=Escherichia coli, GI1788710, Length=501, Percent_Identity=63.6726546906188, Blast_Score=636, Evalue=0.0,
Organism=Escherichia coli, GI1790195, Length=438, Percent_Identity=31.2785388127854, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI87081983, Length=422, Percent_Identity=28.436018957346, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=29.2079207920792, Blast_Score=119, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6319770, Length=291, Percent_Identity=26.4604810996564, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=190, Percent_Identity=26.8421052631579, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55580; Mature: 55580

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQKPLEGAKLAWMTIALSMATFMQVLDSTIANVAIPTISGNLGASNSQGTWVITSFGVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEEECCCC
NAISIPITGWLAKRFGEVRLFLWSTGLFALTSWLCGVSQSLEMLILFRVLQGLVAGPLIP
CEEECCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LSQSLLLGNYPPAKRNMALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPLGIGV
CCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCHHHHH
VLVSMSTLKGRETRIEHKPIDTVGLILLALGVGCLQVMLDKGKELDWFNSSEIILLTVVA
HHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VVSLIFLLIWEMTDDHPVVDLSLFKIRNFTIGVLSISLAYMFYFGAIVLLPLLLQEVYGY
HHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TATWAGLASAPVGLIPVLLSPIIGRFGARLDMRRLVTFSFIMYAVCFYWRAYSFEPGMDF
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GASAWPQFVQGFAVACFFMPLTTISLSGLPPERIAAASSLSNFCRTLAGSIGVSITTTLW
CCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHH
TRREALHHTHLTESITPYNPIAQQAYGEMEKLGLNSHQASALIAQDITAQGLIISANEIF
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCHHH
WAAAGVFLVLLILIWFAKPPFSSAGDSGGAH
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQKPLEGAKLAWMTIALSMATFMQVLDSTIANVAIPTISGNLGASNSQGTWVITSFGVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEEECCCC
NAISIPITGWLAKRFGEVRLFLWSTGLFALTSWLCGVSQSLEMLILFRVLQGLVAGPLIP
CEEECCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LSQSLLLGNYPPAKRNMALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPLGIGV
CCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCHHHHH
VLVSMSTLKGRETRIEHKPIDTVGLILLALGVGCLQVMLDKGKELDWFNSSEIILLTVVA
HHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VVSLIFLLIWEMTDDHPVVDLSLFKIRNFTIGVLSISLAYMFYFGAIVLLPLLLQEVYGY
HHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TATWAGLASAPVGLIPVLLSPIIGRFGARLDMRRLVTFSFIMYAVCFYWRAYSFEPGMDF
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GASAWPQFVQGFAVACFFMPLTTISLSGLPPERIAAASSLSNFCRTLAGSIGVSITTTLW
CCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHH
TRREALHHTHLTESITPYNPIAQQAYGEMEKLGLNSHQASALIAQDITAQGLIISANEIF
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCHHH
WAAAGVFLVLLILIWFAKPPFSSAGDSGGAH
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]