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The map label for this gene is pfkB [H]

Identifier: 238895239

GI number: 238895239

Start: 3120236

End: 3121168

Strand: Direct

Name: pfkB [H]

Synonym: KP1_3290

Alternate gene names: 238895239

Gene position: 3120236-3121168 (Clockwise)

Preceding gene: 238895228

Following gene: 238895240

Centisome position: 59.45

GC content: 62.81

Gene sequence:

>933_bases
ATGACCAAAATTTACACGCTCACGCTGGCCCCCTCGCTGGATAGCGCCACGCAAACCCCACAGATCTACCCGGAAGGCAA
ACTTCGCTGCAGCGCGCCGGTATTTGAACCTGGCGGCGGCGGGATCAACGTGGCCCGCGCCGTGACCTTTCTCGGCGGCA
AAGCCACCGCCATTTTCCCGGTCGGTGGCGCTACCGGCGAGCATCTTGCCGCCCTGCTGGCCGATGAGCAGGTGCCGGTC
GAGACCGTCGAAACCCGCGACTGGACGCGGCAAAACCTGCACGTCCACGTGGCCGCCAGCGGCGAACAGTATCGCTTCGT
TATGCCCGGCGCGGCACTGACGGACGATGAATTCCGCCGTCTGGAAGAGAAGGTTCTGACTATCGAACCCGGCTCTCTGC
TGGTGGTCAGCGGCAGCCTGCCGCCAGGTATTAGCGTCGATAATCTGATGCAACTGGTGAAAAATGCGCAACAGCAGGGA
CTGCGCTGCATCATTGACAGTTCCGGCGATGCGCTGGCCGCCGCGCTGGACGTCGGCAATATTGAGCTGGTGAAACCGAA
CCAGAAAGAGCTTAGCGCCCTCGTGCAGCGCGATCTCAGCCAACCCGATGACGTGCGCCTTGCCGCTCAGTCGTTGATCC
AGTCCGGTAAAGTGCGCCGGGTGGTGGTCTCCCTGGGTCCGCAGGGGGCGTTAGGCGTGGACGCCAGCGGCAGCGTTCAG
GTAGTGCCGCCACCGATGAAAAGCCAGAGCACCGTCGGCGCTGGCGACAGCATGGTGGGCGCCATGACCTTGCGTCTGGC
GGAAAACGCCAGCCTTGAAGACATGGTACGCTTTGGCGTCGCCGCCGGTAGCGCCGCGACCATCAACCAGGGCACCCGCC
TCTGCTCGCGGACCAATACGCAAAAAATCTATGATTATCTCTGCGGGCGTTGA

Upstream 100 bases:

>100_bases
GAGGCATCTTTGCAAAACTGTCAGGAACTGGACTTTCCCTCCCCGAGTCATCGTGCCATGCTTAAATTAACGACAGTCGC
CGTATTAAGGAGGAGAAGGA

Downstream 100 bases:

>100_bases
TCTTCCCGGCCCCCTCCGCGCCGGCGGAGGGGAATCACAATATAAGCAGGATGGTCTATGCTGGAAAGGCCATGCATAAC
AACGGGGTGTAGAGATGAGC

Product: 6-phosphofructokinase 2

Products: NA

Alternate protein names: Phosphofructokinase-2 [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTKIYTLTLAPSLDSATQTPQIYPEGKLRCSAPVFEPGGGGINVARAVTFLGGKATAIFPVGGATGEHLAALLADEQVPV
ETVETRDWTRQNLHVHVAASGEQYRFVMPGAALTDDEFRRLEEKVLTIEPGSLLVVSGSLPPGISVDNLMQLVKNAQQQG
LRCIIDSSGDALAAALDVGNIELVKPNQKELSALVQRDLSQPDDVRLAAQSLIQSGKVRRVVVSLGPQGALGVDASGSVQ
VVPPPMKSQSTVGAGDSMVGAMTLRLAENASLEDMVRFGVAAGSAATINQGTRLCSRTNTQKIYDYLCGR

Sequences:

>Translated_310_residues
MTKIYTLTLAPSLDSATQTPQIYPEGKLRCSAPVFEPGGGGINVARAVTFLGGKATAIFPVGGATGEHLAALLADEQVPV
ETVETRDWTRQNLHVHVAASGEQYRFVMPGAALTDDEFRRLEEKVLTIEPGSLLVVSGSLPPGISVDNLMQLVKNAQQQG
LRCIIDSSGDALAAALDVGNIELVKPNQKELSALVQRDLSQPDDVRLAAQSLIQSGKVRRVVVSLGPQGALGVDASGSVQ
VVPPPMKSQSTVGAGDSMVGAMTLRLAENASLEDMVRFGVAAGSAATINQGTRLCSRTNTQKIYDYLCGR
>Mature_309_residues
TKIYTLTLAPSLDSATQTPQIYPEGKLRCSAPVFEPGGGGINVARAVTFLGGKATAIFPVGGATGEHLAALLADEQVPVE
TVETRDWTRQNLHVHVAASGEQYRFVMPGAALTDDEFRRLEEKVLTIEPGSLLVVSGSLPPGISVDNLMQLVKNAQQQGL
RCIIDSSGDALAAALDVGNIELVKPNQKELSALVQRDLSQPDDVRLAAQSLIQSGKVRRVVVSLGPQGALGVDASGSVQV
VPPPMKSQSTVGAGDSMVGAMTLRLAENASLEDMVRFGVAAGSAATINQGTRLCSRTNTQKIYDYLCGR

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=307, Percent_Identity=79.1530944625407, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1788493, Length=278, Percent_Identity=32.7338129496403, Blast_Score=104, Evalue=8e-24,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 32620; Mature: 32488

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIYTLTLAPSLDSATQTPQIYPEGKLRCSAPVFEPGGGGINVARAVTFLGGKATAIFP
CCEEEEEEECCCCCCCCCCCEECCCCCEEECCCCCCCCCCCEEHHHHHHHCCCCEEEEEE
VGGATGEHLAALLADEQVPVETVETRDWTRQNLHVHVAASGEQYRFVMPGAALTDDEFRR
CCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHHHH
LEEKVLTIEPGSLLVVSGSLPPGISVDNLMQLVKNAQQQGLRCIIDSSGDALAAALDVGN
HHHHEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCC
IELVKPNQKELSALVQRDLSQPDDVRLAAQSLIQSGKVRRVVVSLGPQGALGVDASGSVQ
EEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCEE
VVPPPMKSQSTVGAGDSMVGAMTLRLAENASLEDMVRFGVAAGSAATINQGTRLCSRTNT
EECCCCCCCCCCCCCCCHHHHEEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHCCCH
QKIYDYLCGR
HHHHHHHCCC
>Mature Secondary Structure 
TKIYTLTLAPSLDSATQTPQIYPEGKLRCSAPVFEPGGGGINVARAVTFLGGKATAIFP
CEEEEEEECCCCCCCCCCCEECCCCCEEECCCCCCCCCCCEEHHHHHHHCCCCEEEEEE
VGGATGEHLAALLADEQVPVETVETRDWTRQNLHVHVAASGEQYRFVMPGAALTDDEFRR
CCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHHHH
LEEKVLTIEPGSLLVVSGSLPPGISVDNLMQLVKNAQQQGLRCIIDSSGDALAAALDVGN
HHHHEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCC
IELVKPNQKELSALVQRDLSQPDDVRLAAQSLIQSGKVRRVVVSLGPQGALGVDASGSVQ
EEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCEE
VVPPPMKSQSTVGAGDSMVGAMTLRLAENASLEDMVRFGVAAGSAATINQGTRLCSRTNT
EECCCCCCCCCCCCCCCHHHHEEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHCCCH
QKIYDYLCGR
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6235149; 9097039; 9278503; 6310120 [H]