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The map label for this gene is glgX [H]

Identifier: 238024913

GI number: 238024913

Start: 1975684

End: 1977981

Strand: Direct

Name: glgX [H]

Synonym: bglu_2g15580

Alternate gene names: 238024913

Gene position: 1975684-1977981 (Clockwise)

Preceding gene: 238024912

Following gene: 238024914

Centisome position: 69.88

GC content: 72.11

Gene sequence:

>2298_bases
ATGCGTGCTGCGACCGCCGACCGGCTCGAGCCGGGCAAGCCGTATCCGCTCGGAGCGAGCTGGAACGGCCTCGGCATTAA
TTTCGCGGTGTTTTCCGCGCACGCCACGCGCCTTCAGCTGTGCCTGTTCGACCCCACCGGGCGCAAGGAGATCGCCCGGC
TCGACCTGCCGGAATGCACCGACGAAATCTGGCACGGCTACCTGCCGGGCGCGCATCCCGGCACCGTCTACGCGTTTCGC
GCCGACGGCCCGTACCAGCCGCAGTATGGCCACCGCTTCAATTCCTCGAAGCTGCTGCTCGACCCCTACGCGCGCAAGCT
GATCGGCCAGTTCCGCTGGTCCGACGCGCTGTTCGGCTACCGGCTCCACTCGAACCGCGCCGACCTGTCGATCGACCGCC
GAGATTCGGCGCCGGCCATGCCCAAGTGCGTGGTGGTGGACGAGTCGTTCGACTGGCACGACGACCGCCGCCCCGCGGTG
CCGTGGCAGGACACGGTGATCTACGAGACCCACGTGCGCGGCGTCTCGATGCGCCGCGACGGGCTGCGCACGCCGGAGCG
CGGCACCTTCGCGGTGCTCGCCTCGCCCGATTTCATCGACCATCTGCTCAGGCTCGGCGTGACCACCGTCGAGCTGCTGC
CGGTGCAGGCGTTCCTCGACAGCCGCGAGCTCGTCAACCGCGGGCTGCGCAACTACTGGGGCTACGACACGCTCGCGTAT
TTCGCGCCGCAGCCGTCGTATCTCGCCACGCGCCGGCTCGACGAGATGCGCATCGCGATCCGCCAGCTGCATGCGGCCGG
CATCGAGGTGCTGCTCGACGTGGTCTACAACCACACCTGCGAAGGCAACCAGCTCGGCCCGACGCTGTCGTGGCGCGGCC
TCGACAACGCCAGCTACTACCGGCTGCAGCCGAACGACCCGCGCTACCACATCGACGAGACCGGCTGCGGCAACACGCTG
AACCTGTCGCATCCGCGCGTGCTGCAGATGGTGATGGACTCGCTGCGCTACTGGTGTACCGCCTTCAACATCGACGGCTT
CCGCTTCGACCTCGGCGTCACGCTCGGCCGCGAGCCGCACGGCTATGACCGCGGCTCGGGCTTCTTCGACGCGCTGTGCC
AGGACCCGGTGCTGGCCACCCGCAAGCTGATCTCCGAGCCGTGGGACCTCGGCCCCGGCGGCTACCAGCTCGGCAACCAT
CCGGCCGGCTTCGGCGAATGGAACGACAAGTTCCGCGACACGGTGCGCCGCTTCTGGCGCGGCGACGCCGGCATGCGGCC
CGAGCTGGCCGCGCGGCTGGCCGGCTCGGCCGACCTGTTCAACCACCACCGGCGCAAGCCGTGGGCCTCGGTGAACTTCG
TCACGGCCCACGACGGCTTCACGCTGGCCGACCTCGTCTCGTACTCGTCCAAGCGCAACGAGGCCAACGGCGAGGAGAAC
CGCGACGGCCGCGACGACAACTGCAGCGCGAACTGGGGCGTGGAAGGCCCGACCGACGACGCCGACATCCGCGAGGTGCG
CGAGCGCGTGGCGCGCTCGATGCTGACCACGCTCTACGCCGCGCTCGGCACCCCGATGCTGCTCGGCGGCGACGAGTTCG
GCCGCAGCCAGCAGGGCAACAACAATGCCTATTGCCAGGACACCGAGCTCGCGTGGTTCGACTGGGAGGCCGCCGCCAGC
GACGCCGGGCGCCGGCTCACCGGCTTCGTCGCGCGGCTCGCCGCGCTGCGCCGCGAGCACCCGCTGCTGTCGGGGCCGGG
CTATCCGTCGGGCGACCGCGAGGCCGCGCCGGGGCTGCGCGAGATCGACTGGTTCGACGAGCGCGGCGAGGCGATCCCGG
TGCCCGCCTGGCAGAACCGCGAGCTGCGCGCGCTGATGATGCGCCGCGTCGGGCCCGATCGCGACGGCCACACCGAGGTG
CTGCTGCTGATGCTCAACGGCTCGGCGCAGCCGCTGGTATTCGTGCCGCCCGCGCCGCGTCTGGCGTTCCGCGTGCTGGC
CGACAGCGCGCGGCCCGACCTGGAGCCGCAGCCGCTGCCGGCCGAAGGCATCGAGCTGGCCGCTCACGCGGCCGTGATCG
CGGCCGCAAGGCTGCCCGCCGAGCAGTGCACGCGCCCCGACCACGCGGGTGCGGGCGCCGCCGGCGAGGCGCGCCCCGCG
GACGAGGGCGACGCCCCCCCGGACGACGCCGACCACGCGGCGCAGCCGCAGGCGGCGCCCGCTCCGCAACGGGAGCCCGA
ACCCGAGCCGACCGCCGGCCCCGCGCCGGCCCATGACGAACCCACGCCGGTGTCCTGA

Upstream 100 bases:

>100_bases
GCCACGCTCTGGCTGCGCCCGGCCTGAGCCGCGCCGGTGCGGCGCCGCCCCCGAAACATCGTGATCCGAAACAACACGCG
ACAACGAGGAGAACACCATC

Downstream 100 bases:

>100_bases
GCACGACACCGGCCCGCCTCCGAGACATCGCGATGACCCAATCCCTGATTTCCGCTCCCGGCCGCCACGCGTATCCGACC
TCGTTCGGCGCCACCTGCAT

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 765; Mature: 765

Protein sequence:

>765_residues
MRAATADRLEPGKPYPLGASWNGLGINFAVFSAHATRLQLCLFDPTGRKEIARLDLPECTDEIWHGYLPGAHPGTVYAFR
ADGPYQPQYGHRFNSSKLLLDPYARKLIGQFRWSDALFGYRLHSNRADLSIDRRDSAPAMPKCVVVDESFDWHDDRRPAV
PWQDTVIYETHVRGVSMRRDGLRTPERGTFAVLASPDFIDHLLRLGVTTVELLPVQAFLDSRELVNRGLRNYWGYDTLAY
FAPQPSYLATRRLDEMRIAIRQLHAAGIEVLLDVVYNHTCEGNQLGPTLSWRGLDNASYYRLQPNDPRYHIDETGCGNTL
NLSHPRVLQMVMDSLRYWCTAFNIDGFRFDLGVTLGREPHGYDRGSGFFDALCQDPVLATRKLISEPWDLGPGGYQLGNH
PAGFGEWNDKFRDTVRRFWRGDAGMRPELAARLAGSADLFNHHRRKPWASVNFVTAHDGFTLADLVSYSSKRNEANGEEN
RDGRDDNCSANWGVEGPTDDADIREVRERVARSMLTTLYAALGTPMLLGGDEFGRSQQGNNNAYCQDTELAWFDWEAAAS
DAGRRLTGFVARLAALRREHPLLSGPGYPSGDREAAPGLREIDWFDERGEAIPVPAWQNRELRALMMRRVGPDRDGHTEV
LLLMLNGSAQPLVFVPPAPRLAFRVLADSARPDLEPQPLPAEGIELAAHAAVIAAARLPAEQCTRPDHAGAGAAGEARPA
DEGDAPPDDADHAAQPQAAPAPQREPEPEPTAGPAPAHDEPTPVS

Sequences:

>Translated_765_residues
MRAATADRLEPGKPYPLGASWNGLGINFAVFSAHATRLQLCLFDPTGRKEIARLDLPECTDEIWHGYLPGAHPGTVYAFR
ADGPYQPQYGHRFNSSKLLLDPYARKLIGQFRWSDALFGYRLHSNRADLSIDRRDSAPAMPKCVVVDESFDWHDDRRPAV
PWQDTVIYETHVRGVSMRRDGLRTPERGTFAVLASPDFIDHLLRLGVTTVELLPVQAFLDSRELVNRGLRNYWGYDTLAY
FAPQPSYLATRRLDEMRIAIRQLHAAGIEVLLDVVYNHTCEGNQLGPTLSWRGLDNASYYRLQPNDPRYHIDETGCGNTL
NLSHPRVLQMVMDSLRYWCTAFNIDGFRFDLGVTLGREPHGYDRGSGFFDALCQDPVLATRKLISEPWDLGPGGYQLGNH
PAGFGEWNDKFRDTVRRFWRGDAGMRPELAARLAGSADLFNHHRRKPWASVNFVTAHDGFTLADLVSYSSKRNEANGEEN
RDGRDDNCSANWGVEGPTDDADIREVRERVARSMLTTLYAALGTPMLLGGDEFGRSQQGNNNAYCQDTELAWFDWEAAAS
DAGRRLTGFVARLAALRREHPLLSGPGYPSGDREAAPGLREIDWFDERGEAIPVPAWQNRELRALMMRRVGPDRDGHTEV
LLLMLNGSAQPLVFVPPAPRLAFRVLADSARPDLEPQPLPAEGIELAAHAAVIAAARLPAEQCTRPDHAGAGAAGEARPA
DEGDAPPDDADHAAQPQAAPAPQREPEPEPTAGPAPAHDEPTPVS
>Mature_765_residues
MRAATADRLEPGKPYPLGASWNGLGINFAVFSAHATRLQLCLFDPTGRKEIARLDLPECTDEIWHGYLPGAHPGTVYAFR
ADGPYQPQYGHRFNSSKLLLDPYARKLIGQFRWSDALFGYRLHSNRADLSIDRRDSAPAMPKCVVVDESFDWHDDRRPAV
PWQDTVIYETHVRGVSMRRDGLRTPERGTFAVLASPDFIDHLLRLGVTTVELLPVQAFLDSRELVNRGLRNYWGYDTLAY
FAPQPSYLATRRLDEMRIAIRQLHAAGIEVLLDVVYNHTCEGNQLGPTLSWRGLDNASYYRLQPNDPRYHIDETGCGNTL
NLSHPRVLQMVMDSLRYWCTAFNIDGFRFDLGVTLGREPHGYDRGSGFFDALCQDPVLATRKLISEPWDLGPGGYQLGNH
PAGFGEWNDKFRDTVRRFWRGDAGMRPELAARLAGSADLFNHHRRKPWASVNFVTAHDGFTLADLVSYSSKRNEANGEEN
RDGRDDNCSANWGVEGPTDDADIREVRERVARSMLTTLYAALGTPMLLGGDEFGRSQQGNNNAYCQDTELAWFDWEAAAS
DAGRRLTGFVARLAALRREHPLLSGPGYPSGDREAAPGLREIDWFDERGEAIPVPAWQNRELRALMMRRVGPDRDGHTEV
LLLMLNGSAQPLVFVPPAPRLAFRVLADSARPDLEPQPLPAEGIELAAHAAVIAAARLPAEQCTRPDHAGAGAAGEARPA
DEGDAPPDDADHAAQPQAAPAPQREPEPEPTAGPAPAHDEPTPVS

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=642, Percent_Identity=46.417445482866, Blast_Score=543, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 84920; Mature: 84920

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAATADRLEPGKPYPLGASWNGLGINFAVFSAHATRLQLCLFDPTGRKEIARLDLPECT
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEECCCCCHHHHHCCCHHHH
DEIWHGYLPGAHPGTVYAFRADGPYQPQYGHRFNSSKLLLDPYARKLIGQFRWSDALFGY
HHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCEEE
RLHSNRADLSIDRRDSAPAMPKCVVVDESFDWHDDRRPAVPWQDTVIYETHVRGVSMRRD
EEECCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHC
GLRTPERGTFAVLASPDFIDHLLRLGVTTVELLPVQAFLDSRELVNRGLRNYWGYDTLAY
CCCCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEE
FAPQPSYLATRRLDEMRIAIRQLHAAGIEVLLDVVYNHTCEGNQLGPTLSWRGLDNASYY
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCEE
RLQPNDPRYHIDETGCGNTLNLSHPRVLQMVMDSLRYWCTAFNIDGFRFDLGVTLGREPH
EECCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCEEEEEEEEECCCCC
GYDRGSGFFDALCQDPVLATRKLISEPWDLGPGGYQLGNHPAGFGEWNDKFRDTVRRFWR
CCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
GDAGMRPELAARLAGSADLFNHHRRKPWASVNFVTAHDGFTLADLVSYSSKRNEANGEEN
CCCCCCHHHHHHHCCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCC
RDGRDDNCSANWGVEGPTDDADIREVRERVARSMLTTLYAALGTPMLLGGDEFGRSQQGN
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHCCCCCCC
NNAYCQDTELAWFDWEAAASDAGRRLTGFVARLAALRREHPLLSGPGYPSGDREAAPGLR
CCCCEECCCEEEEECHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCH
EIDWFDERGEAIPVPAWQNRELRALMMRRVGPDRDGHTEVLLLMLNGSAQPLVFVPPAPR
HCCCHHCCCCEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEECCCHH
LAFRVLADSARPDLEPQPLPAEGIELAAHAAVIAAARLPAEQCTRPDHAGAGAAGEARPA
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCC
DEGDAPPDDADHAAQPQAAPAPQREPEPEPTAGPAPAHDEPTPVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRAATADRLEPGKPYPLGASWNGLGINFAVFSAHATRLQLCLFDPTGRKEIARLDLPECT
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEECCCCCHHHHHCCCHHHH
DEIWHGYLPGAHPGTVYAFRADGPYQPQYGHRFNSSKLLLDPYARKLIGQFRWSDALFGY
HHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCEEE
RLHSNRADLSIDRRDSAPAMPKCVVVDESFDWHDDRRPAVPWQDTVIYETHVRGVSMRRD
EEECCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHC
GLRTPERGTFAVLASPDFIDHLLRLGVTTVELLPVQAFLDSRELVNRGLRNYWGYDTLAY
CCCCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEE
FAPQPSYLATRRLDEMRIAIRQLHAAGIEVLLDVVYNHTCEGNQLGPTLSWRGLDNASYY
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCEE
RLQPNDPRYHIDETGCGNTLNLSHPRVLQMVMDSLRYWCTAFNIDGFRFDLGVTLGREPH
EECCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCEEEEEEEEECCCCC
GYDRGSGFFDALCQDPVLATRKLISEPWDLGPGGYQLGNHPAGFGEWNDKFRDTVRRFWR
CCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
GDAGMRPELAARLAGSADLFNHHRRKPWASVNFVTAHDGFTLADLVSYSSKRNEANGEEN
CCCCCCHHHHHHHCCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCC
RDGRDDNCSANWGVEGPTDDADIREVRERVARSMLTTLYAALGTPMLLGGDEFGRSQQGN
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHCCCCCCC
NNAYCQDTELAWFDWEAAASDAGRRLTGFVARLAALRREHPLLSGPGYPSGDREAAPGLR
CCCCEECCCEEEEECHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCH
EIDWFDERGEAIPVPAWQNRELRALMMRRVGPDRDGHTEVLLLMLNGSAQPLVFVPPAPR
HCCCHHCCCCEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEECCCHH
LAFRVLADSARPDLEPQPLPAEGIELAAHAAVIAAARLPAEQCTRPDHAGAGAAGEARPA
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCC
DEGDAPPDDADHAAQPQAAPAPQREPEPEPTAGPAPAHDEPTPVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]