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The map label for this gene is dmlR [H]

Identifier: 238027211

GI number: 238027211

Start: 1747027

End: 1748019

Strand: Reverse

Name: dmlR [H]

Synonym: bglu_1g15970

Alternate gene names: 238027211

Gene position: 1748019-1747027 (Counterclockwise)

Preceding gene: 238027214

Following gene: 238027210

Centisome position: 44.75

GC content: 74.42

Gene sequence:

>993_bases
ATGAGCTCGACCCCGCCCTCGGATGACGTGCCGCCCTCCGCCCCCGACAAGCCGCTCGACCTGCTCGACGTGGCGCTGTT
CGTGCGCGCCGCGCTGCTCGCGAACCTCTCGGCGGCCGGGCGCGAGTTCGGCGTCTCGGCCACGGTCGCGAGCACGCGGC
TCGCGCAGCTCGAGCGCCAGCTCGGCGCGCGGCTGCTGCATCGCACGACGCGGCGCGTGACGCCGACCCAGGACGGCGAA
CTGTTCCTGGTCCGCGCGCAGGCGCTGCTCGACGCAGCCGACGCGGCGCGCGCGGCGGTCGGGCACGCGCGCCGCGAGCC
GCACGGGCGGCTGCGCGTGTCGATGTCGTCGTCATTCGGGCGGCAGCACGTCGCGCCCGTGATCCCCGCGTTCCTGCGCC
GCTACCCGCAGGTGTCGATCGACCTGCGGCTGTCCGACGAGCTCGTCGACCTCGTCGACGACGGCATCGACGTCGCGATC
CGGATCGGCGCGCTGCGCGACTCGTCGCTGGTGGCGCGCCGGCTCGCGGTGAACCGGCGCGTGGTATGCGCCTCGCCCGC
CTATCTGGCCGCGCACGGCACGCCGCGTCATCCGGCCGAGCTCGCGCGCCACCAATGCGTGATCCTCGGCGACCAGCGCG
ACTGGGCCTTCGAGACGCCGGCCGGGCCGCTCACCGTGCGCGTGGGCGGGCGGCTGGTGGCCAGCAACGGCGAGGCGATC
CGCGAGGCGCTGGTGGACGGCTTCGGCATCGCGATCAAATCCACCTGGGACGTCGGGCCGAACCTGCGCGACGGCTCGCT
GGTGACGGTGCTCGCCGACTATCCGCTCGCCGAGCCCTCGGCGATCTGGGCCGTCTATCCGAGCCGCGCCTTCGTGCCGC
TGAAGCTGCACGCCTTCATCGATTTCCTCGCCGCGCATCTGGGCGACCCGCCGTACTGGGATCGCGCGCACGAGCGCATG
CGCGCGACGGCCGCCGATCCCGGCCTCGCCTGA

Upstream 100 bases:

>100_bases
TCGGGGGCAATCGGGGTTGGTGACGGGTCCGATCGTAAGCGGCCCGATCCTGAAGAAAAAGCGACATAATCCCGGAAACA
TTTTTTGGAATTTCCGGGTA

Downstream 100 bases:

>100_bases
GGGCCCGCGCGCGCCGACCGGCTACAATAGCGACCTCTTGCGGCGCGCCTGGCGCGCCGTTTCCGCCCCACGCGACCCGT
CATGACCACGAATCTCGTCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSSTPPSDDVPPSAPDKPLDLLDVALFVRAALLANLSAAGREFGVSATVASTRLAQLERQLGARLLHRTTRRVTPTQDGE
LFLVRAQALLDAADAARAAVGHARREPHGRLRVSMSSSFGRQHVAPVIPAFLRRYPQVSIDLRLSDELVDLVDDGIDVAI
RIGALRDSSLVARRLAVNRRVVCASPAYLAAHGTPRHPAELARHQCVILGDQRDWAFETPAGPLTVRVGGRLVASNGEAI
REALVDGFGIAIKSTWDVGPNLRDGSLVTVLADYPLAEPSAIWAVYPSRAFVPLKLHAFIDFLAAHLGDPPYWDRAHERM
RATAADPGLA

Sequences:

>Translated_330_residues
MSSTPPSDDVPPSAPDKPLDLLDVALFVRAALLANLSAAGREFGVSATVASTRLAQLERQLGARLLHRTTRRVTPTQDGE
LFLVRAQALLDAADAARAAVGHARREPHGRLRVSMSSSFGRQHVAPVIPAFLRRYPQVSIDLRLSDELVDLVDDGIDVAI
RIGALRDSSLVARRLAVNRRVVCASPAYLAAHGTPRHPAELARHQCVILGDQRDWAFETPAGPLTVRVGGRLVASNGEAI
REALVDGFGIAIKSTWDVGPNLRDGSLVTVLADYPLAEPSAIWAVYPSRAFVPLKLHAFIDFLAAHLGDPPYWDRAHERM
RATAADPGLA
>Mature_329_residues
SSTPPSDDVPPSAPDKPLDLLDVALFVRAALLANLSAAGREFGVSATVASTRLAQLERQLGARLLHRTTRRVTPTQDGEL
FLVRAQALLDAADAARAAVGHARREPHGRLRVSMSSSFGRQHVAPVIPAFLRRYPQVSIDLRLSDELVDLVDDGIDVAIR
IGALRDSSLVARRLAVNRRVVCASPAYLAAHGTPRHPAELARHQCVILGDQRDWAFETPAGPLTVRVGGRLVASNGEAIR
EALVDGFGIAIKSTWDVGPNLRDGSLVTVLADYPLAEPSAIWAVYPSRAFVPLKLHAFIDFLAAHLGDPPYWDRAHERMR
ATAADPGLA

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=294, Percent_Identity=39.1156462585034, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1789440, Length=297, Percent_Identity=33.3333333333333, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=32.7645051194539, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=29.4520547945205, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789639, Length=287, Percent_Identity=29.9651567944251, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=31.3588850174216, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=26.2068965517241, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789173, Length=274, Percent_Identity=23.7226277372263, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786448, Length=261, Percent_Identity=27.9693486590038, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788706, Length=190, Percent_Identity=26.3157894736842, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788481, Length=295, Percent_Identity=24.0677966101695, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35595; Mature: 35464

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTPPSDDVPPSAPDKPLDLLDVALFVRAALLANLSAAGREFGVSATVASTRLAQLERQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHH
LGARLLHRTTRRVTPTQDGELFLVRAQALLDAADAARAAVGHARREPHGRLRVSMSSSFG
HHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
RQHVAPVIPAFLRRYPQVSIDLRLSDELVDLVDDGIDVAIRIGALRDSSLVARRLAVNRR
HHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCE
VVCASPAYLAAHGTPRHPAELARHQCVILGDQRDWAFETPAGPLTVRVGGRLVASNGEAI
EEEECCCEEEECCCCCCHHHHHHCEEEEEECCCCCCEECCCCCEEEEECCEEEECCCHHH
REALVDGFGIAIKSTWDVGPNLRDGSLVTVLADYPLAEPSAIWAVYPSRAFVPLKLHAFI
HHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCEEEEHHHHHH
DFLAAHLGDPPYWDRAHERMRATAADPGLA
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSTPPSDDVPPSAPDKPLDLLDVALFVRAALLANLSAAGREFGVSATVASTRLAQLERQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHH
LGARLLHRTTRRVTPTQDGELFLVRAQALLDAADAARAAVGHARREPHGRLRVSMSSSFG
HHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
RQHVAPVIPAFLRRYPQVSIDLRLSDELVDLVDDGIDVAIRIGALRDSSLVARRLAVNRR
HHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCE
VVCASPAYLAAHGTPRHPAELARHQCVILGDQRDWAFETPAGPLTVRVGGRLVASNGEAI
EEEECCCEEEECCCCCCHHHHHHCEEEEEECCCCCCEECCCCCEEEEECCEEEECCCHHH
REALVDGFGIAIKSTWDVGPNLRDGSLVTVLADYPLAEPSAIWAVYPSRAFVPLKLHAFI
HHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCEEEEHHHHHH
DFLAAHLGDPPYWDRAHERMRATAADPGLA
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]