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The map label for this gene is pulO [H]

Identifier: 238893128

GI number: 238893128

Start: 959717

End: 960517

Strand: Reverse

Name: pulO [H]

Synonym: KP1_0977

Alternate gene names: 238893128

Gene position: 960517-959717 (Counterclockwise)

Preceding gene: 238893129

Following gene: 238893126

Centisome position: 18.3

GC content: 66.17

Gene sequence:

>801_bases
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Upstream 100 bases:

>100_bases
CGCCGCTGCTGGCGCTGCTGGTCACGCGCCCGACAGCGAATAACGCCCCGGGCCCGACGCCGTGGCAGCTGCAGGGAAAA
TGGTTACCACAGGAGCAAAA

Downstream 100 bases:

>100_bases
CGTTTGCGAACCGTCTGGCCGGGCAGGATGCCGCCCGGCAGCGGCGGCTACGAGTATTTTTTGCTGCGGCGGGTCGGGGC
GGCGTTGGCCGGATCGTCCG

Product: pullulanase-specific type II secretion system type 4 prepilin peptidase

Products: NA

Alternate protein names: Pullulanase secretion protein pulO; Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Sequences:

>Translated_266_residues
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV
>Mature_265_residues
TTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWLG
RRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLNE
VYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQS
LQQPLAFGPWLALAGGGIFLWQQMV

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Required for the translocation of pullulanase [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=244, Percent_Identity=40.9836065573771, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI1789732, Length=238, Percent_Identity=42.0168067226891, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 29389; Mature: 29258

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCH
QCRQPIAWRDNIPLLSFLWLGRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLA
HHCCCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLNEVYIALPDAVAGAMAGYLAL
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHEEECCHHHHHHHHHHHHH
WSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
HHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SLQQPLAFGPWLALAGGGIFLWQQMV
HHHCCHHHCCHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
TTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCH
QCRQPIAWRDNIPLLSFLWLGRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLA
HHCCCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLNEVYIALPDAVAGAMAGYLAL
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHEEECCHHHHHHHHHHHHH
WSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
HHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SLQQPLAFGPWLALAGGGIFLWQQMV
HHHCCHHHCCHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2162463 [H]