| Definition | Burkholderia glumae BGR1 chromosome chromosome 2, complete sequence. |
|---|---|
| Accession | NC_012721 |
| Length | 2,827,333 |
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The map label for this gene is gshA [H]
Identifier: 238025298
GI number: 238025298
Start: 2450389
End: 2452053
Strand: Reverse
Name: gshA [H]
Synonym: bglu_2g19720
Alternate gene names: 238025298
Gene position: 2452053-2450389 (Counterclockwise)
Preceding gene: 238025299
Following gene: 238025297
Centisome position: 86.73
GC content: 70.63
Gene sequence:
>1665_bases ATGACGACGCCATACCGTCGCCGGCTCTGCGTGCTGAGCGACGCCGCGCACCTGCCGCTGCTCGGCCACGGGCGGTGCGG CGTCGAGCGCGAGGCGCTGCGCGTGGATGCCGCCGGCCGGCTCGCGCTGACGCAGCATCCGCGCGCGCTCGGCGCGGCGC TCACGCATCCGTCGATCACGACCGACTACGCGGAGCCGCTGCTCGAGTTCGTGACGCCGCCGCAGCACGACGCGGCCGAC GTGATCGAGCGGCTCGACGAGCTGCACCGCTTCGCCTGCCAGGCGATCGGCGACGAGCTGCTGTGGAGCCAGTCGATGCC GTGCCCGCTCGACGACGACCGCCGCATTCCGATCGCCTGGTACGGCACGTCGAACCCCGGCATCCTGAAGCACGTATACC GGCGCGGGCTGGCATTGCGCTACGGCAAGGCGATGCAGTGCATCGCCGGAATTCACTACAACTATTCGCTCGCCGATTCG CTGTGGCCGCTGCTGCGCGAGGTGCGCGACGGGTGCGCGCCGGCCGACCGCGACACGCGCTCGACCGGCTATCTCGCGCT GATCCGCAATTTCCATCGCTACGGCTGGCTGCTGCTCTACCTGTTCGGCGCGTCGCCTGCGCTGTGCTCGGGCTTCGTCG CCGGCGACCGGCACGGGCTCGAGGCGTTCGACGCGGCCACGCTGTACCTGCCCTACGCGACCAGCCTGCGCATGAGCGAC CTCGGCTACCAGAACCGCGTGCAGGAGGCGGTCGCGCCGTGCCTGAACGACCTCGACGCCTATCTGGAGGGGCTCGCGCG CGCAGTCGCGCAGCCCTGTCCTGAATATCAGGCCGTCGGCGCGAAGCGCGACGGCGAGTGGCTGCAACTCAACACGCATC TGCTGCAGATGGAGAACGAATTCTATTCGTCGATTTGTCCCAAGCGCACGATGCGCAGCGGCGAGCGGCTGCTCGACGCG CTGCGCAGCGGCGGCATCGAATACGTGGAAGTGCGCAGCCTCGATATCGATCCGTTCGCGCCGCATGGCGTCGCGGAGGC GACGCTGCGCTTCGTCGACGCGTTCCTGCTGTTCTGCGCGCTCGACAACAGCCCGTGCCTCGCCGCCGGGGAGTCTGACA TCTGCAGGCGCAACTTCGCGGCGGTCGCGCGGGAAGGGCGGCGCCCGGGGCTGACGCTGCAATGCGGCGAGCAGCGCGTC GCACTGCGCAGCTGGGCCCGGGAGCTGCTCGCGCGCATACACGAGGCCGCCTGCGTGCTGGACCAGCAGCGCGGCCACAC GCTTGCGACCGCCGCGCTCGACGCGCAGCGCGCCAAGCTCGACGATCCCCAGGCGACGCCGTCGGCACGGGTGCTGGAGG AGCTGCGCAGCTTGCGCAACGAACCGCGCGGTGCCTGCTTCGCGTTCGGGATGCGGCAGTCGCTCGCCCACACCGAGGCG TTTCGCGCGCGGCCGCTGCCGGCGCAGGTGCAGGCCCGCTTCGAGGCGGCCGCCGCCGAATCGCTGCGCCGGCAGGCCGA GCTGGAGCGCACCCAGATCGGCAACTTCGACGCGTTTCTCGCCGCGTACCAGGCGGGAACGCTGAACCAGGAATGGGGCG AGCCCAGTCACGACGCACTGACGCGGCAGCCGCCGCATGCAACCGATCTCACGGAGGATAGATGA
Upstream 100 bases:
>100_bases CGGCCTGCCGCGGCCCAAGAGCCCGCTCGATTGAACGGCGCGCCGCCAGACATCGTTCCCGGAGACCGCGATGACCCATG ATTCACCTCGACAGACGTGG
Downstream 100 bases:
>100_bases GCAGTATCGCGGAATTGAAAACCGAGCGCGGCTTCTCGATGCGCGCAGCGGGCGCGGGCGCGCTGGCGTCGTTCGTCGGC TTCGCCTCGACATTCACGCT
Product: glutamate--cysteine ligase
Products: NA
Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]
Number of amino acids: Translated: 554; Mature: 553
Protein sequence:
>554_residues MTTPYRRRLCVLSDAAHLPLLGHGRCGVEREALRVDAAGRLALTQHPRALGAALTHPSITTDYAEPLLEFVTPPQHDAAD VIERLDELHRFACQAIGDELLWSQSMPCPLDDDRRIPIAWYGTSNPGILKHVYRRGLALRYGKAMQCIAGIHYNYSLADS LWPLLREVRDGCAPADRDTRSTGYLALIRNFHRYGWLLLYLFGASPALCSGFVAGDRHGLEAFDAATLYLPYATSLRMSD LGYQNRVQEAVAPCLNDLDAYLEGLARAVAQPCPEYQAVGAKRDGEWLQLNTHLLQMENEFYSSICPKRTMRSGERLLDA LRSGGIEYVEVRSLDIDPFAPHGVAEATLRFVDAFLLFCALDNSPCLAAGESDICRRNFAAVAREGRRPGLTLQCGEQRV ALRSWARELLARIHEAACVLDQQRGHTLATAALDAQRAKLDDPQATPSARVLEELRSLRNEPRGACFAFGMRQSLAHTEA FRARPLPAQVQARFEAAAAESLRRQAELERTQIGNFDAFLAAYQAGTLNQEWGEPSHDALTRQPPHATDLTEDR
Sequences:
>Translated_554_residues MTTPYRRRLCVLSDAAHLPLLGHGRCGVEREALRVDAAGRLALTQHPRALGAALTHPSITTDYAEPLLEFVTPPQHDAAD VIERLDELHRFACQAIGDELLWSQSMPCPLDDDRRIPIAWYGTSNPGILKHVYRRGLALRYGKAMQCIAGIHYNYSLADS LWPLLREVRDGCAPADRDTRSTGYLALIRNFHRYGWLLLYLFGASPALCSGFVAGDRHGLEAFDAATLYLPYATSLRMSD LGYQNRVQEAVAPCLNDLDAYLEGLARAVAQPCPEYQAVGAKRDGEWLQLNTHLLQMENEFYSSICPKRTMRSGERLLDA LRSGGIEYVEVRSLDIDPFAPHGVAEATLRFVDAFLLFCALDNSPCLAAGESDICRRNFAAVAREGRRPGLTLQCGEQRV ALRSWARELLARIHEAACVLDQQRGHTLATAALDAQRAKLDDPQATPSARVLEELRSLRNEPRGACFAFGMRQSLAHTEA FRARPLPAQVQARFEAAAAESLRRQAELERTQIGNFDAFLAAYQAGTLNQEWGEPSHDALTRQPPHATDLTEDR >Mature_553_residues TTPYRRRLCVLSDAAHLPLLGHGRCGVEREALRVDAAGRLALTQHPRALGAALTHPSITTDYAEPLLEFVTPPQHDAADV IERLDELHRFACQAIGDELLWSQSMPCPLDDDRRIPIAWYGTSNPGILKHVYRRGLALRYGKAMQCIAGIHYNYSLADSL WPLLREVRDGCAPADRDTRSTGYLALIRNFHRYGWLLLYLFGASPALCSGFVAGDRHGLEAFDAATLYLPYATSLRMSDL GYQNRVQEAVAPCLNDLDAYLEGLARAVAQPCPEYQAVGAKRDGEWLQLNTHLLQMENEFYSSICPKRTMRSGERLLDAL RSGGIEYVEVRSLDIDPFAPHGVAEATLRFVDAFLLFCALDNSPCLAAGESDICRRNFAAVAREGRRPGLTLQCGEQRVA LRSWARELLARIHEAACVLDQQRGHTLATAALDAQRAKLDDPQATPSARVLEELRSLRNEPRGACFAFGMRQSLAHTEAF RARPLPAQVQARFEAAAAESLRRQAELERTQIGNFDAFLAAYQAGTLNQEWGEPSHDALTRQPPHATDLTEDR
Specific function: Glutathione biosynthesis; first step. [C]
COG id: COG2918
COG function: function code H; Gamma-glutamylcysteine synthetase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]
Homologues:
Organism=Escherichia coli, GI1789044, Length=498, Percent_Identity=43.3734939759036, Blast_Score=393, Evalue=1e-110,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR007370 - InterPro: IPR006334 [H]
Pfam domain/function: PF04262 Glu_cys_ligase [H]
EC number: =6.3.2.2 [H]
Molecular weight: Translated: 61553; Mature: 61422
Theoretical pI: Translated: 6.55; Mature: 6.55
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.9 %Cys (Translated Protein) 1.3 %Met (Translated Protein) 4.2 %Cys+Met (Translated Protein) 2.9 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 4.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTTPYRRRLCVLSDAAHLPLLGHGRCGVEREALRVDAAGRLALTQHPRALGAALTHPSIT CCCCHHHHHHEECCCCCCCCEECCCCCCCHHHHEECCCCCEEECCCCHHHHHHHCCCCCC TDYAEPLLEFVTPPQHDAADVIERLDELHRFACQAIGDELLWSQSMPCPLDDDRRIPIAW HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE YGTSNPGILKHVYRRGLALRYGKAMQCIAGIHYNYSLADSLWPLLREVRDGCAPADRDTR ECCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC STGYLALIRNFHRYGWLLLYLFGASPALCSGFVAGDRHGLEAFDAATLYLPYATSLRMSD CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHCCCEEEECCCCCCHHHH LGYQNRVQEAVAPCLNDLDAYLEGLARAVAQPCPEYQAVGAKRDGEWLQLNTHLLQMENE CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEEHHHHHHHHHH FYSSICPKRTMRSGERLLDALRSGGIEYVEVRSLDIDPFAPHGVAEATLRFVDAFLLFCA HHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH LDNSPCLAAGESDICRRNFAAVAREGRRPGLTLQCGEQRVALRSWARELLARIHEAACVL CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHH DQQRGHTLATAALDAQRAKLDDPQATPSARVLEELRSLRNEPRGACFAFGMRQSLAHTEA HHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH FRARPLPAQVQARFEAAAAESLRRQAELERTQIGNFDAFLAAYQAGTLNQEWGEPSHDAL HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHC TRQPPHATDLTEDR CCCCCCCCCCCCCC >Mature Secondary Structure TTPYRRRLCVLSDAAHLPLLGHGRCGVEREALRVDAAGRLALTQHPRALGAALTHPSIT CCCHHHHHHEECCCCCCCCEECCCCCCCHHHHEECCCCCEEECCCCHHHHHHHCCCCCC TDYAEPLLEFVTPPQHDAADVIERLDELHRFACQAIGDELLWSQSMPCPLDDDRRIPIAW HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE YGTSNPGILKHVYRRGLALRYGKAMQCIAGIHYNYSLADSLWPLLREVRDGCAPADRDTR ECCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC STGYLALIRNFHRYGWLLLYLFGASPALCSGFVAGDRHGLEAFDAATLYLPYATSLRMSD CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHCCCEEEECCCCCCHHHH LGYQNRVQEAVAPCLNDLDAYLEGLARAVAQPCPEYQAVGAKRDGEWLQLNTHLLQMENE CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEEHHHHHHHHHH FYSSICPKRTMRSGERLLDALRSGGIEYVEVRSLDIDPFAPHGVAEATLRFVDAFLLFCA HHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH LDNSPCLAAGESDICRRNFAAVAREGRRPGLTLQCGEQRVALRSWARELLARIHEAACVL CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHH DQQRGHTLATAALDAQRAKLDDPQATPSARVLEELRSLRNEPRGACFAFGMRQSLAHTEA HHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH FRARPLPAQVQARFEAAAAESLRRQAELERTQIGNFDAFLAAYQAGTLNQEWGEPSHDAL HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHC TRQPPHATDLTEDR CCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA