Click here to switch to the map view.

The map label for this gene is greA [H]

Identifier: 238917593

GI number: 238917593

Start: 1732252

End: 1732728

Strand: Reverse

Name: greA [H]

Synonym: EUBELI_01672

Alternate gene names: 238917593

Gene position: 1732728-1732252 (Counterclockwise)

Preceding gene: 238917594

Following gene: 238917592

Centisome position: 80.81

GC content: 40.67

Gene sequence:

>477_bases
ATGGCAGAGAAACAGTTTCTTACTAAAAAGGGGTATGACGAAAGAGTAGCAAGACTTGAGGAGCTTAAGGTTGTCAGAAG
AAAGGAAGTTGCCCAGAAGTTAAAGGAAGCAAGAGAACAGGGCGATTTATCAGAGAACGCTGAGTACGATGCAGCTAAGG
ATGAGCAGAGAGATATCGAAACAGAAATCGCAGAGCTTGAAGCAATTCTTGCTAACTGTGAGGTTATTCAGGATAACGAT
AAGAATAAAGATGTAGTTAAGATGGAGAGCGTTGTTGTACTTCACGATGTAGAAATCGATGAGGATATTGAGTACACAAT
CGTTGGTTCATCAGAGGCAGACAGCCTTAATAACAAGATTTCTAATGAGTCACCACTTGGTGCAGCTCTTATTGGAAAGA
AGAAGGGTGACACTGTTAAGGTAGAAGCACCTGTTGGAGAGATTGAATATACAGTTGTATCTGTAAAGAACGACTAA

Upstream 100 bases:

>100_bases
GGAACTGATAGTTTATAATATTGTGACGCTTGTGGGGTTTTATTCTCTAGTAGAGCAGAAGCATATTAGTGTAGCATATA
TATAGAAAGGTGATAAGAAC

Downstream 100 bases:

>100_bases
TTTTCAGTAACATATAGAAAAGGAGATACTCATTCATGGGAGATAATAAATCTGCACAGCAGCCTGACGTTAACGAGCAG
ATTAAGGTTCGTATGGAAAA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MAEKQFLTKKGYDERVARLEELKVVRRKEVAQKLKEAREQGDLSENAEYDAAKDEQRDIETEIAELEAILANCEVIQDND
KNKDVVKMESVVVLHDVEIDEDIEYTIVGSSEADSLNNKISNESPLGAALIGKKKGDTVKVEAPVGEIEYTVVSVKND

Sequences:

>Translated_158_residues
MAEKQFLTKKGYDERVARLEELKVVRRKEVAQKLKEAREQGDLSENAEYDAAKDEQRDIETEIAELEAILANCEVIQDND
KNKDVVKMESVVVLHDVEIDEDIEYTIVGSSEADSLNNKISNESPLGAALIGKKKGDTVKVEAPVGEIEYTVVSVKND
>Mature_157_residues
AEKQFLTKKGYDERVARLEELKVVRRKEVAQKLKEAREQGDLSENAEYDAAKDEQRDIETEIAELEAILANCEVIQDNDK
NKDVVKMESVVVLHDVEIDEDIEYTIVGSSEADSLNNKISNESPLGAALIGKKKGDTVKVEAPVGEIEYTVVSVKND

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=151, Percent_Identity=41.7218543046358, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI87082261, Length=152, Percent_Identity=29.6052631578947, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17652; Mature: 17521

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKQFLTKKGYDERVARLEELKVVRRKEVAQKLKEAREQGDLSENAEYDAAKDEQRDIE
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
TEIAELEAILANCEVIQDNDKNKDVVKMESVVVLHDVEIDEDIEYTIVGSSEADSLNNKI
HHHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEEEECCCCCCCEEEEECCCCCHHHHHCC
SNESPLGAALIGKKKGDTVKVEAPVGEIEYTVVSVKND
CCCCCCCEEEECCCCCCEEEEECCCCCEEEEEEEEECC
>Mature Secondary Structure 
AEKQFLTKKGYDERVARLEELKVVRRKEVAQKLKEAREQGDLSENAEYDAAKDEQRDIE
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
TEIAELEAILANCEVIQDNDKNKDVVKMESVVVLHDVEIDEDIEYTIVGSSEADSLNNKI
HHHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEEEECCCCCCCEEEEECCCCCHHHHHCC
SNESPLGAALIGKKKGDTVKVEAPVGEIEYTVVSVKND
CCCCCCCEEEECCCCCCEEEEECCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA