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The map label for this gene is opuE [H]

Identifier: 238892928

GI number: 238892928

Start: 736788

End: 738281

Strand: Direct

Name: opuE [H]

Synonym: KP1_0753

Alternate gene names: 238892928

Gene position: 736788-738281 (Clockwise)

Preceding gene: 238892927

Following gene: 238892929

Centisome position: 14.04

GC content: 57.63

Gene sequence:

>1494_bases
ATGAATAATATGTCATTTATGATTTGGTTTTCCGTTTATGCCTGCGCGATGATTACCTTAGGCTGGTACGTTTCGCGCAA
ACAGAAAACCGGCGAAGACTTTTTACTTGGCGGTCGTTCGCTGCCGATGATCCTCACTCTCGGTTCGACGGTCGGCACCA
TGGTGGGGACCGGCTCCAGCGTCGGCGCCGTCGGCTTTGGCTACAGCAACGGCTGGGCCGGGATGCTTTACGGCCTCGGC
GGCGCGGTGGGGATTCTGCTGGTGGCATGGTTATTCGCGCCGGTACGTAAACTTCGCTTCATGACCATGAGTGAAGAAAT
GTCGTATTACACTGGCGGCAGTAAAATTATAAAAAACCTCGTCGCGATACTTATTTTTATTGCCTCTATCGGCTGGCTGG
GGGCGCATATATTAGGCGGCGGATTATATCTGGCGTGGGCCAGCGGCATTGATATTAATGTCGCGAAAATTATTATCGCG
CTGGCTTTTGTGGTTTATGTCGGTATCGGCGGCTATTCCGCAGTCGTGTGGATCGATACCATTCAATCGATCGTGCTGTT
CGTGGGCTTTATTTTAATGGCGATACTGGCGGTTCATCACGTTGGCGGCTGGAGCCATATTCAGCAGGCCGTCGATCCGG
CGGCGCAAAGCCTGTTCGCGGTCGATAAATTAGGCGTGCTGCCGGCGCTATCATTGGCGATGGTGATTGGCGTCGGCGTG
CTGGCGACGCCTTCTTATCGCCAGCGTATTTACTCGGCGAAGACCGTCTCCTCGGTGCGCCAGTCCTTTACCATTACCGG
CCTGCTGTATCTGGGCTTCTCCTTCCTGCCGGCGATCATCGGTATGGCGGTATGGACCATGAACCCGCAGCTGGAAAACA
GCGGCTTCGCCTTCTTGTTTGCCACCCAGGTGCTGCCGCCGGTGCTGGCGATGGCGATCCTGATTGCCGGGATGTCGGCC
AATATGTCTTCCGGTAGCTCTGATGCCATCGCCGCGGTGTCCATCATGCTGCGCGACCTCTACACCCTGGTGACCGGCCA
TATGCCCGCGCCGGAGAAAGCGATTCGCCTGTCGCGGATCTTCCTGGTTCTGGTGATTGCGCTGGCGCTGCTGTTTGCCC
TGACCTCCAACGACATCATCAGCTACATCACCAAAATGATCTCAATGATTATGTCCGGGATGTTTATCTGCACCATGCTT
GGCCGTTTCTGGACACGCTTTAACTGGCAGGGCGCGGTCGCCGCGCTGGCCGGCGGCGCCGGGGCATCGGTTGCGGTGCT
GGTGGATAGCGACTGGCTGGCGTTCTGGGGCAACCCGTGTATTCCGGCGGTGCTGACGAGCCTGGTGGCTTCGGTTGTGG
TGACGTTACTGACCCCGGCCAGCAGCATGAGCCGCGAGCAGGCTCTGGAGATGATTACCCGCGAGCGTGAAAGCCGCCCA
ACGCCGCCGCCGCTGCCGCTGCCGATTGGCAAACGTCATACCTCACGCGGGTAA

Upstream 100 bases:

>100_bases
CGGTGCCACTGGGTTCGCTATGCCTTTTTTCAGGCATGGTTCCTGTTAACTCATAATATTCAGCGGCACGAATATTATTT
ATTACGTCTGGATGTGAGCC

Downstream 100 bases:

>100_bases
GGGGTAATCATGAACGAGTATATTGCCGTCGATTGGGGCTCCACCCAGCTGCGGGCATGGCGGATGCGTGATGGCGAATG
CATCGACAAGCTGAAACTGC

Product: sodium/panthothenate symporter

Products: Na (I) [Cytoplasm]; pantothenate [Cytoplasm] [C]

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MNNMSFMIWFSVYACAMITLGWYVSRKQKTGEDFLLGGRSLPMILTLGSTVGTMVGTGSSVGAVGFGYSNGWAGMLYGLG
GAVGILLVAWLFAPVRKLRFMTMSEEMSYYTGGSKIIKNLVAILIFIASIGWLGAHILGGGLYLAWASGIDINVAKIIIA
LAFVVYVGIGGYSAVVWIDTIQSIVLFVGFILMAILAVHHVGGWSHIQQAVDPAAQSLFAVDKLGVLPALSLAMVIGVGV
LATPSYRQRIYSAKTVSSVRQSFTITGLLYLGFSFLPAIIGMAVWTMNPQLENSGFAFLFATQVLPPVLAMAILIAGMSA
NMSSGSSDAIAAVSIMLRDLYTLVTGHMPAPEKAIRLSRIFLVLVIALALLFALTSNDIISYITKMISMIMSGMFICTML
GRFWTRFNWQGAVAALAGGAGASVAVLVDSDWLAFWGNPCIPAVLTSLVASVVVTLLTPASSMSREQALEMITRERESRP
TPPPLPLPIGKRHTSRG

Sequences:

>Translated_497_residues
MNNMSFMIWFSVYACAMITLGWYVSRKQKTGEDFLLGGRSLPMILTLGSTVGTMVGTGSSVGAVGFGYSNGWAGMLYGLG
GAVGILLVAWLFAPVRKLRFMTMSEEMSYYTGGSKIIKNLVAILIFIASIGWLGAHILGGGLYLAWASGIDINVAKIIIA
LAFVVYVGIGGYSAVVWIDTIQSIVLFVGFILMAILAVHHVGGWSHIQQAVDPAAQSLFAVDKLGVLPALSLAMVIGVGV
LATPSYRQRIYSAKTVSSVRQSFTITGLLYLGFSFLPAIIGMAVWTMNPQLENSGFAFLFATQVLPPVLAMAILIAGMSA
NMSSGSSDAIAAVSIMLRDLYTLVTGHMPAPEKAIRLSRIFLVLVIALALLFALTSNDIISYITKMISMIMSGMFICTML
GRFWTRFNWQGAVAALAGGAGASVAVLVDSDWLAFWGNPCIPAVLTSLVASVVVTLLTPASSMSREQALEMITRERESRP
TPPPLPLPIGKRHTSRG
>Mature_497_residues
MNNMSFMIWFSVYACAMITLGWYVSRKQKTGEDFLLGGRSLPMILTLGSTVGTMVGTGSSVGAVGFGYSNGWAGMLYGLG
GAVGILLVAWLFAPVRKLRFMTMSEEMSYYTGGSKIIKNLVAILIFIASIGWLGAHILGGGLYLAWASGIDINVAKIIIA
LAFVVYVGIGGYSAVVWIDTIQSIVLFVGFILMAILAVHHVGGWSHIQQAVDPAAQSLFAVDKLGVLPALSLAMVIGVGV
LATPSYRQRIYSAKTVSSVRQSFTITGLLYLGFSFLPAIIGMAVWTMNPQLENSGFAFLFATQVLPPVLAMAILIAGMSA
NMSSGSSDAIAAVSIMLRDLYTLVTGHMPAPEKAIRLSRIFLVLVIALALLFALTSNDIISYITKMISMIMSGMFICTML
GRFWTRFNWQGAVAALAGGAGASVAVLVDSDWLAFWGNPCIPAVLTSLVASVVVTLLTPASSMSREQALEMITRERESRP
TPPPLPLPIGKRHTSRG

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=497, Percent_Identity=23.943661971831, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI14140236, Length=434, Percent_Identity=24.6543778801843, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4507031, Length=432, Percent_Identity=23.3796296296296, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI110835708, Length=462, Percent_Identity=20.3463203463203, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI87082237, Length=447, Percent_Identity=25.7270693512304, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787251, Length=501, Percent_Identity=23.1536926147705, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790503, Length=441, Percent_Identity=21.9954648526077, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17539284, Length=442, Percent_Identity=23.5294117647059, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53037; Mature: 53037

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNMSFMIWFSVYACAMITLGWYVSRKQKTGEDFLLGGRSLPMILTLGSTVGTMVGTGSS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEECCCCCCEEEEECCHHHHHHCCCCC
VGAVGFGYSNGWAGMLYGLGGAVGILLVAWLFAPVRKLRFMTMSEEMSYYTGGSKIIKNL
CCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VAILIFIASIGWLGAHILGGGLYLAWASGIDINVAKIIIALAFVVYVGIGGYSAVVWIDT
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IQSIVLFVGFILMAILAVHHVGGWSHIQQAVDPAAQSLFAVDKLGVLPALSLAMVIGVGV
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LATPSYRQRIYSAKTVSSVRQSFTITGLLYLGFSFLPAIIGMAVWTMNPQLENSGFAFLF
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEH
ATQVLPPVLAMAILIAGMSANMSSGSSDAIAAVSIMLRDLYTLVTGHMPAPEKAIRLSRI
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FLVLVIALALLFALTSNDIISYITKMISMIMSGMFICTMLGRFWTRFNWQGAVAALAGGA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCC
GASVAVLVDSDWLAFWGNPCIPAVLTSLVASVVVTLLTPASSMSREQALEMITRERESRP
CCEEEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
TPPPLPLPIGKRHTSRG
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNNMSFMIWFSVYACAMITLGWYVSRKQKTGEDFLLGGRSLPMILTLGSTVGTMVGTGSS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEECCCCCCEEEEECCHHHHHHCCCCC
VGAVGFGYSNGWAGMLYGLGGAVGILLVAWLFAPVRKLRFMTMSEEMSYYTGGSKIIKNL
CCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VAILIFIASIGWLGAHILGGGLYLAWASGIDINVAKIIIALAFVVYVGIGGYSAVVWIDT
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IQSIVLFVGFILMAILAVHHVGGWSHIQQAVDPAAQSLFAVDKLGVLPALSLAMVIGVGV
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LATPSYRQRIYSAKTVSSVRQSFTITGLLYLGFSFLPAIIGMAVWTMNPQLENSGFAFLF
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEH
ATQVLPPVLAMAILIAGMSANMSSGSSDAIAAVSIMLRDLYTLVTGHMPAPEKAIRLSRI
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FLVLVIALALLFALTSNDIISYITKMISMIMSGMFICTMLGRFWTRFNWQGAVAALAGGA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCC
GASVAVLVDSDWLAFWGNPCIPAVLTSLVASVVVTLLTPASSMSREQALEMITRERESRP
CCEEEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
TPPPLPLPIGKRHTSRG
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; pantothenate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + pantothenate [Periplasm] = Na (I) [Cytoplasm] + pantothenate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]