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The map label for this gene is metG [H]

Identifier: 238028342

GI number: 238028342

Start: 3178789

End: 3180945

Strand: Direct

Name: metG [H]

Synonym: bglu_1g28030

Alternate gene names: 238028342

Gene position: 3178789-3180945 (Clockwise)

Preceding gene: 238028341

Following gene: 238028345

Centisome position: 81.37

GC content: 66.67

Gene sequence:

>2157_bases
ATGTCCGCATCCGATCTCAATACCGTGCAGGCCGGCGCACCCTCGAGCCGCCGCCAGATCCTCGTCACTTCCGCACTGCC
CTACGCGAACGGGCAAATCCACATCGGCCACCTGGTCGAGTACATCCAGACCGACATCTGGGTGCGGACGCTGCGAATGC
ACGGCCACGAGGTCTATTACATCGGCGCCGACGACACGCACGGCACGCCGATCATGCTGCGCGCCGAGCAGGAAGGCATC
ACGCCCAAGCAGCTGATCGAGCGCGTCTGGCGCGAGCACAAGCGTGACTTCGACAGCTACGCGATCTCGTTCGACAACTT
CTACACCACGGACTCCGACGAGAACCGCGTGCTGAGCGAGACCATCTACCTGGCGCTGAAGGATGCCGGGCTGATCGCCG
AGCGCGACATCGAGCAGGCTTACGACCCCGTCAAGCAAATGTTCCTGCCGGACCGCTTCATCAAGGGCGAGTGCCCGAAG
TGCCACGCGAAGGACCAGTACGGCGACAGCTGCGAGGTGTGCGGCTCGACCTATCAGCCCACCGAGCTGCTCAATCCGTA
CTCGGTGGTGTCGGGCGCCACGCCGGTGCGCAAGACGTCCACGCATCACTTCTTCAAGCTGTCCGACCCGCGCTGCGAAA
ACTTCCTGCGCGGCTGGGTGGGCGGGCTCGCGCAGCCCGAGGCCACCAACAAGATGCGCGAATGGCTCGGCGAGGCCGGC
GAAGCGAAGCTGGCCGACTGGGACATCTCGCGCGATGCGCCCTACTTCGGCTTCGAGATCCCCGGCGCGCCCGGCAAGTA
CTTCTATGTCTGGCTCGACGCGCCGGTGGGCTACTACGCGAGCTTCAAGAACCACTGCGAGAAGCTCGGGCTCGACTTCG
ACGCCTGGACGCGTCCCGGCTCGACGACCGAGCAGTATCACTTCATCGGCAAGGACATCCTTTATTTCCACACGCTGTTC
TGGCCCGCGATGCTCGAGTTCTCGGGCCACCGCACGCCGACCAACGTGTTCGCGCACGGCTTCCTGACCGTGGACGGCGC
CAAGATGTCGAAGTCGCGCGGCACCTTCATCACGGCGCAGAGCTATATCGACGCGGGCCTGAACCCGGAGTGGCTGCGCT
ACTACTTCGCCGCGAAGCTGAACGCGACGATGGAAGACCTCGACCTGAATCTCGAGGACTTCCAGGCGCGCGTGAACAGC
GACCTGGTGGGCAAGTACGTGAACATCGCGAGCCGGGCGGCGGGCTTCCTGATCAAGCGCTTCGAGGGCCGCGTGCAGGA
CAGCGCGATGAACCACCCGCTGCTCGCCTCGCTGCGCGGCACGATTCCGCAGATCGCCGCGCACTACGAGGCGCGCGAAT
ACGGCCGCGCGCTGCGCCAGACCATGGAGCTGGCCGACGCCGTGAACGGCTACGTCGACACCGCCAAGCCCTGGGAGCAG
GCCAAGGACCCGGCCAACGCGGTGGCGCTGCACGAGACCTGCAGCGTGAGCCTGGAGGCGTTCCGCCTGCTGTCGCTCGC
GCTCAAGCCGGTGCTGCCGCGCGTGGTCGAAGCGGTCGAGGCGTTCCTCGGCATCGCGCCGCTGACCTGGGCGAGCGTCG
CGACGCCGCTCTCGTCGACGCAGCCCGTCAACGCCTACCAGCACCTGATGACGCGCGTCGATCCGAAGCAGATCGCGGCG
CTGCTGGAGGCGAACCACGGCTCGCTCGCGGCGGCCGACGCGGCGGCCAGTGCCGCGAGCGCCGATCCGAAGGCGAAAGG
CGCCAAGGACGCCAAGCCGGCCAAGGACGCGAAGACCGCGGCCGCGACCGGCGACGGCACGATCTCGATCGACGACTTCA
CGAAGATCGACCTGCGGATCGCGAAGATCGTCGCGTGCCAGGCCGTGGAGGGCTCGGACAAGCTGCTGCAGCTCACGCTC
GACGTGGGCGAGGAAAAGACGCGCAACGTGTTCTCGGGGATCAAGTCGGCCTACCGGCCCGAGGATCTGGTGGGCAAGCT
GACCGTGATGGTCGCCAACCTCGCGCCGCGCAAGATGAAGTTCGGCATGTCCGAAGGCATGGTGCTGGCCGCCTCGGCCA
AGGACGAGAAGTCGGAACCGGGCCTCTACATCCTCGAGCCGCACAGCGGCGCCAAGCCGGGCATGCGCGTGAGCTGA

Upstream 100 bases:

>100_bases
CCGCCACGCGGGGAGCACTCGCGCACCGCCTGCTAGAATCTCGCATTCCGACGCATTCCCGACCCGAGCCGCGACCTGCG
CGGCCATCCCTGACGACCCT

Downstream 100 bases:

>100_bases
AGGCGCGCCCCCGCCGCGCTTCATGCGCTTGCCGCGGCACCCGGAAAAAAACCCGCTCGACGAGCGGGTTTTTTCATGCG
CGCGGCGGCAACGCCGTCAG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MSASDLNTVQAGAPSSRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYIGADDTHGTPIMLRAEQEGI
TPKQLIERVWREHKRDFDSYAISFDNFYTTDSDENRVLSETIYLALKDAGLIAERDIEQAYDPVKQMFLPDRFIKGECPK
CHAKDQYGDSCEVCGSTYQPTELLNPYSVVSGATPVRKTSTHHFFKLSDPRCENFLRGWVGGLAQPEATNKMREWLGEAG
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNHCEKLGLDFDAWTRPGSTTEQYHFIGKDILYFHTLF
WPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDAGLNPEWLRYYFAAKLNATMEDLDLNLEDFQARVNS
DLVGKYVNIASRAAGFLIKRFEGRVQDSAMNHPLLASLRGTIPQIAAHYEAREYGRALRQTMELADAVNGYVDTAKPWEQ
AKDPANAVALHETCSVSLEAFRLLSLALKPVLPRVVEAVEAFLGIAPLTWASVATPLSSTQPVNAYQHLMTRVDPKQIAA
LLEANHGSLAAADAAASAASADPKAKGAKDAKPAKDAKTAAATGDGTISIDDFTKIDLRIAKIVACQAVEGSDKLLQLTL
DVGEEKTRNVFSGIKSAYRPEDLVGKLTVMVANLAPRKMKFGMSEGMVLAASAKDEKSEPGLYILEPHSGAKPGMRVS

Sequences:

>Translated_718_residues
MSASDLNTVQAGAPSSRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYIGADDTHGTPIMLRAEQEGI
TPKQLIERVWREHKRDFDSYAISFDNFYTTDSDENRVLSETIYLALKDAGLIAERDIEQAYDPVKQMFLPDRFIKGECPK
CHAKDQYGDSCEVCGSTYQPTELLNPYSVVSGATPVRKTSTHHFFKLSDPRCENFLRGWVGGLAQPEATNKMREWLGEAG
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNHCEKLGLDFDAWTRPGSTTEQYHFIGKDILYFHTLF
WPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDAGLNPEWLRYYFAAKLNATMEDLDLNLEDFQARVNS
DLVGKYVNIASRAAGFLIKRFEGRVQDSAMNHPLLASLRGTIPQIAAHYEAREYGRALRQTMELADAVNGYVDTAKPWEQ
AKDPANAVALHETCSVSLEAFRLLSLALKPVLPRVVEAVEAFLGIAPLTWASVATPLSSTQPVNAYQHLMTRVDPKQIAA
LLEANHGSLAAADAAASAASADPKAKGAKDAKPAKDAKTAAATGDGTISIDDFTKIDLRIAKIVACQAVEGSDKLLQLTL
DVGEEKTRNVFSGIKSAYRPEDLVGKLTVMVANLAPRKMKFGMSEGMVLAASAKDEKSEPGLYILEPHSGAKPGMRVS
>Mature_717_residues
SASDLNTVQAGAPSSRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYIGADDTHGTPIMLRAEQEGIT
PKQLIERVWREHKRDFDSYAISFDNFYTTDSDENRVLSETIYLALKDAGLIAERDIEQAYDPVKQMFLPDRFIKGECPKC
HAKDQYGDSCEVCGSTYQPTELLNPYSVVSGATPVRKTSTHHFFKLSDPRCENFLRGWVGGLAQPEATNKMREWLGEAGE
AKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNHCEKLGLDFDAWTRPGSTTEQYHFIGKDILYFHTLFW
PAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDAGLNPEWLRYYFAAKLNATMEDLDLNLEDFQARVNSD
LVGKYVNIASRAAGFLIKRFEGRVQDSAMNHPLLASLRGTIPQIAAHYEAREYGRALRQTMELADAVNGYVDTAKPWEQA
KDPANAVALHETCSVSLEAFRLLSLALKPVLPRVVEAVEAFLGIAPLTWASVATPLSSTQPVNAYQHLMTRVDPKQIAAL
LEANHGSLAAADAAASAASADPKAKGAKDAKPAKDAKTAAATGDGTISIDDFTKIDLRIAKIVACQAVEGSDKLLQLTLD
VGEEKTRNVFSGIKSAYRPEDLVGKLTVMVANLAPRKMKFGMSEGMVLAASAKDEKSEPGLYILEPHSGAKPGMRVS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=590, Percent_Identity=26.1016949152542, Blast_Score=209, Evalue=8e-54,
Organism=Homo sapiens, GI24308436, Length=553, Percent_Identity=25.8589511754069, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI4507947, Length=149, Percent_Identity=31.5436241610738, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788432, Length=707, Percent_Identity=53.041018387553, Blast_Score=736, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=602, Percent_Identity=27.2425249169435, Blast_Score=225, Evalue=8e-59,
Organism=Caenorhabditis elegans, GI212645096, Length=546, Percent_Identity=22.8937728937729, Blast_Score=123, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71992781, Length=283, Percent_Identity=25.7950530035336, Blast_Score=96, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6321704, Length=565, Percent_Identity=27.0796460176991, Blast_Score=219, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321610, Length=545, Percent_Identity=24.954128440367, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24655311, Length=635, Percent_Identity=28.0314960629921, Blast_Score=212, Evalue=8e-55,
Organism=Drosophila melanogaster, GI21358483, Length=552, Percent_Identity=26.4492753623188, Blast_Score=132, Evalue=9e-31,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 79228; Mature: 79097

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASDLNTVQAGAPSSRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYY
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEE
IGADDTHGTPIMLRAEQEGITPKQLIERVWREHKRDFDSYAISFDNFYTTDSDENRVLSE
EECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEEECCEECCCCCCCHHHHH
TIYLALKDAGLIAERDIEQAYDPVKQMFLPDRFIKGECPKCHAKDQYGDSCEVCGSTYQP
HHEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHHCCCCCCC
TELLNPYSVVSGATPVRKTSTHHFFKLSDPRCENFLRGWVGGLAQPEATNKMREWLGEAG
HHHCCCHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNHCEKLGLDFDAWTRPG
CCEEECCCCCCCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHCCCCC
STTEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCCCCCCCCCEEEEH
SYIDAGLNPEWLRYYFAAKLNATMEDLDLNLEDFQARVNSDLVGKYVNIASRAAGFLIKR
HHHCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FEGRVQDSAMNHPLLASLRGTIPQIAAHYEAREYGRALRQTMELADAVNGYVDTAKPWEQ
HHCHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
AKDPANAVALHETCSVSLEAFRLLSLALKPVLPRVVEAVEAFLGIAPLTWASVATPLSST
CCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
QPVNAYQHLMTRVDPKQIAALLEANHGSLAAADAAASAASADPKAKGAKDAKPAKDAKTA
CCCHHHHHHHHCCCHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
AATGDGTISIDDFTKIDLRIAKIVACQAVEGSDKLLQLTLDVGEEKTRNVFSGIKSAYRP
CCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
EDLVGKLTVMVANLAPRKMKFGMSEGMVLAASAKDEKSEPGLYILEPHSGAKPGMRVS
HHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SASDLNTVQAGAPSSRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYY
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEE
IGADDTHGTPIMLRAEQEGITPKQLIERVWREHKRDFDSYAISFDNFYTTDSDENRVLSE
EECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEEECCEECCCCCCCHHHHH
TIYLALKDAGLIAERDIEQAYDPVKQMFLPDRFIKGECPKCHAKDQYGDSCEVCGSTYQP
HHEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHHCCCCCCC
TELLNPYSVVSGATPVRKTSTHHFFKLSDPRCENFLRGWVGGLAQPEATNKMREWLGEAG
HHHCCCHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNHCEKLGLDFDAWTRPG
CCEEECCCCCCCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHCCCCC
STTEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCCCCCCCCCEEEEH
SYIDAGLNPEWLRYYFAAKLNATMEDLDLNLEDFQARVNSDLVGKYVNIASRAAGFLIKR
HHHCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FEGRVQDSAMNHPLLASLRGTIPQIAAHYEAREYGRALRQTMELADAVNGYVDTAKPWEQ
HHCHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
AKDPANAVALHETCSVSLEAFRLLSLALKPVLPRVVEAVEAFLGIAPLTWASVATPLSST
CCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
QPVNAYQHLMTRVDPKQIAALLEANHGSLAAADAAASAASADPKAKGAKDAKPAKDAKTA
CCCHHHHHHHHCCCHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
AATGDGTISIDDFTKIDLRIAKIVACQAVEGSDKLLQLTLDVGEEKTRNVFSGIKSAYRP
CCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
EDLVGKLTVMVANLAPRKMKFGMSEGMVLAASAKDEKSEPGLYILEPHSGAKPGMRVS
HHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA