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The map label for this gene is ycgF [H]

Identifier: 238025426

GI number: 238025426

Start: 2620360

End: 2621166

Strand: Reverse

Name: ycgF [H]

Synonym: bglu_2g21160

Alternate gene names: 238025426

Gene position: 2621166-2620360 (Counterclockwise)

Preceding gene: 238025429

Following gene: 238025425

Centisome position: 92.71

GC content: 65.55

Gene sequence:

>807_bases
ATGAACGACAAACCCATCCTCATCCATCCGGCGGACGGCGCCGTGCCCGCCCAATGCACGGGCTGCCGGACCCCGCCCGG
TCAATTCGGGAAACTCAGGTTTGCGTTCCAGCCGATCGTCGACACCGGCACCGGCGCCGCCTATGCCTACGAGGCGCTGG
TGCGCGGTCCCAACGGCGAGCCGGCCGCGGCGGTGCTGGAGACGGTCGACGAGGCGAGCCGCTACCATTTCGATCAACGC
TGCCGGACCTCGGCCATCGAACAGGCCGCGGCGCTGAACATCGACGTGCATCTGTCGATCAATTTCATGCCGAACGCCGT
CTATCGGCCCGAAGCCTGCATCCGCAGTACGCTCGAGGCCGCCGAGAAATTCCAGTTTCCGAGCGATCGCATCATTTTCG
AGACGGTCGAGGGCGAGAACATCCTCGGCCGCTCGCATCTCGTGGATATTTTCCGCTCCTATCGCAGCTTCGGGTTTCAG
ACCGCCATCGACGATTTCGGCGCGGGCTTTTCCGGGCTGAAGCTGCTGGCCGACTTCCAGCCCGATCTGATCAAGCTGGA
CATGGATCTGGTGCGCGGCGTCGACACCAACGCCGCCCGGCAAAGCATCGTGCGTGGCGTGGTGTCGATGTGCCGCGAGC
TGGACATCCGCGTCATCGCCGAAGGCGTCGAGACGCTCGGCGAGCGCGATTTCTTCACGGCGCACGGCGTCTCGCTGATG
CAGGGCTATCTGTTCGCGAAGCCCGCGTTCCATTCGACGCCCGAGCCCGGCGCCGAGTGGCTGGCCGGGGCCGCGCGACT
GTCCTGA

Upstream 100 bases:

>100_bases
TCCGCGCCGGGCCGCCGATACGGGTACGATCAAGCTTGTTGCGTTTCCGGCCGATAAGACGGAGAGCAGCACGCCAGTTT
GGCGTGTCACCGGAGATTGC

Downstream 100 bases:

>100_bases
CCGGGCGGGGCGGCTCTGCCGCTCGGGCGATGATTTTTTTTGGGGCGCGACCCCTTAAGGTGCCGGAACCTCGCGGCCGA
GGCCGACGAGGGCGCGTAGC

Product: diguanylate phosphodiesterase

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNDKPILIHPADGAVPAQCTGCRTPPGQFGKLRFAFQPIVDTGTGAAYAYEALVRGPNGEPAAAVLETVDEASRYHFDQR
CRTSAIEQAAALNIDVHLSINFMPNAVYRPEACIRSTLEAAEKFQFPSDRIIFETVEGENILGRSHLVDIFRSYRSFGFQ
TAIDDFGAGFSGLKLLADFQPDLIKLDMDLVRGVDTNAARQSIVRGVVSMCRELDIRVIAEGVETLGERDFFTAHGVSLM
QGYLFAKPAFHSTPEPGAEWLAGAARLS

Sequences:

>Translated_268_residues
MNDKPILIHPADGAVPAQCTGCRTPPGQFGKLRFAFQPIVDTGTGAAYAYEALVRGPNGEPAAAVLETVDEASRYHFDQR
CRTSAIEQAAALNIDVHLSINFMPNAVYRPEACIRSTLEAAEKFQFPSDRIIFETVEGENILGRSHLVDIFRSYRSFGFQ
TAIDDFGAGFSGLKLLADFQPDLIKLDMDLVRGVDTNAARQSIVRGVVSMCRELDIRVIAEGVETLGERDFFTAHGVSLM
QGYLFAKPAFHSTPEPGAEWLAGAARLS
>Mature_268_residues
MNDKPILIHPADGAVPAQCTGCRTPPGQFGKLRFAFQPIVDTGTGAAYAYEALVRGPNGEPAAAVLETVDEASRYHFDQR
CRTSAIEQAAALNIDVHLSINFMPNAVYRPEACIRSTLEAAEKFQFPSDRIIFETVEGENILGRSHLVDIFRSYRSFGFQ
TAIDDFGAGFSGLKLLADFQPDLIKLDMDLVRGVDTNAARQSIVRGVVSMCRELDIRVIAEGVETLGERDFFTAHGVSLM
QGYLFAKPAFHSTPEPGAEWLAGAARLS

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=222, Percent_Identity=32.8828828828829, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1790496, Length=241, Percent_Identity=26.5560165975104, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787541, Length=228, Percent_Identity=29.8245614035088, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI226510982, Length=229, Percent_Identity=27.9475982532751, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081845, Length=223, Percent_Identity=28.6995515695067, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788502, Length=226, Percent_Identity=27.8761061946903, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081980, Length=239, Percent_Identity=25.1046025104602, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081743, Length=237, Percent_Identity=24.8945147679325, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082096, Length=150, Percent_Identity=28.6666666666667, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788849, Length=150, Percent_Identity=28.6666666666667, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87081921, Length=232, Percent_Identity=25.4310344827586, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786507, Length=120, Percent_Identity=32.5, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787055, Length=232, Percent_Identity=29.7413793103448, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 29258; Mature: 29258

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKPILIHPADGAVPAQCTGCRTPPGQFGKLRFAFQPIVDTGTGAAYAYEALVRGPNGE
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECHHCCCCCHHHHHHHHHCCCCCC
PAAAVLETVDEASRYHFDQRCRTSAIEQAAALNIDVHLSINFMPNAVYRPEACIRSTLEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCHHHHHHHHHH
AEKFQFPSDRIIFETVEGENILGRSHLVDIFRSYRSFGFQTAIDDFGAGFSGLKLLADFQ
HHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCC
PDLIKLDMDLVRGVDTNAARQSIVRGVVSMCRELDIRVIAEGVETLGERDFFTAHGVSLM
CCCEEECHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHH
QGYLFAKPAFHSTPEPGAEWLAGAARLS
HHHHHCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNDKPILIHPADGAVPAQCTGCRTPPGQFGKLRFAFQPIVDTGTGAAYAYEALVRGPNGE
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECHHCCCCCHHHHHHHHHCCCCCC
PAAAVLETVDEASRYHFDQRCRTSAIEQAAALNIDVHLSINFMPNAVYRPEACIRSTLEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCHHHHHHHHHH
AEKFQFPSDRIIFETVEGENILGRSHLVDIFRSYRSFGFQTAIDDFGAGFSGLKLLADFQ
HHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCC
PDLIKLDMDLVRGVDTNAARQSIVRGVVSMCRELDIRVIAEGVETLGERDFFTAHGVSLM
CCCEEECHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHH
QGYLFAKPAFHSTPEPGAEWLAGAARLS
HHHHHCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]