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The map label for this gene is tgt [H]

Identifier: 238893342

GI number: 238893342

Start: 1183587

End: 1184714

Strand: Direct

Name: tgt [H]

Synonym: KP1_1222

Alternate gene names: 238893342

Gene position: 1183587-1184714 (Clockwise)

Preceding gene: 238893341

Following gene: 238893343

Centisome position: 22.55

GC content: 55.94

Gene sequence:

>1128_bases
ATGAAATTTGAACTGGATACCACCGACGGTCGCGCCCGCCGCGGTCGCCTGGTGTTTGAGCGCGGCGTGGTGGAAACACC
GGCCTTTATGCCCGTTGGCACCTACGGCACCGTCAAAGGGATGACGCCGGAAGAGGTGGAAGCCACCGGCGCGCAGATTA
TTCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGTCAGGAGATCATGAAGCTGCACGGCGATCTGCACGATTTTATG
CAGTGGAAAGGACCGATCCTGACCGACTCCGGCGGTTTCCAGGTGTTCAGCCTTGGCGATATCCGTAAGATCACCGAGCA
GGGCGTCCACTTCCGCAATCCGATCAACGGCGATCCGATTTTCCTCGATCCGGAAAAATCGATGGAGATTCAGTACGATC
TCGGCTCTGACATCGTGATGATCTTCGACGAATGTACGCCGTACCCGGCGGACTGGGATTACGCCAAGCGCTCAATGGAG
ATGTCTCTGCGCTGGGCGAAGCGCAGCCGCGACCGTTTCGATAGCCTTGGCAATAAAAATGCGCTGTTCGGCATTATTCA
GGGTAGCGTTTACGAAGATTTACGCGATATCTCGGTGAAAGGTCTGGTAGAGATTGGCTTTGATGGCTACGCTGTCGGCG
GTTTGGCGGTTGGTGAGCCGAAGGAAGACATGCACCGTATTCTGGAGCACGTCTGCCCGCAGATCCCGGCCGATAAACCA
CGATACCTGATGGGCGTCGGGAAGCCGGAAGATCTGGTGGAAGGGGTACGTCGCGGTATCGATATGTTCGACTGCGTCAT
GCCGACGCGCAACGCGCGTAACGGCCACCTGTTCGTCACCGACGGCGTGGTGAAAATCCGCAACGCGAAGCACAAAAGCG
ATACCGCGCCGCTGGACGCCGAGTGTGATTGCTATACATGTCGCAATTATTCACGCGCTTACTTGCATCACCTCGACCGT
TGCAACGAAATATTGGGCGCGCGTCTCAATACCATTCATAACCTGCGCTACTATCAGCGTTTAATGGCTGGTTTACGCAA
GGCTATCGAAGAGGGTAAATTAGAGAGCTTCGTGACCGATTTTTACCAACGTCAGGGGCGCACCGTGCCTCCTTTGAACG
TTGATTAA

Upstream 100 bases:

>100_bases
GTACGCCGGCTTGTAGACGCCATAACGCATTTTTTAGCTACGGAATCATTCCGTGGCGAGTATGAAAACGTCGGACTGTT
TTTCTGACGCAGGAGCAAGC

Downstream 100 bases:

>100_bases
TTTTAATAATGAGGGAATTTCAATGAGCTTTTTTATTTCTGATGCGGTAGCGGCAACGGGTGCACCGGCGCAGGGCAGCC
CGATGTCTCTGATTCTGATG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKFELDTTDGRARRGRLVFERGVVETPAFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFM
QWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
MSLRWAKRSRDRFDSLGNKNALFGIIQGSVYEDLRDISVKGLVEIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPADKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKSDTAPLDAECDCYTCRNYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGRTVPPLNVD

Sequences:

>Translated_375_residues
MKFELDTTDGRARRGRLVFERGVVETPAFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFM
QWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
MSLRWAKRSRDRFDSLGNKNALFGIIQGSVYEDLRDISVKGLVEIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPADKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKSDTAPLDAECDCYTCRNYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGRTVPPLNVD
>Mature_375_residues
MKFELDTTDGRARRGRLVFERGVVETPAFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFM
QWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
MSLRWAKRSRDRFDSLGNKNALFGIIQGSVYEDLRDISVKGLVEIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPADKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKSDTAPLDAECDCYTCRNYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGRTVPPLNVD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=364, Percent_Identity=42.8571428571429, Blast_Score=300, Evalue=1e-81,
Organism=Escherichia coli, GI1786607, Length=375, Percent_Identity=97.6, Blast_Score=761, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=41.1602209944751, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI25146502, Length=166, Percent_Identity=31.9277108433735, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19920510, Length=369, Percent_Identity=43.6314363143631, Blast_Score=308, Evalue=5e-84,
Organism=Drosophila melanogaster, GI21355311, Length=172, Percent_Identity=31.3953488372093, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42568; Mature: 42568

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFELDTTDGRARRGRLVFERGVVETPAFMPVGTYGTVKGMTPEEVEATGAQIILGNTFH
CEEECCCCCCCHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPI
EEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCCE
FLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRDRFDSLGNKN
EECCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ALFGIIQGSVYEDLRDISVKGLVEIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPADKP
EEEEEECCHHHHHHHHCCHHEEEEECCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKSDTAPLDA
CEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCC
ECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTD
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
FYQRQGRTVPPLNVD
HHHHCCCCCCCCCCC
>Mature Secondary Structure
MKFELDTTDGRARRGRLVFERGVVETPAFMPVGTYGTVKGMTPEEVEATGAQIILGNTFH
CEEECCCCCCCHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPI
EEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCCE
FLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRDRFDSLGNKN
EECCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ALFGIIQGSVYEDLRDISVKGLVEIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPADKP
EEEEEECCHHHHHHHHCCHHEEEEECCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKSDTAPLDA
CEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCC
ECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTD
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
FYQRQGRTVPPLNVD
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA