Click here to switch to the map view.

The map label for this gene is ispF [H]

Identifier: 238026958

GI number: 238026958

Start: 1461909

End: 1462394

Strand: Direct

Name: ispF [H]

Synonym: bglu_1g13340

Alternate gene names: 238026958

Gene position: 1461909-1462394 (Clockwise)

Preceding gene: 238026957

Following gene: 238026961

Centisome position: 37.42

GC content: 69.34

Gene sequence:

>486_bases
ATGGACATCAGAATCGGACAAGGCTATGACGTACACCAGCTGGTTCCCGGCCGGCCGCTCATCATCGGCGGCGTGACGAT
CCCCTACGAACGCGGCCTGCTCGGCCACTCGGACGCCGACGTGCTGCTGCACGCGCTGACCGACGCGCTGTTCGGCGCCG
CCGCGCTCGGCGACATCGGCCGGCACTTCTCGGACACCGATCCGCAGTTCAAGGGCGCCGACAGCCGCGTGCTGCTGCGC
GAATGCGCGAAGCGCGTGGCCGCCGCCGGCTTTGCGATCGGCAACGTCGATACCACCGTGATCGCACAGGCGCCGAAGCT
CGCGCCGCACATCGACGCGATGCGCGCGAACATTGCCGAGGATCTGGGGCTGCCGCTCGAGCGCGTCAACGTGAAGGCCA
AGACCAACGAGAAGCTCGGCTATCTCGGCCGCGGCGAAGGCATCGAGGCGCAGGCCGCCGCGCTGCTGGTGCGCCGCGCG
GCATAG

Upstream 100 bases:

>100_bases
TTCAAGGTCACCTATCCCGAGGATTTCGAACTGGCCGAGGCATTCCTGTCGCGGCCCGCGAACGCCTCCTGAACCCCCTT
CACCACGAACCAGGCACCGC

Downstream 100 bases:

>100_bases
GCGGCCGTCCCGCGCCTGCGGCCGAGGCCGCCCGCGGGCCGCCGCCGAAGCAAGATGACGCCGCGCGGGCTGCCCGCGCG
GCGGCGCCGCCCGGTACCGG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MDIRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHALTDALFGAAALGDIGRHFSDTDPQFKGADSRVLLR
ECAKRVAAAGFAIGNVDTTVIAQAPKLAPHIDAMRANIAEDLGLPLERVNVKAKTNEKLGYLGRGEGIEAQAAALLVRRA
A

Sequences:

>Translated_161_residues
MDIRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHALTDALFGAAALGDIGRHFSDTDPQFKGADSRVLLR
ECAKRVAAAGFAIGNVDTTVIAQAPKLAPHIDAMRANIAEDLGLPLERVNVKAKTNEKLGYLGRGEGIEAQAAALLVRRA
A
>Mature_161_residues
MDIRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHALTDALFGAAALGDIGRHFSDTDPQFKGADSRVLLR
ECAKRVAAAGFAIGNVDTTVIAQAPKLAPHIDAMRANIAEDLGLPLERVNVKAKTNEKLGYLGRGEGIEAQAAALLVRRA
A

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=66.025641025641, Blast_Score=197, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17010; Mature: 17010

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHALTDALFGAAALGDIG
CCCCCCCCCCHHHCCCCCCEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RHFSDTDPQFKGADSRVLLRECAKRVAAAGFAIGNVDTTVIAQAPKLAPHIDAMRANIAE
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEECCCCCEEEECCCCCCHHHHHHHHHHHH
DLGLPLERVNVKAKTNEKLGYLGRGEGIEAQAAALLVRRAA
HHCCCHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MDIRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHALTDALFGAAALGDIG
CCCCCCCCCCHHHCCCCCCEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RHFSDTDPQFKGADSRVLLRECAKRVAAAGFAIGNVDTTVIAQAPKLAPHIDAMRANIAE
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEECCCCCEEEECCCCCCHHHHHHHHHHHH
DLGLPLERVNVKAKTNEKLGYLGRGEGIEAQAAALLVRRAA
HHCCCHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA