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The map label for this gene is gcl [H]

Identifier: 238027014

GI number: 238027014

Start: 1533525

End: 1535300

Strand: Reverse

Name: gcl [H]

Synonym: bglu_1g13920

Alternate gene names: 238027014

Gene position: 1535300-1533525 (Counterclockwise)

Preceding gene: 238027023

Following gene: 238027013

Centisome position: 39.3

GC content: 69.59

Gene sequence:

>1776_bases
ATGGCCAAGATGAGAGCCGTCGACGCCGCCGTGCTGGTGCTGGAGAAGGAAGGCATCCAGACCGCCTTCGGCGTGCCGGG
CGCCGCGATCAACCCGTTCTACTCGGCGCTGCGCAAGTCGGGCGCGATCGGCCACGTGCTGGCGCGCCACGTCGAGGGCG
CCTCGCACATGGCCGAGGGCTACACGCGCGCCGCGCCGGGCAACATCGGCGTATGCATCGGCACCTCGGGGCCGGCCGGC
ACCGACATGATCACCGGCCTCTATTCGGCCTGGGCCGATTCGATCCCGATCCTCGCGATCACCGGCCAGGCGCCGCGCGC
GCGGCTCTACAAGGAGGACTTCCAGGCCGTCGACATCGAATCGATCGCCAAGCCGGTCACCAAGTGGGCCGTGACGGTGC
GCGAGCCGGCCCTCGTGCCGCGCGTGTTCCAGCAGGCCTTCCACCTGATGCGCTCGGGCCGCCCCGGCCCGGTGCTGATC
GACCTGCCGATCGACGTGCAGCTCGCCGAGATCGAGTTCGATATCGACACCTACGCGCCGCTGCCGGCCTACAAGCCGGC
CGCCACGCGCGCCCAGATCGAGGCCGCGCTTACGATGCTGGACGAGGCCGAGCGGCCGCTGATCGTGTCGGGCGGCGGCG
TGCTGAACGCGGCGGCCGAGGATCTGCTGGTCGAGTTCGCCGAGCTGCTCGGGGTGCCGGTGATCCCGACGCTGATGTCG
TGGGGCGCGATTCCCGACGATCACCCGCTGATGGCCGGCATGGTCGGCTTGCAGACCTCGCACCGCTACGGCAACGCCAC
GCTGCTCGCCTCGGACTTCGTGCTCGGCATCGGCAACCGCTGGGCCAACCGCCACACCGGCAGCGTCGAGGTCTACACGA
AGGGCCGCAGGTTCGTCCACGTCGACATCGAGCCGACCCAGATCGGCCGCGTGTTCGGCCCCGACCTCGGCATCGTGTCC
GATGCGAAGGCCGCGCTCGAGCTGTTCGTGGCGGCGGCCCGCGAATGGAAGGCGGCCGGACGGCTCAAGGACCGCGGCGC
CTGGGTGGCGGACTGCCAGCAGCGCAAGCGCACGCTGCAGCGCAAGACCCATTTCGACGACGTGCCGGTCAAGCCGCAGC
GGGTCTACGAGGAGATGAACAAGGCGTTCGGCCGCAACACCTGCTACGTCAGCACGATCGGCCTGTCGCAAATCGCCGCC
GCGCAGTTCCTGCACGTCTATCAGGCGCGCAACTGGATCAACTGCGGGCAGGCAGGCCCGCTCGGCTGGACCATTCCGGC
CGCGCTCGGCGTGCGTGCCGCGGACCCGGGCCGGCCGATCGTCGCGCTGTCGGGCGACTACGACTTCCAGTTCATGATCG
AGGAGCTCGCGGTGGGCGCGCAGTTCAAGCTGCCCTATGTGCACGTGGTGGTGAACAACGCGTATCTGGGCCTGATCCGT
CAGGCACAGCGCGCGTTCGACATGGACTACTGCGTGCAGCTCGCGTTCGAGAACGTCAACGCGCCCGAGCTCGGCAGCTA
TGGCGTGGACCACGTCGCCGTGGCCGAAGGGCTCGGCTGCAAGGCCCTGCGCGTGACGAGGCCCGAGCAGATCGCGCCCG
CCTTCGAGCAGGCCAAGCAGCTGATCGCCGAGTTCGGCGTGCCGGTGGTGGTGGAGGTGATCCTCGAGCGCGTGACCCAT
ATCGCGATGGGCGCCGAGCTCGACGCGGTCAACGAGTTCGAGCCGCTCGCCGAGCGCCCCGAACACGCGCCGACCGCGAT
CTCGCTGCTCGACTGA

Upstream 100 bases:

>100_bases
TTAGTTTCGAAATAAGCGACGAGCGGCGATCTTATCAAAGCTTTCATGCCGACCTACAGTGACGACGACAACGTGATGCG
CGATTTCCAAGGAGACATTC

Downstream 100 bases:

>100_bases
GCCTGCTGCTTGCCGACCGACCGGAGACCCCGCCATGCCGAAATTCGCCGCCAACCTGACGATGCTGTTCAACGAAGTGC
CGTTCCTCGAGCGGTTCGAG

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MAKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSALRKSGAIGHVLARHVEGASHMAEGYTRAAPGNIGVCIGTSGPAG
TDMITGLYSAWADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLI
DLPIDVQLAEIEFDIDTYAPLPAYKPAATRAQIEAALTMLDEAERPLIVSGGGVLNAAAEDLLVEFAELLGVPVIPTLMS
WGAIPDDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSVEVYTKGRRFVHVDIEPTQIGRVFGPDLGIVS
DAKAALELFVAAAREWKAAGRLKDRGAWVADCQQRKRTLQRKTHFDDVPVKPQRVYEEMNKAFGRNTCYVSTIGLSQIAA
AQFLHVYQARNWINCGQAGPLGWTIPAALGVRAADPGRPIVALSGDYDFQFMIEELAVGAQFKLPYVHVVVNNAYLGLIR
QAQRAFDMDYCVQLAFENVNAPELGSYGVDHVAVAEGLGCKALRVTRPEQIAPAFEQAKQLIAEFGVPVVVEVILERVTH
IAMGAELDAVNEFEPLAERPEHAPTAISLLD

Sequences:

>Translated_591_residues
MAKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSALRKSGAIGHVLARHVEGASHMAEGYTRAAPGNIGVCIGTSGPAG
TDMITGLYSAWADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLI
DLPIDVQLAEIEFDIDTYAPLPAYKPAATRAQIEAALTMLDEAERPLIVSGGGVLNAAAEDLLVEFAELLGVPVIPTLMS
WGAIPDDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSVEVYTKGRRFVHVDIEPTQIGRVFGPDLGIVS
DAKAALELFVAAAREWKAAGRLKDRGAWVADCQQRKRTLQRKTHFDDVPVKPQRVYEEMNKAFGRNTCYVSTIGLSQIAA
AQFLHVYQARNWINCGQAGPLGWTIPAALGVRAADPGRPIVALSGDYDFQFMIEELAVGAQFKLPYVHVVVNNAYLGLIR
QAQRAFDMDYCVQLAFENVNAPELGSYGVDHVAVAEGLGCKALRVTRPEQIAPAFEQAKQLIAEFGVPVVVEVILERVTH
IAMGAELDAVNEFEPLAERPEHAPTAISLLD
>Mature_590_residues
AKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSALRKSGAIGHVLARHVEGASHMAEGYTRAAPGNIGVCIGTSGPAGT
DMITGLYSAWADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLID
LPIDVQLAEIEFDIDTYAPLPAYKPAATRAQIEAALTMLDEAERPLIVSGGGVLNAAAEDLLVEFAELLGVPVIPTLMSW
GAIPDDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSVEVYTKGRRFVHVDIEPTQIGRVFGPDLGIVSD
AKAALELFVAAAREWKAAGRLKDRGAWVADCQQRKRTLQRKTHFDDVPVKPQRVYEEMNKAFGRNTCYVSTIGLSQIAAA
QFLHVYQARNWINCGQAGPLGWTIPAALGVRAADPGRPIVALSGDYDFQFMIEELAVGAQFKLPYVHVVVNNAYLGLIRQ
AQRAFDMDYCVQLAFENVNAPELGSYGVDHVAVAEGLGCKALRVTRPEQIAPAFEQAKQLIAEFGVPVVVEVILERVTHI
AMGAELDAVNEFEPLAERPEHAPTAISLLD

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=22.7758007117438, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI21361361, Length=598, Percent_Identity=24.5819397993311, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1786717, Length=590, Percent_Identity=78.8135593220339, Blast_Score=966, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=567, Percent_Identity=32.0987654320988, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1790104, Length=479, Percent_Identity=33.8204592901879, Blast_Score=261, Evalue=9e-71,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=24.3194192377495, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1788716, Length=477, Percent_Identity=26.4150943396226, Blast_Score=138, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=551, Percent_Identity=25.0453720508167, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17531299, Length=516, Percent_Identity=22.6744186046512, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17531301, Length=516, Percent_Identity=22.6744186046512, Blast_Score=92, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=596, Percent_Identity=29.8657718120805, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6320816, Length=564, Percent_Identity=24.645390070922, Blast_Score=106, Evalue=8e-24,
Organism=Drosophila melanogaster, GI19922626, Length=572, Percent_Identity=25.6993006993007, Blast_Score=147, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 63904; Mature: 63773

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSALRKSGAIGHVLARHVEGASHMAEG
CCCCHHHCEEEEEEECCCCCHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHC
YTRAAPGNIGVCIGTSGPAGTDMITGLYSAWADSIPILAITGQAPRARLYKEDFQAVDIE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLIDLPIDVQLAEIEFDIDTYAP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCC
LPAYKPAATRAQIEAALTMLDEAERPLIVSGGGVLNAAAEDLLVEFAELLGVPVIPTLMS
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
WGAIPDDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSVEVYTKGRRFVH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALELFVAAAREWKAAGRLKDRGAWVADCQQRKRTLQ
EECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
RKTHFDDVPVKPQRVYEEMNKAFGRNTCYVSTIGLSQIAAAQFLHVYQARNWINCGQAGP
HHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
LGWTIPAALGVRAADPGRPIVALSGDYDFQFMIEELAVGAQFKLPYVHVVVNNAYLGLIR
CCEEHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCEECCCEEEEEECCHHHHHHH
QAQRAFDMDYCVQLAFENVNAPELGSYGVDHVAVAEGLGCKALRVTRPEQIAPAFEQAKQ
HHHHHHCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCHHHHHHHHHHHH
LIAEFGVPVVVEVILERVTHIAMGAELDAVNEFEPLAERPEHAPTAISLLD
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCEEEECC
>Mature Secondary Structure 
AKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSALRKSGAIGHVLARHVEGASHMAEG
CCCHHHCEEEEEEECCCCCHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHC
YTRAAPGNIGVCIGTSGPAGTDMITGLYSAWADSIPILAITGQAPRARLYKEDFQAVDIE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLIDLPIDVQLAEIEFDIDTYAP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCC
LPAYKPAATRAQIEAALTMLDEAERPLIVSGGGVLNAAAEDLLVEFAELLGVPVIPTLMS
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
WGAIPDDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSVEVYTKGRRFVH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALELFVAAAREWKAAGRLKDRGAWVADCQQRKRTLQ
EECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
RKTHFDDVPVKPQRVYEEMNKAFGRNTCYVSTIGLSQIAAAQFLHVYQARNWINCGQAGP
HHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
LGWTIPAALGVRAADPGRPIVALSGDYDFQFMIEELAVGAQFKLPYVHVVVNNAYLGLIR
CCEEHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCEECCCEEEEEECCHHHHHHH
QAQRAFDMDYCVQLAFENVNAPELGSYGVDHVAVAEGLGCKALRVTRPEQIAPAFEQAKQ
HHHHHHCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCHHHHHHHHHHHH
LIAEFGVPVVVEVILERVTHIAMGAELDAVNEFEPLAERPEHAPTAISLLD
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]