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The map label for this gene is leuA [H]

Identifier: 238028152

GI number: 238028152

Start: 2966446

End: 2967990

Strand: Reverse

Name: leuA [H]

Synonym: bglu_1g26030

Alternate gene names: 238028152

Gene position: 2967990-2966446 (Counterclockwise)

Preceding gene: 238028153

Following gene: 238028148

Centisome position: 75.98

GC content: 66.15

Gene sequence:

>1545_bases
ATGACCGACAAGCTGATTATTTTCGATACGACCCTGCGTGACGGCGAGCAGTCGCCCGGCGCGTCGATGACGAAGGAAGA
GAAGATCCGTATCGCCAGACAGCTCGAGCGGATGAAGGTCGACGTGATCGAGGCCGGCTTCGCGGCCAGCTCGAACGGCG
ATTTCGACGCGATCCACACCATTGCGGGGCTCGTGAAGGACAGCACGATCTGTTCGCTCGCGCGCGCCAACGACAAGGAC
ATCCAGCGCGCGGCGGACGCGCTGAAGCCGGCGGAACGCTTCCGGATCCATACCTTCATCGCCACCTCGCCGCTGCACAT
GGAGAAGAAGCTGCGCATGACGCCGGACCAGGTGTTCGAGCAGGCGCGCCTTGCCGTGCGCTTCGCGCGCAAGTTCACCG
ACGACGTCGAGTTCTCGCCCGAGGACGGCAGCCGCTCCGACATGGATTTCCTCTGCCGCGTGCTGGAGGCCGTGATCGCC
GAGGGCGCGACCACCATCAATATCGCCGACACGGTCGGCTATGGCGTGCCCGAGCTCTACGGCAACCTCGTGAAGACGCT
GCGCGAGCGCATTCCGAATTCGGACAAGGCGATCTTCTCGGTTCACTGCCACAACGACCTGGGCATGGCGGTGGCGAACT
CGCTGGCCGGCGTGCAGATCGGCGGCGCGCGTCAGGTCGAATGCACCGTCAACGGCCTCGGCGAGCGCGCCGGCAACACC
TCGCTCGAGGAGATCGTGATGGCGGTGAAGACGCGCCGCGACTACTTCGGGCTCGACGTCGGCCTCGACACCACGCAGAT
CGTGCCGGCCTCGAAGCTGGTCTCGCAGATCACCGGCTTCGTCGTGCAGCCCAACAAGGCGGTGGTCGGCGCGAACGCGT
TCGCGCACGCCTCGGGCATCCACCAGGACGGCGTGCTGAAGGCGCGCGACACCTACGAGATCATGCGCGCCGAAGACGTG
GGCTGGACCGCCAACAAGATCGTGCTCGGCAAGCTGTCCGGCCGCAACGCGTTCAAGCAGCGCCTGCAGGAGCTCGGCGT
ATCGCTCGACAGCGAGGGCGAACTGAACGCCGCGTTCATGCGCTTCAAGGATCTTGCGGACCGCAAGGCCGAGATCTTCG
ACGAGGACATCATCGCGATCGTCTCGGAGGAAGCGGCGCTCGCGCACGAACCCGAGCACTACAAGTTCGTGTCGCTGGCG
CAGCGCTCGGAGACGGGCGAGCAGCCGCATGCGAAGGTCGTGTTTGCCGCCGATGGCGGCGAGGTGACGGGCGAGGCGCG
CGGCAACGGGCCGGTGGACGCGACCTTCAACGCGATCGAGACCGAGGTGGGCAGCGGCGCCGAGCTCGTGCTGTATTCGG
TCAACGCGATCACGACCGGCACGCAGGCGCAGGGCGAGGTGACCGTGCGGCTCTCCAAGAGCGGCCGGATCGTCAACGGC
GTCGGCACCGATCCCGACATCGTCGCCGCGTCGGCGAAGGCCTATCTGTCGGCGCTGAGCAAGCTGCATTCGAACATCGA
CAAGCTGAACCCGCAGCTGTCCTGA

Upstream 100 bases:

>100_bases
ACCGTCATCGCCCCCGTCGAGCTTCGTCGATTGTCGAGCGTCGCCAGCGGTGGCGCGACCTACATCGTTTGGTTTCACCC
ATAAGATTCCCGGAGCCCCC

Downstream 100 bases:

>100_bases
TGCCGGGCCGCCGGCCCGGCGGCGCAACGATACGACGCCCCACGCTGCCGCCGGCCGCGTGGGGCGTCGTCGTTTGGGGG
CGCGTTATTGCGGCGCCGGG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTDKLIIFDTTLRDGEQSPGASMTKEEKIRIARQLERMKVDVIEAGFAASSNGDFDAIHTIAGLVKDSTICSLARANDKD
IQRAADALKPAERFRIHTFIATSPLHMEKKLRMTPDQVFEQARLAVRFARKFTDDVEFSPEDGSRSDMDFLCRVLEAVIA
EGATTINIADTVGYGVPELYGNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVQIGGARQVECTVNGLGERAGNT
SLEEIVMAVKTRRDYFGLDVGLDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQELGVSLDSEGELNAAFMRFKDLADRKAEIFDEDIIAIVSEEAALAHEPEHYKFVSLA
QRSETGEQPHAKVVFAADGGEVTGEARGNGPVDATFNAIETEVGSGAELVLYSVNAITTGTQAQGEVTVRLSKSGRIVNG
VGTDPDIVAASAKAYLSALSKLHSNIDKLNPQLS

Sequences:

>Translated_514_residues
MTDKLIIFDTTLRDGEQSPGASMTKEEKIRIARQLERMKVDVIEAGFAASSNGDFDAIHTIAGLVKDSTICSLARANDKD
IQRAADALKPAERFRIHTFIATSPLHMEKKLRMTPDQVFEQARLAVRFARKFTDDVEFSPEDGSRSDMDFLCRVLEAVIA
EGATTINIADTVGYGVPELYGNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVQIGGARQVECTVNGLGERAGNT
SLEEIVMAVKTRRDYFGLDVGLDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQELGVSLDSEGELNAAFMRFKDLADRKAEIFDEDIIAIVSEEAALAHEPEHYKFVSLA
QRSETGEQPHAKVVFAADGGEVTGEARGNGPVDATFNAIETEVGSGAELVLYSVNAITTGTQAQGEVTVRLSKSGRIVNG
VGTDPDIVAASAKAYLSALSKLHSNIDKLNPQLS
>Mature_513_residues
TDKLIIFDTTLRDGEQSPGASMTKEEKIRIARQLERMKVDVIEAGFAASSNGDFDAIHTIAGLVKDSTICSLARANDKDI
QRAADALKPAERFRIHTFIATSPLHMEKKLRMTPDQVFEQARLAVRFARKFTDDVEFSPEDGSRSDMDFLCRVLEAVIAE
GATTINIADTVGYGVPELYGNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVQIGGARQVECTVNGLGERAGNTS
LEEIVMAVKTRRDYFGLDVGLDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVG
WTANKIVLGKLSGRNAFKQRLQELGVSLDSEGELNAAFMRFKDLADRKAEIFDEDIIAIVSEEAALAHEPEHYKFVSLAQ
RSETGEQPHAKVVFAADGGEVTGEARGNGPVDATFNAIETEVGSGAELVLYSVNAITTGTQAQGEVTVRLSKSGRIVNGV
GTDPDIVAASAKAYLSALSKLHSNIDKLNPQLS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=505, Percent_Identity=49.5049504950495, Blast_Score=456, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6324682, Length=542, Percent_Identity=25.4612546125461, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324225, Length=555, Percent_Identity=25.5855855855856, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6320071, Length=378, Percent_Identity=29.1005291005291, Blast_Score=126, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6320019, Length=387, Percent_Identity=27.906976744186, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55463; Mature: 55332

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKLIIFDTTLRDGEQSPGASMTKEEKIRIARQLERMKVDVIEAGFAASSNGDFDAIHT
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAGLVKDSTICSLARANDKDIQRAADALKPAERFRIHTFIATSPLHMEKKLRMTPDQVFE
HHHHHHCHHHHHHHCCCHHHHHHHHHHHCHHHHEEEEEEEECCCHHHHHHCCCCHHHHHH
QARLAVRFARKFTDDVEFSPEDGSRSDMDFLCRVLEAVIAEGATTINIADTVGYGVPELY
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHH
GNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVQIGGARQVECTVNGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHCCEEECCCEEEEEEECCCCCCCCCC
SLEEIVMAVKTRRDYFGLDVGLDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGVSLDSEGELNAAFM
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHH
RFKDLADRKAEIFDEDIIAIVSEEAALAHEPEHYKFVSLAQRSETGEQPHAKVVFAADGG
HHHHHHHHHHHHHCCHHHHHEECCHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCC
EVTGEARGNGPVDATFNAIETEVGSGAELVLYSVNAITTGTQAQGEVTVRLSKSGRIVNG
EEECCCCCCCCCCCCHHHHHHCCCCCCEEEEEEEEEEECCCCCCCEEEEEEECCCCEEEC
VGTDPDIVAASAKAYLSALSKLHSNIDKLNPQLS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TDKLIIFDTTLRDGEQSPGASMTKEEKIRIARQLERMKVDVIEAGFAASSNGDFDAIHT
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAGLVKDSTICSLARANDKDIQRAADALKPAERFRIHTFIATSPLHMEKKLRMTPDQVFE
HHHHHHCHHHHHHHCCCHHHHHHHHHHHCHHHHEEEEEEEECCCHHHHHHCCCCHHHHHH
QARLAVRFARKFTDDVEFSPEDGSRSDMDFLCRVLEAVIAEGATTINIADTVGYGVPELY
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHH
GNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVQIGGARQVECTVNGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHCCEEECCCEEEEEEECCCCCCCCCC
SLEEIVMAVKTRRDYFGLDVGLDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGVSLDSEGELNAAFM
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHH
RFKDLADRKAEIFDEDIIAIVSEEAALAHEPEHYKFVSLAQRSETGEQPHAKVVFAADGG
HHHHHHHHHHHHHCCHHHHHEECCHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCC
EVTGEARGNGPVDATFNAIETEVGSGAELVLYSVNAITTGTQAQGEVTVRLSKSGRIVNG
EEECCCCCCCCCCCCHHHHHHCCCCCCEEEEEEEEEEECCCCCCCEEEEEEECCCCEEEC
VGTDPDIVAASAKAYLSALSKLHSNIDKLNPQLS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA