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The map label for this gene is amiD [H]

Identifier: 238027542

GI number: 238027542

Start: 2139850

End: 2140773

Strand: Reverse

Name: amiD [H]

Synonym: bglu_1g19600

Alternate gene names: 238027542

Gene position: 2140773-2139850 (Counterclockwise)

Preceding gene: 238027543

Following gene: 238027541

Centisome position: 54.8

GC content: 73.38

Gene sequence:

>924_bases
ATGACGGCGGGTAGCGCCGGCCGGCGCGGCGGTGTGCACGGTGCGCGCGTGCTGGCCGCGGCCTGCATCGCGGCGCTGCT
GAGCGCCTGCAGCGCGCCCACGCTCGTCGATCGCGGCAGCTATCTCGCCGATACGGGCCATCCGGCGCGCGGCGCCGATT
CGCGCGTGCGCTTTCTCGTGCTGCACTACACCGAGTCGGACGACCAGCGCGCGCGGTTCGTGCTGACCCACGACGAGGTC
AGCGCCCATTACCTGGTTCCGAGCCATCCGTCCTACCGCGACGGCAAGCCCGTCGTGCTGCAACTGGTGCCGGAAAGCGA
GCGTGCCTGGCACGCGGGCGTCAGCGACTGGCAGGGTACGACCGAGCTCAACGCGGCATCGATCGGCATCGAGATCGTCA
ATGCCGGCCCGCTCGACGCGCCGGCGAACCGGACCTGGCAGGCCTATCCGCCCGATCAGGTCGAGGCGGTGGTGAAGCTC
GCGCAGGACATCGTCGCGCGCTACCGGATACCGCCGACGCGCGTGGTCGCGCACAGCGACATCGCTCCCCAGCGCAAGAT
CGATCCCGGTCCGGCCTTCCCTTGGCAGGCGCTCGCGCGCGCCGGCGTCGGCGCCTGGCCCGACGACGCCACCGTGGCCG
CGCGGCTGGCGGGGCGCGCGCCCGGCGACCCGGCCGAGGTGCGCGGGCTGCAGCTGAAGCTCGCGCGCTACGGCTACGAC
GTGGCGACCGACGGCGTGCTCGACGCGCGCACGCGGCGCGTGTTCGCGGCGTTCCAGATGCATTTCCGGCCGGCCGACTA
CTCGGGCAATCCGGACGCGCAGACCGACGCGATCGCGCAGGCCCTGCTCGACAAGTATTTCCCCGATGCGGGCGTGCCGA
TCACGGCGCCGGCCGCCGCGGCCGGCCACGGCACGACGCCGTAG

Upstream 100 bases:

>100_bases
GCCGTGCGCCAGCGGCGCGTGGCGGCCGCCGCGAAGTAAGCCGCAGGAGGCCGTGTTGATGAAAACGAGGCGCTGGCCGA
TCGCACGCCGGCCGGAGAGG

Downstream 100 bases:

>100_bases
TTTCCCCGCGTTTCCGCTCGTTTCCGGTGCCGCCGCACGGCCGGGCGGCGGCGGATCCCCGTGGCCGTCCCGCTTGCCAT
CGGGCGCTCCGCGCGTCCGG

Product: N-acetylmuramyl-L-alanine amidase, negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTAGSAGRRGGVHGARVLAAACIAALLSACSAPTLVDRGSYLADTGHPARGADSRVRFLVLHYTESDDQRARFVLTHDEV
SAHYLVPSHPSYRDGKPVVLQLVPESERAWHAGVSDWQGTTELNAASIGIEIVNAGPLDAPANRTWQAYPPDQVEAVVKL
AQDIVARYRIPPTRVVAHSDIAPQRKIDPGPAFPWQALARAGVGAWPDDATVAARLAGRAPGDPAEVRGLQLKLARYGYD
VATDGVLDARTRRVFAAFQMHFRPADYSGNPDAQTDAIAQALLDKYFPDAGVPITAPAAAAGHGTTP

Sequences:

>Translated_307_residues
MTAGSAGRRGGVHGARVLAAACIAALLSACSAPTLVDRGSYLADTGHPARGADSRVRFLVLHYTESDDQRARFVLTHDEV
SAHYLVPSHPSYRDGKPVVLQLVPESERAWHAGVSDWQGTTELNAASIGIEIVNAGPLDAPANRTWQAYPPDQVEAVVKL
AQDIVARYRIPPTRVVAHSDIAPQRKIDPGPAFPWQALARAGVGAWPDDATVAARLAGRAPGDPAEVRGLQLKLARYGYD
VATDGVLDARTRRVFAAFQMHFRPADYSGNPDAQTDAIAQALLDKYFPDAGVPITAPAAAAGHGTTP
>Mature_306_residues
TAGSAGRRGGVHGARVLAAACIAALLSACSAPTLVDRGSYLADTGHPARGADSRVRFLVLHYTESDDQRARFVLTHDEVS
AHYLVPSHPSYRDGKPVVLQLVPESERAWHAGVSDWQGTTELNAASIGIEIVNAGPLDAPANRTWQAYPPDQVEAVVKLA
QDIVARYRIPPTRVVAHSDIAPQRKIDPGPAFPWQALARAGVGAWPDDATVAARLAGRAPGDPAEVRGLQLKLARYGYDV
ATDGVLDARTRRVFAAFQMHFRPADYSGNPDAQTDAIAQALLDKYFPDAGVPITAPAAAAGHGTTP

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=255, Percent_Identity=51.7647058823529, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1786300, Length=121, Percent_Identity=38.8429752066116, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 32585; Mature: 32454

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGSAGRRGGVHGARVLAAACIAALLSACSAPTLVDRGSYLADTGHPARGADSRVRFLV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCEEECCCCCCCCCCCCEEEEE
LHYTESDDQRARFVLTHDEVSAHYLVPSHPSYRDGKPVVLQLVPESERAWHAGVSDWQGT
EEECCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCEEEEEECCCCCHHHCCCCCCCCC
TELNAASIGIEIVNAGPLDAPANRTWQAYPPDQVEAVVKLAQDIVARYRIPPTRVVAHSD
CCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHEEECCC
IAPQRKIDPGPAFPWQALARAGVGAWPDDATVAARLAGRAPGDPAEVRGLQLKLARYGYD
CCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHEEEEEEHHCCC
VATDGVLDARTRRVFAAFQMHFRPADYSGNPDAQTDAIAQALLDKYFPDAGVPITAPAAA
CCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
AGHGTTP
CCCCCCC
>Mature Secondary Structure 
TAGSAGRRGGVHGARVLAAACIAALLSACSAPTLVDRGSYLADTGHPARGADSRVRFLV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCEEECCCCCCCCCCCCEEEEE
LHYTESDDQRARFVLTHDEVSAHYLVPSHPSYRDGKPVVLQLVPESERAWHAGVSDWQGT
EEECCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCEEEEEECCCCCHHHCCCCCCCCC
TELNAASIGIEIVNAGPLDAPANRTWQAYPPDQVEAVVKLAQDIVARYRIPPTRVVAHSD
CCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHEEECCC
IAPQRKIDPGPAFPWQALARAGVGAWPDDATVAARLAGRAPGDPAEVRGLQLKLARYGYD
CCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHEEEEEEHHCCC
VATDGVLDARTRRVFAAFQMHFRPADYSGNPDAQTDAIAQALLDKYFPDAGVPITAPAAA
CCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
AGHGTTP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]