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The map label for this gene is htrB [H]

Identifier: 238893508

GI number: 238893508

Start: 1351389

End: 1352309

Strand: Direct

Name: htrB [H]

Synonym: KP1_1406

Alternate gene names: 238893508

Gene position: 1351389-1352309 (Clockwise)

Preceding gene: 238893506

Following gene: 238893509

Centisome position: 25.75

GC content: 59.07

Gene sequence:

>921_bases
ATGTCAAATTTACCCAAATTTTCCCGCGCGCTGTTGCACCCGCGTTACTGGCTATTGTGGCTGGGAATTGGCTTTTTGTG
GCTGCTGGTGCAATTACCGTATCCGGTGATCTACCGTCTGGGAACGGCGCTTGGGCGGCTGGCAATGCGGGTGATGAAAA
GCCGCGCGCGGATTGCCAGACGTAATCTCGAACTCTGCTTTCCCGAGATGAGCGCCGCCGACCGCGAGGCGCTGCTGGTG
AAAAACTTTGAATCGCTGGGCATGGGGCTGATGGAGACCGGCATGGCCTGGTTCTGGCCGACGCACCGGGTAGCGCGCTG
GACCGAGACCAGCGGCGTTAACGAGGTGGTGGAGCTTCTGGAAGAAAAGCAGGGCATTTTACTGATCGGTATCCATTTTC
TGACCCTCGAAATGGGTGCGCGAATGTACGGCATGTTTACCCCAGGCATTGGCGTCTACCGGCCCAACGATAACCCGCTG
ATTGACTGGCTGCAAACCTGGGGACGCTTGCGCTCTAACAAGACCATGTTGGATCGAAAAAATCTGAAAGGGATGGTCAG
GGCGCTAAAAGAGGGGGAAATTCTCTGGTACGCGCCGGATCATGACTACGGTCCGGCCAGCAGCGTCTTCGCCCCGCTGT
TTGCCGTCGAACAGGCGGCGACCACCACCGGTACCTGGATGCTGGCGAAAATGTCCGGGGCGACGATTGTGCCGTTTGTT
CCGCGGCGCAAGCCCAACGGCATGGGGTATGAATTGATCTCCCTGACGCCGGAGCGCACGCCGCCGCTGGCGAGCGCCGA
GGTTACGGCGGCCTGGATGAATCAGATTATCGAACAGTGCATCCTGATGGCGCCCGAGCAATACATGTGGCTGCACCGGC
GCTTTAAGACCCGCCCGGAAGGAGTACCGCCGCGTTACTGA

Upstream 100 bases:

>100_bases
GAGTATTACCGCACGAATTGGAAAAATACTACGTGCAGGATCCGCTACTTTATTTCGCGGTTTTTGCTCAGCTATTATTG
AGTGAATTAACAGGATTACT

Downstream 100 bases:

>100_bases
ACGCCTCGGCCAAAAAATAAGCGCATCGGTAGCACGCTCAGTAAATCGCCGTCTATACTGAAAGGGCCTGACTGAAGGCT
AATTCCAAGGAGATTGCAGG

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSNLPKFSRALLHPRYWLLWLGIGFLWLLVQLPYPVIYRLGTALGRLAMRVMKSRARIARRNLELCFPEMSAADREALLV
KNFESLGMGLMETGMAWFWPTHRVARWTETSGVNEVVELLEEKQGILLIGIHFLTLEMGARMYGMFTPGIGVYRPNDNPL
IDWLQTWGRLRSNKTMLDRKNLKGMVRALKEGEILWYAPDHDYGPASSVFAPLFAVEQAATTTGTWMLAKMSGATIVPFV
PRRKPNGMGYELISLTPERTPPLASAEVTAAWMNQIIEQCILMAPEQYMWLHRRFKTRPEGVPPRY

Sequences:

>Translated_306_residues
MSNLPKFSRALLHPRYWLLWLGIGFLWLLVQLPYPVIYRLGTALGRLAMRVMKSRARIARRNLELCFPEMSAADREALLV
KNFESLGMGLMETGMAWFWPTHRVARWTETSGVNEVVELLEEKQGILLIGIHFLTLEMGARMYGMFTPGIGVYRPNDNPL
IDWLQTWGRLRSNKTMLDRKNLKGMVRALKEGEILWYAPDHDYGPASSVFAPLFAVEQAATTTGTWMLAKMSGATIVPFV
PRRKPNGMGYELISLTPERTPPLASAEVTAAWMNQIIEQCILMAPEQYMWLHRRFKTRPEGVPPRY
>Mature_305_residues
SNLPKFSRALLHPRYWLLWLGIGFLWLLVQLPYPVIYRLGTALGRLAMRVMKSRARIARRNLELCFPEMSAADREALLVK
NFESLGMGLMETGMAWFWPTHRVARWTETSGVNEVVELLEEKQGILLIGIHFLTLEMGARMYGMFTPGIGVYRPNDNPLI
DWLQTWGRLRSNKTMLDRKNLKGMVRALKEGEILWYAPDHDYGPASSVFAPLFAVEQAATTTGTWMLAKMSGATIVPFVP
RRKPNGMGYELISLTPERTPPLASAEVTAAWMNQIIEQCILMAPEQYMWLHRRFKTRPEGVPPRY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=306, Percent_Identity=76.4705882352941, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI87082094, Length=301, Percent_Identity=51.8272425249169, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1788161, Length=312, Percent_Identity=27.5641025641026, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35057; Mature: 34926

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLPKFSRALLHPRYWLLWLGIGFLWLLVQLPYPVIYRLGTALGRLAMRVMKSRARIAR
CCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLELCFPEMSAADREALLVKNFESLGMGLMETGMAWFWPTHRVARWTETSGVNEVVELL
HCHHHCCCCCCCCCHHHHHHHCHHHHCCHHHHHCCHHCCCCHHHHHHHHCCCHHHHHHHH
EEKQGILLIGIHFLTLEMGARMYGMFTPGIGVYRPNDNPLIDWLQTWGRLRSNKTMLDRK
HHCCCEEEEHHHHHHHHHCCHHEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHH
NLKGMVRALKEGEILWYAPDHDYGPASSVFAPLFAVEQAATTTGTWMLAKMSGATIVPFV
HHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCC
PRRKPNGMGYELISLTPERTPPLASAEVTAAWMNQIIEQCILMAPEQYMWLHRRFKTRPE
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GVPPRY
CCCCCC
>Mature Secondary Structure 
SNLPKFSRALLHPRYWLLWLGIGFLWLLVQLPYPVIYRLGTALGRLAMRVMKSRARIAR
CCCCHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLELCFPEMSAADREALLVKNFESLGMGLMETGMAWFWPTHRVARWTETSGVNEVVELL
HCHHHCCCCCCCCCHHHHHHHCHHHHCCHHHHHCCHHCCCCHHHHHHHHCCCHHHHHHHH
EEKQGILLIGIHFLTLEMGARMYGMFTPGIGVYRPNDNPLIDWLQTWGRLRSNKTMLDRK
HHCCCEEEEHHHHHHHHHCCHHEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHH
NLKGMVRALKEGEILWYAPDHDYGPASSVFAPLFAVEQAATTTGTWMLAKMSGATIVPFV
HHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCC
PRRKPNGMGYELISLTPERTPPLASAEVTAAWMNQIIEQCILMAPEQYMWLHRRFKTRPE
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GVPPRY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]