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The map label for this gene is rlmE [H]

Identifier: 238026783

GI number: 238026783

Start: 1251751

End: 1252413

Strand: Direct

Name: rlmE [H]

Synonym: bglu_1g11410

Alternate gene names: 238026783

Gene position: 1251751-1252413 (Clockwise)

Preceding gene: 238026781

Following gene: 238026784

Centisome position: 32.04

GC content: 61.99

Gene sequence:

>663_bases
ATGGCAAAGAATCGTTTCAATCAGTCGTGGCTGCACGACCACATCAACGACCCCTACGTCAAAATGGCGCAGCGGGAAGG
CTATCGCGCACGCGCGGCATACAAGCTGAAAGAAATCGACGAACAGGACAAGCTGATCCGGCCCGGCCAGGTGATCGTCG
ATCTCGGCGCCGCGCCCGGCAGCTGGAGCCAGTACGCGCGCAACAAGCTCGCGCTCGGCAAAAACCGCGACGCGCAGCGC
GAGGGCGGCATCGACGGCATGATCGTCGCGCTCGACATGCTGCCGATGGAGCCGGTGGCCGACGTCCACTTCATCCAGGG
CGACTTCCGCGAGGACGCGGTGCTGCACCAGCTGGAGGCGCTGCTCGACGGCCGCGTGGTCGACCTTGTAATTTCCGACA
TGGCACCCAACCTGTCTGGCGTGGCGTCGGCGGATGCGGCACGGATCGAGCATGTGTGCGACCTGGCGCTCGAATTCTCG
CAGAACCATCTGAAGCCGGATGGTGCCCTTTTAGTGAAATGCTTTCACGGCAGCGGCTACAGCCAGATTGTCGAAAAGTT
CAAGCATCAGTTCCGCACGGTCGCGCCGCGCAAGCCGAAGGCGTCGCGAGACAAGTCGTCGGAGACGTTTATTTTGGGTC
GGCATCTGAAACATCCGCGCTGA

Upstream 100 bases:

>100_bases
GCCAAAAGCCCGGGCTGACGAAACCATTGCGCGATCGGCGCGGCAATCGCCCGTTTTCGCGGCCTGGTTTGTGGCCTTTT
GGCTGAATTTTTTCCTCTGC

Downstream 100 bases:

>100_bases
AGCGGATGCACGGGCCACCGCAGGCCGGCCGCAGCCTTGCCGGCCGTAGGGTTTGACTATCGTGGCGTTTGGTGAACCTT
ATGGCGACGGGCTTCGGACT

Product: Ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAKNRFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPGSWSQYARNKLALGKNRDAQR
EGGIDGMIVALDMLPMEPVADVHFIQGDFREDAVLHQLEALLDGRVVDLVISDMAPNLSGVASADAARIEHVCDLALEFS
QNHLKPDGALLVKCFHGSGYSQIVEKFKHQFRTVAPRKPKASRDKSSETFILGRHLKHPR

Sequences:

>Translated_220_residues
MAKNRFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPGSWSQYARNKLALGKNRDAQR
EGGIDGMIVALDMLPMEPVADVHFIQGDFREDAVLHQLEALLDGRVVDLVISDMAPNLSGVASADAARIEHVCDLALEFS
QNHLKPDGALLVKCFHGSGYSQIVEKFKHQFRTVAPRKPKASRDKSSETFILGRHLKHPR
>Mature_219_residues
AKNRFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPGSWSQYARNKLALGKNRDAQRE
GGIDGMIVALDMLPMEPVADVHFIQGDFREDAVLHQLEALLDGRVVDLVISDMAPNLSGVASADAARIEHVCDLALEFSQ
NHLKPDGALLVKCFHGSGYSQIVEKFKHQFRTVAPRKPKASRDKSSETFILGRHLKHPR

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=215, Percent_Identity=34.4186046511628, Blast_Score=135, Evalue=2e-32,
Organism=Homo sapiens, GI29029591, Length=203, Percent_Identity=31.5270935960591, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI29029589, Length=203, Percent_Identity=31.5270935960591, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI7110661, Length=203, Percent_Identity=31.5270935960591, Blast_Score=104, Evalue=5e-23,
Organism=Homo sapiens, GI194097365, Length=203, Percent_Identity=28.5714285714286, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1789569, Length=203, Percent_Identity=42.3645320197044, Blast_Score=179, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17553474, Length=225, Percent_Identity=32, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17554650, Length=203, Percent_Identity=32.512315270936, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71987550, Length=203, Percent_Identity=29.064039408867, Blast_Score=94, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71987561, Length=203, Percent_Identity=29.064039408867, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17553860, Length=203, Percent_Identity=29.064039408867, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6319535, Length=198, Percent_Identity=31.8181818181818, Blast_Score=102, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=205, Percent_Identity=29.7560975609756, Blast_Score=99, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321302, Length=102, Percent_Identity=39.2156862745098, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356387, Length=217, Percent_Identity=32.7188940092166, Blast_Score=126, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24648639, Length=203, Percent_Identity=30.0492610837438, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24647580, Length=205, Percent_Identity=30.2439024390244, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI18859957, Length=198, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 24688; Mature: 24556

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNRFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPG
CCCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SWSQYARNKLALGKNRDAQREGGIDGMIVALDMLPMEPVADVHFIQGDFREDAVLHQLEA
CHHHHHHHHHCCCCCCCCHHHCCCCEEEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHH
LLDGRVVDLVISDMAPNLSGVASADAARIEHVCDLALEFSQNHLKPDGALLVKCFHGSGY
HHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCH
SQIVEKFKHQFRTVAPRKPKASRDKSSETFILGRHLKHPR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
AKNRFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPG
CCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SWSQYARNKLALGKNRDAQREGGIDGMIVALDMLPMEPVADVHFIQGDFREDAVLHQLEA
CHHHHHHHHHCCCCCCCCHHHCCCCEEEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHH
LLDGRVVDLVISDMAPNLSGVASADAARIEHVCDLALEFSQNHLKPDGALLVKCFHGSGY
HHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCH
SQIVEKFKHQFRTVAPRKPKASRDKSSETFILGRHLKHPR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA