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The map label for this gene is pncA [H]

Identifier: 238026994

GI number: 238026994

Start: 1514247

End: 1514873

Strand: Direct

Name: pncA [H]

Synonym: bglu_1g13720

Alternate gene names: 238026994

Gene position: 1514247-1514873 (Clockwise)

Preceding gene: 238026993

Following gene: 238026995

Centisome position: 38.76

GC content: 71.93

Gene sequence:

>627_bases
ATGATGCGGACCGACGACGTGCTGCTCGTGATCGACGTGCAGAACGATTTCATGCCGGGCGGCGCGCTCGCGGTGGCCGA
CGGCGACGCGGTGGTGCCGGTGATCAACCGGCTCGCGCGGCGCTTCGATCAGGTGGTGATCACGCAGGACTGGCATCCGC
GCGCGCACGTGTCGTTTGCCGCCAACCACGCGGGCCGCGAGCCGTTCTCGACGCTGACGCTGCCCTACGGCGAGCAGGTG
CTGTGGCCCGTGCATTGCGTGCAGGACACCGACGGCGCCGCGCTGCACCGCGATCTCGACGTCCCCCATGCGCGGCTCGT
GATCCGCAAGGGGCTCGACCCGGCCGTCGACAGCTATTCGGCCTTCGTCGAGGCCGACCGTGCGACTTCCACCGGCCTCG
CCGCCTATCTGCAGGCGCTCGGCGCCAAGCGCGTCTGGTGCTGCGGGCTCGCCACCGACTATTGCGTCGCGTGGTCCGCG
CTCGATGCACGCGCGGCCGGCTTCGAGGCCGCGGTGATCGAGGACGCGTGCCGCGCGATCGACCTCGACGGCTCGCTCGC
GCGCGCCTGGCAGGACCTTCACGGCGCGGGCGTGGCCCGCGTGCATGCGGACGCGGTGCCGCTCTGA

Upstream 100 bases:

>100_bases
CGCGGCCGGCGCGAACGGCGACGCGTGGCGGATCGCGCCGTCGATCGTCGAGCTGGCCGCCAGCGGCAAGGGTTTCAACG
GCTGACGCGCGGAGGACACG

Downstream 100 bases:

>100_bases
GCCCGCCGGCGCGCGCCGCGTTCCGTCTACAAGCAATTCGAACCAGGAGACTGGCATGACCGAAGCCGTAATCGTATCCA
CCGCCCGCACCGCGCTTGCC

Product: Nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MMRTDDVLLVIDVQNDFMPGGALAVADGDAVVPVINRLARRFDQVVITQDWHPRAHVSFAANHAGREPFSTLTLPYGEQV
LWPVHCVQDTDGAALHRDLDVPHARLVIRKGLDPAVDSYSAFVEADRATSTGLAAYLQALGAKRVWCCGLATDYCVAWSA
LDARAAGFEAAVIEDACRAIDLDGSLARAWQDLHGAGVARVHADAVPL

Sequences:

>Translated_208_residues
MMRTDDVLLVIDVQNDFMPGGALAVADGDAVVPVINRLARRFDQVVITQDWHPRAHVSFAANHAGREPFSTLTLPYGEQV
LWPVHCVQDTDGAALHRDLDVPHARLVIRKGLDPAVDSYSAFVEADRATSTGLAAYLQALGAKRVWCCGLATDYCVAWSA
LDARAAGFEAAVIEDACRAIDLDGSLARAWQDLHGAGVARVHADAVPL
>Mature_208_residues
MMRTDDVLLVIDVQNDFMPGGALAVADGDAVVPVINRLARRFDQVVITQDWHPRAHVSFAANHAGREPFSTLTLPYGEQV
LWPVHCVQDTDGAALHRDLDVPHARLVIRKGLDPAVDSYSAFVEADRATSTGLAAYLQALGAKRVWCCGLATDYCVAWSA
LDARAAGFEAAVIEDACRAIDLDGSLARAWQDLHGAGVARVHADAVPL

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=195, Percent_Identity=43.0769230769231, Blast_Score=147, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI193206735, Length=221, Percent_Identity=34.3891402714932, Blast_Score=106, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17543130, Length=221, Percent_Identity=34.3891402714932, Blast_Score=106, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI71999339, Length=197, Percent_Identity=32.994923857868, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321401, Length=193, Percent_Identity=35.2331606217617, Blast_Score=116, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24648247, Length=205, Percent_Identity=38.0487804878049, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22379; Mature: 22379

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRTDDVLLVIDVQNDFMPGGALAVADGDAVVPVINRLARRFDQVVITQDWHPRAHVSFA
CCCCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHEEEEECCCCCCEEEEEE
ANHAGREPFSTLTLPYGEQVLWPVHCVQDTDGAALHRDLDVPHARLVIRKGLDPAVDSYS
CCCCCCCCCCEEECCCCCCEEEEEEEECCCCCCEEEECCCCCHHHHHHHCCCCHHHHHHH
AFVEADRATSTGLAAYLQALGAKRVWCCGLATDYCVAWSALDARAAGFEAAVIEDACRAI
HHHHHCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHEE
DLDGSLARAWQDLHGAGVARVHADAVPL
CCCCHHHHHHHHHHCCCEEEEECCCCCC
>Mature Secondary Structure
MMRTDDVLLVIDVQNDFMPGGALAVADGDAVVPVINRLARRFDQVVITQDWHPRAHVSFA
CCCCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHEEEEECCCCCCEEEEEE
ANHAGREPFSTLTLPYGEQVLWPVHCVQDTDGAALHRDLDVPHARLVIRKGLDPAVDSYS
CCCCCCCCCCEEECCCCCCEEEEEEEECCCCCCEEEECCCCCHHHHHHHCCCCHHHHHHH
AFVEADRATSTGLAAYLQALGAKRVWCCGLATDYCVAWSALDARAAGFEAAVIEDACRAI
HHHHHCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHEE
DLDGSLARAWQDLHGAGVARVHADAVPL
CCCCHHHHHHHHHHCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]