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The map label for this gene is lepA [H]

Identifier: 238026615

GI number: 238026615

Start: 1036865

End: 1038658

Strand: Direct

Name: lepA [H]

Synonym: bglu_1g09620

Alternate gene names: 238026615

Gene position: 1036865-1038658 (Clockwise)

Preceding gene: 238026614

Following gene: 238026616

Centisome position: 26.54

GC content: 63.77

Gene sequence:

>1794_bases
ATGGATCATATTCGCAATTTCTCGATCATCGCCCACATCGACCATGGCAAGTCGACGCTCGCGGATCGCATCATCCAGGT
TTGCGGCGGCCTGACCGACCGTGAAATGGAAGCCCAGGTGCTCGACTCGATGGACCTCGAACGCGAGCGCGGCATCACGA
TCAAGGCCCAGACGGCGGCGCTCACGTACCGCGCCCGCGACGGCAAGGTCTATAACCTCAACCTGATCGACACGCCGGGG
CACGTCGACTTCTCCTACGAAGTCAGCCGCTCGCTGTCGGCCTGCGAGGGCGCGCTGCTGGTGGTGGACGCGAGCCAGGG
CGTCGAGGCCCAGACGGTCGCGAACTGCTACACGGCGATCGAGCTCGGCGTCGAGGTGGTGCCGGTGCTGAACAAGATCG
ACCTGCCTGCGGCGAACCCCGACAACGCGATCACCGAGATCGAGGACGTGATCGGCATCGACGCGACCGACGCGACGCGC
TGCAGCGCGAAGACCGGCCTCGGCGTCGAGGACGTGCTCGAGTCGCTGATCGCCAAGGTGCCGCCGCCGAAGGGCGATCC
GGACGCGCCGCTGCAGGCGCTCATCATCGATTCGTGGTTCGACAACTACGTCGGCGTGGTGATGCTGGTGCGCATCGTCA
ACGGTACGCTGCGCCCGAAGGACAAGATCAAGCTGATGGCCACCGGCGCGCAGTTCCCGGTCGAGCACATCGGCGTGTTC
ACGCCGAAGTCGCGCAACCTGGAATCGCTCTCGGCGGGGCAGGTCGGCTTCATCATCGCCGGCATCAAGGAACTGACGGC
CGCCAAGGTGGGCGACACCGTCACGCACGTGACGAAGGCCGCCGCCGAGCCGCTGCCGGGCTTCAAGGAAGTGAAGCCGC
AGGTGTTCGCGGGCCTCTATCCGGTCGAGGCGAACCAGTACGACGCGCTGCGCGAATCGCTCGAGAAGCTCAAGCTCAAC
GACGCCTCGCTGCAGTACGAGCCGGAAGTCTCGCAGGCGCTCGGCTTCGGCTTCCGCTGCGGCTTCCTGGGCCTGCTGCA
CATGGAGATCGTGCAGGAGCGCCTCGAACGCGAGTTCGACATGGACCTCATCACCACCGCGCCGACGGTGGTCTACGAGG
TGGTGCAGAGCGACGGCACGACCATCATGGTCGAGAATCCGGCGAAGATGCCCGAGCCGGCCCGCATCGAGGAGATTCGC
GAGCCGATCGTGACCGTGAACCTCTACATGCCGCAGGACTACGTCGGCTCGGTCATCACGCTCTGCACGCAAAAGCGCGG
TTCGCAGATCAACATGCAGTACCACGGCCGCCAGGTGCAACTGACCTACGAGATCCCGATGGCCGAAATCGTGCTCGACT
TTTTCGACCGGCTGAAGTCGGTGTCGCGCGGCTATGCGTCGATGGATTACGAATTCAAGGAGTATCGCGCCTCGGACGTG
GTCAAGGTCGACATGCTGATCAACGGCGACAAGGTCGACGCGCTGTCGGTCATCGTTCACCGTTCGCAGTCGCAATACCG
CGGCCGCGAGGTGGCGGCGAAGATGCGTGAGATCATCCCGCGCCAGATGTACGACGTCGCGATCCAGGCGGCGATCGGCG
CGCACATCATCGCGCGCGAGAATATCAAGGCGCTGCGCAAGAACGTGCTGGCCAAGTGCTACGGCGGCGACATCACGCGC
AAGAAGAAGCTGCTGGAAAAGCAGAAGGAAGGCAAAAAGCGCATGAAGCAGGTGGGTTCGGTCGAGATCCCGCAGGAAGC
GTTCCTCGCGATCTTGCGTGTCGAAGACAAATAA

Upstream 100 bases:

>100_bases
CCGGACAGCCCTTTTCGGCTAAAATAGGCCGTTTTTTCACCGACTTACAAGGCGTGCTCCGCAGTCGTCGAGCGCGCCTT
TTTCGCTTGATCGGCACTGA

Downstream 100 bases:

>100_bases
CAGGACTGATCCTTTTATGAATTTCGCATTGATTCTTTTTGTGCTCGTCGTCTTGACGGGTATCGCGTGGGTGCTGGACA
AGCTGGTGTTCCTGCCGCGC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MDHIRNFSIIAHIDHGKSTLADRIIQVCGGLTDREMEAQVLDSMDLERERGITIKAQTAALTYRARDGKVYNLNLIDTPG
HVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVEVVPVLNKIDLPAANPDNAITEIEDVIGIDATDATR
CSAKTGLGVEDVLESLIAKVPPPKGDPDAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIKLMATGAQFPVEHIGVF
TPKSRNLESLSAGQVGFIIAGIKELTAAKVGDTVTHVTKAAAEPLPGFKEVKPQVFAGLYPVEANQYDALRESLEKLKLN
DASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVVQSDGTTIMVENPAKMPEPARIEEIR
EPIVTVNLYMPQDYVGSVITLCTQKRGSQINMQYHGRQVQLTYEIPMAEIVLDFFDRLKSVSRGYASMDYEFKEYRASDV
VKVDMLINGDKVDALSVIVHRSQSQYRGREVAAKMREIIPRQMYDVAIQAAIGAHIIARENIKALRKNVLAKCYGGDITR
KKKLLEKQKEGKKRMKQVGSVEIPQEAFLAILRVEDK

Sequences:

>Translated_597_residues
MDHIRNFSIIAHIDHGKSTLADRIIQVCGGLTDREMEAQVLDSMDLERERGITIKAQTAALTYRARDGKVYNLNLIDTPG
HVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVEVVPVLNKIDLPAANPDNAITEIEDVIGIDATDATR
CSAKTGLGVEDVLESLIAKVPPPKGDPDAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIKLMATGAQFPVEHIGVF
TPKSRNLESLSAGQVGFIIAGIKELTAAKVGDTVTHVTKAAAEPLPGFKEVKPQVFAGLYPVEANQYDALRESLEKLKLN
DASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVVQSDGTTIMVENPAKMPEPARIEEIR
EPIVTVNLYMPQDYVGSVITLCTQKRGSQINMQYHGRQVQLTYEIPMAEIVLDFFDRLKSVSRGYASMDYEFKEYRASDV
VKVDMLINGDKVDALSVIVHRSQSQYRGREVAAKMREIIPRQMYDVAIQAAIGAHIIARENIKALRKNVLAKCYGGDITR
KKKLLEKQKEGKKRMKQVGSVEIPQEAFLAILRVEDK
>Mature_597_residues
MDHIRNFSIIAHIDHGKSTLADRIIQVCGGLTDREMEAQVLDSMDLERERGITIKAQTAALTYRARDGKVYNLNLIDTPG
HVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVEVVPVLNKIDLPAANPDNAITEIEDVIGIDATDATR
CSAKTGLGVEDVLESLIAKVPPPKGDPDAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIKLMATGAQFPVEHIGVF
TPKSRNLESLSAGQVGFIIAGIKELTAAKVGDTVTHVTKAAAEPLPGFKEVKPQVFAGLYPVEANQYDALRESLEKLKLN
DASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVVQSDGTTIMVENPAKMPEPARIEEIR
EPIVTVNLYMPQDYVGSVITLCTQKRGSQINMQYHGRQVQLTYEIPMAEIVLDFFDRLKSVSRGYASMDYEFKEYRASDV
VKVDMLINGDKVDALSVIVHRSQSQYRGREVAAKMREIIPRQMYDVAIQAAIGAHIIARENIKALRKNVLAKCYGGDITR
KKKLLEKQKEGKKRMKQVGSVEIPQEAFLAILRVEDK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=604, Percent_Identity=48.5099337748344, Blast_Score=609, Evalue=1e-174,
Organism=Homo sapiens, GI25306287, Length=452, Percent_Identity=29.2035398230088, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=45.1127819548872, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI18390331, Length=160, Percent_Identity=38.75, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI25306283, Length=149, Percent_Identity=46.3087248322148, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI19923640, Length=149, Percent_Identity=46.3087248322148, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=41.6666666666667, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=42.7272727272727, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=42.7272727272727, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=42.7272727272727, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=33.0827067669173, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=33.0827067669173, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI53729339, Length=218, Percent_Identity=29.3577981651376, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI53729337, Length=218, Percent_Identity=29.3577981651376, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=69.8653198653199, Blast_Score=852, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=503, Percent_Identity=28.8270377733598, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=36.7741935483871, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=32.6923076923077, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789559, Length=228, Percent_Identity=28.9473684210526, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=39.5424836601307, Blast_Score=470, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17556745, Length=465, Percent_Identity=26.2365591397849, Blast_Score=115, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17533571, Length=161, Percent_Identity=37.2670807453416, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=230, Percent_Identity=31.7391304347826, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=38.0597014925373, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=38.0597014925373, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=34.5864661654135, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI32566303, Length=244, Percent_Identity=28.6885245901639, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=47.6744186046512, Blast_Score=576, Evalue=1e-165,
Organism=Saccharomyces cerevisiae, GI6323098, Length=160, Percent_Identity=39.375, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=41.6666666666667, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=41.6666666666667, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=41.7391304347826, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=38.1944444444444, Blast_Score=81, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325337, Length=156, Percent_Identity=32.0512820512821, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6319594, Length=156, Percent_Identity=32.0512820512821, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=333, Percent_Identity=25.5255255255255, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI78706572, Length=602, Percent_Identity=46.0132890365449, Blast_Score=550, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=37.888198757764, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=37.8378378378378, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=37.8378378378378, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=37.8378378378378, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI28574573, Length=137, Percent_Identity=43.0656934306569, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.9261744966443, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=34.5864661654135, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI45553807, Length=278, Percent_Identity=26.978417266187, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI45553816, Length=278, Percent_Identity=26.978417266187, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24651721, Length=278, Percent_Identity=26.978417266187, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17864154, Length=278, Percent_Identity=26.978417266187, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24652838, Length=156, Percent_Identity=35.2564102564103, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17137572, Length=156, Percent_Identity=35.2564102564103, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66050; Mature: 66050

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHIRNFSIIAHIDHGKSTLADRIIQVCGGLTDREMEAQVLDSMDLERERGITIKAQTAA
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCEEEEEEEE
LTYRARDGKVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAI
EEEEECCCEEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
ELGVEVVPVLNKIDLPAANPDNAITEIEDVIGIDATDATRCSAKTGLGVEDVLESLIAKV
HHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
PPPKGDPDAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIKLMATGAQFPVEHIGVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHCCEE
TPKSRNLESLSAGQVGFIIAGIKELTAAKVGDTVTHVTKAAAEPLPGFKEVKPQVFAGLY
CCCCCCCCCCCCCCHHHEEHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHCCHHHHHCCC
PVEANQYDALRESLEKLKLNDASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD
CCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
MDLITTAPTVVYEVVQSDGTTIMVENPAKMPEPARIEEIREPIVTVNLYMPQDYVGSVIT
CHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHCCCEEEEEEECCHHHHHHHHH
LCTQKRGSQINMQYHGRQVQLTYEIPMAEIVLDFFDRLKSVSRGYASMDYEFKEYRASDV
HHHHCCCCEEEEEECCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCE
VKVDMLINGDKVDALSVIVHRSQSQYRGREVAAKMREIIPRQMYDVAIQAAIGAHIIARE
EEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIKALRKNVLAKCYGGDITRKKKLLEKQKEGKKRMKQVGSVEIPQEAFLAILRVEDK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCC
>Mature Secondary Structure
MDHIRNFSIIAHIDHGKSTLADRIIQVCGGLTDREMEAQVLDSMDLERERGITIKAQTAA
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCEEEEEEEE
LTYRARDGKVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAI
EEEEECCCEEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
ELGVEVVPVLNKIDLPAANPDNAITEIEDVIGIDATDATRCSAKTGLGVEDVLESLIAKV
HHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
PPPKGDPDAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIKLMATGAQFPVEHIGVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHCCEE
TPKSRNLESLSAGQVGFIIAGIKELTAAKVGDTVTHVTKAAAEPLPGFKEVKPQVFAGLY
CCCCCCCCCCCCCCHHHEEHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHCCHHHHHCCC
PVEANQYDALRESLEKLKLNDASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD
CCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
MDLITTAPTVVYEVVQSDGTTIMVENPAKMPEPARIEEIREPIVTVNLYMPQDYVGSVIT
CHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHCCCEEEEEEECCHHHHHHHHH
LCTQKRGSQINMQYHGRQVQLTYEIPMAEIVLDFFDRLKSVSRGYASMDYEFKEYRASDV
HHHHCCCCEEEEEECCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCE
VKVDMLINGDKVDALSVIVHRSQSQYRGREVAAKMREIIPRQMYDVAIQAAIGAHIIARE
EEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIKALRKNVLAKCYGGDITRKKKLLEKQKEGKKRMKQVGSVEIPQEAFLAILRVEDK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA