Click here to switch to the map view.

The map label for this gene is gloB [H]

Identifier: 238026774

GI number: 238026774

Start: 1240227

End: 1241033

Strand: Direct

Name: gloB [H]

Synonym: bglu_1g11320

Alternate gene names: 238026774

Gene position: 1240227-1241033 (Clockwise)

Preceding gene: 238026770

Following gene: 238026775

Centisome position: 31.75

GC content: 71.75

Gene sequence:

>807_bases
ATGAACGAGCTGGAATACGTGCCGGTGCCGGCGTTTGAGGACAACTATATCTGGCTCGTGTCGGATGGCAGCGACGCGGT
CGCCGTCGATCCGGGCGACGCCGCCCCGGTGCGCCGATATCTCGACGCGCGAGGCTGGCGGCTAGCCGCTATTCTACTCA
CGCACCATCACGCCGACCATGTCGGCGGCGTCTCGGCGCTCGTCGGCGAGCGCGAGCAGATCATGCCCGTGCCGGTTTAC
GGCCCGGCCGGCGAGGCGATCGCCCAGGTCACGCAGCCGCTCGCGGGCGGCGCGCGCGTGCGCATCGGCAAGCCGGCACT
CGAGTTCGAGGTGCTGGACGTGCCGGGGCACACGCGCGGCCACATCGCTTATTTCCAGGCGGCCGGCGCCGGCCACCCGG
CGCCGCATCTGTTCTGCGGCGACACGCTGTTCTCGTGCGGCTGCGGGCGCCTGTTCGAAGGCACGCCCGAGCAGATGCTG
AACTCGCTCGACGCGCTGGCTGCGCTGCCCGGCGACACGCAGGTGCATTGCGCCCACGAATACACGCTCTCGAACATCCG
CTTCGCGCTCGCCTGCGAGCCCGACAACGCGGCATTGGCCGCGTGGCGCGACCGCGCCGAGGCGTGTCGGGCGCGCGGCG
AGCCGACCCTGCCCACCACCATCGCGCACGAGCGCGCGGTCAACCCGTTCCTGCGCGCCGACAGCGCCGCGATCGCCGCG
ACGCTCGAGGCCGAACTGCACGAGGCGCCGGCGAGCCGGCTCGCCGCGTTCACGCTGCTGCGCGAGTGGAAGAACCGCTT
CCGCTGA

Upstream 100 bases:

>100_bases
GGGCCTGTTCCCACTCGAGCACGTAACGGCCGGGCGGCGAGGCGCTCCAGGCGGGCCAGTCTATAATCGGACGGTCTGAC
ATATCGATGAGTGCGCGCCC

Downstream 100 bases:

>100_bases
ACCGCCCGCATGGCCGCCCCCGTCGCGCTTCGCCGCTGATCGTGCCGCTGATCGCGCCGGCAGCCGGCGGCGGCGCGACG
AGGTGGCGCGGGGGCTTTCC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNELEYVPVPAFEDNYIWLVSDGSDAVAVDPGDAAPVRRYLDARGWRLAAILLTHHHADHVGGVSALVGEREQIMPVPVY
GPAGEAIAQVTQPLAGGARVRIGKPALEFEVLDVPGHTRGHIAYFQAAGAGHPAPHLFCGDTLFSCGCGRLFEGTPEQML
NSLDALAALPGDTQVHCAHEYTLSNIRFALACEPDNAALAAWRDRAEACRARGEPTLPTTIAHERAVNPFLRADSAAIAA
TLEAELHEAPASRLAAFTLLREWKNRFR

Sequences:

>Translated_268_residues
MNELEYVPVPAFEDNYIWLVSDGSDAVAVDPGDAAPVRRYLDARGWRLAAILLTHHHADHVGGVSALVGEREQIMPVPVY
GPAGEAIAQVTQPLAGGARVRIGKPALEFEVLDVPGHTRGHIAYFQAAGAGHPAPHLFCGDTLFSCGCGRLFEGTPEQML
NSLDALAALPGDTQVHCAHEYTLSNIRFALACEPDNAALAAWRDRAEACRARGEPTLPTTIAHERAVNPFLRADSAAIAA
TLEAELHEAPASRLAAFTLLREWKNRFR
>Mature_268_residues
MNELEYVPVPAFEDNYIWLVSDGSDAVAVDPGDAAPVRRYLDARGWRLAAILLTHHHADHVGGVSALVGEREQIMPVPVY
GPAGEAIAQVTQPLAGGARVRIGKPALEFEVLDVPGHTRGHIAYFQAAGAGHPAPHLFCGDTLFSCGCGRLFEGTPEQML
NSLDALAALPGDTQVHCAHEYTLSNIRFALACEPDNAALAAWRDRAEACRARGEPTLPTTIAHERAVNPFLRADSAAIAA
TLEAELHEAPASRLAAFTLLREWKNRFR

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=263, Percent_Identity=35.7414448669202, Blast_Score=161, Evalue=6e-40,
Organism=Homo sapiens, GI94538322, Length=263, Percent_Identity=35.7414448669202, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI116642887, Length=245, Percent_Identity=38.3673469387755, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI21703352, Length=245, Percent_Identity=38.3673469387755, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI14150041, Length=226, Percent_Identity=40.2654867256637, Blast_Score=140, Evalue=9e-34,
Organism=Homo sapiens, GI46361987, Length=190, Percent_Identity=40, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1786406, Length=265, Percent_Identity=46.0377358490566, Blast_Score=204, Evalue=4e-54,
Organism=Escherichia coli, GI1787158, Length=193, Percent_Identity=34.1968911917098, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=262, Percent_Identity=34.7328244274809, Blast_Score=130, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6320478, Length=265, Percent_Identity=28.3018867924528, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324614, Length=265, Percent_Identity=28.6792452830189, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356335, Length=238, Percent_Identity=39.0756302521008, Blast_Score=163, Evalue=9e-41,
Organism=Drosophila melanogaster, GI24667711, Length=238, Percent_Identity=39.0756302521008, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24667703, Length=238, Percent_Identity=39.0756302521008, Blast_Score=162, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28866; Mature: 28866

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELEYVPVPAFEDNYIWLVSDGSDAVAVDPGDAAPVRRYLDARGWRLAAILLTHHHADH
CCCCCEEECCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCCHH
VGGVSALVGEREQIMPVPVYGPAGEAIAQVTQPLAGGARVRIGKPALEFEVLDVPGHTRG
HCCHHHHHCCCHHEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCCCC
HIAYFQAAGAGHPAPHLFCGDTLFSCGCGRLFEGTPEQMLNSLDALAALPGDTQVHCAHE
CEEEEEECCCCCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
YTLSNIRFALACEPDNAALAAWRDRAEACRARGEPTLPTTIAHERAVNPFLRADSAAIAA
EECCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHCCCHHHHH
TLEAELHEAPASRLAAFTLLREWKNRFR
HHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNELEYVPVPAFEDNYIWLVSDGSDAVAVDPGDAAPVRRYLDARGWRLAAILLTHHHADH
CCCCCEEECCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCCHH
VGGVSALVGEREQIMPVPVYGPAGEAIAQVTQPLAGGARVRIGKPALEFEVLDVPGHTRG
HCCHHHHHCCCHHEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCCCC
HIAYFQAAGAGHPAPHLFCGDTLFSCGCGRLFEGTPEQMLNSLDALAALPGDTQVHCAHE
CEEEEEECCCCCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
YTLSNIRFALACEPDNAALAAWRDRAEACRARGEPTLPTTIAHERAVNPFLRADSAAIAA
EECCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHCCCHHHHH
TLEAELHEAPASRLAAFTLLREWKNRFR
HHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA