Click here to switch to the map view.

The map label for this gene is imp [H]

Identifier: 238893027

GI number: 238893027

Start: 840493

End: 842841

Strand: Reverse

Name: imp [H]

Synonym: KP1_0864

Alternate gene names: 238893027

Gene position: 842841-840493 (Counterclockwise)

Preceding gene: 238893029

Following gene: 238893026

Centisome position: 16.06

GC content: 55.68

Gene sequence:

>2349_bases
ATGAAAAAACGTATTCCCAGCCTCCTGGCTACGATGATTGCCAGCGCCTTGTATAGCCAACAAGGCCTCGCTGCCGATCT
CGCAACGCAATGTATGCTTGGCGTGCCAAGCTATGATCGTCCGCTCGTGGAAGGTCGTCCTGGCGATCTGCCGGTGACGA
TTAACGCCGATCATGCGAAGGGCAACTACCCGGACAACGCCGTCTTTACCGGCAACGTCGATATTAACCAGGGGAATAGT
CGCCTCCGCGCCGACGAAGTTCAGCTGCACCAGCAGCAGGCCGCGGGCCAGGCGCAGCCGGTGCGCACGGTGGACGCGCT
GGGCAACGTGCATTACGACGATAACCAGGTGATCCTCAAAGGGCCAAAAGCCTGGTCGAATCTGAATACCAAAGATACCA
ACGTCTGGCAGGGCGATTATCAAATGGTCGGACGCCAGGGACGCGGCACCGCCGACCTGATGAAACAGCGCGGTGAAAAC
CGCTATACCATTCTCGAAAACGGCAGCTTTACCTCCTGTCTGCCGGGCTCCGACACCTGGAGCGTCGTCGGCAGCGAAGT
TATCCACGATCGCGAAGAACAGGTTGCCGAGATCTGGAACGCCCGCTTCAAGCTTGGCTCTGTGCCGATTTTCTATAGCC
CCTACCTGCAGCTGCCGGTGGGCGATAAGCGTCGTTCAGGCTTCCTGATCCCGAACGCGAAATACAGCACCAAAAACGGC
GTGGAATTCTCCCTGCCGTACTACTGGAACATCGCGCCAAACTTCGATGCCACTATTACTCCGCACTATATGAACAAACG
CGGCGGCGTGATGTGGGAAAACGAGTTCCGCTATCTGACCCAGCTCGGCAGCGGCTTAACTGAATTCGACTACCTGCCGT
CGGATAAAGTCTACGAAGACGACCACTCGAGCGACAGCAACAGCCGCCGCTGGCTGTTCTACTGGAACCACTCAGGGGTT
ATCGATCAGGTATGGCGTCTGAACGCTGACTACACCAAGGTCAGCGATCCTGACTACTTCAACGACTTCAGCTCGAAATA
TGGTTCCAGTACCGACGGCTATGCGACGCAGAAATTCAGCGCCGGTTACGTCAACCAGAACTTTGACGCCACGGTATCGA
CCAAACAGTTCCAGGTCTTTGACCGCGAATCGAGCAACTCCTATTCGGCTGAGCCGCAGCTCGACGTCAACTACTACCAG
AATGATGTCGGTCCGTTCGATACCCATCTCTATGGACAGGTTGCCCATTTTGTTAACTCGAATAACAACATGCCGGAAGC
GACCCGCGTTCACTTCGAACCGACGATCAACCTGCCGCTGTCCAACGATTGGGGCAGTCTGAATACCGAAGCCAAGCTGC
TGGCGACTCACTACCAGCAGAGCAACCTCGATAAGTACAATGCCGCCAACGGCACTGACTATAAAGAGTCCGTCAGCCGC
GTAATGCCGCAGTTTAAAGTCGACGGCAAAATGGTCTTTGAACGCGACCTGCAGGAGGGATTCACCCAAACGCTGGAACC
GCGCGTGCAGTATCTGTACGTGCCGTACCGCGATCAGAGTGAAATCGGCAACTACGACTCCACGCTGTTGCAGTCGGATT
ACACCGGTCTGTTCCGCGACCGTACCTATAGCGGTCTGGACCGCATCGCGTCGGCTAATCAGGTCACCACCGGCCTCACC
TCGCGCGTGTACGATGCCGCCGCGGTGGAACGTTTTAATATTTCCGTCGGTCAAATCTACTATTTCACCGAGTCACGCAC
CGGTGATGACAACATCAACTGGGAGAACAACGATACCACGGGTTCACTGGTCTGGGCCGGCGATACCTACTGGCGCATTG
CCGATGAATGGGGTCTGCGCGGGGGGATCCAGTACGATACGCGTCTGGATAACGTTGCCACTGGTAACGGCACCATTGAA
TACCGTCGCGATGAGAACCGCTTAGTCCAGCTTAACTATCGTTACGCCAGCCCGGAATATATTCAGGCCACGCTGCCGTC
ATATTCCACCGCGGCACAATATAAACAGGGTATTTCGCAGGTGGGGATGACCGCCAGTTGGCCGATTGTCGATCGCTGGT
CCGTGGTCGGGGCCTACTACTACGATACTAATACCCGGAAAGCAGCAAACCAGATGTTGGGTGTGCAGTATAACTCCTGC
TGCTACGCCATCCGTCTTGGCTACGAACGTAAAGTCAACGGCTGGAACAGCAACGACAACGGCGGCGAGAGCAAATACGA
TAACACCTTCGGAATCAATATCGAATTGCGCGGTCTGAGCTCTAACTACGGCCTCGGCACCCAGCAGATGCTGCGCTCGA
ACATTTTACCGTACCAGAGCTCCCTGTGA

Upstream 100 bases:

>100_bases
AAACGGGCAGGATTAACACTAGCGTGATAATGTTGAGTAAGTTAGGCTCTGACCGTTTGCAAGCCGCTGTCACAAGATGA
TGGAACAATAAATACAACGT

Downstream 100 bases:

>100_bases
TGCGGCTGGTTTACAACATCATCCGCATTTGCGGTTAATCGAAATGGAAAAAGTATGAAGAACTGGAAAACGCTGCTTCT
CGGTATCGCCATGATCGCGA

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 782; Mature: 782

Protein sequence:

>782_residues
MKKRIPSLLATMIASALYSQQGLAADLATQCMLGVPSYDRPLVEGRPGDLPVTINADHAKGNYPDNAVFTGNVDINQGNS
RLRADEVQLHQQQAAGQAQPVRTVDALGNVHYDDNQVILKGPKAWSNLNTKDTNVWQGDYQMVGRQGRGTADLMKQRGEN
RYTILENGSFTSCLPGSDTWSVVGSEVIHDREEQVAEIWNARFKLGSVPIFYSPYLQLPVGDKRRSGFLIPNAKYSTKNG
VEFSLPYYWNIAPNFDATITPHYMNKRGGVMWENEFRYLTQLGSGLTEFDYLPSDKVYEDDHSSDSNSRRWLFYWNHSGV
IDQVWRLNADYTKVSDPDYFNDFSSKYGSSTDGYATQKFSAGYVNQNFDATVSTKQFQVFDRESSNSYSAEPQLDVNYYQ
NDVGPFDTHLYGQVAHFVNSNNNMPEATRVHFEPTINLPLSNDWGSLNTEAKLLATHYQQSNLDKYNAANGTDYKESVSR
VMPQFKVDGKMVFERDLQEGFTQTLEPRVQYLYVPYRDQSEIGNYDSTLLQSDYTGLFRDRTYSGLDRIASANQVTTGLT
SRVYDAAAVERFNISVGQIYYFTESRTGDDNINWENNDTTGSLVWAGDTYWRIADEWGLRGGIQYDTRLDNVATGNGTIE
YRRDENRLVQLNYRYASPEYIQATLPSYSTAAQYKQGISQVGMTASWPIVDRWSVVGAYYYDTNTRKAANQMLGVQYNSC
CYAIRLGYERKVNGWNSNDNGGESKYDNTFGINIELRGLSSNYGLGTQQMLRSNILPYQSSL

Sequences:

>Translated_782_residues
MKKRIPSLLATMIASALYSQQGLAADLATQCMLGVPSYDRPLVEGRPGDLPVTINADHAKGNYPDNAVFTGNVDINQGNS
RLRADEVQLHQQQAAGQAQPVRTVDALGNVHYDDNQVILKGPKAWSNLNTKDTNVWQGDYQMVGRQGRGTADLMKQRGEN
RYTILENGSFTSCLPGSDTWSVVGSEVIHDREEQVAEIWNARFKLGSVPIFYSPYLQLPVGDKRRSGFLIPNAKYSTKNG
VEFSLPYYWNIAPNFDATITPHYMNKRGGVMWENEFRYLTQLGSGLTEFDYLPSDKVYEDDHSSDSNSRRWLFYWNHSGV
IDQVWRLNADYTKVSDPDYFNDFSSKYGSSTDGYATQKFSAGYVNQNFDATVSTKQFQVFDRESSNSYSAEPQLDVNYYQ
NDVGPFDTHLYGQVAHFVNSNNNMPEATRVHFEPTINLPLSNDWGSLNTEAKLLATHYQQSNLDKYNAANGTDYKESVSR
VMPQFKVDGKMVFERDLQEGFTQTLEPRVQYLYVPYRDQSEIGNYDSTLLQSDYTGLFRDRTYSGLDRIASANQVTTGLT
SRVYDAAAVERFNISVGQIYYFTESRTGDDNINWENNDTTGSLVWAGDTYWRIADEWGLRGGIQYDTRLDNVATGNGTIE
YRRDENRLVQLNYRYASPEYIQATLPSYSTAAQYKQGISQVGMTASWPIVDRWSVVGAYYYDTNTRKAANQMLGVQYNSC
CYAIRLGYERKVNGWNSNDNGGESKYDNTFGINIELRGLSSNYGLGTQQMLRSNILPYQSSL
>Mature_782_residues
MKKRIPSLLATMIASALYSQQGLAADLATQCMLGVPSYDRPLVEGRPGDLPVTINADHAKGNYPDNAVFTGNVDINQGNS
RLRADEVQLHQQQAAGQAQPVRTVDALGNVHYDDNQVILKGPKAWSNLNTKDTNVWQGDYQMVGRQGRGTADLMKQRGEN
RYTILENGSFTSCLPGSDTWSVVGSEVIHDREEQVAEIWNARFKLGSVPIFYSPYLQLPVGDKRRSGFLIPNAKYSTKNG
VEFSLPYYWNIAPNFDATITPHYMNKRGGVMWENEFRYLTQLGSGLTEFDYLPSDKVYEDDHSSDSNSRRWLFYWNHSGV
IDQVWRLNADYTKVSDPDYFNDFSSKYGSSTDGYATQKFSAGYVNQNFDATVSTKQFQVFDRESSNSYSAEPQLDVNYYQ
NDVGPFDTHLYGQVAHFVNSNNNMPEATRVHFEPTINLPLSNDWGSLNTEAKLLATHYQQSNLDKYNAANGTDYKESVSR
VMPQFKVDGKMVFERDLQEGFTQTLEPRVQYLYVPYRDQSEIGNYDSTLLQSDYTGLFRDRTYSGLDRIASANQVTTGLT
SRVYDAAAVERFNISVGQIYYFTESRTGDDNINWENNDTTGSLVWAGDTYWRIADEWGLRGGIQYDTRLDNVATGNGTIE
YRRDENRLVQLNYRYASPEYIQATLPSYSTAAQYKQGISQVGMTASWPIVDRWSVVGAYYYDTNTRKAANQMLGVQYNSC
CYAIRLGYERKVNGWNSNDNGGESKYDNTFGINIELRGLSSNYGLGTQQMLRSNILPYQSSL

Specific function: Together with lptE, is involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=786, Percent_Identity=82.3155216284987, Blast_Score=1364, Evalue=0.0,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 88547; Mature: 88547

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRIPSLLATMIASALYSQQGLAADLATQCMLGVPSYDRPLVEGRPGDLPVTINADHAK
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCC
GNYPDNAVFTGNVDINQGNSRLRADEVQLHQQQAAGQAQPVRTVDALGNVHYDDNQVILK
CCCCCCCEEEEEEEECCCCCCEEHHHHHHHHHHHCCCCCCCHHHHHHCCCEECCCEEEEE
GPKAWSNLNTKDTNVWQGDYQMVGRQGRGTADLMKQRGENRYTILENGSFTSCLPGSDTW
CCCCCCCCCCCCCEEECCHHHHHCCCCCCHHHHHHHCCCCCEEEEECCCCCEECCCCCCH
SVVGSEVIHDREEQVAEIWNARFKLGSVPIFYSPYLQLPVGDKRRSGFLIPNAKYSTKNG
HHHHHHHHHCHHHHHHHHHHHHEEECCCCEEECCEEECCCCCCCCCCEECCCCCCCCCCC
VEFSLPYYWNIAPNFDATITPHYMNKRGGVMWENEFRYLTQLGSGLTEFDYLPSDKVYED
CEEECCEEEECCCCCCCEECCHHHCCCCCEEECHHHHHHHHHCCCCCHHCCCCCCCCCCC
DHSSDSNSRRWLFYWNHSGVIDQVWRLNADYTKVSDPDYFNDFSSKYGSSTDGYATQKFS
CCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEC
AGYVNQNFDATVSTKQFQVFDRESSNSYSAEPQLDVNYYQNDVGPFDTHLYGQVAHFVNS
CCCCCCCCCCEEECEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCC
NNNMPEATRVHFEPTINLPLSNDWGSLNTEAKLLATHYQQSNLDKYNAANGTDYKESVSR
CCCCCCCEEEEECEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
VMPQFKVDGKMVFERDLQEGFTQTLEPRVQYLYVPYRDQSEIGNYDSTLLQSDYTGLFRD
HCCCEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHCCCCHHHHHHCCCCHHHHC
RTYSGLDRIASANQVTTGLTSRVYDAAAVERFNISVGQIYYFTESRTGDDNINWENNDTT
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCEEEEEEEEECCCCCCCCCCCCCCCCC
GSLVWAGDTYWRIADEWGLRGGIQYDTRLDNVATGNGTIEYRRDENRLVQLNYRYASPEY
EEEEECCCCEEEEEHHCCCCCCCEECCCCCCEECCCCEEEEEECCCEEEEEEEEECCCCE
IQATLPSYSTAAQYKQGISQVGMTASWPIVDRWSVVGAYYYDTNTRKAANQMLGVQYNSC
EEEECCCCHHHHHHHHHHHHCCCCCCCCCEECCEEEEEEEEECCCHHHHHHHHCCEECCE
CYAIRLGYERKVNGWNSNDNGGESKYDNTFGINIELRGLSSNYGLGTQQMLRSNILPYQS
EEEEEECCEEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHCCCCCCC
SL
CC
>Mature Secondary Structure
MKKRIPSLLATMIASALYSQQGLAADLATQCMLGVPSYDRPLVEGRPGDLPVTINADHAK
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCC
GNYPDNAVFTGNVDINQGNSRLRADEVQLHQQQAAGQAQPVRTVDALGNVHYDDNQVILK
CCCCCCCEEEEEEEECCCCCCEEHHHHHHHHHHHCCCCCCCHHHHHHCCCEECCCEEEEE
GPKAWSNLNTKDTNVWQGDYQMVGRQGRGTADLMKQRGENRYTILENGSFTSCLPGSDTW
CCCCCCCCCCCCCEEECCHHHHHCCCCCCHHHHHHHCCCCCEEEEECCCCCEECCCCCCH
SVVGSEVIHDREEQVAEIWNARFKLGSVPIFYSPYLQLPVGDKRRSGFLIPNAKYSTKNG
HHHHHHHHHCHHHHHHHHHHHHEEECCCCEEECCEEECCCCCCCCCCEECCCCCCCCCCC
VEFSLPYYWNIAPNFDATITPHYMNKRGGVMWENEFRYLTQLGSGLTEFDYLPSDKVYED
CEEECCEEEECCCCCCCEECCHHHCCCCCEEECHHHHHHHHHCCCCCHHCCCCCCCCCCC
DHSSDSNSRRWLFYWNHSGVIDQVWRLNADYTKVSDPDYFNDFSSKYGSSTDGYATQKFS
CCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEC
AGYVNQNFDATVSTKQFQVFDRESSNSYSAEPQLDVNYYQNDVGPFDTHLYGQVAHFVNS
CCCCCCCCCCEEECEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCC
NNNMPEATRVHFEPTINLPLSNDWGSLNTEAKLLATHYQQSNLDKYNAANGTDYKESVSR
CCCCCCCEEEEECEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
VMPQFKVDGKMVFERDLQEGFTQTLEPRVQYLYVPYRDQSEIGNYDSTLLQSDYTGLFRD
HCCCEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHCCCCHHHHHHCCCCHHHHC
RTYSGLDRIASANQVTTGLTSRVYDAAAVERFNISVGQIYYFTESRTGDDNINWENNDTT
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCEEEEEEEEECCCCCCCCCCCCCCCCC
GSLVWAGDTYWRIADEWGLRGGIQYDTRLDNVATGNGTIEYRRDENRLVQLNYRYASPEY
EEEEECCCCEEEEEHHCCCCCCCEECCCCCCEECCCCEEEEEECCCEEEEEEEEECCCCE
IQATLPSYSTAAQYKQGISQVGMTASWPIVDRWSVVGAYYYDTNTRKAANQMLGVQYNSC
EEEECCCCHHHHHHHHHHHHCCCCCCCCCEECCEEEEEEEEECCCHHHHHHHHCCEECCE
CYAIRLGYERKVNGWNSNDNGGESKYDNTFGINIELRGLSSNYGLGTQQMLRSNILPYQS
EEEEEECCEEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHCCCCCCC
SL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA