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The map label for this gene is mscL [H]

Identifier: 238026950

GI number: 238026950

Start: 1448855

End: 1449298

Strand: Reverse

Name: mscL [H]

Synonym: bglu_1g13250

Alternate gene names: 238026950

Gene position: 1449298-1448855 (Counterclockwise)

Preceding gene: 238026951

Following gene: 238026949

Centisome position: 37.1

GC content: 60.36

Gene sequence:

>444_bases
ATGAGCATCATGAAGGAGTTTAAGGAGTTCGCCGTCAAGGGCAATGTGATGGATCTGGCCGTCGGCGTGATCATCGGCGG
CGCGTTTTCGAAGATCGTCGATTCCGTCGTCAAGGATCTCATCATGCCGGTGATCGGGGTGATCACCGGGGGCCTCGATT
TCTCGAACAAGTTCATACAGCTCGGCCCGATCCCGGCGACCTTCAAGGGCAATCCCGACTCGTACAAGGATCTGCAGGCC
GCAGGCGTCGCGCTGTTCGGCTACGGCTCGTTCATCACCGTGCTGATCAACTTCATCATCCTCGCGTTCATCATCTTTCT
GATGGTGCGCTTCATCAACCGGCTGCGCCGCCCGGTCGAAACCGCGCCGGCCGAACCGCCGCCGCCGCCCGAGGACGTGA
TGCTGCTGCGCGAGATCCGCGATTCGCTGAAGAACCGAGGCTGA

Upstream 100 bases:

>100_bases
GGGAAGTATCGGTGTCCGATCGCCTTCGCTGCCTGGGCGCGATCATCGCGCGGGCGAACCGGCCGCGCATTCTCTTTCCC
TTTTCTGGAGAAACCTGCTT

Downstream 100 bases:

>100_bases
CGCGCCCCGCCGCCATCGGATCGGCCCGCCGCGGGCCGGTCCCGAGCCCGCCGCGCGCGGGCTTTTTTGCGGCCTTTTTT
TGTGCGGCGCGATCGGGCGG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MSIMKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVITGGLDFSNKFIQLGPIPATFKGNPDSYKDLQA
AGVALFGYGSFITVLINFIILAFIIFLMVRFINRLRRPVETAPAEPPPPPEDVMLLREIRDSLKNRG

Sequences:

>Translated_147_residues
MSIMKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVITGGLDFSNKFIQLGPIPATFKGNPDSYKDLQA
AGVALFGYGSFITVLINFIILAFIIFLMVRFINRLRRPVETAPAEPPPPPEDVMLLREIRDSLKNRG
>Mature_146_residues
SIMKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVITGGLDFSNKFIQLGPIPATFKGNPDSYKDLQAA
GVALFGYGSFITVLINFIILAFIIFLMVRFINRLRRPVETAPAEPPPPPEDVMLLREIRDSLKNRG

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=147, Percent_Identity=46.9387755102041, Blast_Score=118, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 16098; Mature: 15967

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIMKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVITGGLDFSNKFIQ
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LGPIPATFKGNPDSYKDLQAAGVALFGYGSFITVLINFIILAFIIFLMVRFINRLRRPVE
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
TAPAEPPPPPEDVMLLREIRDSLKNRG
CCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIMKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVITGGLDFSNKFIQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LGPIPATFKGNPDSYKDLQAAGVALFGYGSFITVLINFIILAFIIFLMVRFINRLRRPVE
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
TAPAEPPPPPEDVMLLREIRDSLKNRG
CCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA