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The map label for this gene is hyi [H]

Identifier: 238893469

GI number: 238893469

Start: 1314552

End: 1315328

Strand: Direct

Name: hyi [H]

Synonym: KP1_1362

Alternate gene names: 238893469

Gene position: 1314552-1315328 (Clockwise)

Preceding gene: 238893468

Following gene: 238893470

Centisome position: 25.05

GC content: 47.75

Gene sequence:

>777_bases
ATGTTGCGCTTTTCTGCCAACCTTTCCATGTTATTTCAGGAATATGATTTTCTGGATCGCTTTGAGAAAGCGGCGGCCTG
TGGTTTTCGCGCTGTTGAATTTATGTTTCCCTATGACTATGCCCCCGAGCTATTAAAGCAAAAGCTGAGCGACAACCAGC
TGGAACATACTTTACACAATTTACCCGCTGGTGACTGGGCGGCAGGGGAACGAGGCATCGCTTGTATTCCAGGCCGCGAA
GTGGAGTTTCGCGAAGGTGTCGACGCCGCAATCCGCTACGCCAAAGCGCTGGGCAATCGTAAAATTAACTGTCTGGTCGG
CAAAACTCCGGTGGGTTATCGCGAAGAACATATTCGCTCAACGCTGGTGGCGAATTTACGTTTTGCCGCCGCCGCGCTGG
CCCAGGAAGGTATCCTGCTGCTAATTGAGCCGATCAATTACTTCGACATGCCGGGCTTTTATCTGAATACCACGCATCAG
GCGTTGGCGCTGATTGATGAAGTGGGTAATGATAATCTGAAAATTCAGTACGATATTTACCATATGCAGCGTATGGAGGG
GGAATTAACCCATACGATGCAAACTTACGCTAATCGTATCGGTCACTTACAGATCGCGGATAATCCGAATCGAGGTGAAC
CCGGGACCGGTGAAATTAATTATGAGTATCTTTTTAATTTCATCGATAAATCAAACTATGACGGTTGGGTAGGTTGTGAA
TATAAACCATTGACTACCACCGAAGCGGGACTGACATGGTTTAATCGCTACCGCTAA

Upstream 100 bases:

>100_bases
GAACTGGATAATGTCATGGAGTTCGAAGACGTTGCCGATAGCGCCATTGACGCACCAACCGAAACTTGTTTCATGAAATA
TGAATAAATGAGGTGACATT

Downstream 100 bases:

>100_bases
TTTTTAATTTATCGTTGGGCAGTATTTATTATTCCAGACGCAGGGGATTTATTTCCTGACAACCGGAAGGGATAGCTATT
GCCCAGATAAATCGAAATAT

Product: hydroxypyruvate isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLRFSANLSMLFQEYDFLDRFEKAAACGFRAVEFMFPYDYAPELLKQKLSDNQLEHTLHNLPAGDWAAGERGIACIPGRE
VEFREGVDAAIRYAKALGNRKINCLVGKTPVGYREEHIRSTLVANLRFAAAALAQEGILLLIEPINYFDMPGFYLNTTHQ
ALALIDEVGNDNLKIQYDIYHMQRMEGELTHTMQTYANRIGHLQIADNPNRGEPGTGEINYEYLFNFIDKSNYDGWVGCE
YKPLTTTEAGLTWFNRYR

Sequences:

>Translated_258_residues
MLRFSANLSMLFQEYDFLDRFEKAAACGFRAVEFMFPYDYAPELLKQKLSDNQLEHTLHNLPAGDWAAGERGIACIPGRE
VEFREGVDAAIRYAKALGNRKINCLVGKTPVGYREEHIRSTLVANLRFAAAALAQEGILLLIEPINYFDMPGFYLNTTHQ
ALALIDEVGNDNLKIQYDIYHMQRMEGELTHTMQTYANRIGHLQIADNPNRGEPGTGEINYEYLFNFIDKSNYDGWVGCE
YKPLTTTEAGLTWFNRYR
>Mature_258_residues
MLRFSANLSMLFQEYDFLDRFEKAAACGFRAVEFMFPYDYAPELLKQKLSDNQLEHTLHNLPAGDWAAGERGIACIPGRE
VEFREGVDAAIRYAKALGNRKINCLVGKTPVGYREEHIRSTLVANLRFAAAALAQEGILLLIEPINYFDMPGFYLNTTHQ
ALALIDEVGNDNLKIQYDIYHMQRMEGELTHTMQTYANRIGHLQIADNPNRGEPGTGEINYEYLFNFIDKSNYDGWVGCE
YKPLTTTEAGLTWFNRYR

Specific function: Catalyzes the reversible isomerization between hydroxypyruvate and 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3622

COG function: function code G; Hydroxypyruvate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hyi family [H]

Homologues:

Organism=Homo sapiens, GI300068962, Length=263, Percent_Identity=43.3460076045627, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI300068964, Length=244, Percent_Identity=43.0327868852459, Blast_Score=192, Evalue=3e-49,
Organism=Escherichia coli, GI1786718, Length=258, Percent_Identity=76.7441860465116, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1789095, Length=258, Percent_Identity=43.0232558139535, Blast_Score=232, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17552022, Length=240, Percent_Identity=38.75, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI17530883, Length=259, Percent_Identity=35.5212355212355, Blast_Score=166, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017643
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =5.3.1.22 [H]

Molecular weight: Translated: 29514; Mature: 29514

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFSANLSMLFQEYDFLDRFEKAAACGFRAVEFMFPYDYAPELLKQKLSDNQLEHTLHN
CEEECCHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCHHHHHHHHHCCHHHHHHHHH
LPAGDWAAGERGIACIPGREVEFREGVDAAIRYAKALGNRKINCLVGKTPVGYREEHIRS
CCCCCCCCCCCCEEECCCCCCHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
TLVANLRFAAAALAQEGILLLIEPINYFDMPGFYLNTTHQALALIDEVGNDNLKIQYDIY
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEECCHHHHHHHHHHHCCCCEEEEEEHH
HMQRMEGELTHTMQTYANRIGHLQIADNPNRGEPGTGEINYEYLFNFIDKSNYDGWVGCE
HHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCEEHHHHHHHHHCCCCCCEECCC
YKPLTTTEAGLTWFNRYR
CCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MLRFSANLSMLFQEYDFLDRFEKAAACGFRAVEFMFPYDYAPELLKQKLSDNQLEHTLHN
CEEECCHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCHHHHHHHHHCCHHHHHHHHH
LPAGDWAAGERGIACIPGREVEFREGVDAAIRYAKALGNRKINCLVGKTPVGYREEHIRS
CCCCCCCCCCCCEEECCCCCCHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
TLVANLRFAAAALAQEGILLLIEPINYFDMPGFYLNTTHQALALIDEVGNDNLKIQYDIY
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEECCHHHHHHHHHHHCCCCEEEEEEHH
HMQRMEGELTHTMQTYANRIGHLQIADNPNRGEPGTGEINYEYLFNFIDKSNYDGWVGCE
HHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCEEHHHHHHHHHCCCCCCEECCC
YKPLTTTEAGLTWFNRYR
CCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8440684; 10561547; 9278503 [H]