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The map label for this gene is rluF [H]

Identifier: 238026899

GI number: 238026899

Start: 1389901

End: 1391532

Strand: Reverse

Name: rluF [H]

Synonym: bglu_1g12680

Alternate gene names: 238026899

Gene position: 1391532-1389901 (Counterclockwise)

Preceding gene: 238026900

Following gene: 238026893

Centisome position: 35.62

GC content: 73.28

Gene sequence:

>1632_bases
ATGCGCACCAAACTGACCATCAAGCATCCTCGCCCGGCATCGCCGTCCCGCGCCCCGGTCCGCTCAGGCAGCCTCGTCGC
GCGCAAGCCGACGCGCCCCGCCGCGCCGCCCGATGGCGCGCCGAAGCCGAAGTCGAAGCCGAGCGCGAAAGGCGCGGCGC
CGCGCGCGGCCAAGCCGGCGGGCGCCGGCGCCGTGAAACCGCGCGCGCCGCGCGAGGCCGACCGGCCATCGCGCAGCGAA
GCCCGGCCCGCTCGCGACGGCGAGCGGCCGTTCCGCAGCGAAGGGCGACCTGCTCGTGACGGCGAACGCCCCGTCCGCAG
CGGTGCACGCCCGCCGCGCGACGGCGAGCGGCCGTTCCGCAGCGAAGGGCGACCTGCTCGTGACGGCGAGCGCCCCGCCC
GCAACGGCGCGCGTCCGCCGCGCGACGGCGAGCGGCCGTTCCGCAGCGAAGGGCGACCTGCTCGCGACGGCGAGCGCCCC
GTCCGCAGCGGCGCACGTCCGCCGCGCGACGGCGAGCGGCCGTTCCGCAGCGAAGGGCGACCTGCTCGTGACGGCGAACG
CCCCGTCCGCAGCGGTGCACGCCCGCCGCGCGACGGCGAGCGGCCGTTCCGCAGCGAAGGGCGACCTGCTCGCGATGGCG
AGCGCCCCTTGCGCGGCACAGCACGGCCCGTGCGCGACGGCGAGCGCCCGGTCCGCGCCGGTACACGCCCGCCGCGTGAC
GGCGATCGACCGTCGCGGGTCGAATCCCGCCCCGCTCGCGACACGGATCGCCCGGCCCGCAAGCCGCGCGTCACCACGGA
GCGTCCCGCGCCCTCGCGCCTGAAAGTCGCGCAGCCGGTCAAGGCACGTCCGTCGTTCGATTCCGGCGATGAAGCCGGGA
TGATGCGTCTGTCGAAGCGGATGTCGGAACTCGGCCTCTGCTCGCGGCGCGAGGCGGACGAATGGATCGAGAAGGGCTGG
GTGCTCGTCGATGGCGAACGGATCGACACGCTCGGCACCAAGGTGCGCCCGGACCAGAAGATCGAGATCGACGATCAGGC
GATCGCCGCCCAGGCCGCGCAGGTCACGATCCTGCTGCACAAGCCGGTCGGCTACGTGTCGGGTCAGGCCGAGGACGGCT
ACGAGCCGGCCGTGACGCTGATCACGCGCGAGAACCACTGGAGCGGCGACCGCTCCGCACTGCGTCTGTCGCCGCTGCAT
CTGCGCACGCTGGCGCCGGCCGGGCGGCTCGACATCGATTCGACCGGCTTGCTGGTGCTCACGCAGGACGGCCGCATCGC
CAAGCAGCTGATCGGCGAGCAGTCCGACATCGACAAGGAATATCTGGTGCGCGTGCGCTTCGGCGAGCACGTGATCGACG
TCGACCAGCATTTCCCGGCCGAATCGCTGGCCAAGCTGCGTCACGGCCTCGAACTCGACGGCGTGCCGCTGAAGCCCGCG
ATGGTGAGCTGGCAGAACAGCGAACAGCTGCGGTTCGTGCTGCGTGAAGGCAAGAAGCGGCAGATCCGCCGCATGTGCGA
GCTGGTCGGGCTCGAGGTGGTCGGCCTGAAACGCGTGCGCATGGGCCGCGTGATGCTCGGTGCGCTGCCGCAGGGCCAGT
GGCGCTACCTGGGGGCCGACGAGACGTTCTGA

Upstream 100 bases:

>100_bases
TGCTCGACGGCGCCGGCTTGTCCGACAACCGGCTCCAGATCCAGGTCGAAACCGAGCTGCTGCGCGCGATCGCAGCCTGA
GTGAAAGAAAGACAGCGTTT

Downstream 100 bases:

>100_bases
TCCTGACGGCGGCGCCGCGCGGCGCCGCCCACGATCCGGCGCGCGCGGCGGCCTGGCCGCGCCGCCAGCCATCTCCCGCG
CCTGCTCACGGCGCGCCGCC

Product: Pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MRTKLTIKHPRPASPSRAPVRSGSLVARKPTRPAAPPDGAPKPKSKPSAKGAAPRAAKPAGAGAVKPRAPREADRPSRSE
ARPARDGERPFRSEGRPARDGERPVRSGARPPRDGERPFRSEGRPARDGERPARNGARPPRDGERPFRSEGRPARDGERP
VRSGARPPRDGERPFRSEGRPARDGERPVRSGARPPRDGERPFRSEGRPARDGERPLRGTARPVRDGERPVRAGTRPPRD
GDRPSRVESRPARDTDRPARKPRVTTERPAPSRLKVAQPVKARPSFDSGDEAGMMRLSKRMSELGLCSRREADEWIEKGW
VLVDGERIDTLGTKVRPDQKIEIDDQAIAAQAAQVTILLHKPVGYVSGQAEDGYEPAVTLITRENHWSGDRSALRLSPLH
LRTLAPAGRLDIDSTGLLVLTQDGRIAKQLIGEQSDIDKEYLVRVRFGEHVIDVDQHFPAESLAKLRHGLELDGVPLKPA
MVSWQNSEQLRFVLREGKKRQIRRMCELVGLEVVGLKRVRMGRVMLGALPQGQWRYLGADETF

Sequences:

>Translated_543_residues
MRTKLTIKHPRPASPSRAPVRSGSLVARKPTRPAAPPDGAPKPKSKPSAKGAAPRAAKPAGAGAVKPRAPREADRPSRSE
ARPARDGERPFRSEGRPARDGERPVRSGARPPRDGERPFRSEGRPARDGERPARNGARPPRDGERPFRSEGRPARDGERP
VRSGARPPRDGERPFRSEGRPARDGERPVRSGARPPRDGERPFRSEGRPARDGERPLRGTARPVRDGERPVRAGTRPPRD
GDRPSRVESRPARDTDRPARKPRVTTERPAPSRLKVAQPVKARPSFDSGDEAGMMRLSKRMSELGLCSRREADEWIEKGW
VLVDGERIDTLGTKVRPDQKIEIDDQAIAAQAAQVTILLHKPVGYVSGQAEDGYEPAVTLITRENHWSGDRSALRLSPLH
LRTLAPAGRLDIDSTGLLVLTQDGRIAKQLIGEQSDIDKEYLVRVRFGEHVIDVDQHFPAESLAKLRHGLELDGVPLKPA
MVSWQNSEQLRFVLREGKKRQIRRMCELVGLEVVGLKRVRMGRVMLGALPQGQWRYLGADETF
>Mature_543_residues
MRTKLTIKHPRPASPSRAPVRSGSLVARKPTRPAAPPDGAPKPKSKPSAKGAAPRAAKPAGAGAVKPRAPREADRPSRSE
ARPARDGERPFRSEGRPARDGERPVRSGARPPRDGERPFRSEGRPARDGERPARNGARPPRDGERPFRSEGRPARDGERP
VRSGARPPRDGERPFRSEGRPARDGERPVRSGARPPRDGERPFRSEGRPARDGERPLRGTARPVRDGERPVRAGTRPPRD
GDRPSRVESRPARDTDRPARKPRVTTERPAPSRLKVAQPVKARPSFDSGDEAGMMRLSKRMSELGLCSRREADEWIEKGW
VLVDGERIDTLGTKVRPDQKIEIDDQAIAAQAAQVTILLHKPVGYVSGQAEDGYEPAVTLITRENHWSGDRSALRLSPLH
LRTLAPAGRLDIDSTGLLVLTQDGRIAKQLIGEQSDIDKEYLVRVRFGEHVIDVDQHFPAESLAKLRHGLELDGVPLKPA
MVSWQNSEQLRFVLREGKKRQIRRMCELVGLEVVGLKRVRMGRVMLGALPQGQWRYLGADETF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=251, Percent_Identity=35.8565737051793, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1787524, Length=254, Percent_Identity=32.6771653543307, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788510, Length=251, Percent_Identity=30.2788844621514, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 60232; Mature: 60232

Theoretical pI: Translated: 11.64; Mature: 11.64

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTKLTIKHPRPASPSRAPVRSGSLVARKPTRPAAPPDGAPKPKSKPSAKGAAPRAAKPA
CCCCEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GAGAVKPRAPREADRPSRSEARPARDGERPFRSEGRPARDGERPVRSGARPPRDGERPFR
CCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHCCCCCCCCCCHHHCCCCCCCCCCCCHH
SEGRPARDGERPARNGARPPRDGERPFRSEGRPARDGERPVRSGARPPRDGERPFRSEGR
HCCCCCCCCCCHHHCCCCCCCCCCCCHHHCCCCCCCCCCHHHCCCCCCCCCCCCHHHCCC
PARDGERPVRSGARPPRDGERPFRSEGRPARDGERPLRGTARPVRDGERPVRAGTRPPRD
CCCCCCCHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GDRPSRVESRPARDTDRPARKPRVTTERPAPSRLKVAQPVKARPSFDSGDEAGMMRLSKR
CCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MSELGLCSRREADEWIEKGWVLVDGERIDTLGTKVRPDQKIEIDDQAIAAQAAQVTILLH
HHHHCCHHCCHHHHHHHCCCEEECCCEEHHCCCCCCCCCCEECCHHHHHHHHEEEEEEEE
KPVGYVSGQAEDGYEPAVTLITRENHWSGDRSALRLSPLHLRTLAPAGRLDIDSTGLLVL
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCHHHHHCCCCCCCCCCCCCEEEE
TQDGRIAKQLIGEQSDIDKEYLVRVRFGEHVIDVDQHFPAESLAKLRHGLELDGVPLKPA
ECCCHHHHHHCCCCCCCCHHHEEEEECCCEEEECHHCCCHHHHHHHHHCCCCCCCCCCHH
MVSWQNSEQLRFVLREGKKRQIRRMCELVGLEVVGLKRVRMGRVMLGALPQGQWRYLGAD
HCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHCCCCCCEEECCCC
ETF
CCC
>Mature Secondary Structure
MRTKLTIKHPRPASPSRAPVRSGSLVARKPTRPAAPPDGAPKPKSKPSAKGAAPRAAKPA
CCCCEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GAGAVKPRAPREADRPSRSEARPARDGERPFRSEGRPARDGERPVRSGARPPRDGERPFR
CCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHCCCCCCCCCCHHHCCCCCCCCCCCCHH
SEGRPARDGERPARNGARPPRDGERPFRSEGRPARDGERPVRSGARPPRDGERPFRSEGR
HCCCCCCCCCCHHHCCCCCCCCCCCCHHHCCCCCCCCCCHHHCCCCCCCCCCCCHHHCCC
PARDGERPVRSGARPPRDGERPFRSEGRPARDGERPLRGTARPVRDGERPVRAGTRPPRD
CCCCCCCHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GDRPSRVESRPARDTDRPARKPRVTTERPAPSRLKVAQPVKARPSFDSGDEAGMMRLSKR
CCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MSELGLCSRREADEWIEKGWVLVDGERIDTLGTKVRPDQKIEIDDQAIAAQAAQVTILLH
HHHHCCHHCCHHHHHHHCCCEEECCCEEHHCCCCCCCCCCEECCHHHHHHHHEEEEEEEE
KPVGYVSGQAEDGYEPAVTLITRENHWSGDRSALRLSPLHLRTLAPAGRLDIDSTGLLVL
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCHHHHHCCCCCCCCCCCCCEEEE
TQDGRIAKQLIGEQSDIDKEYLVRVRFGEHVIDVDQHFPAESLAKLRHGLELDGVPLKPA
ECCCHHHHHHCCCCCCCCHHHEEEEECCCEEEECHHCCCHHHHHHHHHCCCCCCCCCCHH
MVSWQNSEQLRFVLREGKKRQIRRMCELVGLEVVGLKRVRMGRVMLGALPQGQWRYLGAD
HCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHCCCCCCEEECCCC
ETF
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]