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The map label for this gene is rlmB [H]

Identifier: 238027543

GI number: 238027543

Start: 2140835

End: 2141587

Strand: Reverse

Name: rlmB [H]

Synonym: bglu_1g19610

Alternate gene names: 238027543

Gene position: 2141587-2140835 (Counterclockwise)

Preceding gene: 238027544

Following gene: 238027542

Centisome position: 54.82

GC content: 70.92

Gene sequence:

>753_bases
ATGTCACGTTTGAAGGTTCTCTACGGTTTTCACGCGGTCACCGCCCGCTTGCGGCACGATGCGTCGACGGTCGCCGAGGT
GTTGTACGACCAGACCCGGCGCGATCGCCGCATGACGGATTTCCTGCAGACCGCGAAGGAAGCCGGCGTGCGCCTGATCG
CCGCCGACGAAACGCGCTTGTGGGGTCTCGCGCACACCGAGCGCCATCAGGGCGTGGTGGCGCGCGTCGAGGATCTGCCG
CTCGCGCAGAACCTGGCGGAACTGCTCGACGGCATTCAGGGCCCGGCACTGCTGCTGGTGCTCGACGGCGTCACCGATCC
GCACAATCTCGGCGCCTGCTTGCGCGTGGCCGACGCGGCCGGCGCGCACGCGATCATCGCGCCGCGCGACCGCGCCGTCG
GCCTCAACGCGACGGCCGCCAAGGTCGCGAGCGGCGCGGCCGACACCGTGCCCTACATCACGGTGACGAATCTCGCGCGC
GCGCTGCGCGAGCTGAAGGACGCGGGCGTCTGGGTGATCGGCACGGCCGACGACGCGCCGGCGAGCCTCTACGAGACCAA
GCTCGACGGCCCGGTGGCGCTGGTGATGGGCGCCGAGGGCGAGGGCATGCGCCGCCTCACGCGCGATACCTGCGACGACG
TGATGAACCTGCCGATGGCCGGCAGCGTCGAGAGCCTGAACGTGTCGGTGGCGAGCGGCGTGTGCCTGTACGAAGCCGTG
CGCCAGCGGCGCGTGGCGGCCGCCGCGAAGTAA

Upstream 100 bases:

>100_bases
CGCGTTGTCCGGCTGCGAGGCCGGCAACGCGGCGCTTCCGTTTGCAATGCTCGCGAGGCGCGCCGGCCGCGCCCCGCGTC
AAGACGAAGGTTGCTCAGTC

Downstream 100 bases:

>100_bases
GCCGCAGGAGGCCGTGTTGATGAAAACGAGGCGCTGGCCGATCGCACGCCGGCCGGAGAGGATGACGGCGGGTAGCGCCG
GCCGGCGCGGCGGTGTGCAC

Product: TrmH family RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSRLKVLYGFHAVTARLRHDASTVAEVLYDQTRRDRRMTDFLQTAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDLP
LAQNLAELLDGIQGPALLLVLDGVTDPHNLGACLRVADAAGAHAIIAPRDRAVGLNATAAKVASGAADTVPYITVTNLAR
ALRELKDAGVWVIGTADDAPASLYETKLDGPVALVMGAEGEGMRRLTRDTCDDVMNLPMAGSVESLNVSVASGVCLYEAV
RQRRVAAAAK

Sequences:

>Translated_250_residues
MSRLKVLYGFHAVTARLRHDASTVAEVLYDQTRRDRRMTDFLQTAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDLP
LAQNLAELLDGIQGPALLLVLDGVTDPHNLGACLRVADAAGAHAIIAPRDRAVGLNATAAKVASGAADTVPYITVTNLAR
ALRELKDAGVWVIGTADDAPASLYETKLDGPVALVMGAEGEGMRRLTRDTCDDVMNLPMAGSVESLNVSVASGVCLYEAV
RQRRVAAAAK
>Mature_249_residues
SRLKVLYGFHAVTARLRHDASTVAEVLYDQTRRDRRMTDFLQTAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDLPL
AQNLAELLDGIQGPALLLVLDGVTDPHNLGACLRVADAAGAHAIIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARA
LRELKDAGVWVIGTADDAPASLYETKLDGPVALVMGAEGEGMRRLTRDTCDDVMNLPMAGSVESLNVSVASGVCLYEAVR
QRRVAAAAK

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=262, Percent_Identity=28.2442748091603, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1790623, Length=241, Percent_Identity=51.8672199170125, Blast_Score=247, Evalue=6e-67,
Organism=Escherichia coli, GI1788935, Length=189, Percent_Identity=30.1587301587302, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790083, Length=155, Percent_Identity=34.8387096774194, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17555908, Length=256, Percent_Identity=25.390625, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324775, Length=184, Percent_Identity=28.804347826087, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26627; Mature: 26496

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLKVLYGFHAVTARLRHDASTVAEVLYDQTRRDRRMTDFLQTAKEAGVRLIAADETRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARVEDLPLAQNLAELLDGIQGPALLLVLDGVTDPHNLGACLRVADAA
ECCHHHHHHCCHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCC
GAHAIIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARALRELKDAGVWVIGTADDAP
CCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCC
ASLYETKLDGPVALVMGAEGEGMRRLTRDTCDDVMNLPMAGSVESLNVSVASGVCLYEAV
HHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHCEEHHHCCHHHHHHH
RQRRVAAAAK
HHHHHHHCCC
>Mature Secondary Structure 
SRLKVLYGFHAVTARLRHDASTVAEVLYDQTRRDRRMTDFLQTAKEAGVRLIAADETRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARVEDLPLAQNLAELLDGIQGPALLLVLDGVTDPHNLGACLRVADAA
ECCHHHHHHCCHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCC
GAHAIIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARALRELKDAGVWVIGTADDAP
CCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCC
ASLYETKLDGPVALVMGAEGEGMRRLTRDTCDDVMNLPMAGSVESLNVSVASGVCLYEAV
HHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHCEEHHHCCHHHHHHH
RQRRVAAAAK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]