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The map label for this gene is pulJ [H]

Identifier: 238893133

GI number: 238893133

Start: 963891

End: 964487

Strand: Reverse

Name: pulJ [H]

Synonym: KP1_0983

Alternate gene names: 238893133

Gene position: 964487-963891 (Counterclockwise)

Preceding gene: 238893134

Following gene: 238893132

Centisome position: 18.38

GC content: 64.82

Gene sequence:

>597_bases
ATGATAACTAGGATGCGCGGTTTCACCCTGATTGAAACGCTGCTGGCGCTGGCTATCCTCGCCGTCCTCAGCGCCGCCGC
CGTGATGGTGCTGCAGAACGTCATCCGCGCCGATGGCCTGACCCGCGAGAAAAGCCAGCAGATCGCGGCCCTCCAGCGCG
CCTTTCGCCAGATAGCCGACGATGTCACCCACATCATTCCCCGTCGCGCCAGAAACAGCGACACGTTTTTCTTCGCCGGA
CGTTTCCAGTTGCAGAGCGACGACTGGGGACTGGCCTTCAGCCGCAGCGGCTGGCCAAACCCGCTGGGGATCCTCCCGCG
CTCGGAGATCCAGAACGTCAGCTACCGTCTGCGCCAGCAGCAGCTTGAGCGTCTGAGCTTCGATCAACAGGATCCGCTGA
CCGGCAGCCAGCCGACGGTCCGGGTGCTGCTGCGCGAGGTAACCGCGTTTCGCCTGCGGTTTTACGCCGACGGGCGCTGG
CAGGAAACCTGGGACCGCCCGCAGAGGCTGCCGCAGGGGCTGGAGATCACCCTGACGCTGGCGAACAGCGGGGAGATAAC
CCGCCTGTTTTTACTCACCCCGGGAGGCGGCCAGTGA

Upstream 100 bases:

>100_bases
AAACCGCGCTGCCGCAGCTGCGGGCGCTGGACGTGGAGGTCCGGCGGCAAAAAAGCGATCCCGCGCCGCTGGCCACCCTG
CGCACCTGGGTGACGCCGCC

Downstream 100 bases:

>100_bases
TAAATCGGCAACGCGGCGTCGCGCTCCTGATGGTGCTTTTCATCCTCGCCCTGATGATGATCCTCGCCAGCGCCATGACC
GAGCGCACCGCGGTCATGTA

Product: pullulanase-specific type II secretion system component J

Products: NA

Alternate protein names: Pullulanase secretion protein pulJ [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MITRMRGFTLIETLLALAILAVLSAAAVMVLQNVIRADGLTREKSQQIAALQRAFRQIADDVTHIIPRRARNSDTFFFAG
RFQLQSDDWGLAFSRSGWPNPLGILPRSEIQNVSYRLRQQQLERLSFDQQDPLTGSQPTVRVLLREVTAFRLRFYADGRW
QETWDRPQRLPQGLEITLTLANSGEITRLFLLTPGGGQ

Sequences:

>Translated_198_residues
MITRMRGFTLIETLLALAILAVLSAAAVMVLQNVIRADGLTREKSQQIAALQRAFRQIADDVTHIIPRRARNSDTFFFAG
RFQLQSDDWGLAFSRSGWPNPLGILPRSEIQNVSYRLRQQQLERLSFDQQDPLTGSQPTVRVLLREVTAFRLRFYADGRW
QETWDRPQRLPQGLEITLTLANSGEITRLFLLTPGGGQ
>Mature_198_residues
MITRMRGFTLIETLLALAILAVLSAAAVMVLQNVIRADGLTREKSQQIAALQRAFRQIADDVTHIIPRRARNSDTFFFAG
RFQLQSDDWGLAFSRSGWPNPLGILPRSEIQNVSYRLRQQQLERLSFDQQDPLTGSQPTVRVLLREVTAFRLRFYADGRW
QETWDRPQRLPQGLEITLTLANSGEITRLFLLTPGGGQ

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG4795

COG function: function code U; Type II secretory pathway, component PulJ

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP J family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010055
- InterPro:   IPR012902
- InterPro:   IPR001120 [H]

Pfam domain/function: PF07963 N_methyl [H]

EC number: NA

Molecular weight: Translated: 22523; Mature: 22523

Theoretical pI: Translated: 11.53; Mature: 11.53

Prosite motif: PS00409 PROKAR_NTER_METHYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRMRGFTLIETLLALAILAVLSAAAVMVLQNVIRADGLTREKSQQIAALQRAFRQIAD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
DVTHIIPRRARNSDTFFFAGRFQLQSDDWGLAFSRSGWPNPLGILPRSEIQNVSYRLRQQ
HHHHHHHHCCCCCCEEEEEEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
QLERLSFDQQDPLTGSQPTVRVLLREVTAFRLRFYADGRWQETWDRPQRLPQGLEITLTL
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHCCCCCEEEEEE
ANSGEITRLFLLTPGGGQ
ECCCCEEEEEEECCCCCC
>Mature Secondary Structure
MITRMRGFTLIETLLALAILAVLSAAAVMVLQNVIRADGLTREKSQQIAALQRAFRQIAD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
DVTHIIPRRARNSDTFFFAGRFQLQSDDWGLAFSRSGWPNPLGILPRSEIQNVSYRLRQQ
HHHHHHHHCCCCCCEEEEEEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
QLERLSFDQQDPLTGSQPTVRVLLREVTAFRLRFYADGRWQETWDRPQRLPQGLEITLTL
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHCCCCCEEEEEE
ANSGEITRLFLLTPGGGQ
ECCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2129543 [H]