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The map label for this gene is mltD [H]

Identifier: 238026775

GI number: 238026775

Start: 1241302

End: 1242909

Strand: Direct

Name: mltD [H]

Synonym: bglu_1g11330

Alternate gene names: 238026775

Gene position: 1241302-1242909 (Clockwise)

Preceding gene: 238026774

Following gene: 238026776

Centisome position: 31.78

GC content: 67.35

Gene sequence:

>1608_bases
ATGCGATTCCTGATCAGTGCGCTATTGGTTTTGCTGCTCGCCGCTTGCGCGAGCCCCGGACCGACCGCCCCATCCGCCGC
TTCCGACCCGCAGGCCGCTGCCGACTTCATTCGCAAGACCTCGACCGCCAAAGACACCGTCGACGTCGACAAGCAGTCGG
TCGGCGATCTGACCACCAAGGATTCCGATCTCTGGGCGCGCATCCGCCGCGGCTTTCAGATCCCCGATCTGCAGAGCGAC
CTGGTCGACATGCAGACCACCTGGTATACCGAGCGTCCCGACTACGTGCAGCGCATGACGGAGCGCTCGCAAAAGTATCT
CTACCATATCGTCGAGGAGCTCGAGGCGCGCAACATGCCGACCGAGCTCGCGCTGCTGCCGTTCATCGAATCGGCCTACA
ACCCGCAGGCGCTGTCGGTCGCGAAGGCCGCCGGCATGTGGCAGTTCATTCCCGGCACCGGCCGCACCTACAACCTGAAG
CAGAACATGTGGCAGGACGAGCGCCGCGACGTGCTGGCCTCGACCAGCGCCGCGCTCGACTACCTGTCGCGCCTGCATGA
CATGTTCGGCGACTGGTATCTGGCGCTGGCCGCCTACAACTGGGGCGAGGGCAACGTGCAGCGCGCGATCGCGCGCAACC
AGGCGGCCGGCCTGCCGACCGACTACCAGAGCCTGCGCATGCCGAACGAGACGCGCAACTACGTGCCGAAGCTGCAGGCG
GTGAAGAACATCATCGCCCATCCGCAACAGTACGGCCTGACGCTGCCCGACATTCCGAACCATCCGTATTTCGTGACGGT
CACGACCTCGCACGACATCGACGTGAAGATGGCGGCGCAACTGGCCGGCCTCTCGCTCGAGGAGTTCAAGGCGCTCAACC
CGTCTTTCTCGAAGCCGGTGATACTCGGCGCGACCCAGCCGCAGATCCTGCTGCCGTTCGACAATGCCTCGCAGTTCGAG
AAGGGCCTGAAGTCGTATAACGGCCAGCTCTCCAGCTGGACCACCTATACGGTCACCGAGCGTGCGCGGCCGGCGGCGAT
CGCCGAGAAGATCGGGGTCGACGCCGACACGCTGATGCAGGTGAACAAGATTCCGGCCGGAATGCGGCTGAAGCCGGGCT
CGACCATCGTCGTGCCGCGCAGCGACGACGACGACGAGGACATCAGCGCCGACGTGGCCGAGAACGCCGTGCTCGCGATG
GAGCCCGACGTGCCCGACACGCGCAAGATGCTGATCCGCGTGCGCCGCAAGCAGTCGATGGCCGCGCTGGCGAGCCGCTA
CGGCGTGTCGGTCGGCCAACTGAAGGGTTGGAACCGCACCCGCCGCGACCTGGTGATGCCGGGCCAGACCATCGTCCTGC
ACGTGCCGGCGGGCCGTGCCGTGCCGGCCGAACCCGGCCCCGAGCGGATCGCCACGTCGGTGGGCGGCGCACGCATCGAA
CGCGCGAGCCTGCACACGGGCGGTACGGCCGCCGCGCGCGGGCGGCACGCGGCCCCGGCGAAGGCGGCGGCCCGATCGAC
CGCGAAGTCGACCGCCAGGTCGACGGCCGCCGCGAAGAGCGCGAAGCCGGCCGTCAAGCCCGCGGCGCATAAGGCGAAGA
AGAAATAA

Upstream 100 bases:

>100_bases
ATACCGGATTTATCGGCATTTTTCGGCTTTTTATTGACGCGATGCCGGCGCCTCCCTTACTATCGCCTGCAATTTCCAGC
CGTTGGAAGCCGAGATTTAC

Downstream 100 bases:

>100_bases
CGCGATTCGGCCGCCGGCGGCGTGGGGCGTCGTGCCCGATGCGCCGCGCCTGGCTCGTGATTCGCGCGCACGCCGTCACC
GAGGTGACGGCGTTTTCGTT

Product: putative exported transglycosylase protein

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MRFLISALLVLLLAACASPGPTAPSAASDPQAAADFIRKTSTAKDTVDVDKQSVGDLTTKDSDLWARIRRGFQIPDLQSD
LVDMQTTWYTERPDYVQRMTERSQKYLYHIVEELEARNMPTELALLPFIESAYNPQALSVAKAAGMWQFIPGTGRTYNLK
QNMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQA
VKNIIAHPQQYGLTLPDIPNHPYFVTVTTSHDIDVKMAAQLAGLSLEEFKALNPSFSKPVILGATQPQILLPFDNASQFE
KGLKSYNGQLSSWTTYTVTERARPAAIAEKIGVDADTLMQVNKIPAGMRLKPGSTIVVPRSDDDDEDISADVAENAVLAM
EPDVPDTRKMLIRVRRKQSMAALASRYGVSVGQLKGWNRTRRDLVMPGQTIVLHVPAGRAVPAEPGPERIATSVGGARIE
RASLHTGGTAAARGRHAAPAKAAARSTAKSTARSTAAAKSAKPAVKPAAHKAKKK

Sequences:

>Translated_535_residues
MRFLISALLVLLLAACASPGPTAPSAASDPQAAADFIRKTSTAKDTVDVDKQSVGDLTTKDSDLWARIRRGFQIPDLQSD
LVDMQTTWYTERPDYVQRMTERSQKYLYHIVEELEARNMPTELALLPFIESAYNPQALSVAKAAGMWQFIPGTGRTYNLK
QNMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQA
VKNIIAHPQQYGLTLPDIPNHPYFVTVTTSHDIDVKMAAQLAGLSLEEFKALNPSFSKPVILGATQPQILLPFDNASQFE
KGLKSYNGQLSSWTTYTVTERARPAAIAEKIGVDADTLMQVNKIPAGMRLKPGSTIVVPRSDDDDEDISADVAENAVLAM
EPDVPDTRKMLIRVRRKQSMAALASRYGVSVGQLKGWNRTRRDLVMPGQTIVLHVPAGRAVPAEPGPERIATSVGGARIE
RASLHTGGTAAARGRHAAPAKAAARSTAKSTARSTAAAKSAKPAVKPAAHKAKKK
>Mature_535_residues
MRFLISALLVLLLAACASPGPTAPSAASDPQAAADFIRKTSTAKDTVDVDKQSVGDLTTKDSDLWARIRRGFQIPDLQSD
LVDMQTTWYTERPDYVQRMTERSQKYLYHIVEELEARNMPTELALLPFIESAYNPQALSVAKAAGMWQFIPGTGRTYNLK
QNMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQA
VKNIIAHPQQYGLTLPDIPNHPYFVTVTTSHDIDVKMAAQLAGLSLEEFKALNPSFSKPVILGATQPQILLPFDNASQFE
KGLKSYNGQLSSWTTYTVTERARPAAIAEKIGVDADTLMQVNKIPAGMRLKPGSTIVVPRSDDDDEDISADVAENAVLAM
EPDVPDTRKMLIRVRRKQSMAALASRYGVSVGQLKGWNRTRRDLVMPGQTIVLHVPAGRAVPAEPGPERIATSVGGARIE
RASLHTGGTAAARGRHAAPAKAAARSTAKSTARSTAAAKSAKPAVKPAAHKAKKK

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=462, Percent_Identity=35.2813852813853, Blast_Score=244, Evalue=1e-65,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 58471; Mature: 58471

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLISALLVLLLAACASPGPTAPSAASDPQAAADFIRKTSTAKDTVDVDKQSVGDLTTK
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCHHHHCCCCCC
DSDLWARIRRGFQIPDLQSDLVDMQTTWYTERPDYVQRMTERSQKYLYHIVEELEARNMP
CHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TELALLPFIESAYNPQALSVAKAAGMWQFIPGTGRTYNLKQNMWQDERRDVLASTSAALD
HHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHH
YLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQA
HHHHHHHHHHHHEEHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHH
VKNIIAHPQQYGLTLPDIPNHPYFVTVTTSHDIDVKMAAQLAGLSLEEFKALNPSFSKPV
HHHHHCCHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHCCCCCCCCE
ILGATQPQILLPFDNASQFEKGLKSYNGQLSSWTTYTVTERARPAAIAEKIGVDADTLMQ
EEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHH
VNKIPAGMRLKPGSTIVVPRSDDDDEDISADVAENAVLAMEPDVPDTRKMLIRVRRKQSM
HHCCCCCCEECCCCEEEEECCCCCCCCCHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHH
AALASRYGVSVGQLKGWNRTRRDLVMPGQTIVLHVPAGRAVPAEPGPERIATSVGGARIE
HHHHHHHCCCHHHCCCCCHHHHCEECCCCEEEEECCCCCCCCCCCCHHHHHHHCCCHHEE
RASLHTGGTAAARGRHAAPAKAAARSTAKSTARSTAAAKSAKPAVKPAAHKAKKK
HHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MRFLISALLVLLLAACASPGPTAPSAASDPQAAADFIRKTSTAKDTVDVDKQSVGDLTTK
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCHHHHCCCCCC
DSDLWARIRRGFQIPDLQSDLVDMQTTWYTERPDYVQRMTERSQKYLYHIVEELEARNMP
CHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TELALLPFIESAYNPQALSVAKAAGMWQFIPGTGRTYNLKQNMWQDERRDVLASTSAALD
HHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHH
YLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQA
HHHHHHHHHHHHEEHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHH
VKNIIAHPQQYGLTLPDIPNHPYFVTVTTSHDIDVKMAAQLAGLSLEEFKALNPSFSKPV
HHHHHCCHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHCCCCCCCCE
ILGATQPQILLPFDNASQFEKGLKSYNGQLSSWTTYTVTERARPAAIAEKIGVDADTLMQ
EEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHH
VNKIPAGMRLKPGSTIVVPRSDDDDEDISADVAENAVLAMEPDVPDTRKMLIRVRRKQSM
HHCCCCCCEECCCCEEEEECCCCCCCCCHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHH
AALASRYGVSVGQLKGWNRTRRDLVMPGQTIVLHVPAGRAVPAEPGPERIATSVGGARIE
HHHHHHHCCCHHHCCCCCHHHHCEECCCCEEEEECCCCCCCCCCCCHHHHHHHCCCHHEE
RASLHTGGTAAARGRHAAPAKAAARSTAKSTARSTAAAKSAKPAVKPAAHKAKKK
HHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]