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The map label for this gene is azoR4 [H]

Identifier: 238025239

GI number: 238025239

Start: 2384635

End: 2385288

Strand: Reverse

Name: azoR4 [H]

Synonym: bglu_2g19110

Alternate gene names: 238025239

Gene position: 2385288-2384635 (Counterclockwise)

Preceding gene: 238025240

Following gene: 238025234

Centisome position: 84.37

GC content: 70.49

Gene sequence:

>654_bases
ATGAAGCTGCTGCTGCTCAATGCGAGCCCGTTCGGCGAGGCGAGCCACGCCTACGGGCTCGCGCGCGAGACGGTGGACGC
GCTGCGCCGCCAGGAGGCCGCCGATGGCCGCGAATTCACGCTGATCGAGCGCGACCTGGCGGCCACGCCGCTGCCGCCCA
TCACGCCCGCCTACGCGAGCGCGATCACCTCGCGCACGCCCGATGCCGCGCAGTTCGCGCTGTCGGAGGAACTGGTGCTC
GAGATCGAGGCAACCGACTGCCTCGTGATCGCCACACCGATGCACAACTTCACCGTGCCGGCCGCGCTCAAGCTCTGGAT
CGACTACGTGCTGCGAATCAACCGCACGTTCGCGGGTACGCCGGAAGGCAAGGTGGGGCTGATGCAGGACCGGCCGACCT
TCGTGATCGTGGGCTCGGGCGGCATACACAGCGGCCCGAACGCGCGCCAGACCGATTTCCTCACGCCCTACCTCGGCTAC
GCGCTCGGCACGATCGGGATCCGCAACGTGCGTTTCTTCCTGCTGCAAGGGCTGGTGATGGGCGAGGCGGCGGTGCAGGC
CGAACTCGCGCGCGAACGCGCCGCGCTGCGCCGCTGCGTGCCGTTCTCGGCGCTGCCCGCGAGCGACTCCGGGCAGCCGG
CCGCGGCCAGCTGA

Upstream 100 bases:

>100_bases
TGCACATGCCGCTCGCGCAACGTTTCTATTTTCGCAACGGCATGCTCGCGAAAGGCATGCATTTCGTCGAGCCGCTGAGC
GCCCTGGAGACGACTGCACG

Downstream 100 bases:

>100_bases
GGCCGCGGGCGCCGGGCGATGGCGCACTCGCGCGGTCCGTGCGGGGCCGCCGCTGCGCAGCGCGGGGCGTCGGCTTCGAG
CCGCGCCCGGCCCGGTTTCG

Product: acyl carrier protein phosphodiesterase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 4; FMN-dependent NADH-azo compound oxidoreductase 4 [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MKLLLLNASPFGEASHAYGLARETVDALRRQEAADGREFTLIERDLAATPLPPITPAYASAITSRTPDAAQFALSEELVL
EIEATDCLVIATPMHNFTVPAALKLWIDYVLRINRTFAGTPEGKVGLMQDRPTFVIVGSGGIHSGPNARQTDFLTPYLGY
ALGTIGIRNVRFFLLQGLVMGEAAVQAELARERAALRRCVPFSALPASDSGQPAAAS

Sequences:

>Translated_217_residues
MKLLLLNASPFGEASHAYGLARETVDALRRQEAADGREFTLIERDLAATPLPPITPAYASAITSRTPDAAQFALSEELVL
EIEATDCLVIATPMHNFTVPAALKLWIDYVLRINRTFAGTPEGKVGLMQDRPTFVIVGSGGIHSGPNARQTDFLTPYLGY
ALGTIGIRNVRFFLLQGLVMGEAAVQAELARERAALRRCVPFSALPASDSGQPAAAS
>Mature_217_residues
MKLLLLNASPFGEASHAYGLARETVDALRRQEAADGREFTLIERDLAATPLPPITPAYASAITSRTPDAAQFALSEELVL
EIEATDCLVIATPMHNFTVPAALKLWIDYVLRINRTFAGTPEGKVGLMQDRPTFVIVGSGGIHSGPNARQTDFLTPYLGY
ALGTIGIRNVRFFLLQGLVMGEAAVQAELARERAALRRCVPFSALPASDSGQPAAAS

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=208, Percent_Identity=37.5, Blast_Score=108, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 23266; Mature: 23266

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLLLNASPFGEASHAYGLARETVDALRRQEAADGREFTLIERDLAATPLPPITPAYAS
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHH
AITSRTPDAAQFALSEELVLEIEATDCLVIATPMHNFTVPAALKLWIDYVLRINRTFAGT
HHHCCCCCHHHHHHCCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCC
PEGKVGLMQDRPTFVIVGSGGIHSGPNARQTDFLTPYLGYALGTIGIRNVRFFLLQGLVM
CCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GEAAVQAELARERAALRRCVPFSALPASDSGQPAAAS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLLLLNASPFGEASHAYGLARETVDALRRQEAADGREFTLIERDLAATPLPPITPAYAS
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHH
AITSRTPDAAQFALSEELVLEIEATDCLVIATPMHNFTVPAALKLWIDYVLRINRTFAGT
HHHCCCCCHHHHHHCCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCC
PEGKVGLMQDRPTFVIVGSGGIHSGPNARQTDFLTPYLGYALGTIGIRNVRFFLLQGLVM
CCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GEAAVQAELARERAALRRCVPFSALPASDSGQPAAAS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA