Click here to switch to the map view.

The map label for this gene is dnaB [H]

Identifier: 238892525

GI number: 238892525

Start: 306896

End: 308311

Strand: Direct

Name: dnaB [H]

Synonym: KP1_0294

Alternate gene names: 238892525

Gene position: 306896-308311 (Clockwise)

Preceding gene: 238892523

Following gene: 238892526

Centisome position: 5.85

GC content: 59.53

Gene sequence:

>1416_bases
ATGGCAGGAAATAAACCCTTCAACAAACCACAGACAGAAACCCGCGAACGCGATCCGCAGCTCGCCGGGCTGAAAGTCCC
GCCGCACTCGATTGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTGGATAACGAGCGCTGGGACGACGTCGCCGAAC
GCGTCGTTGCCGACGACTTTTATACCCGTCCGCACCGTCATATTTTTACCGAGATGGCGCGCCTGCAGGAGTCCGGCAGC
CCGATTGACCTGATTACGCTCGCCGAGTCGCTGGAGCGTCAGGGGCAGCTGGACAGCGTGGGCGGCTTCGCCTATCTGGC
CGAACTGTCCAAAAACACGCCCAGCGCGGCTAACATCAGCGCCTATGCCGACATCGTGCGCGAACGCGCGGTGGTGCGCG
AAATGATTTCGGTGGCCAACGAGATCGCCGAAGCCGGCTTCGACCCACAGGGGCGAACCAGCGAAGATCTGCTCGACCTC
GCCGAATCCCGGGTTTTCAAAATCGCCGAAAGCCGGGCCAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTCCTCGA
TGCCACCGTCGCCCGTATCGAACAGCTGTTCCAGCAGCCCCATGACGGCGTCACCGGGGTCAATACCGGCTACGACGACC
TCAACAAAAAGACCGCCGGCCTGCAGCCGTCGGATCTGATTATCGTCGCCGCCCGTCCATCGATGGGTAAAACGACCTTC
GCGATGAACCTCGTGGAAAACGCGGCGATGCTGCAGGACAAACCGGTATTAATCTTCAGTCTCGAGATGCCCTCGGAACA
GATCATGATGCGTTCTCTGGCCTCGCTGTCGCGCGTCGATCAGACCCGCATTCGTACCGGTCAACTGGACGACGAAGACT
GGGCGCGGATCTCCGGCACCATGGGCATCCTGCTGGAAAAGCGCAATATCTATATCGATGACTCCTCCGGCCTGACGCCG
ACGGAGGTGCGCTCCCGCGCGCGGCGTATCGCCCGCGAGCACGGCGGCATCGGACTGATTATGATCGACTACCTGCAGCT
GATGCGCGTGCCGTCGCTCTCCGACAACCGTACCCTTGAGATTGCCGAAATCTCCCGCTCGCTGAAGGCGCTGGCGAAAG
AGCTGCAGGTGCCGGTGGTGGCGCTGTCGCAGCTTAACCGCTCGCTGGAGCAGCGCGCCGATAAGCGCCCGGTCAACTCC
GACCTGCGTGAGTCCGGCTCTATCGAACAGGATGCCGACTTAATCATGTTCATTTACCGTGATGAGGTCTATCACGAGAA
CAGCGATCTTAAAGGCATCGCGGAAATTATCATTGGTAAACAACGTAACGGCCCGATCGGCACCGTGCGACTGACCTTTA
ACGGCCAGTGGTCGCGTTTCGATAATTATGCCGGTCCTCAGTATGATGATGAGTAA

Upstream 100 bases:

>100_bases
TTACCCGCCTCAGGCCTGCCACGCTTAATGGGTTCTTTAGCGGCAATGGCTTTCTCGCTATACTGTCACGCTCTTTTCCG
GATCGGTGAAATGTTAGGCT

Downstream 100 bases:

>100_bases
TCCTTCCGCCTTCTTTTATTAAGGAATTCAAATGCAAGCGGCAACTGTAGTTATCAACCGCCGCGCTCTGCGACACAACC
TGCAACGTCTGCGTGAACTG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MAGNKPFNKPQTETRERDPQLAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMARLQESGS
PIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTF
AMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTRIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTP
TEVRSRARRIAREHGGIGLIMIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNS
DLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE

Sequences:

>Translated_471_residues
MAGNKPFNKPQTETRERDPQLAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMARLQESGS
PIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTF
AMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTRIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTP
TEVRSRARRIAREHGGIGLIMIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNS
DLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE
>Mature_470_residues
AGNKPFNKPQTETRERDPQLAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSP
IDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLA
ESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTFA
MNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTRIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPT
EVRSRARRIAREHGGIGLIMIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSD
LRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=471, Percent_Identity=98.5138004246285, Blast_Score=945, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52466; Mature: 52335

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGNKPFNKPQTETRERDPQLAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDF
CCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHCCHHCCCCHHHHHHHHHHHHHH
YTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANIS
HCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHH
AYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLAESRVFKIAESRANKDEGPK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
NIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHCCCCCCCEEEEEECCCCCCHHH
AMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTRIRTGQLDDEDWARISGT
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
MGILLEKRNIYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLMRVPSLSDNRTLE
EEEEEEECEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEHHHHHHHHHCCCCCCCCCEEH
IAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEE
DEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE
HHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AGNKPFNKPQTETRERDPQLAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDF
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHCCHHCCCCHHHHHHHHHHHHHH
YTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANIS
HCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHH
AYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLAESRVFKIAESRANKDEGPK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
NIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHCCCCCCCEEEEEECCCCCCHHH
AMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTRIRTGQLDDEDWARISGT
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
MGILLEKRNIYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLMRVPSLSDNRTLE
EEEEEEECEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEHHHHHHHHHCCCCCCCCCEEH
IAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEE
DEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE
HHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]