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The map label for this gene is gcl [H]

Identifier: 238893468

GI number: 238893468

Start: 1312757

End: 1314538

Strand: Direct

Name: gcl [H]

Synonym: KP1_1361

Alternate gene names: 238893468

Gene position: 1312757-1314538 (Clockwise)

Preceding gene: 238893467

Following gene: 238893469

Centisome position: 25.01

GC content: 56.17

Gene sequence:

>1782_bases
ATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTTCTGGAAAAAGAAGGGGTTAACACGGCATTTGGCGTGCCTGG
CGCGGCTATTAACCCGTTTTATTCTGCGATGCGTAAGCACGGCGGGATCCGCCATATTCTTGCCCGCCATGTTGAAGGCG
CGTCTCATATGGCTGAAGGCTATTCCCGCGCGACTGCCGGTAATATTGGTGTCTGTTTGGGGACCTCCGGTCCGGCGGGT
ACGGATATGATCACCGCCCTGTACTCTGCCTCCGCGGACTCGATTCCGATTCTGTGCATCACCGGCCAGGCGCCGCGCGC
GCGCCTGCATAAAGAGGATTTTCAGGCGGTGGATATTGAATCTATTGCTAAACCAGTTACCAAGATGGCGGTGACTGTCC
GAGAGGCGGCGCTAGTTCCGGGCGTGCTGCAGCAGGCTTTCCATCTGATGCGCTCCGGTAGACCGGGACCGGTGCTCATC
GATTTGCCTTATGATGTCCAGGTGGCAGAAATCGAATTCGACCCTGAGCTTTACTCGCCGCTGCCGGCTTATAAACCGAT
CGCCACGCGTCCGCAAATTGAAAAGGCACTGGCGCTGTTAGTCCAGGCTGAACGTCCGGTGATTGTTGCCGGTGGCGGGG
TGATTAACGCCGATGCCGCCGCGCTGCTGCAACAATTTGCGGAAATCACCCATGTCCCGGTGATCCCGACCCTGATGGGC
TGGGGCTGTATACCGGACGACCACGAACTGATGGCCGGAATGGTCGGCTTACAAACCGCGCATCGCTACGGCAACGCCAC
GTTGTTGGCCTCAGATCTGGTGTTCGGGATTGGCAACCGCTTTGCGAATCGTCATACCGGCTCGGTTGAGAAGTACACCG
CAGGCCGCAAAATTATTCATATCGACATTGAACCGACGCAGATTGGCCGTGTACTGTGCCCGGACTTAGGCATTGTCTCC
GATGCCAAAGCGGCGTTAATTCAGCTGGTAGACATTGCGCAGCAAATGCAAAAAGAGGGTCGCCTGCCGCGTCGCGAGAG
CTGGGTTAGCGAGTGTCAGGCGCGCAAGCGTACGCTGCTGCGTAAAACCCACTTCGATAACGTGCCGGTTAAACCGCAGC
GCGTCTATGAAGAGATGAATAAAGCGTTTGGCCGGGACGTATGCTATGTCACCACCATTGGCCTGTCGCAAATCGCCGCC
GCGCAGATGCTGCATGTGTTCAAAGACCGCCACTGGATCAACTGCGGTCAGGCGGGGCCGCTGGGCTGGACGCTTCCCGC
TGCGTTGGGCGTTTGCGCTGCCGATCCTGAGCGCAACGTGGTCGCGATTTCCGGCGACTTCGACTTCCAGTTTCTGATTG
AAGAGCTGGCGGTAGGCGCCCAGTTCAATATTCCCTATATCCATGTCCTGGTGAACAACGCCTATCTCGGCTTGATCCGC
CAGTCGCAACGCGCCTTTGACATGGACTACTGCGTTCAGCTGGCGTTTGAAAATATTAACTCCAGCGAAGTAAACGGCTA
CGGCGTCGATCATGTGAAGGTCGCGGAGGGACTCGGTTGTAAAGCAATTCGCGTCTTCAAGCCTGAGGATATTGCCCCCG
CGTTTGAGCAGGCCAAAGCATGGATGAAAGCCTATCGCGTGCCGGTCGTTGTGGAAGTGATCCTCGAGCGCGTAACCAAT
ATCTCCATGGGCAGCGAACTGGATAATGTCATGGAGTTCGAAGACGTTGCCGATAGCGCCATTGACGCACCAACCGAAAC
TTGTTTCATGAAATATGAATAA

Upstream 100 bases:

>100_bases
CCAGGCCGACTGATCGCCGATCCAACCCGCTCACTTGAGCGGGACTTTTTTGATTATGTTTATATTTGGAAAAATTTTCC
AATAAAGTGAGGTTGTAAAA

Downstream 100 bases:

>100_bases
ATGAGGTGACATTATGTTGCGCTTTTCTGCCAACCTTTCCATGTTATTTCAGGAATATGATTTTCTGGATCGCTTTGAGA
AAGCGGCGGCCTGTGGTTTT

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MAKMRAVDAAMYVLEKEGVNTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYSRATAGNIGVCLGTSGPAG
TDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIESIAKPVTKMAVTVREAALVPGVLQQAFHLMRSGRPGPVLI
DLPYDVQVAEIEFDPELYSPLPAYKPIATRPQIEKALALLVQAERPVIVAGGGVINADAAALLQQFAEITHVPVIPTLMG
WGCIPDDHELMAGMVGLQTAHRYGNATLLASDLVFGIGNRFANRHTGSVEKYTAGRKIIHIDIEPTQIGRVLCPDLGIVS
DAKAALIQLVDIAQQMQKEGRLPRRESWVSECQARKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAA
AQMLHVFKDRHWINCGQAGPLGWTLPAALGVCAADPERNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKAWMKAYRVPVVVEVILERVTN
ISMGSELDNVMEFEDVADSAIDAPTETCFMKYE

Sequences:

>Translated_593_residues
MAKMRAVDAAMYVLEKEGVNTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYSRATAGNIGVCLGTSGPAG
TDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIESIAKPVTKMAVTVREAALVPGVLQQAFHLMRSGRPGPVLI
DLPYDVQVAEIEFDPELYSPLPAYKPIATRPQIEKALALLVQAERPVIVAGGGVINADAAALLQQFAEITHVPVIPTLMG
WGCIPDDHELMAGMVGLQTAHRYGNATLLASDLVFGIGNRFANRHTGSVEKYTAGRKIIHIDIEPTQIGRVLCPDLGIVS
DAKAALIQLVDIAQQMQKEGRLPRRESWVSECQARKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAA
AQMLHVFKDRHWINCGQAGPLGWTLPAALGVCAADPERNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKAWMKAYRVPVVVEVILERVTN
ISMGSELDNVMEFEDVADSAIDAPTETCFMKYE
>Mature_592_residues
AKMRAVDAAMYVLEKEGVNTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYSRATAGNIGVCLGTSGPAGT
DMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIESIAKPVTKMAVTVREAALVPGVLQQAFHLMRSGRPGPVLID
LPYDVQVAEIEFDPELYSPLPAYKPIATRPQIEKALALLVQAERPVIVAGGGVINADAAALLQQFAEITHVPVIPTLMGW
GCIPDDHELMAGMVGLQTAHRYGNATLLASDLVFGIGNRFANRHTGSVEKYTAGRKIIHIDIEPTQIGRVLCPDLGIVSD
AKAALIQLVDIAQQMQKEGRLPRRESWVSECQARKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAA
QMLHVFKDRHWINCGQAGPLGWTLPAALGVCAADPERNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIRQ
SQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKAWMKAYRVPVVVEVILERVTNI
SMGSELDNVMEFEDVADSAIDAPTETCFMKYE

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=479, Percent_Identity=25.4697286012526, Blast_Score=120, Evalue=5e-27,
Organism=Homo sapiens, GI21361361, Length=596, Percent_Identity=23.3221476510067, Blast_Score=105, Evalue=2e-22,
Organism=Escherichia coli, GI1786717, Length=593, Percent_Identity=92.4114671163575, Blast_Score=1149, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=35.3463587921847, Blast_Score=325, Evalue=7e-90,
Organism=Escherichia coli, GI1790104, Length=476, Percent_Identity=35.0840336134454, Blast_Score=283, Evalue=3e-77,
Organism=Escherichia coli, GI1788716, Length=488, Percent_Identity=27.6639344262295, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=24.5009074410163, Blast_Score=138, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=506, Percent_Identity=24.901185770751, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17531299, Length=506, Percent_Identity=24.901185770751, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17542570, Length=540, Percent_Identity=23.3333333333333, Blast_Score=97, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=594, Percent_Identity=31.6498316498317, Blast_Score=269, Evalue=7e-73,
Organism=Saccharomyces cerevisiae, GI6320816, Length=565, Percent_Identity=23.716814159292, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=494, Percent_Identity=21.2550607287449, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321524, Length=499, Percent_Identity=21.2424849699399, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323073, Length=482, Percent_Identity=21.1618257261411, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19922626, Length=487, Percent_Identity=25.2566735112936, Blast_Score=138, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 64673; Mature: 64542

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRAVDAAMYVLEKEGVNTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEG
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
YSRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIE
HHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHCHHCCCEECHH
SIAKPVTKMAVTVREAALVPGVLQQAFHLMRSGRPGPVLIDLPYDVQVAEIEFDPELYSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECHHHCCC
LPAYKPIATRPQIEKALALLVQAERPVIVAGGGVINADAAALLQQFAEITHVPVIPTLMG
CCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCEECHHHHHHHHHHHHHCCCCCHHHHHC
WGCIPDDHELMAGMVGLQTAHRYGNATLLASDLVFGIGNRFANRHTGSVEKYTAGRKIIH
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCCCCHHCCCCCEEEE
IDIEPTQIGRVLCPDLGIVSDAKAALIQLVDIAQQMQKEGRLPRRESWVSECQARKRTLL
EECCHHHHCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEECCCCCCC
LGWTLPAALGVCAADPERNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
CCEEHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKA
HHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHCCCCCCEEEEECCCCCCHHHHHHHH
WMKAYRVPVVVEVILERVTNISMGSELDNVMEFEDVADSAIDAPTETCFMKYE
HHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHEEEECC
>Mature Secondary Structure 
AKMRAVDAAMYVLEKEGVNTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEG
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
YSRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIE
HHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHCHHCCCEECHH
SIAKPVTKMAVTVREAALVPGVLQQAFHLMRSGRPGPVLIDLPYDVQVAEIEFDPELYSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECHHHCCC
LPAYKPIATRPQIEKALALLVQAERPVIVAGGGVINADAAALLQQFAEITHVPVIPTLMG
CCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCEECHHHHHHHHHHHHHCCCCCHHHHHC
WGCIPDDHELMAGMVGLQTAHRYGNATLLASDLVFGIGNRFANRHTGSVEKYTAGRKIIH
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCCCCHHCCCCCEEEE
IDIEPTQIGRVLCPDLGIVSDAKAALIQLVDIAQQMQKEGRLPRRESWVSECQARKRTLL
EECCHHHHCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEECCCCCCC
LGWTLPAALGVCAADPERNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
CCEEHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKA
HHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHCCCCCCEEEEECCCCCCHHHHHHHH
WMKAYRVPVVVEVILERVTNISMGSELDNVMEFEDVADSAIDAPTETCFMKYE
HHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]