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The map label for this gene is tnpR [H]

Identifier: 238028390

GI number: 238028390

Start: 3234763

End: 3235338

Strand: Direct

Name: tnpR [H]

Synonym: bglu_1g28510

Alternate gene names: 238028390

Gene position: 3234763-3235338 (Clockwise)

Preceding gene: 238028389

Following gene: 238028391

Centisome position: 82.8

GC content: 64.58

Gene sequence:

>576_bases
ATGTCCCGAACCTTTGCCTATGCCCGTGTCAGTACGTCCGACCAGACCACCGCGAACCAGTTACGAGAGATTGAGGCGGC
CGGCTTCTCGGTCGACAAGCGCCGTGTTGTGTCGGAAAGCATTTCGGGGAGCGTAAGCGCCGATCAGCGGCCGGGGTTTG
CGCAGTTGCTCGTCAAGATGGAAGAAGGCGACGTGTTGATCGTGACGAAGCTCGACCGACTCGGCCGCAATGCGATGGAC
GTGCGGGCCACGGTCGAGGGATTGGCCGAACGCGGTATCCGGGTTCATTGTCTCGCCCTGGGCGGCGTCGATCTGACTAG
CGCGGCCGGCCGGATGACGATGCAGGTATTGAACGCGGTGGCCGAATTCGAGCGGGATCTGTTGATTGAGCGCACGCACG
CTGGTATCGCTCGCGCGAAGGCGGAAGGCAAGGCGATGGGGCGGCCGTCCGCCCTGTCAAACGAGCAGCGGGCGGACGTG
CTGCGCGAGTTGGACGCGGGGGCAAGCGTGGCCGCGCTTGCTCGGCGATTTGGCACGAGCCGCCAGACGATCATGCGGGT
ACGCGACGCAGCCTAA

Upstream 100 bases:

>100_bases
GCGGACAGGGGCATTTTGCCCTGTCCGTAAATAAGGGTTGAAATGTTTTGGACATCGCCTTACATTCGGACGTCCATTTC
GTACAGATTGAGTGCCCGAT

Downstream 100 bases:

>100_bases
GAGGCCAGTTCAAAAACTCCCGAGAGGGGCTGTTTACTTCCTCGGCAAGCTGTTAACCTTGGGCATCAGAACAACGGAAG
TCCAAGGATGGAAGCGCCAA

Product: resolvase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MSRTFAYARVSTSDQTTANQLREIEAAGFSVDKRRVVSESISGSVSADQRPGFAQLLVKMEEGDVLIVTKLDRLGRNAMD
VRATVEGLAERGIRVHCLALGGVDLTSAAGRMTMQVLNAVAEFERDLLIERTHAGIARAKAEGKAMGRPSALSNEQRADV
LRELDAGASVAALARRFGTSRQTIMRVRDAA

Sequences:

>Translated_191_residues
MSRTFAYARVSTSDQTTANQLREIEAAGFSVDKRRVVSESISGSVSADQRPGFAQLLVKMEEGDVLIVTKLDRLGRNAMD
VRATVEGLAERGIRVHCLALGGVDLTSAAGRMTMQVLNAVAEFERDLLIERTHAGIARAKAEGKAMGRPSALSNEQRADV
LRELDAGASVAALARRFGTSRQTIMRVRDAA
>Mature_190_residues
SRTFAYARVSTSDQTTANQLREIEAAGFSVDKRRVVSESISGSVSADQRPGFAQLLVKMEEGDVLIVTKLDRLGRNAMDV
RATVEGLAERGIRVHCLALGGVDLTSAAGRMTMQVLNAVAEFERDLLIERTHAGIARAKAEGKAMGRPSALSNEQRADVL
RELDAGASVAALARRFGTSRQTIMRVRDAA

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787638, Length=188, Percent_Identity=61.1702127659574, Blast_Score=247, Evalue=3e-67,
Organism=Escherichia coli, GI1787827, Length=188, Percent_Identity=60.6382978723404, Blast_Score=246, Evalue=8e-67,
Organism=Escherichia coli, GI1787404, Length=187, Percent_Identity=38.5026737967914, Blast_Score=109, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 20588; Mature: 20457

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTFAYARVSTSDQTTANQLREIEAAGFSVDKRRVVSESISGSVSADQRPGFAQLLVKM
CCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHEEE
EEGDVLIVTKLDRLGRNAMDVRATVEGLAERGIRVHCLALGGVDLTSAAGRMTMQVLNAV
CCCCEEEEECHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
AEFERDLLIERTHAGIARAKAEGKAMGRPSALSNEQRADVLRELDAGASVAALARRFGTS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCC
RQTIMRVRDAA
HHHHHHHHCCC
>Mature Secondary Structure 
SRTFAYARVSTSDQTTANQLREIEAAGFSVDKRRVVSESISGSVSADQRPGFAQLLVKM
CCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHEEE
EEGDVLIVTKLDRLGRNAMDVRATVEGLAERGIRVHCLALGGVDLTSAAGRMTMQVLNAV
CCCCEEEEECHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
AEFERDLLIERTHAGIARAKAEGKAMGRPSALSNEQRADVLRELDAGASVAALARRFGTS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCC
RQTIMRVRDAA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3027041 [H]