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The map label for this gene is thiD [H]

Identifier: 238025093

GI number: 238025093

Start: 2195167

End: 2195964

Strand: Reverse

Name: thiD [H]

Synonym: bglu_2g17500

Alternate gene names: 238025093

Gene position: 2195964-2195167 (Counterclockwise)

Preceding gene: 238025094

Following gene: 238025092

Centisome position: 77.67

GC content: 72.18

Gene sequence:

>798_bases
ATGATCGCCAACGTGCTCAGCATCGCCGGCACCGATCCGACCGGCGGCGCCGGCATCCAGGCCGACCTGAAGACGTTCTC
GGCGCTCGGCGCCTACGGCATGTCGGCCATCACGGCGGTGGTCGCGCAGAACACGCGCGGCGTGCGCGGCTTTCATACGC
TCGAGCCGGCCTTCATCGTCGAGCAGATCGACGCCGTGTTCGACGACGTGACCGTGCATGCCGTGAAGCTCGGCATGCTC
GCCACCGCCGCGATTGCGGAGGCGGTGGCCGAGGCGCTGGTGCGCCATCGGGCAACGACCGTGGTACTCGATCCCGTGAT
GGTGGCCAAGAGCGGCGACCGGCTGCTCGCGCCCGACGCGGTGGCGGCGATCCGCGAGCGGCTGGTGCCGCTCGCCACGC
TGATCACGCCGAACCTGCCCGAGGCGGGCGTCCTGCTCGACCGGCCGGCGCCTGCCTCGCTGGCGGAAATGCACGATGCC
CTGCAGGCGCTGCATGGGCTCGGCCCGCGCTGGGTGCTACTCAAGGGCGGCCATCTGCGGGGCGCGGACAGCACCGACCT
GCTGTTCGGCGCGAGCGGCACCACCGAGCTGGCCGCGCCGCGCATCGCGACGCGCAACGATCACGGCACCGGCTGCACGC
TGTCGGCGGCGGTGGCCGCGCTGCTGCCGCATCACCCGATGGAGGAGAGCGTGCGGCGCGCGAAGGCCTATCTGAATCGC
GCGCTCGCCGAGTCGTGGCGGCTCGAAGTCGGCCACGGCCGCGGGCCGACCCACCATTTTCACGCGCTCTGGCGCTAG

Upstream 100 bases:

>100_bases
GGCCGCGCGGGCGGCACGCCGCCGGCGCGGCCGCTTCGGCCCGCCTGGTTGCGCGCGCAACCGGAACGCGGCGCGCGCGG
CCGCACCGGGGGCGGGCCCC

Downstream 100 bases:

>100_bases
CGCGCCGGACGACCCCGCCGGGGGGACCGGCGCATCAAAGTCCAAGGAAAAAACATGCGTCGAATGGTTTGCCGCCTCAT
GCTGGCCCTCGGGTGCTGGC

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MIANVLSIAGTDPTGGAGIQADLKTFSALGAYGMSAITAVVAQNTRGVRGFHTLEPAFIVEQIDAVFDDVTVHAVKLGML
ATAAIAEAVAEALVRHRATTVVLDPVMVAKSGDRLLAPDAVAAIRERLVPLATLITPNLPEAGVLLDRPAPASLAEMHDA
LQALHGLGPRWVLLKGGHLRGADSTDLLFGASGTTELAAPRIATRNDHGTGCTLSAAVAALLPHHPMEESVRRAKAYLNR
ALAESWRLEVGHGRGPTHHFHALWR

Sequences:

>Translated_265_residues
MIANVLSIAGTDPTGGAGIQADLKTFSALGAYGMSAITAVVAQNTRGVRGFHTLEPAFIVEQIDAVFDDVTVHAVKLGML
ATAAIAEAVAEALVRHRATTVVLDPVMVAKSGDRLLAPDAVAAIRERLVPLATLITPNLPEAGVLLDRPAPASLAEMHDA
LQALHGLGPRWVLLKGGHLRGADSTDLLFGASGTTELAAPRIATRNDHGTGCTLSAAVAALLPHHPMEESVRRAKAYLNR
ALAESWRLEVGHGRGPTHHFHALWR
>Mature_265_residues
MIANVLSIAGTDPTGGAGIQADLKTFSALGAYGMSAITAVVAQNTRGVRGFHTLEPAFIVEQIDAVFDDVTVHAVKLGML
ATAAIAEAVAEALVRHRATTVVLDPVMVAKSGDRLLAPDAVAAIRERLVPLATLITPNLPEAGVLLDRPAPASLAEMHDA
LQALHGLGPRWVLLKGGHLRGADSTDLLFGASGTTELAAPRIATRNDHGTGCTLSAAVAALLPHHPMEESVRRAKAYLNR
ALAESWRLEVGHGRGPTHHFHALWR

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=262, Percent_Identity=58.3969465648855, Blast_Score=295, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6325378, Length=274, Percent_Identity=31.3868613138686, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6324997, Length=278, Percent_Identity=36.3309352517986, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324517, Length=196, Percent_Identity=37.2448979591837, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27820; Mature: 27820

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIANVLSIAGTDPTGGAGIQADLKTFSALGAYGMSAITAVVAQNTRGVRGFHTLEPAFIV
CCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHH
EQIDAVFDDVTVHAVKLGMLATAAIAEAVAEALVRHRATTVVLDPVMVAKSGDRLLAPDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEECCCCCEECCHH
VAAIRERLVPLATLITPNLPEAGVLLDRPAPASLAEMHDALQALHGLGPRWVLLKGGHLR
HHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEEC
GADSTDLLFGASGTTELAAPRIATRNDHGTGCTLSAAVAALLPHHPMEESVRRAKAYLNR
CCCCCCEEEECCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ALAESWRLEVGHGRGPTHHFHALWR
HHHHHHCEEECCCCCCCHHHHHHCC
>Mature Secondary Structure
MIANVLSIAGTDPTGGAGIQADLKTFSALGAYGMSAITAVVAQNTRGVRGFHTLEPAFIV
CCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHH
EQIDAVFDDVTVHAVKLGMLATAAIAEAVAEALVRHRATTVVLDPVMVAKSGDRLLAPDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEECCCCCEECCHH
VAAIRERLVPLATLITPNLPEAGVLLDRPAPASLAEMHDALQALHGLGPRWVLLKGGHLR
HHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEEC
GADSTDLLFGASGTTELAAPRIATRNDHGTGCTLSAAVAALLPHHPMEESVRRAKAYLNR
CCCCCCEEEECCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ALAESWRLEVGHGRGPTHHFHALWR
HHHHHHCEEECCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]