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The map label for this gene is cls [H]

Identifier: 238026888

GI number: 238026888

Start: 1374475

End: 1375914

Strand: Reverse

Name: cls [H]

Synonym: bglu_1g12560

Alternate gene names: 238026888

Gene position: 1375914-1374475 (Counterclockwise)

Preceding gene: 238026889

Following gene: 238026886

Centisome position: 35.22

GC content: 70.28

Gene sequence:

>1440_bases
ATGCAACTCGACTGGCTCCATCTCGGCACCTTCGTCGCGCTCGCCCACATCCTCGGGACGATCGCGGCATGCCACGCGAT
CCTCAACACCCGCACCTCGCAGGGCGCGATCGCCTGGGCGGTCTCGCTGACCGCGATGCCGTACCTGACGCTGATCCCCT
ACCTGTTCCTCGGCCGCAGCAAGTTCTCCGGCTACGTGAACGCGCGCCGCCACGAGACGGCCGCGCTGCGCACGCGCTCG
CGCCCCTCGGCCTGGGACACGCACGGCAGCTCGCTCGGCGCCCCGGCCGATACGCTCGGGCTCGCGACGGTGCGCGCGCT
GACCCACCTCGGCGGCATGCCGTTCGTGGGCGGCAACGCGGTGCGGCTGCTGGTGAACGGCGAGGCGACGTTCGCGGCGA
TCCTCGCGGCGATCGAAGCGGCGCGTCACTACGTGATCGTGCAGTTCTTCATCGTGCGCGACGACACGCTCGGCGAAATG
CTGCGCGACGCGCTGCTCGCCTGCGCCGCGCGCGGCCTGCGCTGCTATCTGCTGTACGACAGCATCGGCAGCTTCGACCT
GCCGCACGCCTACGTGAACCGGCTGCGCGCGGGCGGCGTCGAGGTCCATCCGTTCGCCACCCACAAGCAGTTCGTCAACC
GCTTCCAGCTCAACTTCCGCAATCACCGCAAGATCGTGGTGGTGGACGGCGAATGCGCGTTCGTGGGCGGCCACAACGTG
GGCGTGGAGTATCTCGGCGCGAACCCGCGGCTCTCGCCGTGGCGCGACACGCACATCGAGCTGCGCGGGCCGGTGGTCGC
CAACATCCAGTACGTGTTCGCGGAGGACTGGCACTGGGCCACCCAGTCGCTGCCGGCCGCCGCGCCGACGCCGCCCGCGG
CGCCGGACCAGGACATGCATTGCCTGGTGGTGCCGATGGGGCCGGCCGACAAGCAGGAGACCGGCTCGCTGTTCTTCGTC
GAGGCGATCCACGCGGCGCGCGAGCGGGTCTGGATCACCACGCCCTACCTGGTGCCCGACGAGGCCGTGATCGCGGCGCT
CAAGCTCGCGGTGATGCGCGGCGTGGACGTGCGGATCCTGATCCCGATCCGGCGCGACCACTACGTGGTGTTCGAGGCCT
CGAAGCTCTACGCGCGCGACCTGATCGAGGCCGGCGTGCGGATCTTCCGCTACCGGCCCGGCTTCCTGCACCAGAAGGTG
GTGCTGATCGACGGCAAGGCCGCCGCGATCGGCAGCGCGAACCTCGACAACCGTTCGTTCCGCCTCAACTTCGAGATCAT
GGTGCTGACCGTGGACGCCGGCTTCGCGGCCGACGTCGACGCGATGCTCACGGCCGACTTCGCCTGCGCCGACGAGGTCG
ATCGCGGCGAGCTGGCGCGCGCGGGCGCCTGGCGGCGCATGCTGATACACGTCGCGCGGCTGTTCGCGCCGATCCTCTGA

Upstream 100 bases:

>100_bases
GATGGCCCGCAACTCGCGCCGGACGGCGCGGTTCGATAAGATGCAGGACCGGCTGCCGCATCCCGCGGCGCGCCGCGAAC
CATCCGGACCTCCACGCTCG

Downstream 100 bases:

>100_bases
GCCGCCCCGGCCCCGGCCCCGGCACCGCGCCGCCGGGCAGCTCACAGCAGCTTTTCGATGTCCTCGGCGATCTCGTCGGG
CTTGGTCTGCGGCGCGTAGC

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MQLDWLHLGTFVALAHILGTIAACHAILNTRTSQGAIAWAVSLTAMPYLTLIPYLFLGRSKFSGYVNARRHETAALRTRS
RPSAWDTHGSSLGAPADTLGLATVRALTHLGGMPFVGGNAVRLLVNGEATFAAILAAIEAARHYVIVQFFIVRDDTLGEM
LRDALLACAARGLRCYLLYDSIGSFDLPHAYVNRLRAGGVEVHPFATHKQFVNRFQLNFRNHRKIVVVDGECAFVGGHNV
GVEYLGANPRLSPWRDTHIELRGPVVANIQYVFAEDWHWATQSLPAAAPTPPAAPDQDMHCLVVPMGPADKQETGSLFFV
EAIHAARERVWITTPYLVPDEAVIAALKLAVMRGVDVRILIPIRRDHYVVFEASKLYARDLIEAGVRIFRYRPGFLHQKV
VLIDGKAAAIGSANLDNRSFRLNFEIMVLTVDAGFAADVDAMLTADFACADEVDRGELARAGAWRRMLIHVARLFAPIL

Sequences:

>Translated_479_residues
MQLDWLHLGTFVALAHILGTIAACHAILNTRTSQGAIAWAVSLTAMPYLTLIPYLFLGRSKFSGYVNARRHETAALRTRS
RPSAWDTHGSSLGAPADTLGLATVRALTHLGGMPFVGGNAVRLLVNGEATFAAILAAIEAARHYVIVQFFIVRDDTLGEM
LRDALLACAARGLRCYLLYDSIGSFDLPHAYVNRLRAGGVEVHPFATHKQFVNRFQLNFRNHRKIVVVDGECAFVGGHNV
GVEYLGANPRLSPWRDTHIELRGPVVANIQYVFAEDWHWATQSLPAAAPTPPAAPDQDMHCLVVPMGPADKQETGSLFFV
EAIHAARERVWITTPYLVPDEAVIAALKLAVMRGVDVRILIPIRRDHYVVFEASKLYARDLIEAGVRIFRYRPGFLHQKV
VLIDGKAAAIGSANLDNRSFRLNFEIMVLTVDAGFAADVDAMLTADFACADEVDRGELARAGAWRRMLIHVARLFAPIL
>Mature_479_residues
MQLDWLHLGTFVALAHILGTIAACHAILNTRTSQGAIAWAVSLTAMPYLTLIPYLFLGRSKFSGYVNARRHETAALRTRS
RPSAWDTHGSSLGAPADTLGLATVRALTHLGGMPFVGGNAVRLLVNGEATFAAILAAIEAARHYVIVQFFIVRDDTLGEM
LRDALLACAARGLRCYLLYDSIGSFDLPHAYVNRLRAGGVEVHPFATHKQFVNRFQLNFRNHRKIVVVDGECAFVGGHNV
GVEYLGANPRLSPWRDTHIELRGPVVANIQYVFAEDWHWATQSLPAAAPTPPAAPDQDMHCLVVPMGPADKQETGSLFFV
EAIHAARERVWITTPYLVPDEAVIAALKLAVMRGVDVRILIPIRRDHYVVFEASKLYARDLIEAGVRIFRYRPGFLHQKV
VLIDGKAAAIGSANLDNRSFRLNFEIMVLTVDAGFAADVDAMLTADFACADEVDRGELARAGAWRRMLIHVARLFAPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=475, Percent_Identity=27.3684210526316, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1787007, Length=349, Percent_Identity=31.5186246418338, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI87081826, Length=385, Percent_Identity=27.7922077922078, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 52700; Mature: 52700

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLDWLHLGTFVALAHILGTIAACHAILNTRTSQGAIAWAVSLTAMPYLTLIPYLFLGRS
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCC
KFSGYVNARRHETAALRTRSRPSAWDTHGSSLGAPADTLGLATVRALTHLGGMPFVGGNA
HHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCE
VRLLVNGEATFAAILAAIEAARHYVIVQFFIVRDDTLGEMLRDALLACAARGLRCYLLYD
EEEEECCCHHHHHHHHHHHHHCCEEEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEE
SIGSFDLPHAYVNRLRAGGVEVHPFATHKQFVNRFQLNFRNHRKIVVVDGECAFVGGHNV
CCCCCCCCHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCC
GVEYLGANPRLSPWRDTHIELRGPVVANIQYVFAEDWHWATQSLPAAAPTPPAAPDQDMH
CEEEECCCCCCCCCCCCCEEEECCEEEEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCE
CLVVPMGPADKQETGSLFFVEAIHAARERVWITTPYLVPDEAVIAALKLAVMRGVDVRIL
EEEEECCCCCCCCCCCEEEEHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
IPIRRDHYVVFEASKLYARDLIEAGVRIFRYRPGFLHQKVVLIDGKAAAIGSANLDNRSF
EEECCCEEEEEECHHHHHHHHHHHCCEEEEECCCCCCCEEEEEECCEEEECCCCCCCCEE
RLNFEIMVLTVDAGFAADVDAMLTADFACADEVDRGELARAGAWRRMLIHVARLFAPIL
EEEEEEEEEEECCCCCCCCHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQLDWLHLGTFVALAHILGTIAACHAILNTRTSQGAIAWAVSLTAMPYLTLIPYLFLGRS
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCC
KFSGYVNARRHETAALRTRSRPSAWDTHGSSLGAPADTLGLATVRALTHLGGMPFVGGNA
HHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCE
VRLLVNGEATFAAILAAIEAARHYVIVQFFIVRDDTLGEMLRDALLACAARGLRCYLLYD
EEEEECCCHHHHHHHHHHHHHCCEEEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEE
SIGSFDLPHAYVNRLRAGGVEVHPFATHKQFVNRFQLNFRNHRKIVVVDGECAFVGGHNV
CCCCCCCCHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCC
GVEYLGANPRLSPWRDTHIELRGPVVANIQYVFAEDWHWATQSLPAAAPTPPAAPDQDMH
CEEEECCCCCCCCCCCCCEEEECCEEEEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCE
CLVVPMGPADKQETGSLFFVEAIHAARERVWITTPYLVPDEAVIAALKLAVMRGVDVRIL
EEEEECCCCCCCCCCCEEEEHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
IPIRRDHYVVFEASKLYARDLIEAGVRIFRYRPGFLHQKVVLIDGKAAAIGSANLDNRSF
EEECCCEEEEEECHHHHHHHHHHHCCEEEEECCCCCCCEEEEEECCEEEECCCCCCCCEE
RLNFEIMVLTVDAGFAADVDAMLTADFACADEVDRGELARAGAWRRMLIHVARLFAPIL
EEEEEEEEEEECCCCCCCCHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA