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The map label for this gene is dus1 [H]

Identifier: 238917594

GI number: 238917594

Start: 1732835

End: 1733800

Strand: Reverse

Name: dus1 [H]

Synonym: EUBELI_01673

Alternate gene names: 238917594

Gene position: 1733800-1732835 (Counterclockwise)

Preceding gene: 238917595

Following gene: 238917593

Centisome position: 80.86

GC content: 43.69

Gene sequence:

>966_bases
ATGAGAATTGGAAATGTTGAGATAAAGAACAATATTGCATTGGCACCGATGGCAGGAGTAACAGACCTGCCATTTCGACT
TTTATGCAAGGAGCAGGGCTGTGGGCTTATGTGTACAGAGATGGTCAGCGCCAAAGCAGTGCTGTATAACAATAAGAATA
CAGAAGACCTGATGAAGACGGTGCCAGGGGAGCGTCCGCTTGCATTGCAGCTTTTCGGTTCAGACCCTGATATCATGGCA
GATATAGCGAAGAGACTTTCTGAAAGAGAGTTCGATATAATTGATGTCAATATGGGATGTCCTGTTCCAAAGGTAGTCAA
TAATGGCGAAGGTTCGGCACTTATGAAGGACCCTGTGCTTGTCGGAAGAATAGTCGAGAAGATGGCTGATGCACTTGATA
AGCCGCTTACAATCAAGATAAGGGCAGGATTTGATGAGAATAGTATAAATGCGCCTGAGATAGCACATGTTATTGAGGAG
TCTGGCGGGGCTGCCGTTGCAGTCCATGCAAGAACAAGGCAGCAGTATTATTCAGGACATGCAGACTGGGATATAATAAG
ACAGGTCAAAGAAGCAGTATCGATTCCAGTTATTGGTAATGGCGATATTAAAAGCGGCGAAGATGCTGTTAATATGATGA
AGCAGACAGGCTGTGACGGAATAATGATTGGAAGGGCAGCAAGAGGAAATCCATGGATATTCAGACAGGTTTCTGAATAT
ATGAATAATGGTACAATAATTGAACCACCATCCGTTGAAGAAATATGCCAGATGATTAAGAGACATGCCAAAGGACTGTG
TGAGATAAAAGGCGAGTTTACAGGAATCCGCGAGATGAGAAAACATTTTGCATGGTATACAGCAGGAATCAAGCATGCAG
CAGCATTAAGAAATGAAGCTAACCAGGTTACTAATCTGGAGCAGTTTGATGGGCTTGTGGACATGATACTGGAAGGGCTG
CATTGA

Upstream 100 bases:

>100_bases
CATGCGGTATGCTCGTCAAGAAGTATCAGTATGAGAAGAGAGAGCTTCTTGAATCACTTGAGATGTTCTTCAAGGTATTC
TTCTTAGGCATGGAGTAATT

Downstream 100 bases:

>100_bases
CAGACAGGAACTGATAGTTTATAATATTGTGACGCTTGTGGGGTTTTATTCTCTAGTAGAGCAGAAGCATATTAGTGTAG
CATATATATAGAAAGGTGAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRIGNVEIKNNIALAPMAGVTDLPFRLLCKEQGCGLMCTEMVSAKAVLYNNKNTEDLMKTVPGERPLALQLFGSDPDIMA
DIAKRLSEREFDIIDVNMGCPVPKVVNNGEGSALMKDPVLVGRIVEKMADALDKPLTIKIRAGFDENSINAPEIAHVIEE
SGGAAVAVHARTRQQYYSGHADWDIIRQVKEAVSIPVIGNGDIKSGEDAVNMMKQTGCDGIMIGRAARGNPWIFRQVSEY
MNNGTIIEPPSVEEICQMIKRHAKGLCEIKGEFTGIREMRKHFAWYTAGIKHAAALRNEANQVTNLEQFDGLVDMILEGL
H

Sequences:

>Translated_321_residues
MRIGNVEIKNNIALAPMAGVTDLPFRLLCKEQGCGLMCTEMVSAKAVLYNNKNTEDLMKTVPGERPLALQLFGSDPDIMA
DIAKRLSEREFDIIDVNMGCPVPKVVNNGEGSALMKDPVLVGRIVEKMADALDKPLTIKIRAGFDENSINAPEIAHVIEE
SGGAAVAVHARTRQQYYSGHADWDIIRQVKEAVSIPVIGNGDIKSGEDAVNMMKQTGCDGIMIGRAARGNPWIFRQVSEY
MNNGTIIEPPSVEEICQMIKRHAKGLCEIKGEFTGIREMRKHFAWYTAGIKHAAALRNEANQVTNLEQFDGLVDMILEGL
H
>Mature_321_residues
MRIGNVEIKNNIALAPMAGVTDLPFRLLCKEQGCGLMCTEMVSAKAVLYNNKNTEDLMKTVPGERPLALQLFGSDPDIMA
DIAKRLSEREFDIIDVNMGCPVPKVVNNGEGSALMKDPVLVGRIVEKMADALDKPLTIKIRAGFDENSINAPEIAHVIEE
SGGAAVAVHARTRQQYYSGHADWDIIRQVKEAVSIPVIGNGDIKSGEDAVNMMKQTGCDGIMIGRAARGNPWIFRQVSEY
MNNGTIIEPPSVEEICQMIKRHAKGLCEIKGEFTGIREMRKHFAWYTAGIKHAAALRNEANQVTNLEQFDGLVDMILEGL
H

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=240, Percent_Identity=38.75, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI239788462, Length=237, Percent_Identity=38.8185654008439, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI31742496, Length=221, Percent_Identity=30.7692307692308, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI40807366, Length=226, Percent_Identity=32.7433628318584, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI8923374, Length=246, Percent_Identity=30.8943089430894, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1789660, Length=288, Percent_Identity=42.0138888888889, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1788462, Length=313, Percent_Identity=30.0319488817891, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI145693211, Length=315, Percent_Identity=29.5238095238095, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17543114, Length=233, Percent_Identity=36.0515021459227, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17507177, Length=237, Percent_Identity=30.8016877637131, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI25144369, Length=222, Percent_Identity=30.1801801801802, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=245, Percent_Identity=29.7959183673469, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=279, Percent_Identity=30.1075268817204, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323433, Length=305, Percent_Identity=27.2131147540984, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6323437, Length=224, Percent_Identity=28.125, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24585320, Length=239, Percent_Identity=35.5648535564854, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24580595, Length=228, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19920448, Length=228, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI45549423, Length=251, Percent_Identity=31.4741035856574, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19921524, Length=224, Percent_Identity=30.3571428571429, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35288; Mature: 35288

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGNVEIKNNIALAPMAGVTDLPFRLLCKEQGCGLMCTEMVSAKAVLYNNKNTEDLMKT
CCCCCEEECCCEEECCCCCCCCCCHHHHHHCCCCCEEHHHHHHHHEEEECCCCHHHHHHH
VPGERPLALQLFGSDPDIMADIAKRLSEREFDIIDVNMGCPVPKVVNNGEGSALMKDPVL
CCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCCCEEECCHHH
VGRIVEKMADALDKPLTIKIRAGFDENSINAPEIAHVIEESGGAAVAVHARTRQQYYSGH
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHCCC
ADWDIIRQVKEAVSIPVIGNGDIKSGEDAVNMMKQTGCDGIMIGRAARGNPWIFRQVSEY
CCHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHH
MNNGTIIEPPSVEEICQMIKRHAKGLCEIKGEFTGIREMRKHFAWYTAGIKHAAALRNEA
HCCCCEECCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQVTNLEQFDGLVDMILEGLH
HHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIGNVEIKNNIALAPMAGVTDLPFRLLCKEQGCGLMCTEMVSAKAVLYNNKNTEDLMKT
CCCCCEEECCCEEECCCCCCCCCCHHHHHHCCCCCEEHHHHHHHHEEEECCCCHHHHHHH
VPGERPLALQLFGSDPDIMADIAKRLSEREFDIIDVNMGCPVPKVVNNGEGSALMKDPVL
CCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCCCEEECCHHH
VGRIVEKMADALDKPLTIKIRAGFDENSINAPEIAHVIEESGGAAVAVHARTRQQYYSGH
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHCCC
ADWDIIRQVKEAVSIPVIGNGDIKSGEDAVNMMKQTGCDGIMIGRAARGNPWIFRQVSEY
CCHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHH
MNNGTIIEPPSVEEICQMIKRHAKGLCEIKGEFTGIREMRKHFAWYTAGIKHAAALRNEA
HCCCCEECCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQVTNLEQFDGLVDMILEGLH
HHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]