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The map label for this gene is dnaG [H]

Identifier: 238024933

GI number: 238024933

Start: 2007241

End: 2009127

Strand: Reverse

Name: dnaG [H]

Synonym: bglu_2g15880

Alternate gene names: 238024933

Gene position: 2009127-2007241 (Counterclockwise)

Preceding gene: 238024934

Following gene: 238024932

Centisome position: 71.06

GC content: 66.72

Gene sequence:

>1887_bases
GTGATCCCGCATTCGTTTCTTCAGGACCTGCTCAACCGCGTCGATATCGTCGACGTGGTGGGCCGTTACGTCCAGCTGAA
GAAGGGCGGTGCCAACTTCATGGGGCTCTGCCCGTTCCACAACGAGAAGAGCCCGTCGTTTACCGTCAGCCCGACCAAGC
AGTTCTACCACTGCTTCGGCTGCGGCGCGCACGGCACCGCGATCGGCTTCCTGATGGAACACGCCGGGCTCACGTTTCCC
GAGGCCGTCGAGCAGCTCGCGCAATCGGTCGGGCTGAGCGTGCCGCGCGAGCCCTCGCCGATGCGTGGCGGCGGTGGCTA
CGGCGGCCATGGCGGCGAGGGTGCGCCGCTGCCGGCCGTGTCGAAATCGTTGACCACGGCGCTGACCGACGTGATGCAGA
CCGCCTGCGACTACTATCGCAAGCAGCTGCGCGGCGCGCCGAACGCGATCCAGTATCTGAAGAAGCGCGGCCTGACGGGC
GAGATCGCGCTGCGCTTCGGGCTCGGCTATGCGCCGGACGGCTGGCAGAACCTGGAGGAGGCGTTCGAGGACTACCGGCG
CGAGGAGCTCGTCGAGGCCGGGCTCGTGATCGTCAGCGAGAAGACCGATGCGCAGGGCGCCGCGCGCCGCTACGACCGGT
TCCGCGAGCGCATCATGTTTCCGATCCGCAACGTGAAGGGCGCCGTGATCGGCTTCGGCGGCCGCGTGCTCGACTCCGGC
GAACCGAAGTACCTCAATTCGCCGGAAACGCCGCTCTTCAACAAGGGCAGCGAGTTGTACGGGCTGTTCGAGGCGCGCCT
TGCGATCCGCGAGCACAAGTACGCATTGGTGGTGGAGGGCTACATGGACGTGGTCGCGCTCGCGCAACTCGGTTTCCCGA
ACGCGGTGGCCACGCTCGGCACGGCCTGCACGCCGATCCATGTGCAGAAGCTGCTGCGGCAGACCGATACCGTGGTGTTC
AGCTTCGACGGCGACTCGGCCGGCCGCCGCGCCGCGCGTCGCGCGCTCGAGGCCTGCCTGCCGCACGCGGCCGACAATCG
GACGATCCGCTTCCTGTTCCTGCCGGCCGAGCACGATCCGGACAGCTACGTGCGCGAGTTCGGCGCCGACGCGTTTTCCG
AGCAGGTGCGCCGTGCCATGCCGCTGTCGCAGTTCCTGCTCAACGAGGCGATCCGCGACAAGGAACTCGACCAGCCGGAA
GGGCGGGCCAAGGCCCTGTTCGACGCGAAGCCGCTGCTGCAGGCGCTGCCCGCCAACGCGCTGCGCGCGCAGATCATGCA
TATGTTCGCCGATCGCCTCGACATCCCGTTCGAGGAAGTGGCGAGCCTGAGCGAGGTCGATGCGCGGATTCCCGCGCCGC
TGCGCCAGGCACCCGCGCGCAACGACCGCCGGCGCGTGACCGACCAGGAGAAGCGCGCGCTGCGCAACCTGATCATGCAT
CCGCGCATCGCGATGCTGCTCGACGACGACGAGATCGCCACGCTGCGCGGCTTGCCGAGAAATGGGGAACTGTTCGACGA
GGTGCTCGGCCATGCGCGTGCACTGGGGGATGCGGCTGAATTCCGGCTGTTGCCCGACGTTCTACGCAGTTCGGCGAACG
CGGAAACCTATGACGAAATCATTCGTGAAATTCTCGACTATGATGAAAACGTTCGCGATCTCATGTCGGATCCTGCCGAG
GACGGCGAGGCGCAGGAAACCGTGCGGGAGCGGGAGCGGATCGCGGGCGAGGAATTGAAGGCGGCCGTGCTGAAGATGCG
CTACGACGCCTGTAACGACCGCTGCAACGAGCTGGCGAGGCTTTCGAAGCCGTCCCCGGAGGAGCTGGCCGAACTCGTCG
TACTGAACCGCGCGCGTACCGAAATGAAGGCGCGGCTTGGCCTGTAA

Upstream 100 bases:

>100_bases
GACATGACGGCGGTGGCTGCGCTCGCCAAGGCGGCGCTCGTCAAGTAAGCGAAGGCGGGCACGCACGCCGTGCCCGCCGC
GTTCGACCCAGGCCGCCGTC

Downstream 100 bases:

>100_bases
TGGCGGGGCTGTCCGCAGGCGGCCAATCGGGAAGGAAGTGACTAACGGGGAAGGAAGAGGCTTACCCCAACGTGCTATAA
TACAAGGTTTTCAGCGGTTT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 628; Mature: 628

Protein sequence:

>628_residues
MIPHSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGTAIGFLMEHAGLTFP
EAVEQLAQSVGLSVPREPSPMRGGGGYGGHGGEGAPLPAVSKSLTTALTDVMQTACDYYRKQLRGAPNAIQYLKKRGLTG
EIALRFGLGYAPDGWQNLEEAFEDYRREELVEAGLVIVSEKTDAQGAARRYDRFRERIMFPIRNVKGAVIGFGGRVLDSG
EPKYLNSPETPLFNKGSELYGLFEARLAIREHKYALVVEGYMDVVALAQLGFPNAVATLGTACTPIHVQKLLRQTDTVVF
SFDGDSAGRRAARRALEACLPHAADNRTIRFLFLPAEHDPDSYVREFGADAFSEQVRRAMPLSQFLLNEAIRDKELDQPE
GRAKALFDAKPLLQALPANALRAQIMHMFADRLDIPFEEVASLSEVDARIPAPLRQAPARNDRRRVTDQEKRALRNLIMH
PRIAMLLDDDEIATLRGLPRNGELFDEVLGHARALGDAAEFRLLPDVLRSSANAETYDEIIREILDYDENVRDLMSDPAE
DGEAQETVRERERIAGEELKAAVLKMRYDACNDRCNELARLSKPSPEELAELVVLNRARTEMKARLGL

Sequences:

>Translated_628_residues
MIPHSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGTAIGFLMEHAGLTFP
EAVEQLAQSVGLSVPREPSPMRGGGGYGGHGGEGAPLPAVSKSLTTALTDVMQTACDYYRKQLRGAPNAIQYLKKRGLTG
EIALRFGLGYAPDGWQNLEEAFEDYRREELVEAGLVIVSEKTDAQGAARRYDRFRERIMFPIRNVKGAVIGFGGRVLDSG
EPKYLNSPETPLFNKGSELYGLFEARLAIREHKYALVVEGYMDVVALAQLGFPNAVATLGTACTPIHVQKLLRQTDTVVF
SFDGDSAGRRAARRALEACLPHAADNRTIRFLFLPAEHDPDSYVREFGADAFSEQVRRAMPLSQFLLNEAIRDKELDQPE
GRAKALFDAKPLLQALPANALRAQIMHMFADRLDIPFEEVASLSEVDARIPAPLRQAPARNDRRRVTDQEKRALRNLIMH
PRIAMLLDDDEIATLRGLPRNGELFDEVLGHARALGDAAEFRLLPDVLRSSANAETYDEIIREILDYDENVRDLMSDPAE
DGEAQETVRERERIAGEELKAAVLKMRYDACNDRCNELARLSKPSPEELAELVVLNRARTEMKARLGL
>Mature_628_residues
MIPHSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGTAIGFLMEHAGLTFP
EAVEQLAQSVGLSVPREPSPMRGGGGYGGHGGEGAPLPAVSKSLTTALTDVMQTACDYYRKQLRGAPNAIQYLKKRGLTG
EIALRFGLGYAPDGWQNLEEAFEDYRREELVEAGLVIVSEKTDAQGAARRYDRFRERIMFPIRNVKGAVIGFGGRVLDSG
EPKYLNSPETPLFNKGSELYGLFEARLAIREHKYALVVEGYMDVVALAQLGFPNAVATLGTACTPIHVQKLLRQTDTVVF
SFDGDSAGRRAARRALEACLPHAADNRTIRFLFLPAEHDPDSYVREFGADAFSEQVRRAMPLSQFLLNEAIRDKELDQPE
GRAKALFDAKPLLQALPANALRAQIMHMFADRLDIPFEEVASLSEVDARIPAPLRQAPARNDRRRVTDQEKRALRNLIMH
PRIAMLLDDDEIATLRGLPRNGELFDEVLGHARALGDAAEFRLLPDVLRSSANAETYDEIIREILDYDENVRDLMSDPAE
DGEAQETVRERERIAGEELKAAVLKMRYDACNDRCNELARLSKPSPEELAELVVLNRARTEMKARLGL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=498, Percent_Identity=44.9799196787149, Blast_Score=393, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 69806; Mature: 69806

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPHSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEECCHHHHHHHHC
CGAHGTAIGFLMEHAGLTFPEAVEQLAQSVGLSVPREPSPMRGGGGYGGHGGEGAPLPAV
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
SKSLTTALTDVMQTACDYYRKQLRGAPNAIQYLKKRGLTGEIALRFGLGYAPDGWQNLEE
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEHHEECCCCCCCCHHHHHH
AFEDYRREELVEAGLVIVSEKTDAQGAARRYDRFRERIMFPIRNVKGAVIGFGGRVLDSG
HHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCC
EPKYLNSPETPLFNKGSELYGLFEARLAIREHKYALVVEGYMDVVALAQLGFPNAVATLG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCHHHHHHH
TACTPIHVQKLLRQTDTVVFSFDGDSAGRRAARRALEACLPHAADNRTIRFLFLPAEHDP
CCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCC
DSYVREFGADAFSEQVRRAMPLSQFLLNEAIRDKELDQPEGRAKALFDAKPLLQALPANA
HHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHH
LRAQIMHMFADRLDIPFEEVASLSEVDARIPAPLRQAPARNDRRRVTDQEKRALRNLIMH
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCHHCCCHHHHHHHHHHHHC
PRIAMLLDDDEIATLRGLPRNGELFDEVLGHARALGDAAEFRLLPDVLRSSANAETYDEI
CCEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHH
IREILDYDENVRDLMSDPAEDGEAQETVRERERIAGEELKAAVLKMRYDACNDRCNELAR
HHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSKPSPEELAELVVLNRARTEMKARLGL
CCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPHSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEECCHHHHHHHHC
CGAHGTAIGFLMEHAGLTFPEAVEQLAQSVGLSVPREPSPMRGGGGYGGHGGEGAPLPAV
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
SKSLTTALTDVMQTACDYYRKQLRGAPNAIQYLKKRGLTGEIALRFGLGYAPDGWQNLEE
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEHHEECCCCCCCCHHHHHH
AFEDYRREELVEAGLVIVSEKTDAQGAARRYDRFRERIMFPIRNVKGAVIGFGGRVLDSG
HHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCC
EPKYLNSPETPLFNKGSELYGLFEARLAIREHKYALVVEGYMDVVALAQLGFPNAVATLG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCHHHHHHH
TACTPIHVQKLLRQTDTVVFSFDGDSAGRRAARRALEACLPHAADNRTIRFLFLPAEHDP
CCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCC
DSYVREFGADAFSEQVRRAMPLSQFLLNEAIRDKELDQPEGRAKALFDAKPLLQALPANA
HHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHH
LRAQIMHMFADRLDIPFEEVASLSEVDARIPAPLRQAPARNDRRRVTDQEKRALRNLIMH
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCHHCCCHHHHHHHHHHHHC
PRIAMLLDDDEIATLRGLPRNGELFDEVLGHARALGDAAEFRLLPDVLRSSANAETYDEI
CCEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHH
IREILDYDENVRDLMSDPAEDGEAQETVRERERIAGEELKAAVLKMRYDACNDRCNELAR
HHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSKPSPEELAELVVLNRARTEMKARLGL
CCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]