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The map label for this gene is ligT [H]

Identifier: 238893126

GI number: 238893126

Start: 956631

End: 957167

Strand: Reverse

Name: ligT [H]

Synonym: KP1_0975

Alternate gene names: 238893126

Gene position: 957167-956631 (Counterclockwise)

Preceding gene: 238893128

Following gene: 238893125

Centisome position: 18.24

GC content: 64.25

Gene sequence:

>537_bases
ATGTCTGAGCCGAAAAGGCTGTTTTTTGCGCTTGAACTGCCGTCTGCCGTCCAGAAGCAAATTGTCCAGTGGCGGGCAAC
GCACTTCCCCGAAGATGCCGGCAGGCCCGTCGCGGCGGATAATCTTCATCTGACGCTGGCGTTTCTCGGCGAGGTGAGCG
CGGAAAAACAGCGGGCGCTGGCCGCTCTCGCTGGCCGGCTGCGCCAGCCGGGCTTTACCCTGACGCTGGACGATGCCGGA
CAGTGGCTACGTTCGCGAGTGGTATGGCTCGGAACCCGCCAGCCGCCGCGCGGCCTGCTGCAGCTCGCCAGCATGTTGCG
CGCTCAGGCGGCGCGCAGCGGCTGCTATCAGAGCCCGCAGCCGTTTCACCCGCATATTACCCTATGGCGCGATGCTCGCC
GGGCGGTGCCGATCCCGGCCCCCGGCTTTCGCTGGACGTTTCCGGTGAACGAGTTCGTGCTCTATGAGTCCAGCTTTAGC
CGTGGGCGCACGCGCTACACGGCGCTGGAACGTTATCCGTTTGATAAGGAAAGTTGA

Upstream 100 bases:

>100_bases
GGACAGCGGCAACCGGCAATGAGGACACAAAAGGCTCCGGGGGTTAACATTCTGGGGTTCGCATTGTAGCATCCCCCTAA
TTCATTACCGAGTCTCTATC

Downstream 100 bases:

>100_bases
TGCAGTTTGATCCGCCGTTGCAGCCCGCCATTTTGCTTAAACGTTACAAACGATTTCTTGCTGACGTGGTCACCCCCGAT
GGCCGCGAGCTGACGCTCCA

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MSEPKRLFFALELPSAVQKQIVQWRATHFPEDAGRPVAADNLHLTLAFLGEVSAEKQRALAALAGRLRQPGFTLTLDDAG
QWLRSRVVWLGTRQPPRGLLQLASMLRAQAARSGCYQSPQPFHPHITLWRDARRAVPIPAPGFRWTFPVNEFVLYESSFS
RGRTRYTALERYPFDKES

Sequences:

>Translated_178_residues
MSEPKRLFFALELPSAVQKQIVQWRATHFPEDAGRPVAADNLHLTLAFLGEVSAEKQRALAALAGRLRQPGFTLTLDDAG
QWLRSRVVWLGTRQPPRGLLQLASMLRAQAARSGCYQSPQPFHPHITLWRDARRAVPIPAPGFRWTFPVNEFVLYESSFS
RGRTRYTALERYPFDKES
>Mature_177_residues
SEPKRLFFALELPSAVQKQIVQWRATHFPEDAGRPVAADNLHLTLAFLGEVSAEKQRALAALAGRLRQPGFTLTLDDAGQ
WLRSRVVWLGTRQPPRGLLQLASMLRAQAARSGCYQSPQPFHPHITLWRDARRAVPIPAPGFRWTFPVNEFVLYESSFSR
GRTRYTALERYPFDKES

Specific function: Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage [H]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081692, Length=172, Percent_Identity=76.7441860465116, Blast_Score=281, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 20257; Mature: 20126

Theoretical pI: Translated: 11.09; Mature: 11.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPKRLFFALELPSAVQKQIVQWRATHFPEDAGRPVAADNLHLTLAFLGEVSAEKQRAL
CCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEEHHCHHHHHHHHH
AALAGRLRQPGFTLTLDDAGQWLRSRVVWLGTRQPPRGLLQLASMLRAQAARSGCYQSPQ
HHHHHHHCCCCCEEEECHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
PFHPHITLWRDARRAVPIPAPGFRWTFPVNEFVLYESSFSRGRTRYTALERYPFDKES
CCCCCEEEECCCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SEPKRLFFALELPSAVQKQIVQWRATHFPEDAGRPVAADNLHLTLAFLGEVSAEKQRAL
CCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEEHHCHHHHHHHHH
AALAGRLRQPGFTLTLDDAGQWLRSRVVWLGTRQPPRGLLQLASMLRAQAARSGCYQSPQ
HHHHHHHCCCCCEEEECHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
PFHPHITLWRDARRAVPIPAPGFRWTFPVNEFVLYESSFSRGRTRYTALERYPFDKES
CCCCCEEEECCCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 2013578; 8940112 [H]